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Protein

Ferredoxin--NADP reductase, leaf isozyme 1, chloroplastic

Gene

LFNR1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a key role in regulating the relative amounts of cyclic and non-cyclic electron flow to meet the demands of the plant for ATP and reducing power.2 Publications

Catalytic activityi

2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH.

Cofactori

Kineticsi

  1. KM=3.5 µM for ferredoxin-11 Publication
  2. KM=2.5 µM for ferredoxin-21 Publication
  3. KM=4.6 µM for ferredoxin-31 Publication

    Pathwayi: photosynthesis

    This protein is involved in the pathway photosynthesis, which is part of Energy metabolism.
    View all proteins of this organism that are known to be involved in the pathway photosynthesis and in Energy metabolism.

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi212 – 230NADPBy similarityAdd BLAST19

    GO - Molecular functioni

    GO - Biological processi

    • defense response to bacterium Source: TAIR
    • photosynthetic electron transport chain Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Photosynthesis, Transport

    Keywords - Ligandi

    FAD, Flavoprotein, NADP

    Enzyme and pathway databases

    BioCyciARA:AT5G66190-MONOMER.
    BRENDAi1.18.1.2. 399.
    UniPathwayiUPA00091.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ferredoxin--NADP reductase, leaf isozyme 1, chloroplastic (EC:1.18.1.2)
    Alternative name(s):
    Leaf FNR 1
    Short name:
    AtLFNR1
    Short name:
    FNR-1
    Gene namesi
    Name:LFNR1
    Synonyms:PETH1
    Ordered Locus Names:At5g66190
    ORF Names:K2A18.27
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    TAIRiAT5G66190.

    Subcellular locationi

    • Plastidchloroplast stroma 1 Publication
    • Plastidchloroplast thylakoid membrane 1 Publication; Peripheral membrane protein 1 Publication; Stromal side 1 Publication

    • Note: More abundant in the membrane fraction.

    GO - Cellular componenti

    • apoplast Source: TAIR
    • chloroplast Source: TAIR
    • chloroplast envelope Source: TAIR
    • chloroplast stroma Source: TAIR
    • chloroplast thylakoid Source: TAIR
    • chloroplast thylakoid membrane Source: TAIR
    • thylakoid Source: TAIR
    • thylakoid lumen Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Chloroplast, Membrane, Plastid, Thylakoid

    Pathology & Biotechi

    Disruption phenotypei

    Plants have a reduced capacity for carbon fixation and prevent the association of LFNR2 with the thylakoid membrane.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 49Chloroplast1 PublicationAdd BLAST49
    ChainiPRO_000032257250 – 360Ferredoxin--NADP reductase, leaf isozyme 1, chloroplasticAdd BLAST311

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi178 ↔ 183By similarity
    Modified residuei179PhosphoserineCombined sources1
    Modified residuei210PhosphothreonineCombined sources1

    Keywords - PTMi

    Disulfide bond, Phosphoprotein

    Proteomic databases

    PaxDbiQ9FKW6.
    PRIDEiQ9FKW6.

    PTM databases

    iPTMnetiQ9FKW6.

    Expressioni

    Tissue specificityi

    Expressed in shoots. Restricted to green tissues, being more abundant in siliques.1 Publication

    Inductioni

    By nitrate.1 Publication

    Gene expression databases

    ExpressionAtlasiQ9FKW6. baseline and differential.
    GenevisibleiQ9FKW6. AT.

    Interactioni

    Subunit structurei

    Heterodimer with LFNR2. Interacts with PGRL1A and PGRL1B. Interacts with TIC62.4 Publications

    Protein-protein interaction databases

    BioGridi21993. 6 interactors.
    IntActiQ9FKW6. 1 interactor.
    STRINGi3702.AT5G66190.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FKW6.
    SMRiQ9FKW6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini81 – 203FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST123

    Sequence similaritiesi

    Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiKOG1158. Eukaryota.
    COG0369. LUCA.
    HOGENOMiHOG000220125.
    InParanoidiQ9FKW6.
    KOiK02641.
    OMAiGRMYIQD.
    OrthoDBiEOG09360EAZ.
    PhylomeDBiQ9FKW6.

