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Protein

Beta-D-xylosidase 4

Gene

BXL4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Beta-D-xylosidase showing an optimal efficiency with the natural substrate xylobiose.1 Publication

Catalytic activityi

Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini.1 Publication

Kineticsi

  1. KM=0.7 mM for p-nitrophenyl-beta-D-xylopyranoside1 Publication

    pH dependencei

    Optimum pH is 4.7.1 Publication

    Temperature dependencei

    Optimum temperature is 60 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei308By similarity1

    GO - Molecular functioni

    • alpha-L-arabinofuranosidase activity Source: GO_Central
    • xylan 1,4-beta-xylosidase activity Source: TAIR

    GO - Biological processi

    • arabinan catabolic process Source: GO_Central
    • systemic acquired resistance Source: TAIR
    • xylan catabolic process Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT5G64570-MONOMER.
    BRENDAi3.2.1.37. 399.
    SABIO-RKQ9FLG1.

    Protein family/group databases

    CAZyiGH3. Glycoside Hydrolase Family 3.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-D-xylosidase 4 (EC:3.2.1.37)
    Short name:
    AtBXL4
    Gene namesi
    Name:BXL4
    Ordered Locus Names:At5g64570
    ORF Names:MUB3.9
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    TAIRiAT5G64570.

    Subcellular locationi

    GO - Cellular componenti

    • apoplast Source: TAIR
    • cell wall Source: TAIR
    • plant-type cell wall Source: GO_Central
    • proteinaceous extracellular matrix Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Extracellular matrix, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 38Sequence analysisAdd BLAST38
    ChainiPRO_000038405939 – 784Beta-D-xylosidase 4Add BLAST746

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi141N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi441N-linked (GlcNAc...)Sequence analysis1

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9FLG1.
    PRIDEiQ9FLG1.

    Expressioni

    Tissue specificityi

    Expressed in flowers, siliques and the apical part of the stems.2 Publications

    Gene expression databases

    GenevisibleiQ9FLG1. AT.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT5G64570.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FLG1.
    SMRiQ9FLG1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 3 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG410IE0I. Eukaryota.
    COG1472. LUCA.
    HOGENOMiHOG000031216.
    InParanoidiQ9FLG1.
    KOiK15920.
    OMAiHCENAVS.
    OrthoDBiEOG0936046Q.
    PhylomeDBiQ9FLG1.

    Family and domain databases

    Gene3Di3.20.20.300. 1 hit.
    3.40.50.1700. 1 hit.
    InterProiIPR026891. Fn3-like.
    IPR026892. Glyco_hydro_3.
    IPR002772. Glyco_hydro_3_C.
    IPR001764. Glyco_hydro_3_N.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR30620. PTHR30620. 1 hit.
    PfamiPF14310. Fn3-like. 1 hit.
    PF00933. Glyco_hydro_3. 1 hit.
    PF01915. Glyco_hydro_3_C. 1 hit.
    [Graphical view]
    PRINTSiPR00133. GLHYDRLASE3.
    SMARTiSM01217. Fn3_like. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF52279. SSF52279. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9FLG1-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MGSSSPLTRR NRAPPSSVSS VYLIFLCFFL YFLNFSNAQS SPVFACDVAA
    60 70 80 90 100
    NPSLAAYGFC NTVLKIEYRV ADLVARLTLQ EKIGFLVSKA NGVTRLGIPT
    110 120 130 140 150
    YEWWSEALHG VSYIGPGTHF SSQVPGATSF PQVILTAASF NVSLFQAIGK
    160 170 180 190 200
    VVSTEARAMY NVGLAGLTYW SPNVNIFRDP RWGRGQETPG EDPLLASKYA
    210 220 230 240 250
    SGYVKGLQET DGGDSNRLKV AACCKHYTAY DVDNWKGVER YSFNAVVTQQ
    260 270 280 290 300
    DMDDTYQPPF KSCVVDGNVA SVMCSYNQVN GKPTCADPDL LSGVIRGEWK
    310 320 330 340 350
    LNGYIVSDCD SVDVLYKNQH YTKTPAEAAA ISILAGLDLN CGSFLGQHTE
    360 370 380 390 400
    EAVKSGLVNE AAIDKAISNN FLTLMRLGFF DGNPKNQIYG GLGPTDVCTS
    410 420 430 440 450
    ANQELAADAA RQGIVLLKNT GCLPLSPKSI KTLAVIGPNA NVTKTMIGNY
    460 470 480 490 500
    EGTPCKYTTP LQGLAGTVST TYLPGCSNVA CAVADVAGAT KLAATADVSV
    510 520 530 540 550
    LVIGADQSIE AESRDRVDLH LPGQQQELVI QVAKAAKGPV LLVIMSGGGF
    560 570 580 590 600
    DITFAKNDPK IAGILWVGYP GEAGGIAIAD IIFGRYNPSG KLPMTWYPQS
    610 620 630 640 650
    YVEKVPMTIM NMRPDKASGY PGRTYRFYTG ETVYAFGDGL SYTKFSHTLV
    660 670 680 690 700
    KAPSLVSLGL EENHVCRSSE CQSLDAIGPH CENAVSGGGS AFEVHIKVRN
    710 720 730 740 750
    GGDREGIHTV FLFTTPPAIH GSPRKHLVGF EKIRLGKREE AVVRFKVEIC
    760 770 780
    KDLSVVDEIG KRKIGLGKHL LHVGDLKHSL SIRI
    Length:784
    Mass (Da):84,308
    Last modified:March 1, 2001 - v1
    Checksum:i52D404092378771A
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti263C → Y in BAD94481 (Ref. 3) Curated1
    Sequence conflicti520H → R in BAD94481 (Ref. 3) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB010076 Genomic DNA. Translation: BAB11424.1.
    CP002688 Genomic DNA. Translation: AED97922.1.
    AK221967 mRNA. Translation: BAD94481.1.
    RefSeqiNP_201262.1. NM_125853.3.
    UniGeneiAt.21526.
    At.73119.

