Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9FMD5 (TIC40_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein TIC 40, chloroplastic
Alternative name(s):
Protein PIGMENT DEFECTIVE EMBRYO 120
Translocon at the inner envelope membrane of chloroplasts 40
Short name=AtTIC40
Gene names
Name:TIC40
Synonyms:PDE120
Ordered Locus Names:At5g16620
ORF Names:MTG13.6
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length447 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in protein precursor import into chloroplasts. Part of the motor complex consisting of a co-chaperone (TIC40) and a chaperone (HSP93) associated with the import channel (TIC110). Causes the release of bound transit peptides from TIC110 and stimulates ATP hydrolysis by HSP93. Involved in reinsertion of proteins from the chloroplast stroma into the inner membrane. Ref.4 Ref.6 Ref.7 Ref.9 Ref.10 Ref.12

Subunit structure

Part of the Tic complex. Interacts with HSP93, TIC110 and LTD. Ref.6 Ref.9 Ref.10 Ref.13

Subcellular location

Plastidchloroplast inner membrane; Single-pass membrane protein Ref.4 Ref.8 Ref.10 Ref.11.

Tissue specificity

Expressed in seedlings, flowers, leaves, stems and roots. Ref.5 Ref.7

Developmental stage

Expressed throughout development. Ref.7

Domain

The C-terminal half (89-105) of the Ser/Pro-rich region and the transmembrane domain are necessary and sufficient for membrane integration.

The TPR region (238-373) interacts with TIC110.

The STI1 2 domain (386-425) has a stimulatory effect on HSP93 ATP hydrolysis.

Disruption phenotype

Small and chlorotic, but not seedling lethal. Defective in chloroplast protein import. Ref.4 Ref.7 Ref.10

Miscellaneous

Inserts into the inner envelope membrane from the stroma after import from the cytoplasm. The transit peptide undergoes a two-step processing. The initial cleavage to generate the intermediate found in the stroma is mediated by the stromal processing peptidase (SPP) while the final processing step by a signal peptidase I-type (SPase I), possibly PLSP1, requires association with the inner membrane.

Sequence similarities

Contains 2 STI1 domains.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

TIC110Q8LPR93EBI-639157,EBI-639092

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4343Chloroplast Potential
Transit peptide44 – 7633Chloroplast; inner membrane Potential
Chain77 – 447371Protein TIC 40, chloroplastic
PRO_0000413673

Regions

Topological domain77 – 10428Chloroplast intermembrane Potential
Transmembrane105 – 12521Helical; Potential
Topological domain126 – 447322Stromal Potential
Domain310 – 34435STI1 1
Domain386 – 42540STI1 2
Compositional bias44 – 10764Ser-rich

Experimental info

Mutagenesis3961N → A: Loss of stimulation of HSP93 ATP hydrolysis. Ref.9
Mutagenesis4051N → A: Loss of stimulation of HSP93 ATP hydrolysis. Ref.9
Mutagenesis4181N → A: No effect on stimulation of HSP93 ATP hydrolysis. Ref.9

Secondary structure

............. 447
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9FMD5 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 52D3E7222AB461CE

FASTA44748,903
        10         20         30         40         50         60 
MENLTLVSCS ASSPKLLIGC NFTSSLKNPT GFSRRTPNIV LRCSKISASA QSQSPSSRPE 

        70         80         90        100        110        120 
NTGEIVVVKQ RSKAFASIFS SSRDQQTTSV ASPSVPVPPP SSSTIGSPLF WIGVGVGLSA 

       130        140        150        160        170        180 
LFSYVTSNLK KYAMQTAMKT MMNQMNTQNS QFNNSGFPSG SPFPFPFPPQ TSPASSPFQS 

       190        200        210        220        230        240 
QSQSSGATVD VTATKVETPP STKPKPTPAK DIEVDKPSVV LEASKEKKEE KNYAFEDISP 

       250        260        270        280        290        300 
EETTKESPFS NYAEVSETNS PKETRLFEDV LQNGAGPANG ATASEVFQSL GGGKGGPGLS 

       310        320        330        340        350        360 
VEALEKMMED PTVQKMVYPY LPEEMRNPET FKWMLKNPQY RQQLQDMLNN MSGSGEWDKR 

       370        380        390        400        410        420 
MTDTLKNFDL NSPEVKQQFN QIGLTPEEVI SKIMENPDVA MAFQNPRVQA ALMECSENPM 

