Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin-conjugating enzyme E2 30

Gene

UBC30

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei85Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ubiquitin-protein transferase activity Source: TAIR

GO - Biological processi

  • ubiquitin-dependent protein catabolic process Source: TAIR

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-5689896. Ovarian tumor domain proteases.
R-ATH-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
R-ATH-8866654. E3 ubiquitin ligases ubiquitinate target proteins.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 30 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 30
Ubiquitin carrier protein 30
Gene namesi
Name:UBC30
Ordered Locus Names:At5g56150
ORF Names:MDA7.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G56150.
TAIRilocus:2161850. AT5G56150.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003451951 – 148Ubiquitin-conjugating enzyme E2 30Add BLAST148

Proteomic databases

PaxDbiQ9FKT3.

Expressioni

Tissue specificityi

Ubiquitously expressed at very low levels.1 Publication

Gene expression databases

ExpressionAtlasiQ9FKT3. baseline and differential.
GenevisibleiQ9FKT3. AT.

Interactioni

Subunit structurei

Interacts with RGLG3 and RGLG4.1 Publication

Protein-protein interaction databases

BioGridi20958. 4 interactors.
STRINGi3702.AT5G56150.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FKT3.
SMRiQ9FKT3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0417. Eukaryota.
COG5078. LUCA.
HOGENOMiHOG000233455.
InParanoidiQ9FKT3.
KOiK06689.
OMAiNTHAREW.
OrthoDBiEOG09360PU6.
PhylomeDBiQ9FKT3.

Family and domain databases

CDDicd00195. UBCc. 1 hit.
Gene3Di3.10.110.10. 1 hit.
InterProiView protein in InterPro
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
PfamiView protein in Pfam
PF00179. UQ_con. 1 hit.
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiView protein in PROSITE
PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9FKT3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASKRINKEL RDLQRDPPVS CSAGPTGDDM FQWQATIMGP ADSPFAGGVF
60 70 80 90 100
LVTIHFPPDY PFKPPKVAFR TKVYHPNINS NGSICLDILK EQWSPALTVS
110 120 130 140
KVLLSICSLL TDPNPDDPLV PEIAHIYKTD RVKYESTAQS WTQKYAMG
Length:148
Mass (Da):16,480
Last modified:March 1, 2001 - v1
Checksum:i3FAF79744E8821C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ027043 mRNA. Translation: AAY44869.1.
AB011476 Genomic DNA. Translation: BAB09297.1.
CP002688 Genomic DNA. Translation: AED96726.1.
CP002688 Genomic DNA. Translation: AED96727.1.
AY059806 mRNA. Translation: AAL24288.1.
AY081511 mRNA. Translation: AAM10073.1.
AY084220 mRNA. Translation: AAM60821.1.
RefSeqiNP_001318808.1. NM_001345178.1.
NP_851198.1. NM_180867.3.
UniGeneiAt.24651.

Genome annotation databases

EnsemblPlantsiAT5G56150.1; AT5G56150.1; AT5G56150.
GeneIDi835714.
GrameneiAT5G56150.1; AT5G56150.1; AT5G56150.
KEGGiath:AT5G56150.

Similar proteinsi

Entry informationi

Entry nameiUBC30_ARATH
AccessioniPrimary (citable) accession number: Q9FKT3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: March 1, 2001
Last modified: August 30, 2017
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families