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Protein

DNA replication licensing factor MCM3

Gene

MCM3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable component of the MCM2-7 complex (MCM complex) that may function as a DNA helicase and which is essential to undergo a single round of replication initiation and elongation per cell cycle in eukaryotic cells.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi335 – 3428ATPBy similarity

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • DNA replication initiation Source: InterPro
  • DNA unwinding involved in DNA replication Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-68867. Assembly of the pre-replicative complex.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-68962. Activation of the pre-replicative complex.
R-ATH-69052. Switching of origins to a post-replicative state.
R-ATH-69300. Removal of licensing factors from origins.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM3 (EC:3.6.4.12)
Alternative name(s):
Minichromosome maintenance protein 3
Short name:
AtMCM3
Gene namesi
Name:MCM3
Ordered Locus Names:At5g46280
ORF Names:MPL12.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G46280.

Subcellular locationi

GO - Cellular componenti

  • MCM complex Source: InterPro
  • THO complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 776776DNA replication licensing factor MCM3PRO_0000194097Add
BLAST

Proteomic databases

PaxDbiQ9FL33.
PRIDEiQ9FL33.

PTM databases

iPTMnetiQ9FL33.

Expressioni

Tissue specificityi

Expressed in root meristem, shoot apex and flower buds.2 Publications

Gene expression databases

GenevisibleiQ9FL33. AT.

Interactioni

Subunit structurei

Component of the minichromosome maintenance (MCM) complex, a heterotetramer composed of MCM2, MCM3, MCM4, MCM5, MCM6 and MCM7. Interacts with ETG1.1 Publication

Protein-protein interaction databases

BioGridi19919. 2 interactions.
IntActiQ9FL33. 1 interaction.
MINTiMINT-6799295.
STRINGi3702.AT5G46280.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FL33.
SMRiQ9FL33. Positions 4-635.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini285 – 491207MCMAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi467 – 4704Arginine finger

Sequence similaritiesi

Belongs to the MCM family.Curated
Contains 1 MCM domain.Curated

Phylogenomic databases

eggNOGiKOG0479. Eukaryota.
COG1241. LUCA.
HOGENOMiHOG000224126.
InParanoidiQ9FL33.
KOiK02541.
OMAiMAINRSD.
PhylomeDBiQ9FL33.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR031327. MCM.
IPR008046. Mcm3.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01659. MCMPROTEIN3.
SMARTiSM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FL33-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVPEETRLR HKRDFIQFLD SMYMEEIKAL VHQKRHRLII NISDIHHHFR
60 70 80 90 100
EVASRILKNP NEYMQSFCDA ATEATRAIDP KYLKEGELVL VGFEGYFVSR
110 120 130 140 150
VVTPRELLSD FIGSMVCVEG IVTKCSLVRP KVVKSVHFCP STGEFTNRDY
160 170 180 190 200
RDITSHAGLP TGSVYPTRDD KGNLLVTEYG LCKYKDHQTL SIQEVPENAA
210 220 230 240 250
PGQLPRSVDV IAEDDLVDSC KPGDRVSVFG IYKALPGKSK GSVNGVFRTI
260 270 280 290 300
LIANNIALLN KEANAPIYTK QDLDNIKNIA RRDDAFDLLA RSLAPSIYGH
310 320 330 340 350
AWIKKAVVLL MLGGVEKNLK NGTHLRGDIN MMMVGDPSVA KSQLLRAIMN
360 370 380 390 400
IAPLAISTTG RGSSGVGLTA AVTSDQETGE RRLEAGAMVL ADKGIVCIDE
410 420 430 440 450
FDKMNDQDRV AIHEVMEQQT VTIAKAGIHA SLNARCSVVA AANPIYGTYD
460 470 480 490 500
RSLTPTKNIG LPDSLLSRFD LLFIVLDQMD AGIDSMISEH VLRMHRYKND
510 520 530 540 550
RGEAGPDGSL PYAREDNAES EMFVKYNQTL HGKKKRGQTH DKTLTIKFLK
560 570 580 590 600
KYIHYAKHRI TPKLTDEASE RIAEAYADLR NAGSDTKTGG TLPITARTLE
610 620 630 640 650
TIIRLATAHA KMKLSSEVTK ADAEAALKLM NFAIYHQELT EMDDREQEER
660 670 680 690 700
QREQAEQERT PSGRRGNQRR NNEDGAENDT ANVDSETADP MEVDEPSVEQ
710 720 730 740 750
FSGTVSAARI ETFERVFGQH MRTHRLDDIS IADIETVVNN NGVGASRYSA
760 770
DEIMALLEKL QDDNKVMISD GKVHII
Length:776
Mass (Da):86,357
Last modified:March 1, 2001 - v1
Checksum:i48EB068DC2AA8894
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti502 – 5021G → S in CAA03887 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000058 mRNA. Translation: CAA03887.1.
AB010698 Genomic DNA. Translation: BAB11083.1.
CP002688 Genomic DNA. Translation: AED95362.1.
PIRiT52118.
RefSeqiNP_199440.1. NM_123997.2.
UniGeneiAt.168.

