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Q9FMM0 (UBC18_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable ubiquitin-conjugating enzyme E2 18

EC=6.3.2.19
Alternative name(s):
Ubiquitin carrier protein 18
Gene names
Name:UBC18
Ordered Locus Names:At5g42990
ORF Names:MBD2.19
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length161 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins By similarity.

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ubiquitin-protein transferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 161161Probable ubiquitin-conjugating enzyme E2 18
PRO_0000345184

Regions

Compositional bias3 – 86Poly-Ser

Sites

Active site991Glycyl thioester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9FMM0 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 04A773CB44F5F60E

FASTA16118,372
        10         20         30         40         50         60 
MTSSSSPSRK ALSKIACNRL QKELSEWQVN PPTGFKHRVT DNLQKWVIEV TGAPGTLYAN 

        70         80         90        100        110        120 
ETYNLQVEFP QHYPMEAPQV IFVPPAPLHP HIYSNGHICL DILYDSWSPA MTVSSVCISI 

       130        140        150        160 
LSMLSSSPEK QRPTDNDRYV KNCKNGRSPK ETRWWFHDDK V 

« Hide

References

« Hide 'large scale' references
[1]"Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"A new family of ubiquitin-conjugating enzymes (E2S) AtUBC15/16/17/18 in Arabidopsis thaliana."
Yan N., Doelling J., Vierstra R.D.
Plant Gene Register PGR97-174
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 65-161.
Strain: cv. Columbia.
+Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ027032 mRNA. Translation: AAY44858.1.
AB008264 Genomic DNA. Translation: BAB09201.1.
CP002688 Genomic DNA. Translation: AED94898.1.
AY058137 mRNA. Translation: AAL25553.1.
AY101537 mRNA. Translation: AAM26658.1.
AF028341 mRNA. Translation: AAC39327.1.
PIRT52052.
RefSeqNP_568619.1. NM_123665.5.
UniGeneAt.6530.

3D structure databases

ProteinModelPortalQ9FMM0.
SMRQ9FMM0. Positions 19-125.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid19564. 4 interactions.
IntActQ9FMM0. 4 interactions.

Proteomic databases

PRIDEQ9FMM0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G42990.1; AT5G42990.1; AT5G42990.
GeneID834314.
KEGGath:AT5G42990.

Organism-specific databases

TAIRAT5G42990.

Phylogenomic databases

eggNOGCOG5078.
HOGENOMHOG000233453.
InParanoidQ9FMM0.
KOK10688.
OMAFTGDNIP.
PhylomeDBQ9FMM0.

Enzyme and pathway databases

BioCycARA:AT5G42990-MONOMER.
UniPathwayUPA00143.

Gene expression databases

GenevestigatorQ9FMM0.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBC18_ARATH
AccessionPrimary (citable) accession number: Q9FMM0
Secondary accession number(s): O48954
Entry history
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: March 1, 2001
Last modified: June 11, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names