Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9FM96 (PSL4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucosidase 2 subunit beta
Alternative name(s):
Glucosidase II subunit beta
Protein PRIORITY IN SWEET LIFE 4
Gene names
Name:PSL4
Ordered Locus Names:At5g56360
ORF Names:MCD7.9
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length647 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Regulatory subunit of glucosidase II By similarity. Essential for stable accumulation of the receptor EFR that determines the specific perception of bacterial elongation factor Tu (EF-Tu), a potent elicitor of the defense response to pathogen-associated molecular patterns (PAMPs). Required for sustained activation of EFR-mediated signaling, but not receptor FLS2-mediated signaling elicited by the bacterial flagellin flg22. Ref.5

Pathway

Glycan metabolism; N-glycan metabolism.

Subunit structure

Heterodimer of a catalytic alpha subunit (PSL5) and a beta subunit (PSL4) By similarity.

Subcellular location

Endoplasmic reticulum By similarity.

Tissue specificity

Expressed in roots, rosette leaves, leaf blades, mature stems, cauline leaves, flower buds, flowers and siliques. Ref.4

Disruption phenotype

No visible phenotype under normal growth conditions, but mutant plants have compromised defense response induced by the bacterial elicitor elongation factor Tu (EF-Tu). Ref.5

Ontologies

Keywords
   Biological processPlant defense
   Cellular componentEndoplasmic reticulum
   DomainCoiled coil
Signal
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdefense response to bacterium

Inferred from mutant phenotype Ref.5. Source: TAIR

   Cellular_componentendoplasmic reticulum

Inferred from direct assay PubMed 22923678. Source: TAIR

   Molecular_functioncalmodulin binding

Inferred from physical interaction PubMed 11782485. Source: TAIR

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030 Potential
Chain31 – 647617Glucosidase 2 subunit beta
PRO_0000425976

Regions

Coiled coil155 – 23581 Potential
Motif644 – 6474Prevents secretion from ER Potential
Compositional bias198 – 353156Glu-rich (acidic)
Compositional bias374 – 44673Asp-rich

Experimental info

Sequence conflict1261H → Q in AAM63282. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9FM96 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 49CC4F6DF7026472

FASTA64773,214
        10         20         30         40         50         60 
MRVVVISSFV SVSLQLSFLL LLASAIRSSS SPPNDPFLGI SPQDEKYYKS SSEIKCKDGS 

        70         80         90        100        110        120 
KKFTKAQLND DFCDCSDGTD EPGTSACPTG KFYCRNAGHS PVILFSSRVN DGICDCCDGS 

       130        140        150        160        170        180 
DEYDGHVSCQ NTCWEAGKAA RENLKKKIET YNQGLVIRRQ EIEQAKVGLE KDAAELKKLK 

       190        200        210        220        230        240 
SEQKILKGLV DQLKDRKEQI EKVEEKERLQ KEKEEKEKKE AELAAQQGKG DAEEKTDDSE 

       250        260        270        280        290        300 
KVEESSHDEG TPAVSQHDET THHDEIGNYK DYPSDEEPAA EGEPTSILDE ATHTNPADEH 

       310        320        330        340        350        360 
VVERKEESTS SEDSSSPTDE SQNDGSAEKE ESDEVKKVED FVTEKKEELS KEELGRLVAS 

       370        380        390        400        410        420 
RWTGEKSDKP TEADDIPKAD DQENHEHTPI TAHEADEDDG FVSDGDEDTS DDGKYSDHEP 

       430        440        450        460        470        480 
EDDSYEEEYR HDSSSSYKSD ADDDVDFSET TSNPTWLEKI QKTVKNILLA VNLFQTTPVD 

       490        500        510        520        530        540 
KSEADRVRKE YDESSSKLNK IQSRISSLEK KLKQDFGPEK EFYSFHGRCF ESKQGKYTYK 

       550        560        570        580        590        600 
VCAYKEATQE EGYSKTRLGE WDKFENSYQF MSYTNGEKCW NGPDRSLKVK LRCGLKNELM 

       610        620        630        640 
DVDEPSRCEY AAILSTPARC LEDKLKELQQ KLEKLMNQDK PQNHDEL 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones."
Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S.
DNA Res. 5:41-54(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"The cellulose-deficient Arabidopsis mutant rsw3 is defective in a gene encoding a putative glucosidase II, an enzyme processing N-glycans during ER quality control."
Burn J.E., Hurley U.A., Birch R.J., Arioli T., Cork A., Williamson R.E.
Plant J. 32:949-960(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[5]"Uncoupling of sustained MAMP receptor signaling from early outputs in an Arabidopsis endoplasmic reticulum glucosidase II allele."
Lu X., Tintor N., Mentzel T., Kombrink E., Boller T., Robatzek S., Schulze-Lefert P., Saijo Y.
Proc. Natl. Acad. Sci. U.S.A. 106:22522-22527(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB009049 Genomic DNA. Translation: BAB11263.1.
CP002688 Genomic DNA. Translation: AED96753.1.
AY086076 mRNA. Translation: AAM63282.1.
RefSeqNP_568840.3. NM_125019.4.
UniGeneAt.24963.

3D structure databases

ProteinModelPortalQ9FM96.
SMRQ9FM96. Positions 50-125, 522-621.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G56360.1; AT5G56360.1; AT5G56360.
GeneID835736.
KEGGath:AT5G56360.

Organism-specific databases

TAIRAT5G56360.

Phylogenomic databases

InParanoidQ9FM96.
KOK08288.
OMAGQHCWNG.
PhylomeDBQ9FM96.

Enzyme and pathway databases

UniPathwayUPA00957.

Gene expression databases

GenevestigatorQ9FM96.

Family and domain databases

Gene3D2.70.130.10. 1 hit.
InterProIPR002172. LDrepeatLR_classA_rpt.
IPR009011. Man6P_isomerase_rcpt-bd_dom.
IPR028146. PRKCSH_N.
[Graphical view]
PfamPF12999. PRKCSH-like. 1 hit.
[Graphical view]
SMARTSM00192. LDLa. 1 hit.
[Graphical view]
SUPFAMSSF50911. SSF50911. 1 hit.
PROSITEPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ9FM96.

Entry information

Entry namePSL4_ARATH
AccessionPrimary (citable) accession number: Q9FM96
Secondary accession number(s): Q8LDD0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: March 1, 2001
Last modified: June 11, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names