Q9FLI3 (P2C75_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable protein phosphatase 2C 75
Short name=AtPP2C75
EC=3.1.3.16

Alternative name(s):
Protein ABA-HYPERSENSITIVE GERMINATION 1
Protein phosphatase 2C AHG1
Short name=PP2C AHG1

Gene names

Name:	AHG1
Ordered Locus Names:	At5g51760
ORF Names:	MIO24.11

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Negative regulator of abscisic acid (ABA) responses during seed germination. Ref.3
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Developmental stage	During germination, expressed in the embryo, vascular bundles and the root cap. In 6-day-old seedlings, expression in seed coat/endosperm disappears is detected only at the bases of the lateral root buds. Expressed in developing an mature siliques from 10 to 16 days after flowering (DAF). Ref.3
Induction	By ABA. Ref.3
Disruption phenotype	Hypersensitivity to ABA, salt and sucrose during seed germination. Ref.3
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.

Ontologies

Keywords
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein dephosphorylation Inferred from electronic annotation. Source: InterPro response to abscisic acid stimulus Inferred from mutant phenotype Ref.3. Source: TAIR
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	Probable protein phosphatase 2C 75		PRO_0000367995
Regions
	Domain	107 – 404	298	PP2C-like
	Compositional bias	22 – 28	7	Poly-Arg
Sites
	Metal binding	149	1	Manganese 1 By similarity
	Metal binding	149	1	Manganese 2 By similarity
	Metal binding	150	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	337	1	Manganese 2 By similarity
	Metal binding	402	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9FLI3 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 7272F86C929FF020	FASTA	416	46,043
	10 20 30 40 50 60 MTEIYRTIST GRGDDVSPTK CRERRRRRIE MRRQAAVFGE PSSSRNRDRT DMEVYSSFDV 70 80 90 100 110 120 PLRKQARRSE IGGLPADIGG FLAPPAASSC QKSEAPVWKG EETEDEPLYG IVSVMGRSRK 130 140 150 160 170 180 MEDSVTVKPN LCKPEVNRQR PVHFFAVYDG HGGSQVSTLC STTMHTFVKE ELEQNLEEEE 190 200 210 220 230 240 EGSENDVVER KWRGVMKRSF KRMDEMATST CVCGTSVPLC NCDPREAAIS GSTAVTAVLT 250 260 270 280 290 300 HDHIIVANTG DSRAVLCRNG MAIPLSNDHK PDRPDERARI EAAGGRVLVV DGARVEGILA 310 320 330 340 350 360 TSRAIGDRYL KPMVAWEPEV TFMRRESGDE CLVLASDGLW DVLSSQLACD IARFCLREET 370 380 390 400 410 PSSLDLNRMA QEDDNDGEQN PSRSVLAATL LTRLALGRQS SDNISVVVID LKNSSQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones." Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S. DNA Res. 5:41-54(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"ABA-Hypersensitive Germination1 encodes a protein phosphatase 2C, an essential component of abscisic acid signaling in Arabidopsis seed." Nishimura N., Yoshida T., Kitahata N., Asami T., Shinozaki K., Hirayama T. Plant J. 50:935-949(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE, INDUCTION BY ABA, DISRUPTION PHENOTYPE.
[4]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB010074 Genomic DNA. Translation: BAB11245.1. CP002688 Genomic DNA. Translation: AED96123.1.
IPI	IPI00541471.
RefSeq	NP_199989.1. NM_124555.2.
UniGene	At.43187.
3D structure databases
ProteinModelPortal	Q9FLI3.
SMR	Q9FLI3. Positions 82-413.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48990N.
IntAct	Q9FLI3. 1 interaction.
STRING	3702.AT5G51760.1-P.
Proteomic databases
PaxDb	Q9FLI3.
PRIDE	Q9FLI3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G51760.1; AT5G51760.1; AT5G51760.
GeneID	835250.
KEGG	ath:AT5G51760.
Organism-specific databases
TAIR	At5g51760.
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000233896.
InParanoid	Q9FLI3.
KO	K14497.
OMA	EIYRTIS.
PhylomeDB	Q9FLI3.
ProtClustDB	CLSN2916830.
Gene expression databases
Genevestigator	Q9FLI3.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C75_ARATH

Accession

Primary (citable) accession number: Q9FLI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	March 1, 2001
Last modified:	May 29, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program