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Protein

Cytochrome P450 85A1

Gene

CYP85A1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the C6-oxidation step in brassinosteroids biosynthesis. Converts 6-deoxocastasterone to castasterone. May also convert 6-deoxoteasterone to teasterone, 3-dehydro-6-deoxoteasterone to 3-dehydroteasterone, and 6-deoxotyphasterol to typhasterol.2 Publications

Cofactori

hemeBy similarity

Pathwayi: brassinosteroid biosynthesis

This protein is involved in the pathway brassinosteroid biosynthesis, which is part of Plant hormone biosynthesis.
View all proteins of this organism that are known to be involved in the pathway brassinosteroid biosynthesis and in Plant hormone biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi415Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

  • brassinosteroid biosynthetic process Source: TAIR
  • brassinosteroid homeostasis Source: TAIR
  • multicellular organism development Source: GO_Central
  • sterol metabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Brassinosteroid biosynthesis, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G38970-MONOMER.
MetaCyc:AT5G38970-MONOMER.
ReactomeiR-ATH-211916. Vitamins.
R-ATH-5365859. RA biosynthesis pathway.
UniPathwayiUPA00381.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 85A1 (EC:1.14.-.-)
Alternative name(s):
Brassinosteroid-6-oxidase 1
Short name:
BR6ox 1
C6-oxidase 1
Gene namesi
Name:CYP85A1
Synonyms:BR6OX1
Ordered Locus Names:At5g38970
ORF Names:K15E6.150
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G38970.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei2 – 22HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000521691 – 465Cytochrome P450 85A1Add BLAST465

Proteomic databases

PaxDbiQ9FMA5.
PRIDEiQ9FMA5.

Expressioni

Tissue specificityi

Mainly expressed in apical shoots, siliques and roots.1 Publication

Inductioni

Repressed by brassinolide treatment.1 Publication

Gene expression databases

GenevisibleiQ9FMA5. AT.

Interactioni

Protein-protein interaction databases

BioGridi19140. 15 interactors.
MINTiMINT-8070888.
STRINGi3702.AT5G38970.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FMA5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000237614.
InParanoidiQ9FMA5.
KOiK09590.
OMAiHLRISPY.
OrthoDBiEOG093608LM.
PhylomeDBiQ9FMA5.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9FMA5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGAMMVMMGL LLIIVSLCSA LLRWNQMRYT KNGLPPGTMG WPIFGETTEF
60 70 80 90 100
LKQGPNFMRN QRLRYGSFFK SHLLGCPTLI SMDSEVNRYI LKNESKGLVP
110 120 130 140 150
GYPQSMLDIL GTCNMAAVHG SSHRLMRGSL LSLISSTMMR DHILPKVDHF
160 170 180 190 200
MRSYLDQWNE LEVIDIQDKT KHMAFLSSLT QIAGNLRKPF VEEFKTAFFK
210 220 230 240 250
LVVGTLSVPI DLPGTNYRCG IQARNNIDRL LRELMQERRD SGETFTDMLG
260 270 280 290 300
YLMKKEGNRY PLTDEEIRDQ VVTILYSGYE TVSTTSMMAL KYLHDHPKAL
310 320 330 340 350
QELRAEHLAF RERKRQDEPL GLEDVKSMKF TRAVIYETSR LATIVNGVLR
360 370 380 390 400
KTTRDLEING YLIPKGWRIY VYTREINYDA NLYEDPLIFN PWRWMKKSLE
410 420 430 440 450
SQNSCFVFGG GTRLCPGKEL GIVEISSFLH YFVTRYRWEE IGGDELMVFP
460
RVFAPKGFHL RISPY
Length:465
Mass (Da):53,767
Last modified:March 1, 2001 - v1
Checksum:i0C00459C9C866D1F
GO
Isoform 2 (identifier: Q9FMA5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-81: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:384
Mass (Da):44,584
Checksum:iAB74097E86B72070
GO
Isoform 3 (identifier: Q9FMA5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     396-419: KKSLESQNSCFVFGGGTRLCPGKE → VSPWSHKTHALCLEVGQGFVLVRN
     420-465: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:419
Mass (Da):48,416
Checksum:i62B0338B3714E0EC
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0144511 – 81Missing in isoform 2. CuratedAdd BLAST81
Alternative sequenceiVSP_014452396 – 419KKSLE…CPGKE → VSPWSHKTHALCLEVGQGFV LVRN in isoform 3. CuratedAdd BLAST24
Alternative sequenceiVSP_014453420 – 465Missing in isoform 3. CuratedAdd BLAST46

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB035868 mRNA. Translation: BAB60858.1.
AB009048 Genomic DNA. Translation: BAB08653.1.
CP002688 Genomic DNA. Translation: AED94380.1.
CP002688 Genomic DNA. Translation: AED94381.1.
CP002688 Genomic DNA. Translation: AED94382.1.
RefSeqiNP_198713.3. NM_123259.4. [Q9FMA5-2]
NP_851105.1. NM_180774.2. [Q9FMA5-1]
NP_974862.1. NM_203133.2. [Q9FMA5-3]
UniGeneiAt.47476.

Genome annotation databases

EnsemblPlantsiAT5G38970.1; AT5G38970.1; AT5G38970. [Q9FMA5-1]
GeneIDi833889.
GrameneiAT5G38970.1; AT5G38970.1; AT5G38970.
KEGGiath:AT5G38970.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB035868 mRNA. Translation: BAB60858.1.
AB009048 Genomic DNA. Translation: BAB08653.1.
CP002688 Genomic DNA. Translation: AED94380.1.
CP002688 Genomic DNA. Translation: AED94381.1.
CP002688 Genomic DNA. Translation: AED94382.1.
RefSeqiNP_198713.3. NM_123259.4. [Q9FMA5-2]
NP_851105.1. NM_180774.2. [Q9FMA5-1]
NP_974862.1. NM_203133.2. [Q9FMA5-3]
UniGeneiAt.47476.

3D structure databases

ProteinModelPortaliQ9FMA5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19140. 15 interactors.
MINTiMINT-8070888.
STRINGi3702.AT5G38970.1.

Proteomic databases

PaxDbiQ9FMA5.
PRIDEiQ9FMA5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G38970.1; AT5G38970.1; AT5G38970. [Q9FMA5-1]
GeneIDi833889.
GrameneiAT5G38970.1; AT5G38970.1; AT5G38970.
KEGGiath:AT5G38970.

Organism-specific databases

TAIRiAT5G38970.

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000237614.
InParanoidiQ9FMA5.
KOiK09590.
OMAiHLRISPY.
OrthoDBiEOG093608LM.
PhylomeDBiQ9FMA5.

Enzyme and pathway databases

UniPathwayiUPA00381.
BioCyciARA:AT5G38970-MONOMER.
MetaCyc:AT5G38970-MONOMER.
ReactomeiR-ATH-211916. Vitamins.
R-ATH-5365859. RA biosynthesis pathway.

Miscellaneous databases

PROiQ9FMA5.

Gene expression databases

GenevisibleiQ9FMA5. AT.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiC85A1_ARATH
AccessioniPrimary (citable) accession number: Q9FMA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.