    Family and domain databases

    InterProiIPR017927. Fd_Rdtase_FAD-bd.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR015701. FNR.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF00175. NAD_binding_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000361. Frd-NADP+_RD. 1 hit.
    PRINTSiPR00371. FPNCR.
    SUPFAMiSSF63380. SSF63380. 1 hit.
    PROSITEiPS51384. FAD_FR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

    Note: A number of isoforms are produced. According to EST sequences.
    Isoform 1 (identifier: Q9FKW6-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MAAAISAAVS LPSSKSSSLL TKISSVSPQR IFLKKSTVCY RRVVSVKAQV
    60 70 80 90 100
    TTDTTEAPPV KVVKESKKQE EGIVVNKFKP KNPYTGRCLL NTKITGDDAP
    110 120 130 140 150
    GETWHIVFTT EGEVPYREGQ SIGVIPEGID KNGKPHKLRL YSIASSAIGD
    160 170 180 190 200
    FGDSKTVSLC VKRLVYTNDG GEIVKGVCSN FLCDLKPGDE AKITGPVGKE
    210 220 230 240 250
    MLMPKDPNAT IIMLGTGTGI APFRSFLWKM FFEEHEDYKF NGLAWLFLGV
    260 270 280 290 300
    PTSSSLLYKE EFEKMKEKNP DNFRLDFAVS REQTNEKGEK MYIQTRMAEY
    310 320 330 340 350
    AEELWELLKK DNTFVYMCGL KGMEKGIDDI MVSLAAKDGI DWLEYKKQLK
    360
    RSEQWNVEVY
    Length:360
    Mass (Da):40,326
    Last modified:March 1, 2001 - v1
    Checksum:iF50B5C79476283ED
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti2A → S in BAE98556 (Ref. 5) Curated1
    Sequence conflicti113E → G in CAB52472 (Ref. 1) Curated1
    Sequence conflicti276 – 278DFA → GFS in CAB52472 (Ref. 1) Curated3
    Sequence conflicti292Y → F in CAB52472 (Ref. 1) Curated1
    Sequence conflicti345Y → F in CAB52472 (Ref. 1) Curated1
    Sequence conflicti360Y → F in CAB52472 (Ref. 1) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ243705 mRNA. Translation: CAB52472.1.
    AB011474 Genomic DNA. Translation: BAB10424.1.
    CP002688 Genomic DNA. Translation: AED98174.1.
    AY072112 mRNA. Translation: AAL59934.1.
    AY096665 mRNA. Translation: AAM20299.1.
    AK226411 mRNA. Translation: BAE98556.1.
    RefSeqiNP_201420.1. NM_126017.5. [Q9FKW6-1]
    UniGeneiAt.47570.
    At.49233.

    Genome annotation databases

    EnsemblPlantsiAT5G66190.1; AT5G66190.1; AT5G66190. [Q9FKW6-1]
    GeneIDi836751.
    GrameneiAT5G66190.1; AT5G66190.1; AT5G66190.
    KEGGiath:AT5G66190.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ243705 mRNA. Translation: CAB52472.1.
    AB011474 Genomic DNA. Translation: BAB10424.1.
    CP002688 Genomic DNA. Translation: AED98174.1.
    AY072112 mRNA. Translation: AAL59934.1.
    AY096665 mRNA. Translation: AAM20299.1.
    AK226411 mRNA. Translation: BAE98556.1.
    RefSeqiNP_201420.1. NM_126017.5. [Q9FKW6-1]
    UniGeneiAt.47570.
    At.49233.

    3D structure databases

    ProteinModelPortaliQ9FKW6.
    SMRiQ9FKW6.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi21993. 6 interactors.
    IntActiQ9FKW6. 1 interactor.
    STRINGi3702.AT5G66190.1.

    PTM databases

    iPTMnetiQ9FKW6.

    Proteomic databases

    PaxDbiQ9FKW6.
    PRIDEiQ9FKW6.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT5G66190.1; AT5G66190.1; AT5G66190. [Q9FKW6-1]
    GeneIDi836751.
    GrameneiAT5G66190.1; AT5G66190.1; AT5G66190.
    KEGGiath:AT5G66190.

    Organism-specific databases

    TAIRiAT5G66190.

    Phylogenomic databases

    eggNOGiKOG1158. Eukaryota.
    COG0369. LUCA.
    HOGENOMiHOG000220125.
    InParanoidiQ9FKW6.
    KOiK02641.
    OMAiGRMYIQD.
    OrthoDBiEOG09360EAZ.
    PhylomeDBiQ9FKW6.

    Enzyme and pathway databases

    UniPathwayiUPA00091.
    BioCyciARA:AT5G66190-MONOMER.
    BRENDAi1.18.1.2. 399.

    Miscellaneous databases

    PROiQ9FKW6.

    Gene expression databases

    ExpressionAtlasiQ9FKW6. baseline and differential.
    GenevisibleiQ9FKW6. AT.

    Family and domain databases

    InterProiIPR017927. Fd_Rdtase_FAD-bd.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR015701. FNR.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF00175. NAD_binding_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000361. Frd-NADP+_RD. 1 hit.
    PRINTSiPR00371. FPNCR.
    SUPFAMiSSF63380. SSF63380. 1 hit.
    PROSITEiPS51384. FAD_FR. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiFNRL1_ARATH
    AccessioniPrimary (citable) accession number: Q9FKW6
    Secondary accession number(s): Q0WWE2, Q9SUJ3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 26, 2008
    Last sequence update: March 1, 2001
    Last modified: November 2, 2016
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.