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Q9FM96

- PSL4_ARATH

UniProt

Q9FM96 - PSL4_ARATH

Protein

Glucosidase 2 subunit beta

Gene

PSL4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Regulatory subunit of glucosidase II By similarity. Essential for stable accumulation of the receptor EFR that determines the specific perception of bacterial elongation factor Tu (EF-Tu), a potent elicitor of the defense response to pathogen-associated molecular patterns (PAMPs). Required for sustained activation of EFR-mediated signaling, but not receptor FLS2-mediated signaling elicited by the bacterial flagellin flg22.By similarity1 Publication

    Pathwayi

    GO - Molecular functioni

    1. calmodulin binding Source: TAIR

    GO - Biological processi

    1. defense response to bacterium Source: TAIR

    Keywords - Biological processi

    Plant defense

    Enzyme and pathway databases

    ReactomeiREACT_190905. Advanced glycosylation endproduct receptor signaling.
    UniPathwayiUPA00957.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucosidase 2 subunit beta
    Alternative name(s):
    Glucosidase II subunit beta
    Protein PRIORITY IN SWEET LIFE 4
    Gene namesi
    Name:PSL4
    Ordered Locus Names:At5g56360
    ORF Names:MCD7.9
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G56360.

    Subcellular locationi

    Endoplasmic reticulum PROSITE-ProRule annotation

    GO - Cellular componenti

    1. endoplasmic reticulum Source: TAIR

    Keywords - Cellular componenti

    Endoplasmic reticulum

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype under normal growth conditions, but mutant plants have compromised defense response induced by the bacterial elicitor elongation factor Tu (EF-Tu).1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3030Sequence AnalysisAdd
    BLAST
    Chaini31 – 647617Glucosidase 2 subunit betaPRO_0000425976Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed in roots, rosette leaves, leaf blades, mature stems, cauline leaves, flower buds, flowers and siliques.1 Publication

    Gene expression databases

    GenevestigatoriQ9FM96.

    Interactioni

    Subunit structurei

    Heterodimer of a catalytic alpha subunit (PSL5) and a beta subunit (PSL4).By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FM96.
    SMRiQ9FM96. Positions 50-125, 522-621.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili155 – 23581Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi644 – 6474Prevents secretion from ERPROSITE-ProRule annotation

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi198 – 353156Glu-rich (acidic)Add
    BLAST
    Compositional biasi374 – 44673Asp-richAdd
    BLAST

    Keywords - Domaini

    Coiled coil, Signal

    Phylogenomic databases

    InParanoidiQ9FM96.
    KOiK08288.
    OMAiGQHCWNG.
    PhylomeDBiQ9FM96.

    Family and domain databases

    Gene3Di2.70.130.10. 1 hit.
    InterProiIPR002172. LDrepeatLR_classA_rpt.
    IPR009011. Man6P_isomerase_rcpt-bd_dom.
    IPR028146. PRKCSH_N.
    [Graphical view]
    PfamiPF12999. PRKCSH-like. 1 hit.
    [Graphical view]
    SMARTiSM00192. LDLa. 1 hit.
    [Graphical view]
    SUPFAMiSSF50911. SSF50911. 1 hit.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9FM96-1 [UniParc]FASTAAdd to Basket

