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Protein

Plastidial pyruvate kinase 2

Gene

PKP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for plastidial pyruvate kinase activity. Involved in seed oil accumulation, embryo development and seed storage compounds mobilization upon germination.3 Publications

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+1 Publication
  • K+1 Publication

pH dependencei

Optimum pH is 7.8-8.0.2 Publications

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase GAPC2, cytosolic (GAPC2), Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic (GAPC1)
  2. Phosphoglycerate kinase 3, cytosolic (PGK3)
  3. Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2 (At3g08590), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 (PGM1)
  4. Enolase 1, chloroplastic (ENO1), Cytosolic enolase 3 (ENO3), Bifunctional enolase 2/transcriptional activator (ENO2)
  5. Pyruvate kinase (AXX17_At5g51800), Pyruvate kinase (AXX17_At3g24740), Pyruvate kinase (AXX17_At3g50280), Pyruvate kinase (AXX17_ATUG04870), Pyruvate kinase (At5g08570), Pyruvate kinase (At5g56350), Pyruvate kinase (At3g25960), Pyruvate kinase (At5g63680), Pyruvate kinase (F1I16_60), Pyruvate kinase (AXX17_At3g50440), Plastidial pyruvate kinase 3, chloroplastic (PKP3), Pyruvate kinase (At3g52990), Pyruvate kinase (AXX17_At3g28070), Pyruvate kinase (At2g36580), Pyruvate kinase (AXX17_At3g47400), Pyruvate kinase, Probable pyruvate kinase, cytosolic isozyme (At4g26390), Pyruvate kinase (F8J2_160), Pyruvate kinase (At3g52990), Pyruvate kinase (AXX17_At3g03460), Plastidial pyruvate kinase 2 (PKP2), Pyruvate kinase (T11I18.16), Pyruvate kinase (AXX17_At4g30430), Pyruvate kinase (F1I16_220), Plastidial pyruvate kinase 1, chloroplastic (PKP1), Pyruvate kinase (AXX17_At1g33230), Pyruvate kinase (At5g63680), Pyruvate kinase, Plastidial pyruvate kinase 4, chloroplastic (PKP4)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei140SubstrateBy similarity1
Metal bindingi142PotassiumBy similarity1
Metal bindingi144PotassiumBy similarity1
Metal bindingi175PotassiumBy similarity1
Metal bindingi176Potassium; via carbonyl oxygenBy similarity1
Binding sitei325Substrate; via amide nitrogenBy similarity1
Sitei325Transition state stabilizerBy similarity1
Metal bindingi327MagnesiumBy similarity1
Binding sitei350Substrate; via amide nitrogenBy similarity1
Metal bindingi351MagnesiumBy similarity1
Binding sitei351Substrate; via amide nitrogenBy similarity1
Binding sitei383SubstrateBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: UniProtKB-KW
  • magnesium ion binding Source: UniProtKB
  • potassium ion binding Source: UniProtKB
  • pyruvate kinase activity Source: TAIR

GO - Biological processi

  • fatty acid biosynthetic process Source: TAIR
  • lipid metabolic process Source: TAIR
  • response to cadmium ion Source: TAIR
  • seed development Source: TAIR
  • seed maturation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

SABIO-RKQ9FLW9.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Plastidial pyruvate kinase 2 (EC:2.7.1.40)
Short name:
PKp2
Alternative name(s):
Plastidial pyruvate kinase 1
Short name:
PKP1
Pyruvate kinase III
Pyruvate kinase isozyme B1, chloroplastic
Short name:
PKP-BETA1
Short name:
Plastidic pyruvate kinase beta subunit 1
Gene namesi
Name:PKP2
Synonyms:PKP1
Ordered Locus Names:At5g52920
ORF Names:MXC20.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G52920.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

Pathology & Biotechi

Disruption phenotypei

Reduced plastidial pyruvate kinase activity and altered seed oil content leading to wrinkled seeds, retarded embryo elongation and reduced seed germination.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 63ChloroplastSequence analysisAdd BLAST63
ChainiPRO_000041698864 – 579Plastidial pyruvate kinase 2Add BLAST516

Proteomic databases

PaxDbiQ9FLW9.
PRIDEiQ9FLW9.

PTM databases

iPTMnetiQ9FLW9.

Expressioni

Tissue specificityi

Mostly expressed in seeds, and, to a lower extent, in roots, leaves (veins and trichomes), inflorescences, siliques, pollen (grains and tubes) and flowers (sepals and petals).2 Publications

Developmental stagei

In seeds, accumulates in endosperm and embryo. In torpedo-shaped embryos, restricted to the hypocotyl and in the outer parts of the young cotyledons. In later embryo stages, present in all tissues except root tips.1 Publication

Inductioni

Repressed by cadmium.1 Publication

Gene expression databases

GenevisibleiQ9FLW9. AT.

