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Protein

Probable protein phosphatase 2C 75

Gene

AHG1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Negative regulator of abscisic acid (ABA) responses during seed germination.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi149Manganese 1By similarity1
Metal bindingi149Manganese 2By similarity1
Metal bindingi150Manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi337Manganese 2By similarity1
Metal bindingi402Manganese 2By similarity1

GO - Molecular functioni

GO - Biological processi

  • response to abscisic acid Source: TAIR

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandMagnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protein phosphatase 2C 75 (EC:3.1.3.16)
Short name:
AtPP2C75
Alternative name(s):
Protein ABA-HYPERSENSITIVE GERMINATION 1
Protein phosphatase 2C AHG1
Short name:
PP2C AHG1
Gene namesi
Name:AHG1
Ordered Locus Names:At5g51760
ORF Names:MIO24.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRilocus:2165371. AT5G51760.

Pathology & Biotechi

Disruption phenotypei

Hypersensitivity to ABA, salt and sucrose during seed germination.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003679951 – 416Probable protein phosphatase 2C 75Add BLAST416

Proteomic databases

PaxDbiQ9FLI3.

PTM databases

iPTMnetiQ9FLI3.

Expressioni

Developmental stagei

During germination, expressed in the embryo, vascular bundles and the root cap. In 6-day-old seedlings, expression in seed coat/endosperm disappears is detected only at the bases of the lateral root buds. Expressed in developing an mature siliques from 10 to 16 days after flowering (DAF).1 Publication

Inductioni

By ABA.1 Publication

Gene expression databases

GenevisibleiQ9FLI3. AT.

Interactioni

Protein-protein interaction databases

BioGridi20495. 17 interactors.
DIPiDIP-48990N.
IntActiQ9FLI3. 1 interactor.
STRINGi3702.AT5G51760.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FLI3.
SMRiQ9FLI3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini108 – 411PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST304

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi22 – 28Poly-Arg7

Sequence similaritiesi

Belongs to the PP2C family.Curated

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
HOGENOMiHOG000233896.
InParanoidiQ9FLI3.
KOiK14497.
OMAiAQECHRL.
OrthoDBiEOG09360DSN.
PhylomeDBiQ9FLI3.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiView protein in InterPro
IPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiView protein in Pfam
PF00481. PP2C. 1 hit.
SMARTiView protein in SMART
SM00332. PP2Cc. 1 hit.
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiView protein in PROSITE
PS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9FLI3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEIYRTIST GRGDDVSPTK CRERRRRRIE MRRQAAVFGE PSSSRNRDRT
60 70 80 90 100
DMEVYSSFDV PLRKQARRSE IGGLPADIGG FLAPPAASSC QKSEAPVWKG
110 120 130 140 150
EETEDEPLYG IVSVMGRSRK MEDSVTVKPN LCKPEVNRQR PVHFFAVYDG
160 170 180 190 200
HGGSQVSTLC STTMHTFVKE ELEQNLEEEE EGSENDVVER KWRGVMKRSF
210 220 230 240 250
KRMDEMATST CVCGTSVPLC NCDPREAAIS GSTAVTAVLT HDHIIVANTG
260 270 280 290 300
DSRAVLCRNG MAIPLSNDHK PDRPDERARI EAAGGRVLVV DGARVEGILA
310 320 330 340 350
TSRAIGDRYL KPMVAWEPEV TFMRRESGDE CLVLASDGLW DVLSSQLACD
360 370 380 390 400
IARFCLREET PSSLDLNRMA QEDDNDGEQN PSRSVLAATL LTRLALGRQS
410
SDNISVVVID LKNSSQ
Length:416
Mass (Da):46,043
Last modified:March 1, 2001 - v1
Checksum:i7272F86C929FF020
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010074 Genomic DNA. Translation: BAB11245.1.
CP002688 Genomic DNA. Translation: AED96123.1.
RefSeqiNP_199989.1. NM_124555.3.
UniGeneiAt.43187.

Genome annotation databases

EnsemblPlantsiAT5G51760.1; AT5G51760.1; AT5G51760.
GeneIDi835250.
GrameneiAT5G51760.1; AT5G51760.1; AT5G51760.
KEGGiath:AT5G51760.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010074 Genomic DNA. Translation: BAB11245.1.
CP002688 Genomic DNA. Translation: AED96123.1.
RefSeqiNP_199989.1. NM_124555.3.
UniGeneiAt.43187.

3D structure databases

ProteinModelPortaliQ9FLI3.
SMRiQ9FLI3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi20495. 17 interactors.
DIPiDIP-48990N.
IntActiQ9FLI3. 1 interactor.
STRINGi3702.AT5G51760.1.

PTM databases

iPTMnetiQ9FLI3.

Proteomic databases

PaxDbiQ9FLI3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G51760.1; AT5G51760.1; AT5G51760.
GeneIDi835250.
GrameneiAT5G51760.1; AT5G51760.1; AT5G51760.
KEGGiath:AT5G51760.

Organism-specific databases

AraportiAT5G51760.
TAIRilocus:2165371. AT5G51760.

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
HOGENOMiHOG000233896.
InParanoidiQ9FLI3.
KOiK14497.
OMAiAQECHRL.
OrthoDBiEOG09360DSN.
PhylomeDBiQ9FLI3.

Miscellaneous databases

PROiQ9FLI3.

Gene expression databases

GenevisibleiQ9FLI3. AT.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiView protein in InterPro
IPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiView protein in Pfam
PF00481. PP2C. 1 hit.
SMARTiView protein in SMART
SM00332. PP2Cc. 1 hit.
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiView protein in PROSITE
PS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiP2C75_ARATH
AccessioniPrimary (citable) accession number: Q9FLI3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: March 1, 2001
Last modified: February 15, 2017
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.