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Protein

Glucosidase 2 subunit beta

Gene

PSL4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Regulatory subunit of glucosidase II (By similarity). Essential for stable accumulation of the receptor EFR that determines the specific perception of bacterial elongation factor Tu (EF-Tu), a potent elicitor of the defense response to pathogen-associated molecular patterns (PAMPs). Required for sustained activation of EFR-mediated signaling, but not receptor FLS2-mediated signaling elicited by the bacterial flagellin flg22.By similarity1 Publication

Pathwayi

GO - Molecular functioni

  1. calmodulin binding Source: TAIR

GO - Biological processi

  1. defense response to bacterium Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Plant defense

Enzyme and pathway databases

ReactomeiREACT_190905. Advanced glycosylation endproduct receptor signaling.
REACT_190967. Calnexin/calreticulin cycle.
UniPathwayiUPA00957.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosidase 2 subunit beta
Alternative name(s):
Glucosidase II subunit beta
Protein PRIORITY IN SWEET LIFE 4
Gene namesi
Name:PSL4
Ordered Locus Names:At5g56360
ORF Names:MCD7.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G56360.

Subcellular locationi

Endoplasmic reticulum PROSITE-ProRule annotation

GO - Cellular componenti

  1. endoplasmic reticulum Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but mutant plants have compromised defense response induced by the bacterial elicitor elongation factor Tu (EF-Tu).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Sequence AnalysisAdd
BLAST
Chaini31 – 647617Glucosidase 2 subunit betaPRO_0000425976Add
BLAST

Expressioni

Tissue specificityi

Expressed in roots, rosette leaves, leaf blades, mature stems, cauline leaves, flower buds, flowers and siliques.1 Publication

Gene expression databases

GenevestigatoriQ9FM96.

Interactioni

Subunit structurei

Heterodimer of a catalytic alpha subunit (PSL5) and a beta subunit (PSL4).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9FM96.
SMRiQ9FM96. Positions 50-124.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili155 – 23581Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi644 – 6474Prevents secretion from ERPROSITE-ProRule annotation

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi198 – 353156Glu-rich (acidic)Add
BLAST
Compositional biasi374 – 44673Asp-richAdd
BLAST

Keywords - Domaini

Coiled coil, Signal

Phylogenomic databases

InParanoidiQ9FM96.
KOiK08288.
OMAiACPNGNF.
PhylomeDBiQ9FM96.

Family and domain databases

Gene3Di2.70.130.10. 1 hit.
InterProiIPR002172. LDrepeatLR_classA_rpt.
IPR009011. Man6P_isomerase_rcpt-bd_dom.
IPR028146. PRKCSH_N.
[Graphical view]
PfamiPF12999. PRKCSH-like. 1 hit.
[Graphical view]
SMARTiSM00192. LDLa. 1 hit.
[Graphical view]
SUPFAMiSSF50911. SSF50911. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FM96-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRVVVISSFV SVSLQLSFLL LLASAIRSSS SPPNDPFLGI SPQDEKYYKS
60 70 80 90 100
SSEIKCKDGS KKFTKAQLND DFCDCSDGTD EPGTSACPTG KFYCRNAGHS
110 120 130 140 150
PVILFSSRVN DGICDCCDGS DEYDGHVSCQ NTCWEAGKAA RENLKKKIET
160 170 180 190 200
YNQGLVIRRQ EIEQAKVGLE KDAAELKKLK SEQKILKGLV DQLKDRKEQI
210 220 230 240 250
EKVEEKERLQ KEKEEKEKKE AELAAQQGKG DAEEKTDDSE KVEESSHDEG
260 270 280 290 300
TPAVSQHDET THHDEIGNYK DYPSDEEPAA EGEPTSILDE ATHTNPADEH
310 320 330 340 350
VVERKEESTS SEDSSSPTDE SQNDGSAEKE ESDEVKKVED FVTEKKEELS
360 370 380 390 400
KEELGRLVAS RWTGEKSDKP TEADDIPKAD DQENHEHTPI TAHEADEDDG
410 420 430 440 450
FVSDGDEDTS DDGKYSDHEP EDDSYEEEYR HDSSSSYKSD ADDDVDFSET
460 470 480 490 500
TSNPTWLEKI QKTVKNILLA VNLFQTTPVD KSEADRVRKE YDESSSKLNK
510 520 530 540 550
IQSRISSLEK KLKQDFGPEK EFYSFHGRCF ESKQGKYTYK VCAYKEATQE
560 570 580 590 600
EGYSKTRLGE WDKFENSYQF MSYTNGEKCW NGPDRSLKVK LRCGLKNELM
610 620 630 640
DVDEPSRCEY AAILSTPARC LEDKLKELQQ KLEKLMNQDK PQNHDEL
Length:647
Mass (Da):73,214
Last modified:March 1, 2001 - v1
Checksum:i49CC4F6DF7026472
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti126 – 1261H → Q in AAM63282. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009049 Genomic DNA. Translation: BAB11263.1.
CP002688 Genomic DNA. Translation: AED96753.1.
AY086076 mRNA. Translation: AAM63282.1.
RefSeqiNP_568840.3. NM_125019.4.
UniGeneiAt.24963.