Interactioni

Subunit structurei

Oligomer of alpha and beta subunits.1 Publication

Protein-protein interaction databases

STRINGi3702.AT5G52920.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FLW9.
SMRiQ9FLW9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2323. Eukaryota.
COG0469. LUCA.
HOGENOMiHOG000021559.
InParanoidiQ9FLW9.
KOiK00873.
OMAiEKMPSVN.
OrthoDBiEOG09360578.
PhylomeDBiQ9FLW9.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FLW9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQVVATRSI QGSMLSPNGG SVSTRSEKLL KPASFAVKVL GNEAKRSGRV
60 70 80 90 100
SVRSRRVVDT TVRSARVETE VIPVSPEDVP NREEQLERLL EMQQFGDTSV
110 120 130 140 150
GMWSKPTVRR KTKIVCTVGP STNTREMIWK LAEAGMNVAR MNMSHGDHAS
160 170 180 190 200
HKKVIDLVKE YNAQTKDNTI AIMLDTKGPE VRSGDLPQPI MLDPGQEFTF
210 220 230 240 250
TIERGVSTPS CVSVNYDDFV NDVEAGDMLL VDGGMMSFMV KSKTKDSVKC
260 270 280 290 300
EVVDGGELKS RRHLNVRGKS ATLPSITEKD WEDIKFGVEN KVDFYAVSFV
310 320 330 340 350
KDAQVVHELK KYLQNSGADI HVIVKIESAD SIPNLHSIIT ASDGAMVARG
360 370 380 390 400
DLGAELPIEE VPILQEEIIN LCRSMGKAVI VATNMLESMI VHPTPTRAEV
410 420 430 440 450
SDIAIAVREG ADAVMLSGET AHGKFPLKAA GVMHTVALRT EATITSGEMP
460 470 480 490 500
PNLGQAFKNH MSEMFAYHAT MMSNTLGTST VVFTRTGFMA ILLSHYRPSG
510 520 530 540 550
TIYAFTNEKK IQQRLALYQG VCPIYMEFTD DAEETFANAL ATLLKQGMVK
560 570
KGEEIAIVQS GTQPIWRSQS THNIQVRKV
Length:579
Mass (Da):63,522
Last modified:March 1, 2001 - v1
Checksum:i9A728605C826B4D3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti342S → P in AAK82461 (PubMed:14593172).Curated1
Sequence conflicti342S → P in AAM10281 (PubMed:14593172).Curated1
Sequence conflicti383T → A in BAD93771 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009055 Genomic DNA. Translation: BAB10440.1.
CP002688 Genomic DNA. Translation: AED96278.1.
AY048198 mRNA. Translation: AAK82461.1.
AY091682 mRNA. Translation: AAM10281.1.
AK220807 mRNA. Translation: BAD94078.1.
AK220873 mRNA. Translation: BAD94256.1.
AK221740 mRNA. Translation: BAD93771.1.
AY084507 mRNA. Translation: AAM61075.1.
RefSeqiNP_200104.1. NM_124670.3.
UniGeneiAt.20836.

Genome annotation databases

EnsemblPlantsiAT5G52920.1; AT5G52920.1; AT5G52920.
GeneIDi835369.
GrameneiAT5G52920.1; AT5G52920.1; AT5G52920.
KEGGiath:AT5G52920.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009055 Genomic DNA. Translation: BAB10440.1.
CP002688 Genomic DNA. Translation: AED96278.1.
AY048198 mRNA. Translation: AAK82461.1.
AY091682 mRNA. Translation: AAM10281.1.
AK220807 mRNA. Translation: BAD94078.1.
AK220873 mRNA. Translation: BAD94256.1.
AK221740 mRNA. Translation: BAD93771.1.
AY084507 mRNA. Translation: AAM61075.1.
RefSeqiNP_200104.1. NM_124670.3.
UniGeneiAt.20836.

3D structure databases

ProteinModelPortaliQ9FLW9.
SMRiQ9FLW9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G52920.1.

PTM databases

iPTMnetiQ9FLW9.

Proteomic databases

PaxDbiQ9FLW9.
PRIDEiQ9FLW9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G52920.1; AT5G52920.1; AT5G52920.
GeneIDi835369.
GrameneiAT5G52920.1; AT5G52920.1; AT5G52920.
KEGGiath:AT5G52920.

Organism-specific databases

TAIRiAT5G52920.

Phylogenomic databases

eggNOGiKOG2323. Eukaryota.
COG0469. LUCA.
HOGENOMiHOG000021559.
InParanoidiQ9FLW9.
KOiK00873.
OMAiEKMPSVN.
OrthoDBiEOG09360578.
PhylomeDBiQ9FLW9.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.
SABIO-RKQ9FLW9.

Miscellaneous databases

PROiQ9FLW9.

Gene expression databases

GenevisibleiQ9FLW9. AT.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPKP2_ARATH
AccessioniPrimary (citable) accession number: Q9FLW9
Secondary accession number(s): Q56XD5
, Q56ZT8, Q570A3, Q94AG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.