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Q9FLW9

- PKP2_ARATH

UniProt

Q9FLW9 - PKP2_ARATH

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Protein

Plastidial pyruvate kinase 2

Gene

PKP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for plastidial pyruvate kinase activity. Involved in seed oil accumulation, embryo development and seed storage compounds mobilization upon germination.3 Publications

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+1 Publication
  • K(+)1 Publication

pH dependencei

Optimum pH is 7.8-8.0.2 Publications

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei140 – 1401SubstrateBy similarity
Metal bindingi142 – 1421PotassiumBy similarity
Metal bindingi175 – 1751PotassiumBy similarity
Metal bindingi176 – 1761Potassium; via carbonyl oxygenBy similarity
Sitei325 – 3251Transition state stabilizerBy similarity
Metal bindingi327 – 3271MagnesiumSequence Analysis
Binding sitei350 – 3501Substrate; via amide nitrogenBy similarity
Metal bindingi351 – 3511MagnesiumBy similarity
Binding sitei351 – 3511Substrate; via amide nitrogenBy similarity
Binding sitei383 – 3831SubstrateBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: UniProtKB
  3. potassium ion binding Source: UniProtKB
  4. pyruvate kinase activity Source: TAIR

GO - Biological processi

  1. fatty acid biosynthetic process Source: TAIR
  2. glycolytic process Source: UniProtKB-UniPathway
  3. lipid metabolic process Source: TAIR
  4. response to cadmium ion Source: TAIR
  5. seed development Source: TAIR
  6. seed maturation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

ReactomeiREACT_239741. Regulation of gene expression in beta cells.
REACT_248260. Glycolysis.
REACT_260729. ChREBP activates metabolic gene expression.
SABIO-RKQ9FLW9.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Plastidial pyruvate kinase 2 (EC:2.7.1.40)
Short name:
PKp2
Alternative name(s):
Plastidial pyruvate kinase 1
Short name:
PKP1
Pyruvate kinase III
Pyruvate kinase isozyme B1, chloroplastic
Short name:
PKP-BETA1
Short name:
Plastidic pyruvate kinase beta subunit 1
Gene namesi
Name:PKP2
Synonyms:PKP1
Ordered Locus Names:At5g52920
ORF Names:MXC20.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G52920.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast stroma Source: TAIR
  3. mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

Pathology & Biotechi

Disruption phenotypei

Reduced plastidial pyruvate kinase activity and altered seed oil content leading to wrinkled seeds, retarded embryo elongation and reduced seed germination.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6363ChloroplastSequence AnalysisAdd
BLAST
Chaini64 – 579516Plastidial pyruvate kinase 2PRO_0000416988Add
BLAST

Proteomic databases

PRIDEiQ9FLW9.

Expressioni

Tissue specificityi

Mostly expressed in seeds, and, to a lower extent, in roots, leaves (veins and trichomes), inflorescences, siliques, pollen (grains and tubes) and flowers (sepals and petals).2 Publications

Developmental stagei

In seeds, accumulates in endosperm and embryo. In torpedo-shaped embryos, restricted to the hypocotyl and in the outer parts of the young cotyledons. In later embryo stages, present in all tissues except root tips.1 Publication

Inductioni

Repressed by cadmium.1 Publication

Gene expression databases

GenevestigatoriQ9FLW9.

Interactioni

Subunit structurei

Oligomer of alpha and beta subunits.1 Publication

Protein-protein interaction databases

STRINGi3702.AT5G52920.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9FLW9.
SMRiQ9FLW9. Positions 110-564.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0469.
HOGENOMiHOG000021559.
InParanoidiQ9FLW9.
KOiK00873.
OMAiADMEYAR.
PhylomeDBiQ9FLW9.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FLW9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAQVVATRSI QGSMLSPNGG SVSTRSEKLL KPASFAVKVL GNEAKRSGRV
60 70 80 90 100
SVRSRRVVDT TVRSARVETE VIPVSPEDVP NREEQLERLL EMQQFGDTSV
110 120 130 140 150
GMWSKPTVRR KTKIVCTVGP STNTREMIWK LAEAGMNVAR MNMSHGDHAS
160 170 180 190 200
HKKVIDLVKE YNAQTKDNTI AIMLDTKGPE VRSGDLPQPI MLDPGQEFTF
210 220 230 240 250
TIERGVSTPS CVSVNYDDFV NDVEAGDMLL VDGGMMSFMV KSKTKDSVKC
260 270 280 290 300
EVVDGGELKS RRHLNVRGKS ATLPSITEKD WEDIKFGVEN KVDFYAVSFV
310 320 330 340 350
KDAQVVHELK KYLQNSGADI HVIVKIESAD SIPNLHSIIT ASDGAMVARG
360 370 380 390 400
DLGAELPIEE VPILQEEIIN LCRSMGKAVI VATNMLESMI VHPTPTRAEV
410 420 430 440 450
SDIAIAVREG ADAVMLSGET AHGKFPLKAA GVMHTVALRT EATITSGEMP
460 470 480 490 500
PNLGQAFKNH MSEMFAYHAT MMSNTLGTST VVFTRTGFMA ILLSHYRPSG
510 520 530 540 550
TIYAFTNEKK IQQRLALYQG VCPIYMEFTD DAEETFANAL ATLLKQGMVK
560 570
KGEEIAIVQS GTQPIWRSQS THNIQVRKV
Length:579
Mass (Da):63,522
Last modified:March 1, 2001 - v1
Checksum:i9A728605C826B4D3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti342 – 3421S → P in AAK82461. (PubMed:14593172)Curated
Sequence conflicti342 – 3421S → P in AAM10281. (PubMed:14593172)Curated
Sequence conflicti383 – 3831T → A in BAD93771. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009055 Genomic DNA. Translation: BAB10440.1.
CP002688 Genomic DNA. Translation: AED96278.1.
AY048198 mRNA. Translation: AAK82461.1.
AY091682 mRNA. Translation: AAM10281.1.
AK220807 mRNA. Translation: BAD94078.1.
AK220873 mRNA. Translation: BAD94256.1.
AK221740 mRNA. Translation: BAD93771.1.
AY084507 mRNA. Translation: AAM61075.1.
RefSeqiNP_200104.1. NM_124670.2.
UniGeneiAt.20836.

