Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Calcium-dependent protein kinase 28

Gene

CPK28

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger (Probable). Acts as developmentally controlled regulator for coordinated stem elongation and vascular development. Acts as key component which contributes to the developmental switch that establises the transition from vegetative to reproductive growth (PubMed:23252373). Involved in pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) signaling. Interacts with and phosphorylates the kinase BIK1, a central rate-limiting kinase in PTI signaling. Facilitates BIK1 turnover and negatively regulates BIK1-mediated immune responses triggered by several PAMPs. Its kinase activity is necessary and sufficient for its function in PTI signaling (PubMed:25525792).1 Publication2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Enzyme regulationi

Activated by calcium (PubMed:23252373). Autophosphorylation plays an important role in the regulation of the kinase activity (PubMed:23252373).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei91ATPPROSITE-ProRule annotation1
Active sitei188Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi68 – 76ATPPROSITE-ProRule annotation9
Calcium bindingi378 – 3891PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi415 – 4262PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi457 – 4683PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi487 – 4984PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Growth regulation, Plant defense

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase 281 Publication (EC:2.7.11.11 Publication)
Gene namesi
Name:CPK281 Publication
Ordered Locus Names:At5g66210
ORF Names:K2A18.29
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G66210.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • nucleus Source: GO_Central
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Severe growth defect in shoot elongation. Severe growth defect of flowering stem.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi188D → A: Abolishes kinase activity. 1 Publication1
Mutagenesisi245S → L in mod1; abolishes kinase activity; when associated with V-295. 1 Publication1
Mutagenesisi295A → V in mod1; abolishes kinase activity; when associated with L-245. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCurated
ChainiPRO_00003633502 – 523Calcium-dependent protein kinase 28Add BLAST522

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Lipidationi4S-palmitoyl cysteineBy similarity1
Modified residuei228Phosphoserine1 Publication1
Modified residuei318Phosphoserine1 Publication1
Modified residuei495Phosphoserine1 Publication1
Modified residuei515PhosphoserineCombined sources1

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ9FKW4.
PRIDEiQ9FKW4.

PTM databases

iPTMnetiQ9FKW4.
SwissPalmiQ9FKW4.

Expressioni

Tissue specificityi

Expressed in vascular and meristematic tissues throughout plant development.1 Publication

Gene expression databases

ExpressionAtlasiQ9FKW4. baseline and differential.
GenevisibleiQ9FKW4. AT.

Interactioni

Subunit structurei

Interacts with BIK1.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi3702.AT5G66210.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FKW4.
SMRiQ9FKW4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini62 – 322Protein kinasePROSITE-ProRule annotationAdd BLAST261
Domaini365 – 401EF-hand 1PROSITE-ProRule annotationAdd BLAST37
Domaini402 – 437EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini438 – 479EF-hand 3PROSITE-ProRule annotationAdd BLAST42
Domaini480 – 509EF-hand 4PROSITE-ProRule annotationAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni328 – 358Autoinhibitory domainBy similarityAdd BLAST31

Domaini

There is 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (328-358) inactivates kinase activity under calcium-free conditions (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation
Contains 4 EF-hand domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
InParanoidiQ9FKW4.
KOiK13412.
OMAiCFSAIRV.
OrthoDBiEOG093605DQ.
PhylomeDBiQ9FKW4.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9FKW4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGVCFSAIRV TGASSSRRSS QTKSKAAPTP IDTKASTKRR TGSIPCGKRT
60 70 80 90 100
DFGYSKDFHD HYTIGKLLGH GQFGYTYVAI HRPNGDRVAV KRLDKSKMVL
110 120 130 140 150
PIAVEDVKRE VQILIALSGH ENVVQFHNAF EDDDYVYIVM ELCEGGELLD
160 170 180 190 200
RILSKKGNRY SEKDAAVVVR QMLKVAGECH LHGLVHRDMK PENFLFKSAQ
210 220 230 240 250
LDSPLKATDF GLSDFIKPGK RFHDIVGSAY YVAPEVLKRR SGPESDVWSI
260 270 280 290 300
GVITYILLCG RRPFWDRTED GIFKEVLRNK PDFSRKPWAT ISDSAKDFVK
310 320 330 340 350
KLLVKDPRAR LTAAQALSHA WVREGGNATD IPVDISVLNN LRQFVRYSRL
360 370 380 390 400
KQFALRALAS TLDEAEISDL RDQFDAIDVD KNGVISLEEM RQALAKDLPW
410 420 430 440 450
KLKDSRVAEI LEAIDSNTDG LVDFTEFVAA ALHVHQLEEH DSEKWQLRSR
460 470 480 490 500
AAFEKFDLDK DGYITPEELR MHTGLRGSID PLLDEADIDR DGKISLHEFR
510 520
RLLRTASISS QRAPSPAGHR NLR
Length:523
Mass (Da):58,972
Last modified:March 1, 2001 - v1
Checksum:iC4211CAA1F22619D
GO
Isoform 2 (identifier: Q9FKW4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     472-488: HTGLRGSIDPLLDEADI → AQQNKERKWSENPIDEI
     489-523: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:488
Mass (Da):55,254
Checksum:iFCA1D0D1CEF861A1
GO
Isoform 3 (identifier: Q9FKW4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     414-435: IDSNTDGLVDFTEFVAAALHVH → VVIFLFSIAIASLGVSEGDVVS
     436-523: Missing.

