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Q9FLW9

- PKP2_ARATH

UniProt

Q9FLW9 - PKP2_ARATH

Protein

Plastidial pyruvate kinase 2

Gene

PKP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Required for plastidial pyruvate kinase activity. Involved in seed oil accumulation, embryo development and seed storage compounds mobilization upon germination.3 Publications

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.1 Publication
    Potassium.1 Publication

    pH dependencei

    Optimum pH is 7.8-8.0.2 Publications

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei140 – 1401SubstrateBy similarity
    Metal bindingi142 – 1421PotassiumBy similarity
    Metal bindingi175 – 1751PotassiumBy similarity
    Metal bindingi176 – 1761Potassium; via carbonyl oxygenBy similarity
    Sitei325 – 3251Transition state stabilizerBy similarity
    Metal bindingi327 – 3271MagnesiumSequence Analysis
    Binding sitei350 – 3501Substrate; via amide nitrogenBy similarity
    Metal bindingi351 – 3511MagnesiumBy similarity
    Binding sitei351 – 3511Substrate; via amide nitrogenBy similarity
    Binding sitei383 – 3831SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: UniProtKB
    3. potassium ion binding Source: UniProtKB
    4. pyruvate kinase activity Source: TAIR

    GO - Biological processi

    1. fatty acid biosynthetic process Source: TAIR
    2. glycolytic process Source: UniProtKB-UniPathway
    3. lipid metabolic process Source: TAIR
    4. response to cadmium ion Source: TAIR
    5. seed development Source: TAIR
    6. seed maturation Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    SABIO-RKQ9FLW9.
    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Plastidial pyruvate kinase 2 (EC:2.7.1.40)
    Short name:
    PKp2
    Alternative name(s):
    Plastidial pyruvate kinase 1
    Short name:
    PKP1
    Pyruvate kinase III
    Pyruvate kinase isozyme B1, chloroplastic
    Short name:
    PKP-BETA1
    Short name:
    Plastidic pyruvate kinase beta subunit 1
    Gene namesi
    Name:PKP2
    Synonyms:PKP1
    Ordered Locus Names:At5g52920
    ORF Names:MXC20.15
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G52920.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast stroma Source: TAIR
    3. mitochondrion Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Mitochondrion, Plastid

    Pathology & Biotechi

    Disruption phenotypei

    Reduced plastidial pyruvate kinase activity and altered seed oil content leading to wrinkled seeds, retarded embryo elongation and reduced seed germination.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 6363ChloroplastSequence AnalysisAdd
    BLAST
    Chaini64 – 579516Plastidial pyruvate kinase 2PRO_0000416988Add
    BLAST

    Proteomic databases

    PRIDEiQ9FLW9.

    Expressioni

    Tissue specificityi

    Mostly expressed in seeds, and, to a lower extent, in roots, leaves (veins and trichomes), inflorescences, siliques, pollen (grains and tubes) and flowers (sepals and petals).2 Publications

    Developmental stagei

    In seeds, accumulates in endosperm and embryo. In torpedo-shaped embryos, restricted to the hypocotyl and in the outer parts of the young cotyledons. In later embryo stages, present in all tissues except root tips.1 Publication

    Inductioni

    Repressed by cadmium.1 Publication

    Gene expression databases

    GenevestigatoriQ9FLW9.

    Interactioni

    Subunit structurei

    Oligomer of alpha and beta subunits.1 Publication

    Protein-protein interaction databases

    STRINGi3702.AT5G52920.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FLW9.
    SMRiQ9FLW9. Positions 110-564.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0469.
    HOGENOMiHOG000021559.
    InParanoidiQ9FLW9.
    KOiK00873.
    OMAiADMEYAR.
    PhylomeDBiQ9FLW9.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9FLW9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAQVVATRSI QGSMLSPNGG SVSTRSEKLL KPASFAVKVL GNEAKRSGRV    50
    SVRSRRVVDT TVRSARVETE VIPVSPEDVP NREEQLERLL EMQQFGDTSV 100
    GMWSKPTVRR KTKIVCTVGP STNTREMIWK LAEAGMNVAR MNMSHGDHAS 150
    HKKVIDLVKE YNAQTKDNTI AIMLDTKGPE VRSGDLPQPI MLDPGQEFTF 200
    TIERGVSTPS CVSVNYDDFV NDVEAGDMLL VDGGMMSFMV KSKTKDSVKC 250
    EVVDGGELKS RRHLNVRGKS ATLPSITEKD WEDIKFGVEN KVDFYAVSFV 300
    KDAQVVHELK KYLQNSGADI HVIVKIESAD SIPNLHSIIT ASDGAMVARG 350
    DLGAELPIEE VPILQEEIIN LCRSMGKAVI VATNMLESMI VHPTPTRAEV 400
    SDIAIAVREG ADAVMLSGET AHGKFPLKAA GVMHTVALRT EATITSGEMP 450
    PNLGQAFKNH MSEMFAYHAT MMSNTLGTST VVFTRTGFMA ILLSHYRPSG 500
    TIYAFTNEKK IQQRLALYQG VCPIYMEFTD DAEETFANAL ATLLKQGMVK 550
    KGEEIAIVQS GTQPIWRSQS THNIQVRKV 579
    Length:579
    Mass (Da):63,522
    Last modified:March 1, 2001 - v1
    Checksum:i9A728605C826B4D3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti342 – 3421S → P in AAK82461. (PubMed:14593172)Curated
    Sequence conflicti342 – 3421S → P in AAM10281. (PubMed:14593172)Curated
    Sequence conflicti383 – 3831T → A in BAD93771. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB009055 Genomic DNA. Translation: BAB10440.1.
    CP002688 Genomic DNA. Translation: AED96278.1.
    AY048198 mRNA. Translation: AAK82461.1.
    AY091682 mRNA. Translation: AAM10281.1.
    AK220807 mRNA. Translation: BAD94078.1.
    AK220873 mRNA. Translation: BAD94256.1.
    AK221740 mRNA. Translation: BAD93771.1.
    AY084507 mRNA. Translation: AAM61075.1.
    RefSeqiNP_200104.1. NM_124670.2.
    UniGeneiAt.20836.