Genome annotation databases

EnsemblPlantsiAT5G46280.1; AT5G46280.1; AT5G46280.
GeneIDi834670.
GrameneiAT5G46280.1; AT5G46280.1; AT5G46280.
KEGGiath:AT5G46280.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000058 mRNA. Translation: CAA03887.1.
AB010698 Genomic DNA. Translation: BAB11083.1.
CP002688 Genomic DNA. Translation: AED95362.1.
PIRiT52118.
RefSeqiNP_199440.1. NM_123997.2.
UniGeneiAt.168.

3D structure databases

ProteinModelPortaliQ9FL33.
SMRiQ9FL33. Positions 4-635.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19919. 2 interactions.
IntActiQ9FL33. 1 interaction.
MINTiMINT-6799295.
STRINGi3702.AT5G46280.1.

PTM databases

iPTMnetiQ9FL33.

Proteomic databases

PaxDbiQ9FL33.
PRIDEiQ9FL33.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G46280.1; AT5G46280.1; AT5G46280.
GeneIDi834670.
GrameneiAT5G46280.1; AT5G46280.1; AT5G46280.
KEGGiath:AT5G46280.

Organism-specific databases

TAIRiAT5G46280.

Phylogenomic databases

eggNOGiKOG0479. Eukaryota.
COG1241. LUCA.
HOGENOMiHOG000224126.
InParanoidiQ9FL33.
KOiK02541.
OMAiMAINRSD.
PhylomeDBiQ9FL33.

Enzyme and pathway databases

ReactomeiR-ATH-68867. Assembly of the pre-replicative complex.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-68962. Activation of the pre-replicative complex.
R-ATH-69052. Switching of origins to a post-replicative state.
R-ATH-69300. Removal of licensing factors from origins.

Miscellaneous databases

PROiQ9FL33.

Gene expression databases

GenevisibleiQ9FL33. AT.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR031327. MCM.
IPR008046. Mcm3.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01659. MCMPROTEIN3.
SMARTiSM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression analysis of Arabidopsis thaliana MCM3 homolog during the cell cycle."
    Dodeman V.L., Phan T., Sabelli P., Bergounioux C.
    Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
    Tissue: Seedling.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones."
    Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Two E2F sites in the Arabidopsis MCM3 promoter have different roles in cell cycle activation and meristematic expression."
    Stevens R., Mariconti L., Rossignol P., Perennes C., Cella R., Bergounioux C.
    J. Biol. Chem. 277:32978-32984(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  5. "Genome-wide analysis of the core DNA replication machinery in the higher plants Arabidopsis and rice."
    Shultz R.W., Tatineni V.M., Hanley-Bowdoin L., Thompson W.F.
    Plant Physiol. 144:1697-1714(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  6. "The DNA replication checkpoint aids survival of plants deficient in the novel replisome factor ETG1."
    Takahashi N., Lammens T., Boudolf V., Maes S., Yoshizumi T., De Jaeger G., Witters E., Inze D., De Veylder L.
    EMBO J. 27:1840-1851(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, INTERACTION WITH ETG1.
  7. "Dynamic localization of the DNA replication proteins MCM5 and MCM7 in plants."
    Shultz R.W., Lee T.J., Allen G.C., Thompson W.F., Hanley-Bowdoin L.
    Plant Physiol. 150:658-669(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiMCM3_ARATH
AccessioniPrimary (citable) accession number: Q9FL33
Secondary accession number(s): O65400
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: March 1, 2001
Last modified: June 8, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.