    Genome annotation databases

    EnsemblPlantsiAT5G64570.1; AT5G64570.1; AT5G64570.
    GeneIDi836578.
    GrameneiAT5G64570.1; AT5G64570.1; AT5G64570.
    KEGGiath:AT5G64570.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB010076 Genomic DNA. Translation: BAB11424.1.
    CP002688 Genomic DNA. Translation: AED97922.1.
    AK221967 mRNA. Translation: BAD94481.1.
    RefSeqiNP_201262.1. NM_125853.3.
    UniGeneiAt.21526.
    At.73119.

    3D structure databases

    ProteinModelPortaliQ9FLG1.
    SMRiQ9FLG1.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT5G64570.1.

    Protein family/group databases

    CAZyiGH3. Glycoside Hydrolase Family 3.

    Proteomic databases

    PaxDbiQ9FLG1.
    PRIDEiQ9FLG1.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT5G64570.1; AT5G64570.1; AT5G64570.
    GeneIDi836578.
    GrameneiAT5G64570.1; AT5G64570.1; AT5G64570.
    KEGGiath:AT5G64570.

    Organism-specific databases

    TAIRiAT5G64570.

    Phylogenomic databases

    eggNOGiENOG410IE0I. Eukaryota.
    COG1472. LUCA.
    HOGENOMiHOG000031216.
    InParanoidiQ9FLG1.
    KOiK15920.
    OMAiHCENAVS.
    OrthoDBiEOG0936046Q.
    PhylomeDBiQ9FLG1.

    Enzyme and pathway databases

    BioCyciARA:AT5G64570-MONOMER.
    BRENDAi3.2.1.37. 399.
    SABIO-RKQ9FLG1.

    Miscellaneous databases

    PROiQ9FLG1.

    Gene expression databases

    GenevisibleiQ9FLG1. AT.

    Family and domain databases

    Gene3Di3.20.20.300. 1 hit.
    3.40.50.1700. 1 hit.
    InterProiIPR026891. Fn3-like.
    IPR026892. Glyco_hydro_3.
    IPR002772. Glyco_hydro_3_C.
    IPR001764. Glyco_hydro_3_N.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR30620. PTHR30620. 1 hit.
    PfamiPF14310. Fn3-like. 1 hit.
    PF00933. Glyco_hydro_3. 1 hit.
    PF01915. Glyco_hydro_3_C. 1 hit.
    [Graphical view]
    PRINTSiPR00133. GLHYDRLASE3.
    SMARTiSM01217. Fn3_like. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF52279. SSF52279. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiBXL4_ARATH
    AccessioniPrimary (citable) accession number: Q9FLG1
    Secondary accession number(s): Q56WR7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: March 1, 2001
    Last modified: November 30, 2016
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Might be processed at the C-terminus.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.