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Protein

Glutamine synthetase cytosolic isozyme 1-4

Gene

GLN1-4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High-affinity glutamine synthetase. May contribute to the homeostatic control of glutamine synthesis in roots.

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Kineticsi

Measured at pH 7.8 and 30 degrees Celsius for all experiments.

  1. KM=0.6 mM for glutamate1 Publication
  2. KM=48 µM for ammonium1 Publication
  3. KM=400 µM for ATP1 Publication
  1. Vmax=79.2 nmol/sec/mg enzyme with glutamate as substrate1 Publication
  2. Vmax=65.7 nmol/sec/mg enzyme with ammonium as substrate1 Publication
  3. Vmax=73.9 nmol/sec/mg enzyme with ATP as substrate1 Publication

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glutamate-ammonia ligase activity Source: TAIR

GO - Biological processi

  • glutamine biosynthetic process Source: InterPro
  • nitrate assimilation Source: TAIR
  • nitrogen fixation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G16570-MONOMER.
MetaCyc:AT5G16570-MONOMER.
BRENDAi6.3.1.2. 399.
ReactomeiREACT_276185. Amino acid synthesis and interconversion (transamination).
REACT_282855. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
SABIO-RKQ9FMD9.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase cytosolic isozyme 1-4 (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase GLN1;4
Short name:
GLN1;4
Gene namesi
Name:GLN1-4
Ordered Locus Names:At5g16570
ORF Names:MTG13.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G16570.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi49 – 491Q → K: 6-fold decrease in affinity for ammonium and catalytic efficiency; when associated with A-174. 1 Publication
Mutagenesisi174 – 1741S → A: 6-fold decrease in affinity for ammonium and catalytic efficiency; when associated with K-49. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 356355Glutamine synthetase cytosolic isozyme 1-4PRO_0000239820Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei2 – 21PhosphoserineBy similarity
Modified residuei48 – 481PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9FMD9.
PRIDEiQ9FMD9.

Expressioni

Tissue specificityi

Expressed in the pericycle in the region of lateral root emergence.1 Publication

Inductioni

Down-regulated by ammonium supply.1 Publication

Interactioni

Subunit structurei

Homooctamer (By similarity). Interacts with GRF3.By similarity1 Publication

Protein-protein interaction databases

BioGridi16795. 1 interaction.
STRINGi3702.AT5G16570.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FMD9.
SMRiQ9FMD9. Positions 4-355.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiCOG0174.
HOGENOMiHOG000061500.
InParanoidiQ9FMD9.
KOiK01915.
OMAiAESTILW.
PhylomeDBiQ9FMD9.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FMD9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLADLINL DLSDSTDQII AEYIWIGGSG LDMRSKARTL PGPVTDPSQL
60 70 80 90 100
PKWNYDGSST GQAPGDDSEV IIYPQAIFKD PFRRGNNILV MCDAYTPAGE
110 120 130 140 150
PIPTNKRHAA AKIFEDPSVV AEETWYGIEQ EYTLLQKDIK WPVGWPVGGF
160 170 180 190 200
PGPQGPYYCG VGADKAFGRD IVDSHYKACL YAGINVSGTN GEVMPGQWEF
210 220 230 240 250
QVGPTVGIAA ADQVWVARYI LERITELAGV VLSLDPKPIP GDWNGAGAHT
260 270 280 290 300
NYSTKSMRED GGYEVIKKAI EKLGLRHKEH IAAYGEGNER RLTGKHETAD
310 320 330 340 350
INTFLWGVAN RGASIRVGRD TEQAGKGYFE DRRPASNMDP YTVTSMIAES

TILWKP
Length:356
Mass (Da):38,987
Last modified:March 1, 2001 - v1
Checksum:iA8F39CE8835592D4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008270 Genomic DNA. Translation: BAB10184.1.
CP002688 Genomic DNA. Translation: AED92312.1.
AY059932 mRNA. Translation: AAL24414.1.
AY128749 mRNA. Translation: AAM91149.1.
RefSeqiNP_568335.1. NM_121663.2.
UniGeneiAt.24254.

Genome annotation databases

EnsemblPlantsiAT5G16570.1; AT5G16570.1; AT5G16570.
GeneIDi831519.
KEGGiath:AT5G16570.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008270 Genomic DNA. Translation: BAB10184.1.
CP002688 Genomic DNA. Translation: AED92312.1.
AY059932 mRNA. Translation: AAL24414.1.
AY128749 mRNA. Translation: AAM91149.1.
RefSeqiNP_568335.1. NM_121663.2.
UniGeneiAt.24254.

3D structure databases

ProteinModelPortaliQ9FMD9.
SMRiQ9FMD9. Positions 4-355.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi16795. 1 interaction.
STRINGi3702.AT5G16570.1.

Proteomic databases

PaxDbiQ9FMD9.
PRIDEiQ9FMD9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G16570.1; AT5G16570.1; AT5G16570.
GeneIDi831519.
KEGGiath:AT5G16570.

Organism-specific databases

TAIRiAT5G16570.

Phylogenomic databases

eggNOGiCOG0174.
HOGENOMiHOG000061500.
InParanoidiQ9FMD9.
KOiK01915.
OMAiAESTILW.
PhylomeDBiQ9FMD9.

Enzyme and pathway databases

BioCyciARA:AT5G16570-MONOMER.
MetaCyc:AT5G16570-MONOMER.
BRENDAi6.3.1.2. 399.
ReactomeiREACT_276185. Amino acid synthesis and interconversion (transamination).
REACT_282855. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
SABIO-RKQ9FMD9.

Miscellaneous databases

PROiQ9FMD9.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
    Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Kinetic properties and ammonium-dependent regulation of cytosolic isoenzymes of glutamine synthetase in Arabidopsis."
    Ishiyama K., Inoue E., Watanabe-Takahashi A., Obara M., Yamaya T., Takahashi H.
    J. Biol. Chem. 279:16598-16605(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, INDUCTION.
  5. "Gln49 and Ser174 residues play critical roles in determining the catalytic efficiencies of plant glutamine synthetase."
    Ishiyama K., Inoue E., Yamaya T., Takahashi H.
    Plant Cell Physiol. 47:299-303(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF GLN-49 AND SER-174.
  6. "14-3-3 proteins fine-tune plant nutrient metabolism."
    Shin R., Jez J.M., Basra A., Zhang B., Schachtman D.P.
    FEBS Lett. 585:143-147(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GRF3.

Entry informationi

Entry nameiGLN14_ARATH
AccessioniPrimary (citable) accession number: Q9FMD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: March 1, 2001
Last modified: June 24, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.