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Protein

Protein TIC 40, chloroplastic

Gene

TIC40

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in protein precursor import into chloroplasts. Part of the motor complex consisting of a co-chaperone (TIC40) and a chaperone (HSP93) associated with the import channel (TIC110). Causes the release of bound transit peptides from TIC110 and stimulates ATP hydrolysis by HSP93. Involved in reinsertion of proteins from the chloroplast stroma into the inner membrane.6 Publications

GO - Biological processi

  • chloroplast organization Source: TAIR
  • protein import into chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Protein family/group databases

TCDBi3.A.9.1.2. the chloroplast envelope protein translocase (cept or tic-toc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein TIC 40, chloroplastic
Alternative name(s):
Protein PIGMENT DEFECTIVE EMBRYO 120
Translocon at the inner envelope membrane of chloroplasts 40
Short name:
AtTIC40
Gene namesi
Name:TIC40
Synonyms:PDE120
Ordered Locus Names:At5g16620
ORF Names:MTG13.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G16620.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini77 – 104Chloroplast intermembraneSequence analysisAdd BLAST28
Transmembranei105 – 125HelicalSequence analysisAdd BLAST21
Topological domaini126 – 447StromalSequence analysisAdd BLAST322

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast inner membrane Source: TAIR
  • chloroplast thylakoid membrane Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: TAIR
  • Tic complex Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Plastid inner membrane

Pathology & Biotechi

Disruption phenotypei

Small and chlorotic, but not seedling lethal. Defective in chloroplast protein import.3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi396N → A: Loss of stimulation of HSP93 ATP hydrolysis. 1 Publication1
Mutagenesisi405N → A: Loss of stimulation of HSP93 ATP hydrolysis. 1 Publication1
Mutagenesisi418N → A: No effect on stimulation of HSP93 ATP hydrolysis. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 43ChloroplastSequence analysisAdd BLAST43
Transit peptidei44 – 76Chloroplast; inner membraneSequence analysisAdd BLAST33
ChainiPRO_000041367377 – 447Protein TIC 40, chloroplasticAdd BLAST371

Proteomic databases

PaxDbiQ9FMD5.
PRIDEiQ9FMD5.

PTM databases

iPTMnetiQ9FMD5.

Expressioni

Tissue specificityi

Expressed in seedlings, flowers, leaves, stems and roots.2 Publications

Developmental stagei

Expressed throughout development.1 Publication

Gene expression databases

GenevisibleiQ9FMD5. AT.

Interactioni

Subunit structurei

Part of the Tic complex. Interacts with HSP93, TIC110 and LTD.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TIC110Q8LPR93EBI-639157,EBI-639092

Protein-protein interaction databases

BioGridi16800. 7 interactors.
IntActiQ9FMD5. 2 interactors.
STRINGi3702.AT5G16620.1.

Structurei

Secondary structure

1447
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi387 – 393Combined sources7
Helixi397 – 402Combined sources6
Helixi406 – 415Combined sources10
Helixi419 – 421Combined sources3
Helixi422 – 425Combined sources4
Helixi429 – 441Combined sources13
Beta strandi442 – 444Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LNMNMR-A386-447[»]
ProteinModelPortaliQ9FMD5.
SMRiQ9FMD5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini310 – 344STI1 1Add BLAST35
Domaini386 – 425STI1 2Add BLAST40

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi44 – 107Ser-richAdd BLAST64

Domaini

The C-terminal half (89-105) of the Ser/Pro-rich region and the transmembrane domain are necessary and sufficient for membrane integration.
The TPR region (238-373) interacts with TIC110.
The STI1 2 domain (386-425) has a stimulatory effect on HSP93 ATP hydrolysis.

Sequence similaritiesi

Contains 2 STI1 domains.Curated

Keywords - Domaini

Repeat, Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IN5R. Eukaryota.
ENOG410XSN5. LUCA.
HOGENOMiHOG000082622.
InParanoidiQ9FMD5.
OMAiTTESRPM.
OrthoDBiEOG09360KP0.
PhylomeDBiQ9FMD5.

