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Q9FMD9

- GLN14_ARATH

UniProt

Q9FMD9 - GLN14_ARATH

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Protein
Glutamine synthetase cytosolic isozyme 1-4
Gene
GLN1-4, At5g16570, MTG13.1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

High-affinity glutamine synthetase. May contribute to the homeostatic control of glutamine synthesis in roots.

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Kineticsi

Measured at pH 7.8 and 30 degrees Celsius for all experiments.

  1. KM=0.6 mM for glutamate1 Publication
  2. KM=48 µM for ammonium
  3. KM=400 µM for ATP

Vmax=79.2 nmol/sec/mg enzyme with glutamate as substrate

Vmax=65.7 nmol/sec/mg enzyme with ammonium as substrate

Vmax=73.9 nmol/sec/mg enzyme with ATP as substrate

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: TAIR
Complete GO annotation...

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
  2. nitrate assimilation Source: TAIR
  3. nitrogen fixation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G16570-MONOMER.
MetaCyc:AT5G16570-MONOMER.
BRENDAi6.3.1.2. 399.
SABIO-RKQ9FMD9.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase cytosolic isozyme 1-4 (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase GLN1;4
Short name:
GLN1;4
Gene namesi
Name:GLN1-4
Ordered Locus Names:At5g16570
ORF Names:MTG13.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G16570.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi49 – 491Q → K: 6-fold decrease in affinity for ammonium and catalytic efficiency; when associated with A-174. 1 Publication
Mutagenesisi174 – 1741S → A: 6-fold decrease in affinity for ammonium and catalytic efficiency; when associated with K-49. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 356355Glutamine synthetase cytosolic isozyme 1-4
PRO_0000239820Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine By similarity
Modified residuei2 – 21Phosphoserine By similarity
Modified residuei48 – 481Phosphoserine By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9FMD9.
PRIDEiQ9FMD9.

Expressioni

Tissue specificityi

Expressed in the pericycle in the region of lateral root emergence.1 Publication

Inductioni

Down-regulated by ammonium supply.1 Publication

Gene expression databases

GenevestigatoriQ9FMD9.

Interactioni

Subunit structurei

Homooctamer By similarity.

Protein-protein interaction databases

BioGridi16795. 1 interaction.
STRINGi3702.AT5G16570.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9FMD9.
SMRiQ9FMD9. Positions 4-355.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0174.
HOGENOMiHOG000061500.
InParanoidiQ9FMD9.
KOiK01915.
OMAiHETSNIF.
PhylomeDBiQ9FMD9.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FMD9-1 [UniParc]FASTAAdd to Basket

« Hide

MSSLADLINL DLSDSTDQII AEYIWIGGSG LDMRSKARTL PGPVTDPSQL    50
PKWNYDGSST GQAPGDDSEV IIYPQAIFKD PFRRGNNILV MCDAYTPAGE 100
PIPTNKRHAA AKIFEDPSVV AEETWYGIEQ EYTLLQKDIK WPVGWPVGGF 150
PGPQGPYYCG VGADKAFGRD IVDSHYKACL YAGINVSGTN GEVMPGQWEF 200
QVGPTVGIAA ADQVWVARYI LERITELAGV VLSLDPKPIP GDWNGAGAHT 250
NYSTKSMRED GGYEVIKKAI EKLGLRHKEH IAAYGEGNER RLTGKHETAD 300
INTFLWGVAN RGASIRVGRD TEQAGKGYFE DRRPASNMDP YTVTSMIAES 350
TILWKP 356
Length:356
Mass (Da):38,987
Last modified:March 1, 2001 - v1
Checksum:iA8F39CE8835592D4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB008270 Genomic DNA. Translation: BAB10184.1.
CP002688 Genomic DNA. Translation: AED92312.1.
AY059932 mRNA. Translation: AAL24414.1.
AY128749 mRNA. Translation: AAM91149.1.
RefSeqiNP_568335.1. NM_121663.2.
UniGeneiAt.24254.

Genome annotation databases

EnsemblPlantsiAT5G16570.1; AT5G16570.1; AT5G16570.
GeneIDi831519.
KEGGiath:AT5G16570.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB008270 Genomic DNA. Translation: BAB10184.1 .
CP002688 Genomic DNA. Translation: AED92312.1 .
AY059932 mRNA. Translation: AAL24414.1 .
AY128749 mRNA. Translation: AAM91149.1 .
RefSeqi NP_568335.1. NM_121663.2.
UniGenei At.24254.

3D structure databases

ProteinModelPortali Q9FMD9.
SMRi Q9FMD9. Positions 4-355.
ModBasei Search...

Protein-protein interaction databases

BioGridi 16795. 1 interaction.
STRINGi 3702.AT5G16570.1-P.

Proteomic databases

PaxDbi Q9FMD9.
PRIDEi Q9FMD9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G16570.1 ; AT5G16570.1 ; AT5G16570 .
GeneIDi 831519.
KEGGi ath:AT5G16570.

Organism-specific databases

TAIRi AT5G16570.

Phylogenomic databases

eggNOGi COG0174.
HOGENOMi HOG000061500.
InParanoidi Q9FMD9.
KOi K01915.
OMAi HETSNIF.
PhylomeDBi Q9FMD9.

Enzyme and pathway databases

BioCyci ARA:AT5G16570-MONOMER.
MetaCyc:AT5G16570-MONOMER.
BRENDAi 6.3.1.2. 399.
SABIO-RK Q9FMD9.

Gene expression databases

Genevestigatori Q9FMD9.

Family and domain databases

Gene3Di 3.30.590.10. 1 hit.
InterProi IPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view ]
Pfami PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF54368. SSF54368. 1 hit.
PROSITEi PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
    Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Kinetic properties and ammonium-dependent regulation of cytosolic isoenzymes of glutamine synthetase in Arabidopsis."
    Ishiyama K., Inoue E., Watanabe-Takahashi A., Obara M., Yamaya T., Takahashi H.
    J. Biol. Chem. 279:16598-16605(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, INDUCTION.
  5. "Gln49 and Ser174 residues play critical roles in determining the catalytic efficiencies of plant glutamine synthetase."
    Ishiyama K., Inoue E., Yamaya T., Takahashi H.
    Plant Cell Physiol. 47:299-303(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF GLN-49 AND SER-174.

Entry informationi

Entry nameiGLN14_ARATH
AccessioniPrimary (citable) accession number: Q9FMD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: March 1, 2001
Last modified: May 14, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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