    Genome annotation databases

    EnsemblPlantsiAT5G52920.1; AT5G52920.1; AT5G52920.
    GeneIDi835369.
    KEGGiath:AT5G52920.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB009055 Genomic DNA. Translation: BAB10440.1 .
    CP002688 Genomic DNA. Translation: AED96278.1 .
    AY048198 mRNA. Translation: AAK82461.1 .
    AY091682 mRNA. Translation: AAM10281.1 .
    AK220807 mRNA. Translation: BAD94078.1 .
    AK220873 mRNA. Translation: BAD94256.1 .
    AK221740 mRNA. Translation: BAD93771.1 .
    AY084507 mRNA. Translation: AAM61075.1 .
    RefSeqi NP_200104.1. NM_124670.2.
    UniGenei At.20836.

    3D structure databases

    ProteinModelPortali Q9FLW9.
    SMRi Q9FLW9. Positions 110-564.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT5G52920.1-P.

    Proteomic databases

    PRIDEi Q9FLW9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G52920.1 ; AT5G52920.1 ; AT5G52920 .
    GeneIDi 835369.
    KEGGi ath:AT5G52920.

    Organism-specific databases

    TAIRi AT5G52920.

    Phylogenomic databases

    eggNOGi COG0469.
    HOGENOMi HOG000021559.
    InParanoidi Q9FLW9.
    KOi K00873.
    OMAi ADMEYAR.
    PhylomeDBi Q9FLW9.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .
    SABIO-RK Q9FLW9.

    Gene expression databases

    Genevestigatori Q9FLW9.

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones."
      Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S.
      DNA Res. 5:41-54(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "Enzymes of glycolysis are functionally associated with the mitochondrion in Arabidopsis cells."
      Giege P., Heazlewood J.L., Roessner-Tunali U., Millar A.H., Fernie A.R., Leaver C.J., Sweetlove L.J.
      Plant Cell 15:2140-2151(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. "The early responses of Arabidopsis thaliana cells to cadmium exposure explored by protein and metabolite profiling analyses."
      Sarry J.-E., Kuhn L., Ducruix C., Lafaye A., Junot C., Hugouvieux V., Jourdain A., Bastien O., Fievet J.B., Vailhen D., Amekraz B., Moulin C., Ezan E., Garin J., Bourguignon J.
      Proteomics 6:2180-2198(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY CADMIUM [LARGE SCALE ANALYSIS].
      Strain: cv. Columbia.
    9. "A heteromeric plastidic pyruvate kinase complex involved in seed oil biosynthesis in Arabidopsis."
      Andre C., Froehlich J.E., Moll M.R., Benning C.
      Plant Cell 19:2006-2022(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBUNIT, SUBCELLULAR LOCATION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, GENE FAMILY.
    10. "Function of plastidial pyruvate kinases in seeds of Arabidopsis thaliana."
      Baud S., Wuilleme S., Dubreucq B., de Almeida A., Vuagnat C., Lepiniec L., Miquel M., Rochat C.
      Plant J. 52:405-419(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, GENE FAMILY, NOMENCLATURE.
      Strain: cv. Columbia and cv. Wassilewskija.
    11. "Arabidopsis seedlings deficient in a plastidic pyruvate kinase are unable to utilize seed storage compounds for germination and establishment."
      Andre C., Benning C.
      Plant Physiol. 145:1670-1680(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
      Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
      PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    13. "AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins."
      Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N.
      Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    14. "Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering."
      Olinares P.D., Ponnala L., van Wijk K.J.
      Mol. Cell. Proteomics 9:1594-1615(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiPKP2_ARATH
    AccessioniPrimary (citable) accession number: Q9FLW9
    Secondary accession number(s): Q56XD5
    , Q56ZT8, Q570A3, Q94AG4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 18, 2012
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3