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Q9FLW9 (PKP2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Plastidial pyruvate kinase 2

Short name=PKp2
EC=2.7.1.40
Alternative name(s):
Plastidial pyruvate kinase 1
Short name=PKP1
Pyruvate kinase III
Pyruvate kinase isozyme B1, chloroplastic
Short name=PKP-BETA1
Short name=Plastidic pyruvate kinase beta subunit 1
Gene names
Name:PKP2
Synonyms:PKP1
Ordered Locus Names:At5g52920
ORF Names:MXC20.15
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length579 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for plastidial pyruvate kinase activity. Involved in seed oil accumulation, embryo development and seed storage compounds mobilization upon germination. Ref.9 Ref.10 Ref.11

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium. Ref.9

Potassium. Ref.9

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Oligomer of alpha and beta subunits. Ref.9

Subcellular location

Plastidchloroplast stroma. Mitochondrion Ref.6 Ref.7 Ref.9 Ref.10 Ref.12 Ref.13 Ref.14.

Tissue specificity

Mostly expressed in seeds, and, to a lower extent, in roots, leaves (veins and trichomes), inflorescences, siliques, pollen (grains and tubes) and flowers (sepals and petals). Ref.9 Ref.10

Developmental stage

In seeds, accumulates in endosperm and embryo. In torpedo-shaped embryos, restricted to the hypocotyl and in the outer parts of the young cotyledons. In later embryo stages, present in all tissues except root tips. Ref.10

Induction

Repressed by cadmium.

Disruption phenotype

Reduced plastidial pyruvate kinase activity and altered seed oil content leading to wrinkled seeds, retarded embryo elongation and reduced seed germination. Ref.9 Ref.10

Sequence similarities

Belongs to the pyruvate kinase family.

Biophysicochemical properties

pH dependence:

Optimum pH is 7.8-8.0. Ref.9 Ref.10

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 6363Chloroplast Potential
Chain64 – 579516Plastidial pyruvate kinase 2
PRO_0000416988

Sites

Metal binding1421Potassium By similarity
Metal binding1751Potassium By similarity
Metal binding1761Potassium; via carbonyl oxygen By similarity
Metal binding3271Magnesium Potential
Metal binding3511Magnesium By similarity
Binding site1401Substrate By similarity
Binding site3501Substrate; via amide nitrogen By similarity
Binding site3511Substrate; via amide nitrogen By similarity
Binding site3831Substrate By similarity
Site3251Transition state stabilizer By similarity

Experimental info

Sequence conflict3421S → P in AAK82461. Ref.3
Sequence conflict3421S → P in AAM10281. Ref.3
Sequence conflict3831T → A in BAD93771. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q9FLW9 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 9A728605C826B4D3

FASTA57963,522
        10         20         30         40         50         60 
MAQVVATRSI QGSMLSPNGG SVSTRSEKLL KPASFAVKVL GNEAKRSGRV SVRSRRVVDT 

        70         80         90        100        110        120 
TVRSARVETE VIPVSPEDVP NREEQLERLL EMQQFGDTSV GMWSKPTVRR KTKIVCTVGP 

       130        140        150        160        170        180 
STNTREMIWK LAEAGMNVAR MNMSHGDHAS HKKVIDLVKE YNAQTKDNTI AIMLDTKGPE 

       190        200        210        220        230        240 
VRSGDLPQPI MLDPGQEFTF TIERGVSTPS CVSVNYDDFV NDVEAGDMLL VDGGMMSFMV 

       250        260        270        280        290        300 
KSKTKDSVKC EVVDGGELKS RRHLNVRGKS ATLPSITEKD WEDIKFGVEN KVDFYAVSFV 

       310        320        330        340        350        360 
KDAQVVHELK KYLQNSGADI HVIVKIESAD SIPNLHSIIT ASDGAMVARG DLGAELPIEE 

       370        380        390        400        410        420 
VPILQEEIIN LCRSMGKAVI VATNMLESMI VHPTPTRAEV SDIAIAVREG ADAVMLSGET 

       430        440        450        460        470        480 
AHGKFPLKAA GVMHTVALRT EATITSGEMP PNLGQAFKNH MSEMFAYHAT MMSNTLGTST 