       430        440 
NIMKYQNDKE VMDVFNKISQ LFPGMTG 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Tic40, a membrane-anchored co-chaperone homolog in the chloroplast protein translocon."
Chou M.L., Fitzpatrick L.M., Tu S.L., Budziszewski G., Potter-Lewis S., Akita M., Levin J.Z., Keegstra K., Li H.M.
EMBO J. 22:2970-2980(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, DISRUPTION PHENOTYPE.
[5]"The protein translocon of the plastid envelopes."
Vojta A., Alavi M., Becker T., Hoermann F., Kuechler M., Soll J., Thomson R., Schleiff E.
J. Biol. Chem. 279:21401-21405(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"Arabidopsis tic110 is essential for the assembly and function of the protein import machinery of plastids."
Inaba T., Alvarez-Huerta M., Li M., Bauer J., Ewers C., Kessler F., Schnell D.J.
Plant Cell 17:1482-1496(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH TIC110.
[7]"In vivo studies on the roles of Tic110, Tic40 and Hsp93 during chloroplast protein import."
Kovacheva S., Bedard J., Patel R., Dudley P., Twell D., Rios G., Koncz C., Jarvis P.
Plant J. 41:412-428(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
[8]"Reconstitution of protein targeting to the inner envelope membrane of chloroplasts."
Li M., Schnell D.J.
J. Cell Biol. 175:249-259(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, PROTEOLYTIC PROCESSING, TOPOLOGY.
[9]"Stimulation of transit-peptide release and ATP hydrolysis by a cochaperone during protein import into chloroplasts."
Chou M.L., Chu C.C., Chen L.J., Akita M., Li H.M.
J. Cell Biol. 175:893-900(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH TIC110 AND HSP93, MUTAGENESIS OF ASN-396; ASN-405 AND ASN-418.
[10]"Functional similarity between the chloroplast translocon component, Tic40, and the human co-chaperone, Hsp70-interacting protein (Hip)."
Bedard J., Kubis S., Bimanadham S., Jarvis P.
J. Biol. Chem. 282:21404-21414(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH TIC110, DISRUPTION PHENOTYPE.
[11]"A novel serine/proline-rich domain in combination with a transmembrane domain is required for the insertion of AtTic40 into the inner envelope membrane of chloroplasts."
Tripp J., Inoue K., Keegstra K., Froehlich J.E.
Plant J. 52:824-838(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, PROTEOLYTIC PROCESSING.
[12]"Tic40 is important for reinsertion of proteins from the chloroplast stroma into the inner membrane."
Chiu C.C., Li H.M.
Plant J. 56:793-801(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[13]"LTD is a protein required for sorting light-harvesting chlorophyll-binding proteins to the chloroplast SRP pathway."
Ouyang M., Li X., Ma J., Chi W., Xiao J., Zou M., Chen F., Lu C., Zhang L.
Nat. Commun. 2:277-277(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH LTD.
[14]"Protein import into chloroplasts: the Tic complex and its regulation."
Kovacs-Bogdan E., Soll J., Bolter B.
Biochim. Biophys. Acta 1803:740-747(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB008270 Genomic DNA. Translation: BAB10189.1.
CP002688 Genomic DNA. Translation: AED92318.1.
AF428299 mRNA. Translation: AAL16131.1.
AY093010 mRNA. Translation: AAM13009.1.
BT006595 mRNA. Translation: AAP31939.1.
RefSeqNP_197165.1. NM_121668.3.
UniGeneAt.25049.
At.57178.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2LNMNMR-A386-447[»]
ProteinModelPortalQ9FMD5.
SMRQ9FMD5. Positions 301-349, 386-447.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid16800. 5 interactions.
IntActQ9FMD5. 2 interactions.
STRING3702.AT5G16620.1-P.

Protein family/group databases

TCDB3.A.9.1.2. the chloroplast envelope protein translocase (cept or tic-toc) family.

Proteomic databases

PaxDbQ9FMD5.
PRIDEQ9FMD5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G16620.1; AT5G16620.1; AT5G16620.
GeneID831524.
KEGGath:AT5G16620.

Organism-specific databases

TAIRAT5G16620.

Phylogenomic databases

eggNOGNOG276582.
HOGENOMHOG000082622.
InParanoidQ9FMD5.
OMAPDVAMAF.
PhylomeDBQ9FMD5.
ProtClustDBCLSN2694805.

Gene expression databases

GenevestigatorQ9FMD5.

Family and domain databases

InterProIPR006636. STI1_HS-bd.
[Graphical view]
SMARTSM00727. STI1. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTIC40_ARATH
AccessionPrimary (citable) accession number: Q9FMD5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names