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    MRVVVISSFV SVSLQLSFLL LLASAIRSSS SPPNDPFLGI SPQDEKYYKS    50
    SSEIKCKDGS KKFTKAQLND DFCDCSDGTD EPGTSACPTG KFYCRNAGHS 100
    PVILFSSRVN DGICDCCDGS DEYDGHVSCQ NTCWEAGKAA RENLKKKIET 150
    YNQGLVIRRQ EIEQAKVGLE KDAAELKKLK SEQKILKGLV DQLKDRKEQI 200
    EKVEEKERLQ KEKEEKEKKE AELAAQQGKG DAEEKTDDSE KVEESSHDEG 250
    TPAVSQHDET THHDEIGNYK DYPSDEEPAA EGEPTSILDE ATHTNPADEH 300
    VVERKEESTS SEDSSSPTDE SQNDGSAEKE ESDEVKKVED FVTEKKEELS 350
    KEELGRLVAS RWTGEKSDKP TEADDIPKAD DQENHEHTPI TAHEADEDDG 400
    FVSDGDEDTS DDGKYSDHEP EDDSYEEEYR HDSSSSYKSD ADDDVDFSET 450
    TSNPTWLEKI QKTVKNILLA VNLFQTTPVD KSEADRVRKE YDESSSKLNK 500
    IQSRISSLEK KLKQDFGPEK EFYSFHGRCF ESKQGKYTYK VCAYKEATQE 550
    EGYSKTRLGE WDKFENSYQF MSYTNGEKCW NGPDRSLKVK LRCGLKNELM 600
    DVDEPSRCEY AAILSTPARC LEDKLKELQQ KLEKLMNQDK PQNHDEL 647
    Length:647
    Mass (Da):73,214
    Last modified:March 1, 2001 - v1
    Checksum:i49CC4F6DF7026472
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti126 – 1261H → Q in AAM63282. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB009049 Genomic DNA. Translation: BAB11263.1.
    CP002688 Genomic DNA. Translation: AED96753.1.
    AY086076 mRNA. Translation: AAM63282.1.
    RefSeqiNP_568840.3. NM_125019.4.
    UniGeneiAt.24963.

    Genome annotation databases

    EnsemblPlantsiAT5G56360.1; AT5G56360.1; AT5G56360.
    GeneIDi835736.
    KEGGiath:AT5G56360.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB009049 Genomic DNA. Translation: BAB11263.1 .
    CP002688 Genomic DNA. Translation: AED96753.1 .
    AY086076 mRNA. Translation: AAM63282.1 .
    RefSeqi NP_568840.3. NM_125019.4.
    UniGenei At.24963.

    3D structure databases

    ProteinModelPortali Q9FM96.
    SMRi Q9FM96. Positions 50-125, 522-621.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G56360.1 ; AT5G56360.1 ; AT5G56360 .
    GeneIDi 835736.
    KEGGi ath:AT5G56360.

    Organism-specific databases

    TAIRi AT5G56360.

    Phylogenomic databases

    InParanoidi Q9FM96.
    KOi K08288.
    OMAi GQHCWNG.
    PhylomeDBi Q9FM96.

    Enzyme and pathway databases

    UniPathwayi UPA00957 .
    Reactomei REACT_190905. Advanced glycosylation endproduct receptor signaling.

    Miscellaneous databases

    PROi Q9FM96.

    Gene expression databases

    Genevestigatori Q9FM96.

    Family and domain databases

    Gene3Di 2.70.130.10. 1 hit.
    InterProi IPR002172. LDrepeatLR_classA_rpt.
    IPR009011. Man6P_isomerase_rcpt-bd_dom.
    IPR028146. PRKCSH_N.
    [Graphical view ]
    Pfami PF12999. PRKCSH-like. 1 hit.
    [Graphical view ]
    SMARTi SM00192. LDLa. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50911. SSF50911. 1 hit.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones."
      Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S.
      DNA Res. 5:41-54(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "The cellulose-deficient Arabidopsis mutant rsw3 is defective in a gene encoding a putative glucosidase II, an enzyme processing N-glycans during ER quality control."
      Burn J.E., Hurley U.A., Birch R.J., Arioli T., Cork A., Williamson R.E.
      Plant J. 32:949-960(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    5. "Uncoupling of sustained MAMP receptor signaling from early outputs in an Arabidopsis endoplasmic reticulum glucosidase II allele."
      Lu X., Tintor N., Mentzel T., Kombrink E., Boller T., Robatzek S., Schulze-Lefert P., Saijo Y.
      Proc. Natl. Acad. Sci. U.S.A. 106:22522-22527(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiPSL4_ARATH
    AccessioniPrimary (citable) accession number: Q9FM96
    Secondary accession number(s): Q8LDD0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 16, 2014
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways

    External Data

    Dasty 3