Note: May be due to an intron retention. No experimental confirmation available.
Show »
Length:435
Mass (Da):48,644
Checksum:i52E82FE60A7271F2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti34K → E in BAF01180 (Ref. 4) Curated1
Sequence conflicti183G → D in AAM63052 (Ref. 5) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_036292414 – 435IDSNT…ALHVH → VVIFLFSIAIASLGVSEGDV VS in isoform 3. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_036293436 – 523Missing in isoform 3. 1 PublicationAdd BLAST88
Alternative sequenceiVSP_036294472 – 488HTGLR…DEADI → AQQNKERKWSENPIDEI in isoform 2. CuratedAdd BLAST17
Alternative sequenceiVSP_036295489 – 523Missing in isoform 2. CuratedAdd BLAST35

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011474 Genomic DNA. Translation: BAB10426.1.
CP002688 Genomic DNA. Translation: AED98177.1.
CP002688 Genomic DNA. Translation: AED98178.1.
CP002688 Genomic DNA. Translation: AED98179.1.
CP002688 Genomic DNA. Translation: AED98180.1.
AY139991 mRNA. Translation: AAM98133.1.
BT010380 mRNA. Translation: AAQ56823.1.
AK227052 mRNA. Translation: BAE99112.1.
AK229317 mRNA. Translation: BAF01180.1.
AY085837 mRNA. Translation: AAM63052.1.
RefSeqiNP_001078806.1. NM_001085337.1. [Q9FKW4-2]
NP_001119508.1. NM_001126036.2. [Q9FKW4-3]
NP_201422.1. NM_126019.4. [Q9FKW4-1]
NP_851280.1. NM_180949.3. [Q9FKW4-1]
UniGeneiAt.27527.

Genome annotation databases

EnsemblPlantsiAT5G66210.1; AT5G66210.1; AT5G66210. [Q9FKW4-1]
AT5G66210.2; AT5G66210.2; AT5G66210. [Q9FKW4-1]
GeneIDi836753.
GrameneiAT5G66210.1; AT5G66210.1; AT5G66210.
AT5G66210.2; AT5G66210.2; AT5G66210.
KEGGiath:AT5G66210.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011474 Genomic DNA. Translation: BAB10426.1.
CP002688 Genomic DNA. Translation: AED98177.1.
CP002688 Genomic DNA. Translation: AED98178.1.
CP002688 Genomic DNA. Translation: AED98179.1.
CP002688 Genomic DNA. Translation: AED98180.1.
AY139991 mRNA. Translation: AAM98133.1.
BT010380 mRNA. Translation: AAQ56823.1.
AK227052 mRNA. Translation: BAE99112.1.
AK229317 mRNA. Translation: BAF01180.1.
AY085837 mRNA. Translation: AAM63052.1.
RefSeqiNP_001078806.1. NM_001085337.1. [Q9FKW4-2]
NP_001119508.1. NM_001126036.2. [Q9FKW4-3]
NP_201422.1. NM_126019.4. [Q9FKW4-1]
NP_851280.1. NM_180949.3. [Q9FKW4-1]
UniGeneiAt.27527.

3D structure databases

ProteinModelPortaliQ9FKW4.
SMRiQ9FKW4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G66210.1.

PTM databases

iPTMnetiQ9FKW4.
SwissPalmiQ9FKW4.

Proteomic databases

PaxDbiQ9FKW4.
PRIDEiQ9FKW4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G66210.1; AT5G66210.1; AT5G66210. [Q9FKW4-1]
AT5G66210.2; AT5G66210.2; AT5G66210. [Q9FKW4-1]
GeneIDi836753.
GrameneiAT5G66210.1; AT5G66210.1; AT5G66210.
AT5G66210.2; AT5G66210.2; AT5G66210.
KEGGiath:AT5G66210.

Organism-specific databases

TAIRiAT5G66210.

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
InParanoidiQ9FKW4.
KOiK13412.
OMAiCFSAIRV.
OrthoDBiEOG093605DQ.
PhylomeDBiQ9FKW4.

Miscellaneous databases

PROiQ9FKW4.

Gene expression databases

ExpressionAtlasiQ9FKW4. baseline and differential.
GenevisibleiQ9FKW4. AT.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDPKS_ARATH
AccessioniPrimary (citable) accession number: Q9FKW4
Secondary accession number(s): A8MQP5, Q0WNW9, Q8LDS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.