Reviewed, UniProtKB/Swiss-Prot Q9FM66 (PEL21_ARATH)

Names and origin

Protein names

Recommended name:
    Putative pectate lyase 21
    EC=4.2.2.2

Gene names

Ordered Locus Names:	At5g55720
ORF Names:	MDF20.16

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion. Required for its activity By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Sequence similarities	Belongs to the polysaccharide lyase 1 family.

Ontologies

Keywords
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 392	371	Putative pectate lyase 21		PRO_0000024886
Sites
	Active site	269	1	Potential
	Metal binding	189	1	Calcium By similarity
	Metal binding	213	1	Calcium By similarity
	Metal binding	217	1	Calcium By similarity
Amino acid modifications
	Glycosylation	38	1	N-linked (GlcNAc...) Potential
	Glycosylation	220	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9FM66-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 305F94A437C30ECB	FASTA	392	43,682
	10 20 30 40 50 60 MSIVCTFFLF LLNTSFAFAF AIPKPPIVRR LSTTVTSNST ASSCSANGNP IDECWRCDEN 70 80 90 100 110 120 WKDNRKNLAD CAVGFGRDSI GGRAGEFYTV TDSGDDNPLN PTPGTLRYAA TQDQPLWIIF 130 140 150 160 170 180 DRDMVIQLKQ DLQVASYKTI DGRGNNVQIA YGPCLTLYKV SNIIINNLYI HDCVPVKRNA 190 200 210 220 230 240 LSSLGGYSDG DGISIFESRD IWIDHCTLEK CYDGLIDAVN GSTDITISNS YMLNHNEVML 250 260 270 280 290 300 LGHSDEYSGD RDMRVTIAFN YFGEGLVQRM PRCRHGYFHI VNNIYRDWKM YAIGGSANPT 310 320 330 340 350 360 IFSQGNVFIA SNNQFTKEVT KRESADGDEE WKEWNWKSEG DEMVNGAFFT PSGKEDSPSY 370 380 390 AKFSSMVARP ASLLKTTHPS VGVLSCEIDQ AC

References

[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones." Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S. DNA Res. 5:41-54(1998) [PubMed: 9628582] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB009050 Genomic DNA. Translation: BAB09239.1.
IPI	IPI00535153.
RefSeq	NP_200383.1.
UniGene	At.29429
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9FM66.
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Proteomic databases
PRIDE	Q9FM66.
Genome annotation databases
GeneID	835666.
GenomeReviews	Gene locus AT5G55720 in contig BA000015_GR.
KEGG	ath:AT5G55720.
NMPDR	fig|3702.1.peg.27501.
Organism-specific databases
TAIR	At5g55720.
Phylogenomic databases
OMA	CITIQNV.
Enzyme and pathway databases
BRENDA	4.2.2.2. 302.
Gene expression databases
ArrayExpress	Q9FM66.
Genevestigator	Q9FM66.
GermOnline	AT5G55720. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR018082. AmbAllergen. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
PRINTS	PR00807. AMBALLERGEN.
SMART	SM00656. Amb_all. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PEL21_ARATH

Accession

Primary (citable) accession number: Q9FM66

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)