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Q9FKT3

- UBC30_ARATH

UniProt

Q9FKT3 - UBC30_ARATH

Protein

Ubiquitin-conjugating enzyme E2 30

Gene

UBC30

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
  1. Functioni

    Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei85 – 851Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. ubiquitin-protein transferase activity Source: TAIR

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: TAIR

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G56150-MONOMER.
    ARA:GQT-378-MONOMER.
    ReactomeiREACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_185297. Separation of Sister Chromatids.
    REACT_185305. APC/C:Cdc20 mediated degradation of Cyclin B.
    REACT_187954. Senescence-Associated Secretory Phenotype (SASP).
    REACT_208398. Inactivation of APC/C via direct inhibition of the APC/C complex.
    REACT_94347. APC/C:Cdc20 mediated degradation of Securin.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 30 (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein 30
    Gene namesi
    Name:UBC30
    Ordered Locus Names:At5g56150
    ORF Names:MDA7.21
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G56150.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 148148Ubiquitin-conjugating enzyme E2 30PRO_0000345195Add
    BLAST

    Proteomic databases

    PaxDbiQ9FKT3.
    PRIDEiQ9FKT3.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed at very low levels.1 Publication

    Gene expression databases

    GenevestigatoriQ9FKT3.

    Interactioni

    Protein-protein interaction databases

    BioGridi20958. 2 interactions.
    STRINGi3702.AT5G56150.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FKT3.
    SMRiQ9FKT3. Positions 1-147.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    HOGENOMiHOG000233455.
    InParanoidiQ9FKT3.
    KOiK06689.
    OMAiIYKTDRV.
    PhylomeDBiQ9FKT3.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9FKT3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASKRINKEL RDLQRDPPVS CSAGPTGDDM FQWQATIMGP ADSPFAGGVF    50
    LVTIHFPPDY PFKPPKVAFR TKVYHPNINS NGSICLDILK EQWSPALTVS 100
    KVLLSICSLL TDPNPDDPLV PEIAHIYKTD RVKYESTAQS WTQKYAMG 148
    Length:148
    Mass (Da):16,480
    Last modified:March 1, 2001 - v1
    Checksum:i3FAF79744E8821C7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ027043 mRNA. Translation: AAY44869.1.
    AB011476 Genomic DNA. Translation: BAB09297.1.
    CP002688 Genomic DNA. Translation: AED96726.1.
    CP002688 Genomic DNA. Translation: AED96727.1.
    AY059806 mRNA. Translation: AAL24288.1.
    AY081511 mRNA. Translation: AAM10073.1.
    AY084220 mRNA. Translation: AAM60821.1.
    RefSeqiNP_568835.1. NM_124997.3.
    NP_851198.1. NM_180867.2.
    UniGeneiAt.24651.

    Genome annotation databases

    EnsemblPlantsiAT5G56150.1; AT5G56150.1; AT5G56150.
    AT5G56150.2; AT5G56150.2; AT5G56150.
    GeneIDi835714.
    KEGGiath:AT5G56150.

    Cross-referencesi

    Web resourcesi

    PlantsUBQ

    A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ027043 mRNA. Translation: AAY44869.1 .
    AB011476 Genomic DNA. Translation: BAB09297.1 .
    CP002688 Genomic DNA. Translation: AED96726.1 .
    CP002688 Genomic DNA. Translation: AED96727.1 .
    AY059806 mRNA. Translation: AAL24288.1 .
    AY081511 mRNA. Translation: AAM10073.1 .
    AY084220 mRNA. Translation: AAM60821.1 .
    RefSeqi NP_568835.1. NM_124997.3.
    NP_851198.1. NM_180867.2.
    UniGenei At.24651.

    3D structure databases

    ProteinModelPortali Q9FKT3.
    SMRi Q9FKT3. Positions 1-147.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 20958. 2 interactions.
    STRINGi 3702.AT5G56150.1-P.

    Proteomic databases

    PaxDbi Q9FKT3.
    PRIDEi Q9FKT3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G56150.1 ; AT5G56150.1 ; AT5G56150 .
    AT5G56150.2 ; AT5G56150.2 ; AT5G56150 .
    GeneIDi 835714.
    KEGGi ath:AT5G56150.

    Organism-specific databases

    TAIRi AT5G56150.

    Phylogenomic databases

    eggNOGi COG5078.
    HOGENOMi HOG000233455.
    InParanoidi Q9FKT3.
    KOi K06689.
    OMAi IYKTDRV.
    PhylomeDBi Q9FKT3.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci ARA:AT5G56150-MONOMER.
    ARA:GQT-378-MONOMER.
    Reactomei REACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_185297. Separation of Sister Chromatids.
    REACT_185305. APC/C:Cdc20 mediated degradation of Cyclin B.
    REACT_187954. Senescence-Associated Secretory Phenotype (SASP).
    REACT_208398. Inactivation of APC/C via direct inhibition of the APC/C complex.
    REACT_94347. APC/C:Cdc20 mediated degradation of Securin.

    Gene expression databases

    Genevestigatori Q9FKT3.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
      Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
      Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones."
      Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S.
      DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiUBC30_ARATH
    AccessioniPrimary (citable) accession number: Q9FKT3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 22, 2008
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3