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Q9FLB5

- LAC12_ARATH

UniProt

Q9FLB5 - LAC12_ARATH

Protein

Laccase-12

Gene

LAC12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Lignin degradation and detoxification of lignin-derived products.By similarity

    Catalytic activityi

    4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.

    Cofactori

    Binds 4 copper ions per monomer.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi82 – 821Copper 1; type 2By similarity
    Metal bindingi84 – 841Copper 2; type 3By similarity
    Metal bindingi127 – 1271Copper 2; type 3By similarity
    Metal bindingi129 – 1291Copper 3; type 3By similarity
    Metal bindingi466 – 4661Copper 4; type 1By similarity
    Metal bindingi469 – 4691Copper 1; type 2By similarity
    Metal bindingi471 – 4711Copper 3; type 3By similarity
    Metal bindingi528 – 5281Copper 3; type 3By similarity
    Metal bindingi529 – 5291Copper 4; type 1By similarity
    Metal bindingi530 – 5301Copper 2; type 3By similarity
    Metal bindingi534 – 5341Copper 4; type 1By similarity

    GO - Molecular functioni

    1. copper ion binding Source: InterPro
    2. hydroquinone:oxygen oxidoreductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. lignin catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Lignin degradation

    Keywords - Ligandi

    Copper, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G05390-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Laccase-12 (EC:1.10.3.2)
    Alternative name(s):
    Benzenediol:oxygen oxidoreductase 12
    Diphenol oxidase 12
    Urishiol oxidase 12
    Gene namesi
    Name:LAC12
    Ordered Locus Names:At5g05390
    ORF Names:K18I23.20
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G05390.

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Apoplast, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 565541Laccase-12PRO_0000283640Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi78 – 781N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi187 – 1871N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi203 – 2031N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi298 – 2981N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi325 – 3251N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi377 – 3771N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi387 – 3871N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi395 – 3951N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi428 – 4281N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9FLB5.
    PRIDEiQ9FLB5.

    Expressioni

    Tissue specificityi

    Predominantly expressed in the inflorescence stem.2 Publications

    Gene expression databases

    GenevestigatoriQ9FLB5.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT5G05390.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FLB5.
    SMRiQ9FLB5. Positions 24-544.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini32 – 148117Plastocyanin-like 1Add
    BLAST
    Domaini158 – 310153Plastocyanin-like 2Add
    BLAST
    Domaini413 – 549137Plastocyanin-like 3Add
    BLAST

    Sequence similaritiesi

    Belongs to the multicopper oxidase family.Curated
    Contains 3 plastocyanin-like domains.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG2132.
    HOGENOMiHOG000241916.
    InParanoidiQ9FLB5.
    OMAiNISRALF.
    PhylomeDBiQ9FLB5.

    Family and domain databases

    Gene3Di2.60.40.420. 3 hits.
    InterProiIPR001117. Cu-oxidase.
    IPR011706. Cu-oxidase_2.
    IPR011707. Cu-oxidase_3.
    IPR002355. Cu_oxidase_Cu_BS.
    IPR008972. Cupredoxin.
    IPR017761. Laccase.
    [Graphical view]
    PfamiPF00394. Cu-oxidase. 1 hit.
    PF07731. Cu-oxidase_2. 1 hit.
    PF07732. Cu-oxidase_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF49503. SSF49503. 4 hits.
    TIGRFAMsiTIGR03389. laccase. 1 hit.
    PROSITEiPS00079. MULTICOPPER_OXIDASE1. 1 hit.
    PS00080. MULTICOPPER_OXIDASE2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9FLB5-1 [UniParc]FASTAAdd to Basket

