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Protein

Glucosidase 2 subunit beta

Gene

PSL4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Regulatory subunit of glucosidase II (By similarity). Essential for stable accumulation of the receptor EFR that determines the specific perception of bacterial elongation factor Tu (EF-Tu), a potent elicitor of the defense response to pathogen-associated molecular patterns (PAMPs). Required for sustained activation of EFR-mediated signaling, but not receptor FLS2-mediated signaling elicited by the bacterial flagellin flg22.By similarity1 Publication

Pathwayi: N-glycan metabolism

This protein is involved in the pathway N-glycan metabolism, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway N-glycan metabolism and in Glycan metabolism.

GO - Biological processi

  • defense response to bacterium Source: TAIR
  • N-glycan processing Source: InterPro

Keywordsi

Biological processPlant defense

Enzyme and pathway databases

UniPathwayiUPA00957

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosidase 2 subunit beta
Alternative name(s):
Glucosidase II subunit beta
Protein PRIORITY IN SWEET LIFE 4
Gene namesi
Name:PSL4
Ordered Locus Names:At5g56360
ORF Names:MCD7.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G56360
TAIRilocus:2161078 AT5G56360

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but mutant plants have compromised defense response induced by the bacterial elicitor elongation factor Tu (EF-Tu).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Sequence analysisAdd BLAST30
ChainiPRO_000042597631 – 647Glucosidase 2 subunit betaAdd BLAST617

Proteomic databases

PaxDbiQ9FM96
PRIDEiQ9FM96

PTM databases

iPTMnetiQ9FM96

Expressioni

Tissue specificityi

Expressed in roots, rosette leaves, leaf blades, mature stems, cauline leaves, flower buds, flowers and siliques.1 Publication

Gene expression databases

ExpressionAtlasiQ9FM96 baseline and differential
GenevisibleiQ9FM96 AT

Interactioni

Subunit structurei

Heterodimer of a catalytic alpha subunit (PSL5) and a beta subunit (PSL4).By similarity

Protein-protein interaction databases

BioGridi20980, 3 interactors
STRINGi3702.AT5G56360.1

Structurei

3D structure databases

ProteinModelPortaliQ9FM96
SMRiQ9FM96
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili155 – 235Sequence analysisAdd BLAST81

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi644 – 647Prevents secretion from ERPROSITE-ProRule annotation4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi198 – 353Glu-rich (acidic)Add BLAST156
Compositional biasi374 – 446Asp-richAdd BLAST73

Keywords - Domaini

Coiled coil, Signal

Phylogenomic databases

eggNOGiKOG2397 Eukaryota
ENOG410XPE3 LUCA
HOGENOMiHOG000237767
InParanoidiQ9FM96
KOiK08288
OMAiCYELTTN
OrthoDBiEOG093607YY
PhylomeDBiQ9FM96

Family and domain databases

Gene3Di2.70.130.10, 1 hit
InterProiView protein in InterPro
IPR026874 Glucosidase_2_bsu
IPR009011 Man6P_isomerase_rcpt-bd_dom_sf
IPR036607 PRKCSH
IPR028146 PRKCSH_N
PANTHERiPTHR12630:SF10 PTHR12630:SF10, 1 hit
PfamiView protein in Pfam
PF12999 PRKCSH-like, 1 hit
PF13015 PRKCSH_1, 1 hit
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FM96-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVVVISSFV SVSLQLSFLL LLASAIRSSS SPPNDPFLGI SPQDEKYYKS
60 70 80 90 100
SSEIKCKDGS KKFTKAQLND DFCDCSDGTD EPGTSACPTG KFYCRNAGHS
110 120 130 140 150
PVILFSSRVN DGICDCCDGS DEYDGHVSCQ NTCWEAGKAA RENLKKKIET
160 170 180 190 200
YNQGLVIRRQ EIEQAKVGLE KDAAELKKLK SEQKILKGLV DQLKDRKEQI
210 220 230 240 250
EKVEEKERLQ KEKEEKEKKE AELAAQQGKG DAEEKTDDSE KVEESSHDEG
260 270 280 290 300
TPAVSQHDET THHDEIGNYK DYPSDEEPAA EGEPTSILDE ATHTNPADEH
310 320 330 340 350
VVERKEESTS SEDSSSPTDE SQNDGSAEKE ESDEVKKVED FVTEKKEELS
360 370 380 390 400
KEELGRLVAS RWTGEKSDKP TEADDIPKAD DQENHEHTPI TAHEADEDDG
410 420 430 440 450
FVSDGDEDTS DDGKYSDHEP EDDSYEEEYR HDSSSSYKSD ADDDVDFSET
460 470 480 490 500
TSNPTWLEKI QKTVKNILLA VNLFQTTPVD KSEADRVRKE YDESSSKLNK
510 520 530 540 550
IQSRISSLEK KLKQDFGPEK EFYSFHGRCF ESKQGKYTYK VCAYKEATQE
560 570 580 590 600
EGYSKTRLGE WDKFENSYQF MSYTNGEKCW NGPDRSLKVK LRCGLKNELM
610 620 630 640
DVDEPSRCEY AAILSTPARC LEDKLKELQQ KLEKLMNQDK PQNHDEL
Length:647
Mass (Da):73,214
Last modified:March 1, 2001 - v1
Checksum:i49CC4F6DF7026472
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti126H → Q in AAM63282 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009049 Genomic DNA Translation: BAB11263.1
CP002688 Genomic DNA Translation: AED96753.1
AY086076 mRNA Translation: AAM63282.1
RefSeqiNP_568840.3, NM_125019.5
UniGeneiAt.24963

Genome annotation databases

EnsemblPlantsiAT5G56360.1; AT5G56360.1; AT5G56360
GeneIDi835736
GrameneiAT5G56360.1; AT5G56360.1; AT5G56360
KEGGiath:AT5G56360

Similar proteinsi

Entry informationi

Entry nameiPSL4_ARATH
AccessioniPrimary (citable) accession number: Q9FM96
Secondary accession number(s): Q8LDD0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: March 1, 2001
Last modified: April 25, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health