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Protein

Nicotianamine synthase 2

Gene

NAS2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Synthesizes nicotianamine, a polyamine which serves as a sensor for the physiological iron status within the plant, and/or might be involved in the transport of iron.

Catalytic activityi

3 S-adenosyl-L-methionine = 3 S-methyl-5'-thioadenosine + nicotianamine.

GO - Molecular functioni

  1. nicotianamine synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular response to ethylene stimulus Source: TAIR
  2. cellular response to iron ion Source: TAIR
  3. cellular response to nitric oxide Source: TAIR
  4. nicotianamine biosynthetic process Source: InterPro
  5. phloem transport Source: TAIR
  6. pollen development Source: TAIR
  7. pollen tube growth Source: TAIR
  8. response to zinc ion Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciARA:AT5G56080-MONOMER.
BRENDAi2.5.1.43. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotianamine synthase 2 (EC:2.5.1.43)
Alternative name(s):
S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-adenosyl-methionine 3-amino-3-carboxypropyltransferase 2
Short name:
AtNAS2
Gene namesi
Name:NAS2
Ordered Locus Names:At5g56080
ORF Names:MDA7.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G56080.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320Nicotianamine synthase 2PRO_0000212701Add
BLAST

Proteomic databases

PaxDbiQ9FKT9.
PRIDEiQ9FKT9.

Expressioni

Gene expression databases

GenevestigatoriQ9FKT9.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G56080.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9FKT9.
SMRiQ9FKT9. Positions 90-274.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0500.
HOGENOMiHOG000025525.
InParanoidiQ9FKT9.
KOiK05953.
OMAiMACENNL.
PhylomeDBiQ9FKT9.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR004298. Nicotian_synth.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF03059. NAS. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51142. NAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FKT9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MACENNLVVK QIMDLYNQIS NLESLKPSKN VDTLFRQLVS TCLPTDTNID
60 70 80 90 100
VTEIHDEKVK DMRSHLIKLC GEAEGYLEQH FSAILGSFED NPLNHLHIFP
110 120 130 140 150
YYNNYLKLGK LEFDLLSQHT THVPTKVAFI GSGPMPLTSI VLAKFHLPNT
160 170 180 190 200
TFHNFDIDSH ANTLASNLVS RDSDLSKRMI FHTTDVLNAK EGLDQYDVVF
210 220 230 240 250
LAALVGMDKE SKVKAIEHLE KHMAPGAVVM LRSAHGLRAF LYPIVDSCDL
260 270 280 290 300
KGFEVLTIYH PSDDVVNSVV IARKLGGSNG ARGSQIGRCV VMPCNCSKVH
310 320
AILNNRGMEK NLIEEYSAIE
Length:320
Mass (Da):35,679
Last modified:March 1, 2001 - v1
Checksum:i0F089819D3C05FCA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41E → Q in BAA74590 (Ref. 1) Curated
Sequence conflicti316 – 3161Y → F in BAA74590 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB021935 Genomic DNA. Translation: BAA74590.1.
AB011476 Genomic DNA. Translation: BAB09290.1.
CP002688 Genomic DNA. Translation: AED96719.1.
BT030013 mRNA. Translation: ABN04751.1.
RefSeqiNP_200419.1. NM_124990.1.
UniGeneiAt.49799.

Genome annotation databases

EnsemblPlantsiAT5G56080.1; AT5G56080.1; AT5G56080.
GeneIDi835707.
KEGGiath:AT5G56080.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB021935 Genomic DNA. Translation: BAA74590.1.
AB011476 Genomic DNA. Translation: BAB09290.1.
CP002688 Genomic DNA. Translation: AED96719.1.
BT030013 mRNA. Translation: ABN04751.1.
RefSeqiNP_200419.1. NM_124990.1.
UniGeneiAt.49799.

3D structure databases

ProteinModelPortaliQ9FKT9.
SMRiQ9FKT9. Positions 90-274.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G56080.1-P.

Proteomic databases

PaxDbiQ9FKT9.
PRIDEiQ9FKT9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G56080.1; AT5G56080.1; AT5G56080.
GeneIDi835707.
KEGGiath:AT5G56080.

Organism-specific databases

TAIRiAT5G56080.

Phylogenomic databases

eggNOGiCOG0500.
HOGENOMiHOG000025525.
InParanoidiQ9FKT9.
KOiK05953.
OMAiMACENNL.
PhylomeDBiQ9FKT9.

Enzyme and pathway databases

BioCyciARA:AT5G56080-MONOMER.
BRENDAi2.5.1.43. 399.

Miscellaneous databases

PROiQ9FKT9.

Gene expression databases

GenevestigatoriQ9FKT9.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR004298. Nicotian_synth.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF03059. NAS. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51142. NAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of nicotianamine synthase genes from Arabidopsis thaliana."
    Suzuki K., Higuchi K., Nakanishi H., Nishizawa N.-K., Mori S.
    Soil Sci. Plant Nutr. 45:993-1002(1999)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones."
    Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R.
    Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiNAS2_ARATH
AccessioniPrimary (citable) accession number: Q9FKT9
Secondary accession number(s): A2RVM2, Q9ZWF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 13, 2002
Last sequence update: March 1, 2001
Last modified: April 29, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.