Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Laccase-12

Gene

LAC12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Lignin degradation and detoxification of lignin-derived products.By similarity

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.

Cofactori

Cu cationBy similarityNote: Binds 4 Cu cations per monomer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi82Copper 1; type 2By similarity1
Metal bindingi84Copper 2; type 3By similarity1
Metal bindingi127Copper 2; type 3By similarity1
Metal bindingi129Copper 3; type 3By similarity1
Metal bindingi466Copper 4; type 1By similarity1
Metal bindingi469Copper 1; type 2By similarity1
Metal bindingi471Copper 3; type 3By similarity1
Metal bindingi528Copper 3; type 3By similarity1
Metal bindingi529Copper 4; type 1By similarity1
Metal bindingi530Copper 2; type 3By similarity1
Metal bindingi534Copper 4; type 1By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lignin degradation

Keywords - Ligandi

Copper, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G05390-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Laccase-12 (EC:1.10.3.2)
Alternative name(s):
Benzenediol:oxygen oxidoreductase 12
Diphenol oxidase 12
Urishiol oxidase 12
Gene namesi
Name:LAC12
Ordered Locus Names:At5g05390
ORF Names:K18I23.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G05390.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000028364025 – 565Laccase-12Add BLAST541

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi78N-linked (GlcNAc...)Sequence analysis1
Glycosylationi187N-linked (GlcNAc...)Sequence analysis1
Glycosylationi203N-linked (GlcNAc...)Sequence analysis1
Glycosylationi298N-linked (GlcNAc...)Sequence analysis1
Glycosylationi325N-linked (GlcNAc...)Sequence analysis1
Glycosylationi377N-linked (GlcNAc...)Sequence analysis1
Glycosylationi387N-linked (GlcNAc...)Sequence analysis1
Glycosylationi395N-linked (GlcNAc...)Sequence analysis1
Glycosylationi428N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9FLB5.

Expressioni

Tissue specificityi

Predominantly expressed in the inflorescence stem.2 Publications

Gene expression databases

GenevisibleiQ9FLB5. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G05390.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FLB5.
SMRiQ9FLB5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 148Plastocyanin-like 1Add BLAST117
Domaini158 – 310Plastocyanin-like 2Add BLAST153
Domaini413 – 549Plastocyanin-like 3Add BLAST137

Sequence similaritiesi

Belongs to the multicopper oxidase family.Curated
Contains 3 plastocyanin-like domains.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG1263. Eukaryota.
COG2132. LUCA.
HOGENOMiHOG000241916.
InParanoidiQ9FLB5.
KOiK05909.
OMAiKRYYIAA.
OrthoDBiEOG093605O6.
PhylomeDBiQ9FLB5.

Family and domain databases

Gene3Di2.60.40.420. 3 hits.
InterProiIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR033138. Cu_oxidase_CS.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
IPR017761. Laccase.
[Graphical view]
PfamiPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 4 hits.
TIGRFAMsiTIGR03389. laccase. 1 hit.
PROSITEiPS00079. MULTICOPPER_OXIDASE1. 1 hit.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FLB5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTVHTFSIL LFFCSLFSAS LIIAKVQHHD FVIQETPVKR LCKTRNAITV
60 70 80 90 100
NGMFPGPTLE VNNGDTLEVK VHNRARYNIT IHWHGVRQIR TGWADGPEFV
110 120 130 140 150
TQCPIRPGKS YTYRFTIQGQ EGTLWWHAHS SWLRATVYGA LIIHPTPGSS
160 170 180 190 200
FPFPKPDRQT ALMLGEWWNA NPVDVINQAT RTGAAPNISD AYTINGQPGD
210 220 230 240 250
LYNCSTKETV VVPINSGETS LLRVINAALN QPLFFTVANH KLTVVGADAS
260 270 280 290 300
YLKPFTTKVL MLGPGQTTDV LLTADQPPKR YYIAARAYQS AQNAPFDNTT
310 320 330 340 350
TTAILQYKKT TTTSKPIMPV LPAFNDTNTV TSFSRKFKSL RNVVVPKTID
360 370 380 390 400
DNLFFTIGLG LDNCPKKFPK SRCQGLNGTR FTASMNNVSF VLPSNFSLLQ
410 420 430 440 450
AHSNGIPGVF TTDFPSKPPV KFDYTGNNIS RALFQPVKGT KLYKLKYGSR
460 470 480 490 500
VQVVLQDTNI VTSENHPIHL HGYDFYIVGE GFGNFNPKKD TSKFNLVDPP
510 520 530 540 550
LRNTVAVPVN GWAVIRFVAD NPGVWLMHCH LDVHIKWGLA MAFLVDNGVG
560
ELETLEAPPH DLPIC
Length:565
Mass (Da):62,734
Last modified:March 1, 2001 - v1
Checksum:i15DA9DDD5CA8932E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010692 Genomic DNA. Translation: BAB09982.1.
CP002688 Genomic DNA. Translation: AED90868.1.
RefSeqiNP_196158.1. NM_120621.2.

Genome annotation databases

EnsemblPlantsiAT5G05390.1; AT5G05390.1; AT5G05390.
GeneIDi830421.
GrameneiAT5G05390.1; AT5G05390.1; AT5G05390.
KEGGiath:AT5G05390.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010692 Genomic DNA. Translation: BAB09982.1.
CP002688 Genomic DNA. Translation: AED90868.1.
RefSeqiNP_196158.1. NM_120621.2.

3D structure databases

ProteinModelPortaliQ9FLB5.
SMRiQ9FLB5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G05390.1.

Proteomic databases

PaxDbiQ9FLB5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G05390.1; AT5G05390.1; AT5G05390.
GeneIDi830421.
GrameneiAT5G05390.1; AT5G05390.1; AT5G05390.
KEGGiath:AT5G05390.

Organism-specific databases

TAIRiAT5G05390.

Phylogenomic databases

eggNOGiKOG1263. Eukaryota.
COG2132. LUCA.
HOGENOMiHOG000241916.
InParanoidiQ9FLB5.
KOiK05909.
OMAiKRYYIAA.
OrthoDBiEOG093605O6.
PhylomeDBiQ9FLB5.

Enzyme and pathway databases

BioCyciARA:AT5G05390-MONOMER.

Miscellaneous databases

PROiQ9FLB5.

Gene expression databases

GenevisibleiQ9FLB5. AT.

Family and domain databases

Gene3Di2.60.40.420. 3 hits.
InterProiIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR033138. Cu_oxidase_CS.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
IPR017761. Laccase.
[Graphical view]
PfamiPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 4 hits.
TIGRFAMsiTIGR03389. laccase. 1 hit.
PROSITEiPS00079. MULTICOPPER_OXIDASE1. 1 hit.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLAC12_ARATH
AccessioniPrimary (citable) accession number: Q9FLB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.