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    MTTVHTFSIL LFFCSLFSAS LIIAKVQHHD FVIQETPVKR LCKTRNAITV    50
    NGMFPGPTLE VNNGDTLEVK VHNRARYNIT IHWHGVRQIR TGWADGPEFV 100
    TQCPIRPGKS YTYRFTIQGQ EGTLWWHAHS SWLRATVYGA LIIHPTPGSS 150
    FPFPKPDRQT ALMLGEWWNA NPVDVINQAT RTGAAPNISD AYTINGQPGD 200
    LYNCSTKETV VVPINSGETS LLRVINAALN QPLFFTVANH KLTVVGADAS 250
    YLKPFTTKVL MLGPGQTTDV LLTADQPPKR YYIAARAYQS AQNAPFDNTT 300
    TTAILQYKKT TTTSKPIMPV LPAFNDTNTV TSFSRKFKSL RNVVVPKTID 350
    DNLFFTIGLG LDNCPKKFPK SRCQGLNGTR FTASMNNVSF VLPSNFSLLQ 400
    AHSNGIPGVF TTDFPSKPPV KFDYTGNNIS RALFQPVKGT KLYKLKYGSR 450
    VQVVLQDTNI VTSENHPIHL HGYDFYIVGE GFGNFNPKKD TSKFNLVDPP 500
    LRNTVAVPVN GWAVIRFVAD NPGVWLMHCH LDVHIKWGLA MAFLVDNGVG 550
    ELETLEAPPH DLPIC 565
    Length:565
    Mass (Da):62,734
    Last modified:March 1, 2001 - v1
    Checksum:i15DA9DDD5CA8932E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB010692 Genomic DNA. Translation: BAB09982.1.
    CP002688 Genomic DNA. Translation: AED90868.1.
    RefSeqiNP_196158.1. NM_120621.1.
    UniGeneiAt.54730.

    Genome annotation databases

    EnsemblPlantsiAT5G05390.1; AT5G05390.1; AT5G05390.
    GeneIDi830421.
    KEGGiath:AT5G05390.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB010692 Genomic DNA. Translation: BAB09982.1 .
    CP002688 Genomic DNA. Translation: AED90868.1 .
    RefSeqi NP_196158.1. NM_120621.1.
    UniGenei At.54730.

    3D structure databases

    ProteinModelPortali Q9FLB5.
    SMRi Q9FLB5. Positions 24-544.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT5G05390.1-P.

    Proteomic databases

    PaxDbi Q9FLB5.
    PRIDEi Q9FLB5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G05390.1 ; AT5G05390.1 ; AT5G05390 .
    GeneIDi 830421.
    KEGGi ath:AT5G05390.

    Organism-specific databases

    TAIRi AT5G05390.

    Phylogenomic databases

    eggNOGi COG2132.
    HOGENOMi HOG000241916.
    InParanoidi Q9FLB5.
    OMAi NISRALF.
    PhylomeDBi Q9FLB5.

    Enzyme and pathway databases

    BioCyci ARA:AT5G05390-MONOMER.

    Gene expression databases

    Genevestigatori Q9FLB5.

    Family and domain databases

    Gene3Di 2.60.40.420. 3 hits.
    InterProi IPR001117. Cu-oxidase.
    IPR011706. Cu-oxidase_2.
    IPR011707. Cu-oxidase_3.
    IPR002355. Cu_oxidase_Cu_BS.
    IPR008972. Cupredoxin.
    IPR017761. Laccase.
    [Graphical view ]
    Pfami PF00394. Cu-oxidase. 1 hit.
    PF07731. Cu-oxidase_2. 1 hit.
    PF07732. Cu-oxidase_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49503. SSF49503. 4 hits.
    TIGRFAMsi TIGR03389. laccase. 1 hit.
    PROSITEi PS00079. MULTICOPPER_OXIDASE1. 1 hit.
    PS00080. MULTICOPPER_OXIDASE2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones."
      Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S.
      DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Gene structure and molecular analysis of the laccase-like multicopper oxidase (LMCO) gene family in Arabidopsis thaliana."
      McCaig B.C., Meagher R.B., Dean J.F.D.
      Planta 221:619-636(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    4. "Mutant identification and characterization of the laccase gene family in Arabidopsis."
      Cai X., Davis E.J., Ballif J., Liang M., Bushman E., Haroldsen V., Torabinejad J., Wu Y.
      J. Exp. Bot. 57:2563-2569(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiLAC12_ARATH
    AccessioniPrimary (citable) accession number: Q9FLB5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2007
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3