       490        500        510        520        530        540 
VVFTRTGFMA ILLSHYRPSG TIYAFTNEKK IQQRLALYQG VCPIYMEFTD DAEETFANAL 

       550        560        570 
ATLLKQGMVK KGEEIAIVQS GTQPIWRSQS THNIQVRKV 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones."
Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S.
DNA Res. 5:41-54(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"Enzymes of glycolysis are functionally associated with the mitochondrion in Arabidopsis cells."
Giege P., Heazlewood J.L., Roessner-Tunali U., Millar A.H., Fernie A.R., Leaver C.J., Sweetlove L.J.
Plant Cell 15:2140-2151(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
[7]"The oligomeric stromal proteome of Arabidopsis thaliana chloroplasts."
Peltier J.-B., Cai Y., Sun Q., Zabrouskov V., Giacomelli L., Rudella A., Ytterberg A.J., Rutschow H., van Wijk K.J.
Mol. Cell. Proteomics 5:114-133(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"The early responses of Arabidopsis thaliana cells to cadmium exposure explored by protein and metabolite profiling analyses."
Sarry J.-E., Kuhn L., Ducruix C., Lafaye A., Junot C., Hugouvieux V., Jourdain A., Bastien O., Fievet J.B., Vailhen D., Amekraz B., Moulin C., Ezan E., Garin J., Bourguignon J.
Proteomics 6:2180-2198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY CADMIUM [LARGE SCALE ANALYSIS].
Strain: cv. Columbia.
[9]"A heteromeric plastidic pyruvate kinase complex involved in seed oil biosynthesis in Arabidopsis."
Andre C., Froehlich J.E., Moll M.R., Benning C.
Plant Cell 19:2006-2022(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBUNIT, SUBCELLULAR LOCATION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, GENE FAMILY.
[10]"Function of plastidial pyruvate kinases in seeds of Arabidopsis thaliana."
Baud S., Wuilleme S., Dubreucq B., de Almeida A., Vuagnat C., Lepiniec L., Miquel M., Rochat C.
Plant J. 52:405-419(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, GENE FAMILY, NOMENCLATURE.
Strain: cv. Columbia and cv. Wassilewskija.
[11]"Arabidopsis seedlings deficient in a plastidic pyruvate kinase are unable to utilize seed storage compounds for germination and establishment."
Andre C., Benning C.
Plant Physiol. 145:1670-1680(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[13]"AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins."
Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N.
Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[14]"Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering."
Olinares P.D., Ponnala L., van Wijk K.J.
Mol. Cell. Proteomics 9:1594-1615(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB009055 Genomic DNA. Translation: BAB10440.1.
CP002688 Genomic DNA. Translation: AED96278.1.
AY048198 mRNA. Translation: AAK82461.1.
AY091682 mRNA. Translation: AAM10281.1.
AK220807 mRNA. Translation: BAD94078.1.
AK220873 mRNA. Translation: BAD94256.1.
AK221740 mRNA. Translation: BAD93771.1.
AY084507 mRNA. Translation: AAM61075.1.
RefSeqNP_200104.1. NM_124670.2.
UniGeneAt.20836.

3D structure databases

ProteinModelPortalQ9FLW9.
SMRQ9FLW9. Positions 110-564.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT5G52920.1-P.

Proteomic databases

PRIDEQ9FLW9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G52920.1; AT5G52920.1; AT5G52920.
GeneID835369.
KEGGath:AT5G52920.

Organism-specific databases

TAIRAT5G52920.

Phylogenomic databases

eggNOGCOG0469.
HOGENOMHOG000021559.
InParanoidQ9FLW9.
KOK00873.
OMAADMEYAR.
PhylomeDBQ9FLW9.

Enzyme and pathway databases

SABIO-RKQ9FLW9.
UniPathwayUPA00109; UER00188.

Gene expression databases

GenevestigatorQ9FLW9.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePKP2_ARATH
AccessionPrimary (citable) accession number: Q9FLW9
Secondary accession number(s): Q56XD5 expand/collapse secondary AC list , Q56ZT8, Q570A3, Q94AG4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: March 1, 2001
Last modified: June 11, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names