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Protein

Probable protein phosphatase 2C 75

Gene

AHG1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Negative regulator of abscisic acid (ABA) responses during seed germination.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi149 – 1491Manganese 1By similarity
Metal bindingi149 – 1491Manganese 2By similarity
Metal bindingi150 – 1501Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi337 – 3371Manganese 2By similarity
Metal bindingi402 – 4021Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein serine/threonine phosphatase activity Source: InterPro

GO - Biological processi

  1. protein dephosphorylation Source: InterPro
  2. response to abscisic acid Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G51760-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protein phosphatase 2C 75 (EC:3.1.3.16)
Short name:
AtPP2C75
Alternative name(s):
Protein ABA-HYPERSENSITIVE GERMINATION 1
Protein phosphatase 2C AHG1
Short name:
PP2C AHG1
Gene namesi
Name:AHG1
Ordered Locus Names:At5g51760
ORF Names:MIO24.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G51760.

Pathology & Biotechi

Disruption phenotypei

Hypersensitivity to ABA, salt and sucrose during seed germination.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 416416Probable protein phosphatase 2C 75PRO_0000367995Add
BLAST

Proteomic databases

PaxDbiQ9FLI3.
PRIDEiQ9FLI3.

Expressioni

Developmental stagei

During germination, expressed in the embryo, vascular bundles and the root cap. In 6-day-old seedlings, expression in seed coat/endosperm disappears is detected only at the bases of the lateral root buds. Expressed in developing an mature siliques from 10 to 16 days after flowering (DAF).1 Publication

Inductioni

By ABA.1 Publication

Gene expression databases

GenevestigatoriQ9FLI3.

Interactioni

Protein-protein interaction databases

BioGridi20495. 6 interactions.
DIPiDIP-48990N.
IntActiQ9FLI3. 1 interaction.
STRINGi3702.AT5G51760.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9FLI3.
SMRiQ9FLI3. Positions 82-413.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini107 – 404298PP2C-likeAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi22 – 287Poly-Arg

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PP2C-like domain.Curated

Phylogenomic databases

eggNOGiCOG0631.
HOGENOMiHOG000233896.
InParanoidiQ9FLI3.
KOiK14497.
OMAiYRTISTG.
PhylomeDBiQ9FLI3.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FLI3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTEIYRTIST GRGDDVSPTK CRERRRRRIE MRRQAAVFGE PSSSRNRDRT
60 70 80 90 100
DMEVYSSFDV PLRKQARRSE IGGLPADIGG FLAPPAASSC QKSEAPVWKG
110 120 130 140 150
EETEDEPLYG IVSVMGRSRK MEDSVTVKPN LCKPEVNRQR PVHFFAVYDG
160 170 180 190 200
HGGSQVSTLC STTMHTFVKE ELEQNLEEEE EGSENDVVER KWRGVMKRSF
210 220 230 240 250
KRMDEMATST CVCGTSVPLC NCDPREAAIS GSTAVTAVLT HDHIIVANTG
260 270 280 290 300
DSRAVLCRNG MAIPLSNDHK PDRPDERARI EAAGGRVLVV DGARVEGILA
310 320 330 340 350
TSRAIGDRYL KPMVAWEPEV TFMRRESGDE CLVLASDGLW DVLSSQLACD
360 370 380 390 400
IARFCLREET PSSLDLNRMA QEDDNDGEQN PSRSVLAATL LTRLALGRQS
410
SDNISVVVID LKNSSQ
Length:416
Mass (Da):46,043
Last modified:March 1, 2001 - v1
Checksum:i7272F86C929FF020
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010074 Genomic DNA. Translation: BAB11245.1.
CP002688 Genomic DNA. Translation: AED96123.1.
RefSeqiNP_199989.1. NM_124555.2.
UniGeneiAt.43187.

Genome annotation databases

EnsemblPlantsiAT5G51760.1; AT5G51760.1; AT5G51760.
GeneIDi835250.
KEGGiath:AT5G51760.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010074 Genomic DNA. Translation: BAB11245.1.
CP002688 Genomic DNA. Translation: AED96123.1.
RefSeqiNP_199989.1. NM_124555.2.
UniGeneiAt.43187.

3D structure databases

ProteinModelPortaliQ9FLI3.
SMRiQ9FLI3. Positions 82-413.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi20495. 6 interactions.
DIPiDIP-48990N.
IntActiQ9FLI3. 1 interaction.
STRINGi3702.AT5G51760.1-P.

Proteomic databases

PaxDbiQ9FLI3.
PRIDEiQ9FLI3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G51760.1; AT5G51760.1; AT5G51760.
GeneIDi835250.
KEGGiath:AT5G51760.

Organism-specific databases

TAIRiAT5G51760.

Phylogenomic databases

eggNOGiCOG0631.
HOGENOMiHOG000233896.
InParanoidiQ9FLI3.
KOiK14497.
OMAiYRTISTG.
PhylomeDBiQ9FLI3.

Enzyme and pathway databases

BioCyciARA:AT5G51760-MONOMER.

Gene expression databases

GenevestigatoriQ9FLI3.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones."
    Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 5:41-54(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "ABA-Hypersensitive Germination1 encodes a protein phosphatase 2C, an essential component of abscisic acid signaling in Arabidopsis seed."
    Nishimura N., Yoshida T., Kitahata N., Asami T., Shinozaki K., Hirayama T.
    Plant J. 50:935-949(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE, INDUCTION BY ABA, DISRUPTION PHENOTYPE.
  4. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
    Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
    BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiP2C75_ARATH
AccessioniPrimary (citable) accession number: Q9FLI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: March 1, 2001
Last modified: January 7, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.