Family and domain databases

InterProiIPR006636. STI1_HS-bd.
[Graphical view]
SMARTiSM00727. STI1. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FMD5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENLTLVSCS ASSPKLLIGC NFTSSLKNPT GFSRRTPNIV LRCSKISASA
60 70 80 90 100
QSQSPSSRPE NTGEIVVVKQ RSKAFASIFS SSRDQQTTSV ASPSVPVPPP
110 120 130 140 150
SSSTIGSPLF WIGVGVGLSA LFSYVTSNLK KYAMQTAMKT MMNQMNTQNS
160 170 180 190 200
QFNNSGFPSG SPFPFPFPPQ TSPASSPFQS QSQSSGATVD VTATKVETPP
210 220 230 240 250
STKPKPTPAK DIEVDKPSVV LEASKEKKEE KNYAFEDISP EETTKESPFS
260 270 280 290 300
NYAEVSETNS PKETRLFEDV LQNGAGPANG ATASEVFQSL GGGKGGPGLS
310 320 330 340 350
VEALEKMMED PTVQKMVYPY LPEEMRNPET FKWMLKNPQY RQQLQDMLNN
360 370 380 390 400
MSGSGEWDKR MTDTLKNFDL NSPEVKQQFN QIGLTPEEVI SKIMENPDVA
410 420 430 440
MAFQNPRVQA ALMECSENPM NIMKYQNDKE VMDVFNKISQ LFPGMTG
Length:447
Mass (Da):48,903
Last modified:March 1, 2001 - v1
Checksum:i52D3E7222AB461CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008270 Genomic DNA. Translation: BAB10189.1.
CP002688 Genomic DNA. Translation: AED92318.1.
AF428299 mRNA. Translation: AAL16131.1.
AY093010 mRNA. Translation: AAM13009.1.
BT006595 mRNA. Translation: AAP31939.1.
RefSeqiNP_197165.1. NM_121668.4.
UniGeneiAt.25049.
At.57178.

Genome annotation databases

EnsemblPlantsiAT5G16620.1; AT5G16620.1; AT5G16620.
GeneIDi831524.
GrameneiAT5G16620.1; AT5G16620.1; AT5G16620.
KEGGiath:AT5G16620.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008270 Genomic DNA. Translation: BAB10189.1.
CP002688 Genomic DNA. Translation: AED92318.1.
AF428299 mRNA. Translation: AAL16131.1.
AY093010 mRNA. Translation: AAM13009.1.
BT006595 mRNA. Translation: AAP31939.1.
RefSeqiNP_197165.1. NM_121668.4.
UniGeneiAt.25049.
At.57178.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LNMNMR-A386-447[»]
ProteinModelPortaliQ9FMD5.
SMRiQ9FMD5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi16800. 7 interactors.
IntActiQ9FMD5. 2 interactors.
STRINGi3702.AT5G16620.1.

Protein family/group databases

TCDBi3.A.9.1.2. the chloroplast envelope protein translocase (cept or tic-toc) family.

PTM databases

iPTMnetiQ9FMD5.

Proteomic databases

PaxDbiQ9FMD5.
PRIDEiQ9FMD5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G16620.1; AT5G16620.1; AT5G16620.
GeneIDi831524.
GrameneiAT5G16620.1; AT5G16620.1; AT5G16620.
KEGGiath:AT5G16620.

Organism-specific databases

TAIRiAT5G16620.

Phylogenomic databases

eggNOGiENOG410IN5R. Eukaryota.
ENOG410XSN5. LUCA.
HOGENOMiHOG000082622.
InParanoidiQ9FMD5.
OMAiTTESRPM.
OrthoDBiEOG09360KP0.
PhylomeDBiQ9FMD5.

Miscellaneous databases

PROiQ9FMD5.

Gene expression databases

GenevisibleiQ9FMD5. AT.

Family and domain databases

InterProiIPR006636. STI1_HS-bd.
[Graphical view]
SMARTiSM00727. STI1. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTIC40_ARATH
AccessioniPrimary (citable) accession number: Q9FMD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Inserts into the inner envelope membrane from the stroma after import from the cytoplasm. The transit peptide undergoes a two-step processing. The initial cleavage to generate the intermediate found in the stroma is mediated by the stromal processing peptidase (SPP) while the final processing step by a signal peptidase I-type (SPase I), possibly PLSP1, requires association with the inner membrane.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.