Reviewed, UniProtKB/Swiss-Prot Q9FL16 (PER63_ARATH)

Names and origin

Protein names

Recommended name:
    Peroxidase 63
      Short name=Atperox P63
    EC=1.11.1.7
Alternative name(s):
    ATP26a

Gene names

Name:	PER63
Synonyms:	P63
Ordered Locus Names:	At5g40150
ORF Names:	MSN9.5, MSN9.50

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per subunit By similarity. Binds 2 calcium ions per subunit By similarity.
Subcellular location	Secreted By similarity.
Miscellaneous	There are 73 peroxidase genes in A.thaliana.
Sequence similarities	Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro peroxidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 328	301	Peroxidase 63		PRO_0000023728
Sites
	Active site	72	1	Proton acceptor By similarity
	Metal binding	73	1	Calcium 1 By similarity
	Metal binding	78	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	80	1	Calcium 1 By similarity
	Metal binding	82	1	Calcium 1 By similarity
	Metal binding	200	1	Iron (heme axial ligand) By similarity
	Metal binding	201	1	Calcium 2 By similarity
	Metal binding	248	1	Calcium 2 By similarity
	Metal binding	251	1	Calcium 2 By similarity
	Metal binding	256	1	Calcium 2 By similarity
	Binding site	170	1	Substrate; via carbonyl oxygen By similarity
	Site	68	1	Transition state stabilizer By similarity
Amino acid modifications
	Glycosylation	217	1	N-linked (GlcNAc...) Potential
	Glycosylation	218	1	N-linked (GlcNAc...) Potential
	Disulfide bond	41 ↔ 122		By similarity
	Disulfide bond	74 ↔ 79		By similarity
	Disulfide bond	128 ↔ 324		By similarity
	Disulfide bond	207 ↔ 234		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9FL16-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 2171431964F09048	FASTA	328	36,120
	10 20 30 40 50 60 MAEQSQLKNL TIILLLLCLS FQSLSFAAES HLTVDFYSKS CPKFLDIIRE TITNKQISTP 70 80 90 100 110 120 TTAAAALRLF FHDCFPNGCD ASVLVSSTAF NTAERDSSIN LSLPGDGFDV VIRAKTALEL 130 140 150 160 170 180 ACPNTVSCSD IIAVAVRDLL VTVGGPYYEI SLGRRDSRTS KSSLVSDLLP LPSMQISKLI 190 200 210 220 230 240 DQFSSRGFSV QEMVALSGAH TIGFSHCKEF TNRVNPNNST GYNPRFAVAL KKACSNSKND 250 260 270 280 290 300 PTISVFNDVM TPNKFDNMYF QNIPKGLGLL ESDHGLFSDP RTRPFVELYA RDQSRFFNDF 310 320 AGAMQKLSLH GVLTGRRGEI RRRCDAIN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones." Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S. DNA Res. 5:131-145(1998) [PubMed: 9679202] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed: 11910074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"From expressed sequence tags to structure, function, evolution and expression of 28 ER-targeted Arabidopsis peroxidases." Welinder K.G., Jespersen H.M., Kjaersgaard I.V.H., Justesen A.F., Oestergaard L., Abelskov A.K., Jensen R.B., Hansen L.N., Rasmussen S.K. Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 53-328. Strain: cv. Columbia.
[4]	"Analysis and expression of the class III peroxidase large gene family in Arabidopsis thaliana." Tognolli M., Penel C., Greppin H., Simon P. Gene 288:129-138(2002) [PubMed: 12034502] [Abstract] Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB010699 Genomic DNA. Translation: BAB10896.1. AK118632 mRNA. Translation: BAC43229.1. Y11791 mRNA. Translation: CAA72487.1.
IPI	IPI00535868.
RefSeq	NP_198831.1.
UniGene	At.88
3D structure databases
HSSP	HSSP built from PDB template 1QGJ based on UniProtKB Q39034.
ModBase	Search...
Protein family/group databases
PeroxiBase	229. AtPrx63.
Proteomic databases
PRIDE	Q9FL16.
Genome annotation databases
GeneID	834012.
GenomeReviews	Gene locus AT5G40150 in contig BA000015_GR.
KEGG	ath:AT5G40150.
NMPDR	fig|3702.1.peg.25714.
Organism-specific databases
GeneFarm	1922. 61.
TAIR	At5g40150.
Enzyme and pathway databases
BRENDA	1.11.1.7. 302.
Gene expression databases
ArrayExpress	Q9FL16.
GermOnline	AT5G40150. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002016. Haem_peroxidase_pln/fun/bac. IPR000823. Peroxidase_pln. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 1 hit. [Graphical view]
PRINTS	PR00458. PEROXIDASE. PR00461. PLPEROXIDASE.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PER63_ARATH

Accession

Primary (citable) accession number: Q9FL16
Secondary accession number(s): P93726

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2002
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)