Q9FKT9 (NAS2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nicotianamine synthase 2
EC=2.5.1.43

Alternative name(s):
S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-adenosyl-methionine 3-amino-3-carboxypropyltransferase 2
Short name=AtNAS2

Gene names

Name:	NAS2
Ordered Locus Names:	At5g56080
ORF Names:	MDA7.14

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Synthesizes nicotianamine, a polyamine which serves as a sensor for the physiological iron status within the plant, and/or might be involved in the transport of iron.
Catalytic activity	3 S-adenosyl-L-methionine = 3 S-methyl-5'-thioadenosine + nicotianamine.
Sequence similarities	Belongs to the nicotianamine synthase (NAS)-like family.

Ontologies

Keywords
Ligand	S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to ethylene stimulus Inferred from expression pattern PubMed 20627899. Source: TAIR cellular response to iron ion Inferred from expression pattern PubMed 20627899. Source: TAIR cellular response to nitric oxide Inferred from expression pattern PubMed 20627899. Source: TAIR iron ion transport Inferred from electronic annotation. Source: EnsemblPlants/Gramene nicotianamine biosynthetic process Inferred from electronic annotation. Source: InterPro phloem transport Inferred from genetic interaction PubMed 22706286. Source: TAIR pollen development Inferred from genetic interaction PubMed 22706286. Source: TAIR pollen tube growth Inferred from genetic interaction PubMed 22706286. Source: TAIR response to zinc ion Inferred from expression pattern PubMed 14690510. Source: TAIR
Molecular_function	nicotianamine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Nicotianamine synthase 2		PRO_0000212701
Experimental info
	Sequence conflict	4	1	E → Q in BAA74590. Ref.1
	Sequence conflict	316	1	Y → F in BAA74590. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9FKT9 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 0F089819D3C05FCA	FASTA	320	35,679
	10 20 30 40 50 60 MACENNLVVK QIMDLYNQIS NLESLKPSKN VDTLFRQLVS TCLPTDTNID VTEIHDEKVK 70 80 90 100 110 120 DMRSHLIKLC GEAEGYLEQH FSAILGSFED NPLNHLHIFP YYNNYLKLGK LEFDLLSQHT 130 140 150 160 170 180 THVPTKVAFI GSGPMPLTSI VLAKFHLPNT TFHNFDIDSH ANTLASNLVS RDSDLSKRMI 190 200 210 220 230 240 FHTTDVLNAK EGLDQYDVVF LAALVGMDKE SKVKAIEHLE KHMAPGAVVM LRSAHGLRAF 250 260 270 280 290 300 LYPIVDSCDL KGFEVLTIYH PSDDVVNSVV IARKLGGSNG ARGSQIGRCV VMPCNCSKVH 310 320 AILNNRGMEK NLIEEYSAIE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of nicotianamine synthase genes from Arabidopsis thaliana." Suzuki K., Higuchi K., Nakanishi H., Nishizawa N.-K., Mori S. Soil Sci. Plant Nutr. 45:993-1002(1999) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones." Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S. DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB021935 Genomic DNA. Translation: BAA74590.1. AB011476 Genomic DNA. Translation: BAB09290.1. CP002688 Genomic DNA. Translation: AED96719.1. BT030013 mRNA. Translation: ABN04751.1.
IPI	IPI00531990.
RefSeq	NP_200419.1. NM_124990.1.
UniGene	At.49799.
3D structure databases
ProteinModelPortal	Q9FKT9.
SMR	Q9FKT9. Positions 90-274.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT5G56080.1-P.
Proteomic databases
PaxDb	Q9FKT9.
PRIDE	Q9FKT9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G56080.1; AT5G56080.1; AT5G56080.
GeneID	835707.
KEGG	ath:AT5G56080.
Organism-specific databases
TAIR	At5g56080.
Phylogenomic databases
eggNOG	COG0500.
HOGENOM	HOG000025525.
InParanoid	Q9FKT9.
KO	K05953.
OMA	MACENNL.
PhylomeDB	Q9FKT9.
ProtClustDB	PLN03075.
Gene expression databases
Genevestigator	Q9FKT9.
GermOnline	AT5G56080. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR004298. Nicotian_synth. [Graphical view]
Pfam	PF03059. NAS. 1 hit. [Graphical view]
PROSITE	PS51142. NAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAS2_ARATH

Accession

Primary (citable) accession number: Q9FKT9
Secondary accession number(s): A2RVM2, Q9ZWF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 13, 2002
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program