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Protein

DNA replication licensing factor MCM3

Gene

MCM3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable component of the MCM2-7 complex (MCM complex) that may function as a DNA helicase and which is essential to undergo a single round of replication initiation and elongation per cell cycle in eukaryotic cells.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi335 – 342ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • DNA replication initiation Source: InterPro
  • DNA unwinding involved in DNA replication Source: TAIR

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processCell cycle, DNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-68867 Assembly of the pre-replicative complex
R-ATH-68949 Orc1 removal from chromatin
R-ATH-68962 Activation of the pre-replicative complex
R-ATH-69052 Switching of origins to a post-replicative state

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM3 (EC:3.6.4.12)
Alternative name(s):
Minichromosome maintenance protein 3
Short name:
AtMCM3
Gene namesi
Name:MCM3
Ordered Locus Names:At5g46280
ORF Names:MPL12.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G46280
TAIRilocus:2170418 AT5G46280

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001940971 – 776DNA replication licensing factor MCM3Add BLAST776

Proteomic databases

PaxDbiQ9FL33
PRIDEiQ9FL33

PTM databases

iPTMnetiQ9FL33

Expressioni

Tissue specificityi

Expressed in root meristem, shoot apex and flower buds.2 Publications

Gene expression databases

ExpressionAtlasiQ9FL33 baseline and differential
GenevisibleiQ9FL33 AT

Interactioni

Subunit structurei

Component of the minichromosome maintenance (MCM) complex, a heterotetramer composed of MCM2, MCM3, MCM4, MCM5, MCM6 and MCM7. Interacts with ETG1.1 Publication

Protein-protein interaction databases

BioGridi19919, 2 interactors
IntActiQ9FL33, 1 interactor
MINTiQ9FL33
STRINGi3702.AT5G46280.1

Structurei

3D structure databases

ProteinModelPortaliQ9FL33
SMRiQ9FL33
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini285 – 491MCMAdd BLAST207

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi467 – 470Arginine finger4

Sequence similaritiesi

Belongs to the MCM family.Curated

Phylogenomic databases

eggNOGiKOG0479 Eukaryota
COG1241 LUCA
HOGENOMiHOG000224126
InParanoidiQ9FL33
KOiK02541
OMAiYQSKVRE
OrthoDBiEOG093602WH
PhylomeDBiQ9FL33

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR031327 MCM
IPR008046 Mcm3
IPR018525 MCM_CS
IPR001208 MCM_dom
IPR027925 MCM_N
IPR033762 MCM_OB
IPR012340 NA-bd_OB-fold
IPR027417 P-loop_NTPase
PANTHERiPTHR11630 PTHR11630, 1 hit
PfamiView protein in Pfam
PF00493 MCM, 1 hit
PF14551 MCM_N, 1 hit
PF17207 MCM_OB, 1 hit
PRINTSiPR01657 MCMFAMILY
PR01659 MCMPROTEIN3
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00350 MCM, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS00847 MCM_1, 1 hit
PS50051 MCM_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9FL33-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVPEETRLR HKRDFIQFLD SMYMEEIKAL VHQKRHRLII NISDIHHHFR
60 70 80 90 100
EVASRILKNP NEYMQSFCDA ATEATRAIDP KYLKEGELVL VGFEGYFVSR
110 120 130 140 150
VVTPRELLSD FIGSMVCVEG IVTKCSLVRP KVVKSVHFCP STGEFTNRDY
160 170 180 190 200
RDITSHAGLP TGSVYPTRDD KGNLLVTEYG LCKYKDHQTL SIQEVPENAA
210 220 230 240 250
PGQLPRSVDV IAEDDLVDSC KPGDRVSVFG IYKALPGKSK GSVNGVFRTI
260 270 280 290 300
LIANNIALLN KEANAPIYTK QDLDNIKNIA RRDDAFDLLA RSLAPSIYGH
310 320 330 340 350
AWIKKAVVLL MLGGVEKNLK NGTHLRGDIN MMMVGDPSVA KSQLLRAIMN
360 370 380 390 400
IAPLAISTTG RGSSGVGLTA AVTSDQETGE RRLEAGAMVL ADKGIVCIDE
410 420 430 440 450
FDKMNDQDRV AIHEVMEQQT VTIAKAGIHA SLNARCSVVA AANPIYGTYD
460 470 480 490 500
RSLTPTKNIG LPDSLLSRFD LLFIVLDQMD AGIDSMISEH VLRMHRYKND
510 520 530 540 550
RGEAGPDGSL PYAREDNAES EMFVKYNQTL HGKKKRGQTH DKTLTIKFLK
560 570 580 590 600
KYIHYAKHRI TPKLTDEASE RIAEAYADLR NAGSDTKTGG TLPITARTLE
610 620 630 640 650
TIIRLATAHA KMKLSSEVTK ADAEAALKLM NFAIYHQELT EMDDREQEER
660 670 680 690 700
QREQAEQERT PSGRRGNQRR NNEDGAENDT ANVDSETADP MEVDEPSVEQ
710 720 730 740 750
FSGTVSAARI ETFERVFGQH MRTHRLDDIS IADIETVVNN NGVGASRYSA
760 770
DEIMALLEKL QDDNKVMISD GKVHII
Length:776
Mass (Da):86,357
Last modified:March 1, 2001 - v1
Checksum:i48EB068DC2AA8894
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti502G → S in CAA03887 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000058 mRNA Translation: CAA03887.1
AB010698 Genomic DNA Translation: BAB11083.1
CP002688 Genomic DNA Translation: AED95362.1
PIRiT52118
RefSeqiNP_199440.1, NM_123997.3
UniGeneiAt.168

Genome annotation databases

EnsemblPlantsiAT5G46280.1; AT5G46280.1; AT5G46280
GeneIDi834670
GrameneiAT5G46280.1; AT5G46280.1; AT5G46280
KEGGiath:AT5G46280

Similar proteinsi

Entry informationi

Entry nameiMCM3_ARATH
AccessioniPrimary (citable) accession number: Q9FL33
Secondary accession number(s): O65400
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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