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Protein

Ubiquitin-conjugating enzyme E2 30

Gene

UBC30

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei85Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ubiquitin-protein transferase activity Source: TAIR

GO - Biological processi

  • ubiquitin-dependent protein catabolic process Source: TAIR

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-ATH-5689896 Ovarian tumor domain proteases
R-ATH-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-ATH-8866654 E3 ubiquitin ligases ubiquitinate target proteins
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 30 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 30
Ubiquitin carrier protein 30
Gene namesi
Name:UBC30
Ordered Locus Names:At5g56150
ORF Names:MDA7.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G56150
TAIRilocus:2161850 AT5G56150

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003451951 – 148Ubiquitin-conjugating enzyme E2 30Add BLAST148

Proteomic databases

PaxDbiQ9FKT3

Expressioni

Tissue specificityi

Ubiquitously expressed at very low levels.1 Publication

Gene expression databases

ExpressionAtlasiQ9FKT3 baseline and differential
GenevisibleiQ9FKT3 AT

Interactioni

Subunit structurei

Interacts with RGLG3 and RGLG4.1 Publication

Protein-protein interaction databases

BioGridi20958, 4 interactors
STRINGi3702.AT5G56150.1

Structurei

3D structure databases

ProteinModelPortaliQ9FKT3
SMRiQ9FKT3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0417 Eukaryota
COG5078 LUCA
HOGENOMiHOG000233455
InParanoidiQ9FKT3
KOiK06689
OMAiEDFNTIF
OrthoDBiEOG09360PU6
PhylomeDBiQ9FKT3

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9FKT3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASKRINKEL RDLQRDPPVS CSAGPTGDDM FQWQATIMGP ADSPFAGGVF
60 70 80 90 100
LVTIHFPPDY PFKPPKVAFR TKVYHPNINS NGSICLDILK EQWSPALTVS
110 120 130 140
KVLLSICSLL TDPNPDDPLV PEIAHIYKTD RVKYESTAQS WTQKYAMG
Length:148
Mass (Da):16,480
Last modified:March 1, 2001 - v1
Checksum:i3FAF79744E8821C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ027043 mRNA Translation: AAY44869.1
AB011476 Genomic DNA Translation: BAB09297.1
CP002688 Genomic DNA Translation: AED96726.1
AY059806 mRNA Translation: AAL24288.1
AY081511 mRNA Translation: AAM10073.1
AY084220 mRNA Translation: AAM60821.1
RefSeqiNP_851198.1, NM_180867.3
UniGeneiAt.24651

Genome annotation databases

EnsemblPlantsiAT5G56150.1; AT5G56150.1; AT5G56150
GeneIDi835714
GrameneiAT5G56150.1; AT5G56150.1; AT5G56150
KEGGiath:AT5G56150

Similar proteinsi

Entry informationi

Entry nameiUBC30_ARATH
AccessioniPrimary (citable) accession number: Q9FKT3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: March 1, 2001
Last modified: April 25, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health