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Protein

Pyruvate kinase

Gene

MCD7.8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase GAPC2, cytosolic (GAPC2), Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic (GAPC1)
  2. Phosphoglycerate kinase 3, cytosolic (PGK3)
  3. Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2 (At3g08590), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 (PGM1)
  4. Enolase 1, chloroplastic (ENO1), Cytosolic enolase 3 (ENO3), Bifunctional enolase 2/transcriptional activator (ENO2)
  5. Pyruvate kinase (AXX17_At3g24740), Pyruvate kinase (AXX17_At5g51800), Pyruvate kinase (At5g63680), Pyruvate kinase (AXX17_At3g50280), Pyruvate kinase (AXX17_ATUG04870), Pyruvate kinase (MAH20.13), Pyruvate kinase (MCD7.8), Pyruvate kinase (At3g25960), Pyruvate kinase (F1I16_60), Pyruvate kinase (MBK5.16), Pyruvate kinase (AXX17_At3g50440), Plastidial pyruvate kinase 3, chloroplastic (PKP3), Pyruvate kinase (At5g08570), Pyruvate kinase (At3g52990), Pyruvate kinase (At5g56350), Pyruvate kinase (AXX17_At3g28070), Pyruvate kinase (AXX17_At3g47400), Pyruvate kinase (At2g36580), Pyruvate kinase, Probable pyruvate kinase, cytosolic isozyme (At4g26390), Pyruvate kinase (F8J2_160), Pyruvate kinase (At3g52990), Pyruvate kinase (AXX17_At3g03460), Plastidial pyruvate kinase 2 (PKP2), Pyruvate kinase (At2g36580), Pyruvate kinase (At3g04050), Pyruvate kinase (AXX17_At4g30430), Pyruvate kinase (F1I16_220), Plastidial pyruvate kinase 1, chloroplastic (PKP1), Pyruvate kinase (AXX17_At1g33230), Pyruvate kinase (At5g63680), Pyruvate kinase, Plastidial pyruvate kinase 4, chloroplastic (PKP4)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinaseUniRule annotationImported, Transferase
Biological processGlycolysisUniRule annotation
LigandMagnesiumUniRule annotation, PyruvateImported

Enzyme and pathway databases

BioCyciARA:AT5G56350-MONOMER.
ReactomeiR-ATH-6798695. Neutrophil degranulation.
R-ATH-70171. Glycolysis.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinaseUniRule annotation (EC:2.7.1.40UniRule annotation)
Gene namesi
Name:MCD7.8Imported
Synonyms:MCD7_8Imported
Ordered Locus Names:At5g56350Imported, At5g56350/MCD7.8Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G56350.
TAIRilocus:2161068. AT5G56350.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Expressioni

Gene expression databases

ExpressionAtlasiQ9FM97. baseline and differential.

Interactioni

Protein-protein interaction databases

IntActiQ9FM97. 2 interactors.
STRINGi3702.AT5G56350.1.

Structurei

3D structure databases

SMRiQ9FM97.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 351PKInterPro annotationAdd BLAST343
Domaini368 – 494PK_CInterPro annotationAdd BLAST127

Sequence similaritiesi

Belongs to the pyruvate kinase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2323. Eukaryota.
COG0469. LUCA.
HOGENOMiHOG000021559.
KOiK00873.
OMAiGSYEDHA.
OrthoDBiEOG093607JW.

Family and domain databases

CDDicd00288. Pyruvate_Kinase. 1 hit.
Gene3Di3.40.1380.20. 1 hit.
InterProiView protein in InterPro
IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom_sf.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR036918. Pyrv_Knase_C_sf.
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiView protein in Pfam
PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiView protein in PROSITE
PS00110. PYRUVATE_KINASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9FM97-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMIEQRPKT KIVCTLGPAS RSVPMVEKLL RAGMNVARFN FSHGSHEYHQ
60 70 80 90 100
ETLDNLHQAM LNTGILCAVM LDTKGPEIRT GFLKDGKPIQ LKQGQEITIS
110 120 130 140 150
TDYDLKGDEN TICMSYKKLA VDVNPGMVIL CADGTISLLV LSCDKENGTV
160 170 180 190 200
RCRCENSAML GERKNVNLPG VVVDLPTLTE KDKEDIMQWG VPNQIDMIAL
210 220 230 240 250
SFVRKGSDLV QVRKLLGKHA KNILLMSKVE NQEGVANFDD ILVNSDAFMI
260 270 280 290 300
ARGDLGMEIP IEKIFLAQKV MIYKCNIQGK PVVTATQMLE SMIKSPRPTR
310 320 330 340 350
AEATDVANAV LDGTDCVMLS GETAAGAYPE LAVRTMAKIC VEAESTLDYG
360 370 380 390 400
DVFKRIMLYS PVPMSPLESL ASSAVRTANS ARATLIMVLT RGGSTARLVA
410 420 430 440 450
KYRPGMPILS VVVPEIKTDF FDWSCSDESP ARHSLIFRGL IPVLYAGSAR
460 470 480 490
ASHDESTEEA IEFATQYGKE KELCKTGDSV VALLRVGNAS VIKILTVK
Length:498
Mass (Da):54,411
Last modified:March 1, 2001 - v1
Checksum:i90C1374AC09079B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY054551 mRNA. Translation: AAK96742.1.
AY064679 mRNA. Translation: AAL47384.1.
AY087091 mRNA. Translation: AAM64651.1.
CP002688 Genomic DNA. Translation: AED96752.1.
AB009049 Genomic DNA. Translation: BAB11262.1.
RefSeqiNP_200446.1. NM_125018.3.
UniGeneiAt.20243.
At.74779.

Genome annotation databases

EnsemblPlantsiAT5G56350.1; AT5G56350.1; AT5G56350.
GeneIDi835735.
GrameneiAT5G56350.1; AT5G56350.1; AT5G56350.
KEGGiath:AT5G56350.

Similar proteinsi

Entry informationi

Entry nameiQ9FM97_ARATH
AccessioniPrimary (citable) accession number: Q9FM97
Entry historyiIntegrated into UniProtKB/TrEMBL: March 1, 2001
Last sequence update: March 1, 2001
Last modified: December 20, 2017
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported