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Protein

Calcium-dependent protein kinase 28

Gene

CPK28

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger (Probable). Acts as developmentally controlled regulator for coordinated stem elongation and vascular development. Acts as key component which contributes to the developmental switch that establishes the transition from vegetative to reproductive growth (PubMed:23252373). Involved in pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) signaling. Interacts with and phosphorylates the kinase BIK1, a central rate-limiting kinase in PTI signaling. Facilitates BIK1 turnover and negatively regulates BIK1-mediated immune responses triggered by several PAMPs. Its kinase activity is necessary and sufficient for its function in PTI signaling (PubMed:25525792).1 Publication2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Enzyme regulationi

Activated by calcium (PubMed:23252373). Autophosphorylation plays an important role in the regulation of the kinase activity (PubMed:23252373).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei91ATPPROSITE-ProRule annotation1
Active sitei188Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi68 – 76ATPPROSITE-ProRule annotation9
Calcium bindingi378 – 3891PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi415 – 4262PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi457 – 4683PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi487 – 4984PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Kinase, Serine/threonine-protein kinase, Transferase
Biological processGrowth regulation, Plant defense
LigandATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-111932 CaMK IV-mediated phosphorylation of CREB
R-ATH-442745 Activation of CaMK IV
R-ATH-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-ATH-69473 G2/M DNA damage checkpoint

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase 281 Publication (EC:2.7.11.11 Publication)
Gene namesi
Name:CPK281 Publication
Ordered Locus Names:At5g66210
ORF Names:K2A18.29
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G66210
TAIRilocus:2156947 AT5G66210

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Severe growth defect in shoot elongation. Severe growth defect of flowering stem.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi188D → A: Abolishes kinase activity. 1 Publication1
Mutagenesisi245S → L in mod1; abolishes kinase activity; when associated with V-295. 1 Publication1
Mutagenesisi295A → V in mod1; abolishes kinase activity; when associated with L-245. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCurated
ChainiPRO_00003633502 – 523Calcium-dependent protein kinase 28Add BLAST522

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Lipidationi4S-palmitoyl cysteineBy similarity1
Modified residuei228Phosphoserine1 Publication1
Modified residuei318Phosphoserine1 Publication1
Modified residuei495Phosphoserine1 Publication1
Modified residuei515PhosphoserineCombined sources1

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ9FKW4
PRIDEiQ9FKW4

PTM databases

iPTMnetiQ9FKW4
SwissPalmiQ9FKW4

Expressioni

Tissue specificityi

Expressed in vascular and meristematic tissues throughout plant development.1 Publication

Gene expression databases

ExpressionAtlasiQ9FKW4 baseline and differential
GenevisibleiQ9FKW4 AT

Interactioni

Subunit structurei

Interacts with BIK1.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi3702.AT5G66210.1

Structurei

3D structure databases

ProteinModelPortaliQ9FKW4
SMRiQ9FKW4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini62 – 322Protein kinasePROSITE-ProRule annotationAdd BLAST261
Domaini365 – 401EF-hand 1PROSITE-ProRule annotationAdd BLAST37
Domaini402 – 437EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini438 – 479EF-hand 3PROSITE-ProRule annotationAdd BLAST42
Domaini480 – 509EF-hand 4PROSITE-ProRule annotationAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni328 – 358Autoinhibitory domainBy similarityAdd BLAST31

Domaini

There are 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (328-358) inactivates kinase activity under calcium-free conditions (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032 Eukaryota
ENOG410XRMJ LUCA
HOGENOMiHOG000233030
InParanoidiQ9FKW4
KOiK13412
OMAiLRMHTGM
OrthoDBiEOG093605DQ
PhylomeDBiQ9FKW4

Family and domain databases

InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF13499 EF-hand_7, 2 hits
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 4 hits
SM00220 S_TKc, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 4 hits
PS50222 EF_HAND_2, 4 hits
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9FKW4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGVCFSAIRV TGASSSRRSS QTKSKAAPTP IDTKASTKRR TGSIPCGKRT
60 70 80 90 100
DFGYSKDFHD HYTIGKLLGH GQFGYTYVAI HRPNGDRVAV KRLDKSKMVL
110 120 130 140 150
PIAVEDVKRE VQILIALSGH ENVVQFHNAF EDDDYVYIVM ELCEGGELLD
160 170 180 190 200
RILSKKGNRY SEKDAAVVVR QMLKVAGECH LHGLVHRDMK PENFLFKSAQ
210 220 230 240 250
LDSPLKATDF GLSDFIKPGK RFHDIVGSAY YVAPEVLKRR SGPESDVWSI
260 270 280 290 300
GVITYILLCG RRPFWDRTED GIFKEVLRNK PDFSRKPWAT ISDSAKDFVK
310 320 330 340 350
KLLVKDPRAR LTAAQALSHA WVREGGNATD IPVDISVLNN LRQFVRYSRL
360 370 380 390 400
KQFALRALAS TLDEAEISDL RDQFDAIDVD KNGVISLEEM RQALAKDLPW
410 420 430 440 450
KLKDSRVAEI LEAIDSNTDG LVDFTEFVAA ALHVHQLEEH DSEKWQLRSR
460 470 480 490 500
AAFEKFDLDK DGYITPEELR MHTGLRGSID PLLDEADIDR DGKISLHEFR
510 520
RLLRTASISS QRAPSPAGHR NLR
Length:523
Mass (Da):58,972
Last modified:March 1, 2001 - v1
Checksum:iC4211CAA1F22619D
GO
Isoform 2 (identifier: Q9FKW4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     472-488: HTGLRGSIDPLLDEADI → AQQNKERKWSENPIDEI
     489-523: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:488
Mass (Da):55,254
Checksum:iFCA1D0D1CEF861A1
GO
Isoform 3 (identifier: Q9FKW4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     414-435: IDSNTDGLVDFTEFVAAALHVH → VVIFLFSIAIASLGVSEGDVVS
     436-523: Missing.

Note: May be due to an intron retention. No experimental confirmation available.
Show »
Length:435
Mass (Da):48,644
Checksum:i52E82FE60A7271F2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti34K → E in BAF01180 (Ref. 4) Curated1
Sequence conflicti183G → D in AAM63052 (Ref. 5) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_036292414 – 435IDSNT…ALHVH → VVIFLFSIAIASLGVSEGDV VS in isoform 3. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_036293436 – 523Missing in isoform 3. 1 PublicationAdd BLAST88
Alternative sequenceiVSP_036294472 – 488HTGLR…DEADI → AQQNKERKWSENPIDEI in isoform 2. CuratedAdd BLAST17
Alternative sequenceiVSP_036295489 – 523Missing in isoform 2. CuratedAdd BLAST35

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011474 Genomic DNA Translation: BAB10426.1
CP002688 Genomic DNA Translation: AED98177.1
CP002688 Genomic DNA Translation: AED98178.1
CP002688 Genomic DNA Translation: AED98179.1
CP002688 Genomic DNA Translation: AED98180.1
CP002688 Genomic DNA Translation: ANM69530.1
AY139991 mRNA Translation: AAM98133.1
BT010380 mRNA Translation: AAQ56823.1
AK227052 mRNA Translation: BAE99112.1
AK229317 mRNA Translation: BAF01180.1
AY085837 mRNA Translation: AAM63052.1
RefSeqiNP_001078806.1, NM_001085337.1 [Q9FKW4-2]
NP_001119508.1, NM_001126036.2 [Q9FKW4-3]
NP_001331200.1, NM_001345752.1 [Q9FKW4-3]
NP_201422.1, NM_126019.4 [Q9FKW4-1]
NP_851280.1, NM_180949.3 [Q9FKW4-1]
UniGeneiAt.27527

Genome annotation databases

EnsemblPlantsiAT5G66210.1; AT5G66210.1; AT5G66210 [Q9FKW4-1]
AT5G66210.2; AT5G66210.2; AT5G66210 [Q9FKW4-1]
AT5G66210.3; AT5G66210.3; AT5G66210 [Q9FKW4-2]
AT5G66210.4; AT5G66210.4; AT5G66210 [Q9FKW4-3]
AT5G66210.6; AT5G66210.6; AT5G66210 [Q9FKW4-3]
GeneIDi836753
GrameneiAT5G66210.1; AT5G66210.1; AT5G66210 [Q9FKW4-1]
AT5G66210.2; AT5G66210.2; AT5G66210 [Q9FKW4-1]
AT5G66210.3; AT5G66210.3; AT5G66210 [Q9FKW4-2]
AT5G66210.4; AT5G66210.4; AT5G66210 [Q9FKW4-3]
AT5G66210.6; AT5G66210.6; AT5G66210 [Q9FKW4-3]
KEGGiath:AT5G66210

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCDPKS_ARATH
AccessioniPrimary (citable) accession number: Q9FKW4
Secondary accession number(s): A8MQP5, Q0WNW9, Q8LDS1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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