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Q9FLI3 (P2C75_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable protein phosphatase 2C 75

Short name=AtPP2C75
EC=3.1.3.16
Alternative name(s):
Protein ABA-HYPERSENSITIVE GERMINATION 1
Protein phosphatase 2C AHG1
Short name=PP2C AHG1
Gene names
Name:AHG1
Ordered Locus Names:At5g51760
ORF Names:MIO24.11
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length416 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Negative regulator of abscisic acid (ABA) responses during seed germination. Ref.3

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Developmental stage

During germination, expressed in the embryo, vascular bundles and the root cap. In 6-day-old seedlings, expression in seed coat/endosperm disappears is detected only at the bases of the lateral root buds. Expressed in developing an mature siliques from 10 to 16 days after flowering (DAF). Ref.3

Induction

By ABA. Ref.3

Disruption phenotype

Hypersensitivity to ABA, salt and sucrose during seed germination. Ref.3

Sequence similarities

Belongs to the PP2C family.

Contains 1 PP2C-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 416416Probable protein phosphatase 2C 75
PRO_0000367995

Regions

Domain107 – 404298PP2C-like
Compositional bias22 – 287Poly-Arg

Sites

Metal binding1491Manganese 1 By similarity
Metal binding1491Manganese 2 By similarity
Metal binding1501Manganese 1; via carbonyl oxygen By similarity
Metal binding3371Manganese 2 By similarity
Metal binding4021Manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9FLI3 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 7272F86C929FF020

FASTA41646,043
        10         20         30         40         50         60 
MTEIYRTIST GRGDDVSPTK CRERRRRRIE MRRQAAVFGE PSSSRNRDRT DMEVYSSFDV 

        70         80         90        100        110        120 
PLRKQARRSE IGGLPADIGG FLAPPAASSC QKSEAPVWKG EETEDEPLYG IVSVMGRSRK 

       130        140        150        160        170        180 
MEDSVTVKPN LCKPEVNRQR PVHFFAVYDG HGGSQVSTLC STTMHTFVKE ELEQNLEEEE 

       190        200        210        220        230        240 
EGSENDVVER KWRGVMKRSF KRMDEMATST CVCGTSVPLC NCDPREAAIS GSTAVTAVLT 

       250        260        270        280        290        300 
HDHIIVANTG DSRAVLCRNG MAIPLSNDHK PDRPDERARI EAAGGRVLVV DGARVEGILA 

       310        320        330        340        350        360 
TSRAIGDRYL KPMVAWEPEV TFMRRESGDE CLVLASDGLW DVLSSQLACD IARFCLREET 

       370        380        390        400        410 
PSSLDLNRMA QEDDNDGEQN PSRSVLAATL LTRLALGRQS SDNISVVVID LKNSSQ 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones."
Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S.
DNA Res. 5:41-54(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"ABA-Hypersensitive Germination1 encodes a protein phosphatase 2C, an essential component of abscisic acid signaling in Arabidopsis seed."
Nishimura N., Yoshida T., Kitahata N., Asami T., Shinozaki K., Hirayama T.
Plant J. 50:935-949(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE, INDUCTION BY ABA, DISRUPTION PHENOTYPE.
[4]"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB010074 Genomic DNA. Translation: BAB11245.1.
CP002688 Genomic DNA. Translation: AED96123.1.
RefSeqNP_199989.1. NM_124555.2.
UniGeneAt.43187.

3D structure databases

ProteinModelPortalQ9FLI3.
SMRQ9FLI3. Positions 82-413.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid20495. 6 interactions.
DIPDIP-48990N.
IntActQ9FLI3. 1 interaction.
STRING3702.AT5G51760.1-P.

Proteomic databases

PaxDbQ9FLI3.
PRIDEQ9FLI3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G51760.1; AT5G51760.1; AT5G51760.
GeneID835250.
KEGGath:AT5G51760.

Organism-specific databases

TAIRAT5G51760.

Phylogenomic databases

eggNOGCOG0631.
HOGENOMHOG000233896.
InParanoidQ9FLI3.
KOK14497.
OMAYRTISTG.
PhylomeDBQ9FLI3.
ProtClustDBCLSN2916830.

Enzyme and pathway databases

BioCycARA:AT5G51760-MONOMER.

Gene expression databases

GenevestigatorQ9FLI3.

Family and domain databases

Gene3D3.60.40.10. 1 hit.
InterProIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00481. PP2C. 1 hit.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. SSF81606. 1 hit.
PROSITEPS01032. PP2C. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameP2C75_ARATH
AccessionPrimary (citable) accession number: Q9FLI3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: March 1, 2001
Last modified: March 19, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names