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Q9FMD9

- GLN14_ARATH

UniProt

Q9FMD9 - GLN14_ARATH

Protein

Glutamine synthetase cytosolic isozyme 1-4

Gene

GLN1-4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    High-affinity glutamine synthetase. May contribute to the homeostatic control of glutamine synthesis in roots.

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    Kineticsi

    Measured at pH 7.8 and 30 degrees Celsius for all experiments.

    1. KM=0.6 mM for glutamate1 Publication
    2. KM=48 µM for ammonium1 Publication
    3. KM=400 µM for ATP1 Publication

    Vmax=79.2 nmol/sec/mg enzyme with glutamate as substrate1 Publication

    Vmax=65.7 nmol/sec/mg enzyme with ammonium as substrate1 Publication

    Vmax=73.9 nmol/sec/mg enzyme with ATP as substrate1 Publication

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-ammonia ligase activity Source: TAIR

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro
    2. nitrate assimilation Source: TAIR
    3. nitrogen fixation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Nitrogen fixation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G16570-MONOMER.
    MetaCyc:AT5G16570-MONOMER.
    BRENDAi6.3.1.2. 399.
    SABIO-RKQ9FMD9.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase cytosolic isozyme 1-4 (EC:6.3.1.2)
    Alternative name(s):
    Glutamate--ammonia ligase GLN1;4
    Short name:
    GLN1;4
    Gene namesi
    Name:GLN1-4
    Ordered Locus Names:At5g16570
    ORF Names:MTG13.1
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G16570.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi49 – 491Q → K: 6-fold decrease in affinity for ammonium and catalytic efficiency; when associated with A-174. 1 Publication
    Mutagenesisi174 – 1741S → A: 6-fold decrease in affinity for ammonium and catalytic efficiency; when associated with K-49. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 356355Glutamine synthetase cytosolic isozyme 1-4PRO_0000239820Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei2 – 21PhosphoserineBy similarity
    Modified residuei48 – 481PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ9FMD9.
    PRIDEiQ9FMD9.

    Expressioni

    Tissue specificityi

    Expressed in the pericycle in the region of lateral root emergence.1 Publication

    Inductioni

    Down-regulated by ammonium supply.1 Publication

    Gene expression databases

    GenevestigatoriQ9FMD9.

    Interactioni

    Subunit structurei

    Homooctamer.By similarity

    Protein-protein interaction databases

    BioGridi16795. 1 interaction.
    STRINGi3702.AT5G16570.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FMD9.
    SMRiQ9FMD9. Positions 4-355.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Phylogenomic databases

    eggNOGiCOG0174.
    HOGENOMiHOG000061500.
    InParanoidiQ9FMD9.
    KOiK01915.
    OMAiHETSNIF.
    PhylomeDBiQ9FMD9.

    Family and domain databases

    Gene3Di3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9FMD9-1 [UniParc]FASTAAdd to Basket

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    MSSLADLINL DLSDSTDQII AEYIWIGGSG LDMRSKARTL PGPVTDPSQL    50
    PKWNYDGSST GQAPGDDSEV IIYPQAIFKD PFRRGNNILV MCDAYTPAGE 100
    PIPTNKRHAA AKIFEDPSVV AEETWYGIEQ EYTLLQKDIK WPVGWPVGGF 150
    PGPQGPYYCG VGADKAFGRD IVDSHYKACL YAGINVSGTN GEVMPGQWEF 200
    QVGPTVGIAA ADQVWVARYI LERITELAGV VLSLDPKPIP GDWNGAGAHT 250
    NYSTKSMRED GGYEVIKKAI EKLGLRHKEH IAAYGEGNER RLTGKHETAD 300
    INTFLWGVAN RGASIRVGRD TEQAGKGYFE DRRPASNMDP YTVTSMIAES 350
    TILWKP 356
    Length:356
    Mass (Da):38,987
    Last modified:March 1, 2001 - v1
    Checksum:iA8F39CE8835592D4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB008270 Genomic DNA. Translation: BAB10184.1.
    CP002688 Genomic DNA. Translation: AED92312.1.
    AY059932 mRNA. Translation: AAL24414.1.
    AY128749 mRNA. Translation: AAM91149.1.
    RefSeqiNP_568335.1. NM_121663.2.
    UniGeneiAt.24254.

    Genome annotation databases

    EnsemblPlantsiAT5G16570.1; AT5G16570.1; AT5G16570.
    GeneIDi831519.
    KEGGiath:AT5G16570.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB008270 Genomic DNA. Translation: BAB10184.1 .
    CP002688 Genomic DNA. Translation: AED92312.1 .
    AY059932 mRNA. Translation: AAL24414.1 .
    AY128749 mRNA. Translation: AAM91149.1 .
    RefSeqi NP_568335.1. NM_121663.2.
    UniGenei At.24254.

    3D structure databases

    ProteinModelPortali Q9FMD9.
    SMRi Q9FMD9. Positions 4-355.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 16795. 1 interaction.
    STRINGi 3702.AT5G16570.1-P.

    Proteomic databases

    PaxDbi Q9FMD9.
    PRIDEi Q9FMD9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G16570.1 ; AT5G16570.1 ; AT5G16570 .
    GeneIDi 831519.
    KEGGi ath:AT5G16570.

    Organism-specific databases

    TAIRi AT5G16570.

    Phylogenomic databases

    eggNOGi COG0174.
    HOGENOMi HOG000061500.
    InParanoidi Q9FMD9.
    KOi K01915.
    OMAi HETSNIF.
    PhylomeDBi Q9FMD9.

    Enzyme and pathway databases

    BioCyci ARA:AT5G16570-MONOMER.
    MetaCyc:AT5G16570-MONOMER.
    BRENDAi 6.3.1.2. 399.
    SABIO-RK Q9FMD9.

    Gene expression databases

    Genevestigatori Q9FMD9.

    Family and domain databases

    Gene3Di 3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
      Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
      DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Kinetic properties and ammonium-dependent regulation of cytosolic isoenzymes of glutamine synthetase in Arabidopsis."
      Ishiyama K., Inoue E., Watanabe-Takahashi A., Obara M., Yamaya T., Takahashi H.
      J. Biol. Chem. 279:16598-16605(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, INDUCTION.
    5. "Gln49 and Ser174 residues play critical roles in determining the catalytic efficiencies of plant glutamine synthetase."
      Ishiyama K., Inoue E., Yamaya T., Takahashi H.
      Plant Cell Physiol. 47:299-303(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF GLN-49 AND SER-174.

    Entry informationi

    Entry nameiGLN14_ARATH
    AccessioniPrimary (citable) accession number: Q9FMD9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 13, 2006
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3