Genome annotation databases

EnsemblPlantsiAT5G52920.1; AT5G52920.1; AT5G52920.
GeneIDi835369.
KEGGiath:AT5G52920.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009055 Genomic DNA. Translation: BAB10440.1 .
CP002688 Genomic DNA. Translation: AED96278.1 .
AY048198 mRNA. Translation: AAK82461.1 .
AY091682 mRNA. Translation: AAM10281.1 .
AK220807 mRNA. Translation: BAD94078.1 .
AK220873 mRNA. Translation: BAD94256.1 .
AK221740 mRNA. Translation: BAD93771.1 .
AY084507 mRNA. Translation: AAM61075.1 .
RefSeqi NP_200104.1. NM_124670.2.
UniGenei At.20836.

3D structure databases

ProteinModelPortali Q9FLW9.
SMRi Q9FLW9. Positions 110-564.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT5G52920.1-P.

Proteomic databases

PRIDEi Q9FLW9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G52920.1 ; AT5G52920.1 ; AT5G52920 .
GeneIDi 835369.
KEGGi ath:AT5G52920.

Organism-specific databases

TAIRi AT5G52920.

Phylogenomic databases

eggNOGi COG0469.
HOGENOMi HOG000021559.
InParanoidi Q9FLW9.
KOi K00873.
OMAi ADMEYAR.
PhylomeDBi Q9FLW9.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .
Reactomei REACT_239741. Regulation of gene expression in beta cells.
REACT_248260. Glycolysis.
REACT_260729. ChREBP activates metabolic gene expression.
SABIO-RK Q9FLW9.

Gene expression databases

Genevestigatori Q9FLW9.

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones."
    Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 5:41-54(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Enzymes of glycolysis are functionally associated with the mitochondrion in Arabidopsis cells."
    Giege P., Heazlewood J.L., Roessner-Tunali U., Millar A.H., Fernie A.R., Leaver C.J., Sweetlove L.J.
    Plant Cell 15:2140-2151(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. "The early responses of Arabidopsis thaliana cells to cadmium exposure explored by protein and metabolite profiling analyses."
    Sarry J.-E., Kuhn L., Ducruix C., Lafaye A., Junot C., Hugouvieux V., Jourdain A., Bastien O., Fievet J.B., Vailhen D., Amekraz B., Moulin C., Ezan E., Garin J., Bourguignon J.
    Proteomics 6:2180-2198(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY CADMIUM [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.
  9. "A heteromeric plastidic pyruvate kinase complex involved in seed oil biosynthesis in Arabidopsis."
    Andre C., Froehlich J.E., Moll M.R., Benning C.
    Plant Cell 19:2006-2022(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBUNIT, SUBCELLULAR LOCATION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, GENE FAMILY.
  10. "Function of plastidial pyruvate kinases in seeds of Arabidopsis thaliana."
    Baud S., Wuilleme S., Dubreucq B., de Almeida A., Vuagnat C., Lepiniec L., Miquel M., Rochat C.
    Plant J. 52:405-419(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, GENE FAMILY, NOMENCLATURE.
    Strain: cv. Columbia and cv. Wassilewskija.
  11. "Arabidopsis seedlings deficient in a plastidic pyruvate kinase are unable to utilize seed storage compounds for germination and establishment."
    Andre C., Benning C.
    Plant Physiol. 145:1670-1680(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
    Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
    PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  13. "AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins."
    Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N.
    Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  14. "Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering."
    Olinares P.D., Ponnala L., van Wijk K.J.
    Mol. Cell. Proteomics 9:1594-1615(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.

Entry informationi

Entry nameiPKP2_ARATH
AccessioniPrimary (citable) accession number: Q9FLW9
Secondary accession number(s): Q56XD5
, Q56ZT8, Q570A3, Q94AG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: March 1, 2001
Last modified: November 26, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3