Genome annotation databases

EnsemblPlantsiAT5G56360.1; AT5G56360.1; AT5G56360.
GeneIDi835736.
KEGGiath:AT5G56360.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009049 Genomic DNA. Translation: BAB11263.1.
CP002688 Genomic DNA. Translation: AED96753.1.
AY086076 mRNA. Translation: AAM63282.1.
RefSeqiNP_568840.3. NM_125019.4.
UniGeneiAt.24963.

3D structure databases

ProteinModelPortaliQ9FM96.
SMRiQ9FM96. Positions 50-124.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G56360.1; AT5G56360.1; AT5G56360.
GeneIDi835736.
KEGGiath:AT5G56360.

Organism-specific databases

TAIRiAT5G56360.

Phylogenomic databases

InParanoidiQ9FM96.
KOiK08288.
OMAiACPNGNF.
PhylomeDBiQ9FM96.

Enzyme and pathway databases

UniPathwayiUPA00957.
ReactomeiREACT_190905. Advanced glycosylation endproduct receptor signaling.
REACT_190967. Calnexin/calreticulin cycle.

Miscellaneous databases

PROiQ9FM96.

Gene expression databases

GenevestigatoriQ9FM96.

Family and domain databases

Gene3Di2.70.130.10. 1 hit.
InterProiIPR002172. LDrepeatLR_classA_rpt.
IPR009011. Man6P_isomerase_rcpt-bd_dom.
IPR028146. PRKCSH_N.
[Graphical view]
PfamiPF12999. PRKCSH-like. 1 hit.
[Graphical view]
SMARTiSM00192. LDLa. 1 hit.
[Graphical view]
SUPFAMiSSF50911. SSF50911. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones."
    Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 5:41-54(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "The cellulose-deficient Arabidopsis mutant rsw3 is defective in a gene encoding a putative glucosidase II, an enzyme processing N-glycans during ER quality control."
    Burn J.E., Hurley U.A., Birch R.J., Arioli T., Cork A., Williamson R.E.
    Plant J. 32:949-960(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. "Uncoupling of sustained MAMP receptor signaling from early outputs in an Arabidopsis endoplasmic reticulum glucosidase II allele."
    Lu X., Tintor N., Mentzel T., Kombrink E., Boller T., Robatzek S., Schulze-Lefert P., Saijo Y.
    Proc. Natl. Acad. Sci. U.S.A. 106:22522-22527(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiPSL4_ARATH
AccessioniPrimary (citable) accession number: Q9FM96
Secondary accession number(s): Q8LDD0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: March 1, 2001
Last modified: January 7, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.