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Q9FLI3

- P2C75_ARATH

UniProt

Q9FLI3 - P2C75_ARATH

Protein

Probable protein phosphatase 2C 75

Gene

AHG1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Negative regulator of abscisic acid (ABA) responses during seed germination.1 Publication

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi149 – 1491Manganese 1By similarity
    Metal bindingi149 – 1491Manganese 2By similarity
    Metal bindingi150 – 1501Manganese 1; via carbonyl oxygenBy similarity
    Metal bindingi337 – 3371Manganese 2By similarity
    Metal bindingi402 – 4021Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein serine/threonine phosphatase activity Source: InterPro

    GO - Biological processi

    1. protein dephosphorylation Source: InterPro
    2. response to abscisic acid Source: TAIR

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G51760-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable protein phosphatase 2C 75 (EC:3.1.3.16)
    Short name:
    AtPP2C75
    Alternative name(s):
    Protein ABA-HYPERSENSITIVE GERMINATION 1
    Protein phosphatase 2C AHG1
    Short name:
    PP2C AHG1
    Gene namesi
    Name:AHG1
    Ordered Locus Names:At5g51760
    ORF Names:MIO24.11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G51760.

    Pathology & Biotechi

    Disruption phenotypei

    Hypersensitivity to ABA, salt and sucrose during seed germination.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 416416Probable protein phosphatase 2C 75PRO_0000367995Add
    BLAST

    Proteomic databases

    PaxDbiQ9FLI3.
    PRIDEiQ9FLI3.

    Expressioni

    Developmental stagei

    During germination, expressed in the embryo, vascular bundles and the root cap. In 6-day-old seedlings, expression in seed coat/endosperm disappears is detected only at the bases of the lateral root buds. Expressed in developing an mature siliques from 10 to 16 days after flowering (DAF).1 Publication

    Inductioni

    By ABA.1 Publication

    Gene expression databases

    GenevestigatoriQ9FLI3.

    Interactioni

    Protein-protein interaction databases

    BioGridi20495. 6 interactions.
    DIPiDIP-48990N.
    IntActiQ9FLI3. 1 interaction.
    STRINGi3702.AT5G51760.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FLI3.
    SMRiQ9FLI3. Positions 82-413.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini107 – 404298PP2C-likeAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi22 – 287Poly-Arg

    Sequence similaritiesi

    Belongs to the PP2C family.Curated
    Contains 1 PP2C-like domain.Curated

    Phylogenomic databases

    eggNOGiCOG0631.
    HOGENOMiHOG000233896.
    InParanoidiQ9FLI3.
    KOiK14497.
    OMAiYRTISTG.
    PhylomeDBiQ9FLI3.

    Family and domain databases

    Gene3Di3.60.40.10. 1 hit.
    InterProiIPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view]
    PANTHERiPTHR13832. PTHR13832. 1 hit.
    PfamiPF00481. PP2C. 1 hit.
    [Graphical view]
    SMARTiSM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF81606. SSF81606. 1 hit.
    PROSITEiPS01032. PP2C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9FLI3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTEIYRTIST GRGDDVSPTK CRERRRRRIE MRRQAAVFGE PSSSRNRDRT    50
    DMEVYSSFDV PLRKQARRSE IGGLPADIGG FLAPPAASSC QKSEAPVWKG 100
    EETEDEPLYG IVSVMGRSRK MEDSVTVKPN LCKPEVNRQR PVHFFAVYDG 150
    HGGSQVSTLC STTMHTFVKE ELEQNLEEEE EGSENDVVER KWRGVMKRSF 200
    KRMDEMATST CVCGTSVPLC NCDPREAAIS GSTAVTAVLT HDHIIVANTG 250
    DSRAVLCRNG MAIPLSNDHK PDRPDERARI EAAGGRVLVV DGARVEGILA 300
    TSRAIGDRYL KPMVAWEPEV TFMRRESGDE CLVLASDGLW DVLSSQLACD 350
    IARFCLREET PSSLDLNRMA QEDDNDGEQN PSRSVLAATL LTRLALGRQS 400
    SDNISVVVID LKNSSQ 416
    Length:416
    Mass (Da):46,043
    Last modified:March 1, 2001 - v1
    Checksum:i7272F86C929FF020
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB010074 Genomic DNA. Translation: BAB11245.1.
    CP002688 Genomic DNA. Translation: AED96123.1.
    RefSeqiNP_199989.1. NM_124555.2.
    UniGeneiAt.43187.

    Genome annotation databases

    EnsemblPlantsiAT5G51760.1; AT5G51760.1; AT5G51760.
    GeneIDi835250.
    KEGGiath:AT5G51760.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB010074 Genomic DNA. Translation: BAB11245.1 .
    CP002688 Genomic DNA. Translation: AED96123.1 .
    RefSeqi NP_199989.1. NM_124555.2.
    UniGenei At.43187.

    3D structure databases

    ProteinModelPortali Q9FLI3.
    SMRi Q9FLI3. Positions 82-413.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 20495. 6 interactions.
    DIPi DIP-48990N.
    IntActi Q9FLI3. 1 interaction.
    STRINGi 3702.AT5G51760.1-P.

    Proteomic databases

    PaxDbi Q9FLI3.
    PRIDEi Q9FLI3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G51760.1 ; AT5G51760.1 ; AT5G51760 .
    GeneIDi 835250.
    KEGGi ath:AT5G51760.

    Organism-specific databases

    TAIRi AT5G51760.

    Phylogenomic databases

    eggNOGi COG0631.
    HOGENOMi HOG000233896.
    InParanoidi Q9FLI3.
    KOi K14497.
    OMAi YRTISTG.
    PhylomeDBi Q9FLI3.

    Enzyme and pathway databases

    BioCyci ARA:AT5G51760-MONOMER.

    Gene expression databases

    Genevestigatori Q9FLI3.

    Family and domain databases

    Gene3Di 3.60.40.10. 1 hit.
    InterProi IPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view ]
    PANTHERi PTHR13832. PTHR13832. 1 hit.
    Pfami PF00481. PP2C. 1 hit.
    [Graphical view ]
    SMARTi SM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81606. SSF81606. 1 hit.
    PROSITEi PS01032. PP2C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones."
      Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S.
      DNA Res. 5:41-54(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "ABA-Hypersensitive Germination1 encodes a protein phosphatase 2C, an essential component of abscisic acid signaling in Arabidopsis seed."
      Nishimura N., Yoshida T., Kitahata N., Asami T., Shinozaki K., Hirayama T.
      Plant J. 50:935-949(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DEVELOPMENTAL STAGE, INDUCTION BY ABA, DISRUPTION PHENOTYPE.
    4. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
      Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
      BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiP2C75_ARATH
    AccessioniPrimary (citable) accession number: Q9FLI3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3