Q9FMM0 (UBC18_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable ubiquitin-conjugating enzyme E2 18
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein 18

Gene names

Name:	UBC18
Ordered Locus Names:	At5g42990
ORF Names:	MBD2.19

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	161 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 161	161	Probable ubiquitin-conjugating enzyme E2 18		PRO_0000345184
Regions
	Compositional bias	3 – 8	6	Poly-Ser
Sites
	Active site	99	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9FMM0 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 04A773CB44F5F60E	FASTA	161	18,372
	10 20 30 40 50 60 MTSSSSPSRK ALSKIACNRL QKELSEWQVN PPTGFKHRVT DNLQKWVIEV TGAPGTLYAN 70 80 90 100 110 120 ETYNLQVEFP QHYPMEAPQV IFVPPAPLHP HIYSNGHICL DILYDSWSPA MTVSSVCISI 130 140 150 160 LSMLSSSPEK QRPTDNDRYV KNCKNGRSPK ETRWWFHDDK V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis." Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J. Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones." Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S. DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"A new family of ubiquitin-conjugating enzymes (E2S) AtUBC15/16/17/18 in Arabidopsis thaliana." Yan N., Doelling J., Vierstra R.D. Plant Gene Register PGR97-174 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 65-161. Strain: cv. Columbia.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ027032 mRNA. Translation: AAY44858.1. AB008264 Genomic DNA. Translation: BAB09201.1. CP002688 Genomic DNA. Translation: AED94898.1. AY058137 mRNA. Translation: AAL25553.1. AY101537 mRNA. Translation: AAM26658.1. AF028341 mRNA. Translation: AAC39327.1.
IPI	IPI00543321.
PIR	T52052.
RefSeq	NP_568619.1. NM_123665.5.
UniGene	At.6530.
3D structure databases
HSSP	HSSP built from PDB template 2A7L based on UniProtKB Q96B02.
ProteinModelPortal	Q9FMM0.
SMR	Q9FMM0. Positions 19-125.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9FMM0. 4 interactions.
Proteomic databases
PRIDE	Q9FMM0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G42990.1; AT5G42990.1; AT5G42990.
GeneID	834314.
KEGG	ath:AT5G42990.
Organism-specific databases
TAIR	At5g42990.
Phylogenomic databases
eggNOG	COG5078.
HOGENOM	HOG000233453.
InParanoid	Q9FMM0.
KO	K10688.
OMA	FTGDNIP.
PhylomeDB	Q9FMM0.
ProtClustDB	CLSN2688481.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Genevestigator	Q9FMM0.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBC18_ARATH

Accession

Primary (citable) accession number: Q9FMM0
Secondary accession number(s): O48954

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program