Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA replication licensing factor MCM3

Gene

MCM3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable component of the MCM2-7 complex (MCM complex) that may function as a DNA helicase and which is essential to undergo a single round of replication initiation and elongation per cell cycle in eukaryotic cells.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi335 – 342ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • DNA replication initiation Source: InterPro
  • DNA unwinding involved in DNA replication Source: TAIR

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processCell cycle, DNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-68867. Assembly of the pre-replicative complex.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-68962. Activation of the pre-replicative complex.
R-ATH-69052. Switching of origins to a post-replicative state.
R-ATH-69300. Removal of licensing factors from origins.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM3 (EC:3.6.4.12)
Alternative name(s):
Minichromosome maintenance protein 3
Short name:
AtMCM3
Gene namesi
Name:MCM3
Ordered Locus Names:At5g46280
ORF Names:MPL12.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRilocus:2170418. AT5G46280.

Subcellular locationi

GO - Cellular componenti

  • MCM complex Source: InterPro
  • THO complex Source: UniProtKB

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001940971 – 776DNA replication licensing factor MCM3Add BLAST776

Proteomic databases

PaxDbiQ9FL33.

PTM databases

iPTMnetiQ9FL33.

Expressioni

Tissue specificityi

Expressed in root meristem, shoot apex and flower buds.2 Publications

Gene expression databases

GenevisibleiQ9FL33. AT.

Interactioni

Subunit structurei

Component of the minichromosome maintenance (MCM) complex, a heterotetramer composed of MCM2, MCM3, MCM4, MCM5, MCM6 and MCM7. Interacts with ETG1.1 Publication

Protein-protein interaction databases

BioGridi19919. 2 interactors.
IntActiQ9FL33. 1 interactor.
MINTiMINT-6799295.
STRINGi3702.AT5G46280.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FL33.
SMRiQ9FL33.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini285 – 491MCMAdd BLAST207

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi467 – 470Arginine finger4

Sequence similaritiesi

Belongs to the MCM family.Curated

Phylogenomic databases

eggNOGiKOG0479. Eukaryota.
COG1241. LUCA.
HOGENOMiHOG000224126.
InParanoidiQ9FL33.
KOiK02541.
OMAiDINCLLV.
OrthoDBiEOG093602WH.
PhylomeDBiQ9FL33.

Family and domain databases

InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR031327. MCM.
IPR008046. Mcm3.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR033762. MCM_OB.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
PF17207. MCM_OB. 1 hit.
PRINTSiPR01657. MCMFAMILY.
PR01659. MCMPROTEIN3.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiView protein in PROSITE
PS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9FL33-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVPEETRLR HKRDFIQFLD SMYMEEIKAL VHQKRHRLII NISDIHHHFR
60 70 80 90 100
EVASRILKNP NEYMQSFCDA ATEATRAIDP KYLKEGELVL VGFEGYFVSR
110 120 130 140 150
VVTPRELLSD FIGSMVCVEG IVTKCSLVRP KVVKSVHFCP STGEFTNRDY
160 170 180 190 200
RDITSHAGLP TGSVYPTRDD KGNLLVTEYG LCKYKDHQTL SIQEVPENAA
210 220 230 240 250
PGQLPRSVDV IAEDDLVDSC KPGDRVSVFG IYKALPGKSK GSVNGVFRTI
260 270 280 290 300
LIANNIALLN KEANAPIYTK QDLDNIKNIA RRDDAFDLLA RSLAPSIYGH
310 320 330 340 350
AWIKKAVVLL MLGGVEKNLK NGTHLRGDIN MMMVGDPSVA KSQLLRAIMN
360 370 380 390 400
IAPLAISTTG RGSSGVGLTA AVTSDQETGE RRLEAGAMVL ADKGIVCIDE
410 420 430 440 450
FDKMNDQDRV AIHEVMEQQT VTIAKAGIHA SLNARCSVVA AANPIYGTYD
460 470 480 490 500
RSLTPTKNIG LPDSLLSRFD LLFIVLDQMD AGIDSMISEH VLRMHRYKND
510 520 530 540 550
RGEAGPDGSL PYAREDNAES EMFVKYNQTL HGKKKRGQTH DKTLTIKFLK
560 570 580 590 600
KYIHYAKHRI TPKLTDEASE RIAEAYADLR NAGSDTKTGG TLPITARTLE
610 620 630 640 650
TIIRLATAHA KMKLSSEVTK ADAEAALKLM NFAIYHQELT EMDDREQEER
660 670 680 690 700
QREQAEQERT PSGRRGNQRR NNEDGAENDT ANVDSETADP MEVDEPSVEQ
710 720 730 740 750
FSGTVSAARI ETFERVFGQH MRTHRLDDIS IADIETVVNN NGVGASRYSA
760 770
DEIMALLEKL QDDNKVMISD GKVHII
Length:776
Mass (Da):86,357
Last modified:March 1, 2001 - v1
Checksum:i48EB068DC2AA8894
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti502G → S in CAA03887 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000058 mRNA. Translation: CAA03887.1.
AB010698 Genomic DNA. Translation: BAB11083.1.
CP002688 Genomic DNA. Translation: AED95362.1.
PIRiT52118.
RefSeqiNP_199440.1. NM_123997.3.
UniGeneiAt.168.

Genome annotation databases

EnsemblPlantsiAT5G46280.1; AT5G46280.1; AT5G46280.
GeneIDi834670.
GrameneiAT5G46280.1; AT5G46280.1; AT5G46280.
KEGGiath:AT5G46280.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000058 mRNA. Translation: CAA03887.1.
AB010698 Genomic DNA. Translation: BAB11083.1.
CP002688 Genomic DNA. Translation: AED95362.1.
PIRiT52118.
RefSeqiNP_199440.1. NM_123997.3.
UniGeneiAt.168.

3D structure databases

ProteinModelPortaliQ9FL33.
SMRiQ9FL33.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19919. 2 interactors.
IntActiQ9FL33. 1 interactor.
MINTiMINT-6799295.
STRINGi3702.AT5G46280.1.

PTM databases

iPTMnetiQ9FL33.

Proteomic databases

PaxDbiQ9FL33.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G46280.1; AT5G46280.1; AT5G46280.
GeneIDi834670.
GrameneiAT5G46280.1; AT5G46280.1; AT5G46280.
KEGGiath:AT5G46280.

Organism-specific databases

AraportiAT5G46280.
TAIRilocus:2170418. AT5G46280.

Phylogenomic databases

eggNOGiKOG0479. Eukaryota.
COG1241. LUCA.
HOGENOMiHOG000224126.
InParanoidiQ9FL33.
KOiK02541.
OMAiDINCLLV.
OrthoDBiEOG093602WH.
PhylomeDBiQ9FL33.

Enzyme and pathway databases

ReactomeiR-ATH-68867. Assembly of the pre-replicative complex.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-68962. Activation of the pre-replicative complex.
R-ATH-69052. Switching of origins to a post-replicative state.
R-ATH-69300. Removal of licensing factors from origins.

Miscellaneous databases

PROiPR:Q9FL33.

Gene expression databases

GenevisibleiQ9FL33. AT.

Family and domain databases

InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR031327. MCM.
IPR008046. Mcm3.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR033762. MCM_OB.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
PF17207. MCM_OB. 1 hit.
PRINTSiPR01657. MCMFAMILY.
PR01659. MCMPROTEIN3.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiView protein in PROSITE
PS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMCM3_ARATH
AccessioniPrimary (citable) accession number: Q9FL33
Secondary accession number(s): O65400
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: March 1, 2001
Last modified: April 12, 2017
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.