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Protein

Probable inactive purple acid phosphatase 29

Gene

PAP29

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Cofactori

Protein has several cofactor binding sites:
  • Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi136 – 1361ZincBy similarity
Binding sitei136 – 1361SubstrateBy similarity
Metal bindingi303 – 3031ZincBy similarity
Metal bindingi305 – 3051IronBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: TAIR
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Ligandi

Iron, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Probable inactive purple acid phosphatase 29
Gene namesi
Name:PAP29
Ordered Locus Names:At5g63140
ORF Names:MDC12.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G63140.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3434Sequence AnalysisAdd
BLAST
Chaini35 – 389355Probable inactive purple acid phosphatase 29PRO_0000372831Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi80 – 801N-linked (GlcNAc...)Sequence Analysis
Glycosylationi191 – 1911N-linked (GlcNAc...)Sequence Analysis
Glycosylationi267 – 2671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi380 – 3801N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9FMK9.
PRIDEiQ9FMK9.

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, flowers and siliques.1 Publication

Gene expression databases

GenevestigatoriQ9FMK9.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT5G63140.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9FMK9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni303 – 3053Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1409.
HOGENOMiHOG000249829.
InParanoidiQ9FMK9.
OMAiQESTFTR.
PhylomeDBiQ9FMK9.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR011230. Pesterase_At2g46.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF030250. Ptase_At2g46880. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FMK9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADNRRRRSL FDFLLFSVFL GLACLCLSPI PATAQRRKLR FSVNGEFKIL
60 70 80 90 100
QVADMHFANG AKTQCQNVLP SQRAHCSDLN TTIFMSRVIA AEKPDLIVFT
110 120 130 140 150
GDNIFGFDVK DALKSINAAF APAIASKIPW VAILGNHDQE STFTRQQVMN
160 170 180 190 200
HIVKLPNTLS QVNPPEAAHY IDGFGNYNLQ IHGAADSKLQ NKSVLNLYFL
210 220 230 240 250
DSGDYSSVPY MEGYDWIKTS QQFWFDRTSK RLKREYNAKP NPQEGIAPGL
260 270 280 290 300
AYFHIPLPEF LSFDSKNATK GVRQEGTSAA STNSGFFTTL IARGDVKSVF
310 320 330 340 350
VGHDHVNDFC GELKGLNLCY GGGFGYHAYG KAGWERRARV VVVDLNKKRK
360 370 380
GKWGAVKSIK TWKRLDDKHL SVIDSQVLWN NSANKLVVR
Length:389
Mass (Da):43,548
Last modified:March 1, 2001 - v1
Checksum:i0E483ABCB10AF18A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY882861 mRNA. Translation: AAW80661.1.
AB008265 Genomic DNA. Translation: BAB10556.1.
CP002688 Genomic DNA. Translation: AED97710.1.
BT014894 mRNA. Translation: AAT44970.1.
BT015022 mRNA. Translation: AAT70473.1.
RefSeqiNP_201119.1. NM_125709.4.
UniGeneiAt.8836.

Genome annotation databases

EnsemblPlantsiAT5G63140.1; AT5G63140.1; AT5G63140.
GeneIDi836435.
KEGGiath:AT5G63140.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY882861 mRNA. Translation: AAW80661.1.
AB008265 Genomic DNA. Translation: BAB10556.1.
CP002688 Genomic DNA. Translation: AED97710.1.
BT014894 mRNA. Translation: AAT44970.1.
BT015022 mRNA. Translation: AAT70473.1.
RefSeqiNP_201119.1. NM_125709.4.
UniGeneiAt.8836.

3D structure databases

ProteinModelPortaliQ9FMK9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G63140.1-P.

Proteomic databases

PaxDbiQ9FMK9.
PRIDEiQ9FMK9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G63140.1; AT5G63140.1; AT5G63140.
GeneIDi836435.
KEGGiath:AT5G63140.

Organism-specific databases

TAIRiAT5G63140.

Phylogenomic databases

eggNOGiCOG1409.
HOGENOMiHOG000249829.
InParanoidiQ9FMK9.
OMAiQESTFTR.
PhylomeDBiQ9FMK9.

Gene expression databases

GenevestigatoriQ9FMK9.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR011230. Pesterase_At2g46.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF030250. Ptase_At2g46880. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
    Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
    Plant Mol. Biol. 59:581-594(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
    Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 4:401-414(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
    Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
    J. Biol. Chem. 277:27772-27781(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiPPA29_ARATH
AccessioniPrimary (citable) accession number: Q9FMK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: March 1, 2001
Last modified: January 7, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Lacks the conserved His residue essential for phosphatase activity. Its enzyme activity is therefore unsure.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.