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Protein

Cytochrome c1 2, heme protein, mitochondrial

Gene

CYC12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei103Heme (covalent)PROSITE-ProRule annotation1
Binding sitei106Heme (covalent)PROSITE-ProRule annotation1
Metal bindingi107Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi226Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c1 2, heme protein, mitochondrial
Alternative name(s):
Complex III subunit 4-2
Complex III subunit IV-2
Cytochrome b-c1 complex subunit 4-2
Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit 2
Short name:
Cytochrome c-1 2
Gene namesi
Name:CYC12
Ordered Locus Names:At5g40810
ORF Names:MHK7.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G40810.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei268 – 288Helical; Note=Anchors to the membraneSequence analysisAdd BLAST21

GO - Cellular componenti

  • membrane Source: TAIR
  • mitochondrial respiratory chain complex III Source: TAIR
  • mitochondrion Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 64MitochondrionSequence analysisAdd BLAST64
ChainiPRO_000042867365 – 307Cytochrome c1 2, heme protein, mitochondrialAdd BLAST243

Post-translational modificationi

Binds 1 heme group per subunit.By similarity

Proteomic databases

PaxDbiQ9FKS5.
PRIDEiQ9FKS5.

Expressioni

Gene expression databases

ExpressionAtlasiQ9FKS5. baseline and differential.
GenevisibleiQ9FKS5. AT.

Interactioni

Subunit structurei

The main subunits of complex b-c1 are: cytochrome b, cytochrome c1 and the Rieske protein. Associated to the mitochondrial respiratory chain complex III.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CCMFN2Q338844EBI-1777995,EBI-763400
CYCSP999992EBI-1777995,EBI-446479From a different organism.

Protein-protein interaction databases

BioGridi19332. 1 interactor.
IntActiQ9FKS5. 4 interactors.
MINTiMINT-4330364.
STRINGi3702.AT5G40810.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FKS5.
SMRiQ9FKS5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini90 – 197Cytochrome cPROSITE-ProRule annotationAdd BLAST108

Sequence similaritiesi

Belongs to the cytochrome c family.Curated
Contains 1 cytochrome c domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3052. Eukaryota.
COG2857. LUCA.
HOGENOMiHOG000003867.
InParanoidiQ9FKS5.
KOiK00413.
OMAiKMLLMMG.
OrthoDBiEOG09360JHE.
PhylomeDBiQ9FKS5.

Family and domain databases

Gene3Di1.10.760.10. 1 hit.
InterProiIPR009056. Cyt_c-like_dom.
IPR002326. Cyt_c1.
IPR021157. Cyt_c1_TM_anchor_C.
[Graphical view]
PANTHERiPTHR10266. PTHR10266. 1 hit.
PfamiPF02167. Cytochrom_C1. 1 hit.
[Graphical view]
PRINTSiPR00603. CYTOCHROMEC1.
SUPFAMiSSF46626. SSF46626. 1 hit.
SSF81496. SSF81496. 1 hit.
PROSITEiPS51007. CYTC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9FKS5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVGGGVIRQL LRRKLHSQSV ATPVLSWLSS KKANEDAGSA GLRAFALMGA
60 70 80 90 100
GITGLLSFST VASADEAEHG LECPNYPWPH EGILSSYDHA SIRRGHQVYQ
110 120 130 140 150
QVCASCHSMS LISYRDLVGV AYTEEEAKAM AAEIEVVDGP NDEGEMFTRP
160 170 180 190 200
GKLSDRLPEP YSNESAARFA NGGAYPPDLS LVTKARHNGQ NYVFALLTGY
210 220 230 240 250
RDPPAGISIR EGLHYNPYFP GGAIAMPKML NDEAVEYEDG TPATEAQMGK
260 270 280 290 300
DVVSFLSWAA EPEMEERKLM GFKWIFLLSL ALLQAAYYRR LKWSVLKSRK

LVLDVVN
Length:307
Mass (Da):33,690
Last modified:March 1, 2001 - v1
Checksum:i3F807D081F788ED3
GO
Isoform 2 (identifier: Q9FKS5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-47: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:260
Mass (Da):28,730
Checksum:i3E4ABFA491DD055D
GO

Sequence cautioni

The sequence CAA81123 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti83 – 87ILSSY → HFSSS in CAA81123 (Ref. 5) Curated5
Sequence conflicti141N → H in BAF01305 (Ref. 4) Curated1
Sequence conflicti144G → C in AAK91357 (PubMed:14593172).Curated1
Sequence conflicti144G → C in AAM51571 (PubMed:14593172).Curated1
Sequence conflicti162S → L in AAK91357 (PubMed:14593172).Curated1
Sequence conflicti162S → L in AAM51571 (PubMed:14593172).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0541781 – 47Missing in isoform 2. CuratedAdd BLAST47

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011477 Genomic DNA. Translation: BAB11343.1.
CP002688 Genomic DNA. Translation: AED94597.1.
CP002688 Genomic DNA. Translation: AED94598.1.
AY050340 mRNA. Translation: AAK91357.1.
AY116937 mRNA. Translation: AAM51571.1.
AK229445 mRNA. Translation: BAF01305.1.
AK230342 mRNA. Translation: BAF02141.1.
Z25972 mRNA. Translation: CAA81123.1. Different initiation.
RefSeqiNP_001154756.1. NM_001161284.1. [Q9FKS5-2]
NP_198897.1. NM_123446.5. [Q9FKS5-1]
UniGeneiAt.23244.
At.75407.

Genome annotation databases

EnsemblPlantsiAT5G40810.1; AT5G40810.1; AT5G40810. [Q9FKS5-1]
AT5G40810.2; AT5G40810.2; AT5G40810. [Q9FKS5-2]
GeneIDi834081.
GrameneiAT5G40810.1; AT5G40810.1; AT5G40810.
AT5G40810.2; AT5G40810.2; AT5G40810.
KEGGiath:AT5G40810.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011477 Genomic DNA. Translation: BAB11343.1.
CP002688 Genomic DNA. Translation: AED94597.1.
CP002688 Genomic DNA. Translation: AED94598.1.
AY050340 mRNA. Translation: AAK91357.1.
AY116937 mRNA. Translation: AAM51571.1.
AK229445 mRNA. Translation: BAF01305.1.
AK230342 mRNA. Translation: BAF02141.1.
Z25972 mRNA. Translation: CAA81123.1. Different initiation.
RefSeqiNP_001154756.1. NM_001161284.1. [Q9FKS5-2]
NP_198897.1. NM_123446.5. [Q9FKS5-1]
UniGeneiAt.23244.
At.75407.

3D structure databases

ProteinModelPortaliQ9FKS5.
SMRiQ9FKS5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19332. 1 interactor.
IntActiQ9FKS5. 4 interactors.
MINTiMINT-4330364.
STRINGi3702.AT5G40810.1.

Proteomic databases

PaxDbiQ9FKS5.
PRIDEiQ9FKS5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G40810.1; AT5G40810.1; AT5G40810. [Q9FKS5-1]
AT5G40810.2; AT5G40810.2; AT5G40810. [Q9FKS5-2]
GeneIDi834081.
GrameneiAT5G40810.1; AT5G40810.1; AT5G40810.
AT5G40810.2; AT5G40810.2; AT5G40810.
KEGGiath:AT5G40810.

Organism-specific databases

TAIRiAT5G40810.

Phylogenomic databases

eggNOGiKOG3052. Eukaryota.
COG2857. LUCA.
HOGENOMiHOG000003867.
InParanoidiQ9FKS5.
KOiK00413.
OMAiKMLLMMG.
OrthoDBiEOG09360JHE.
PhylomeDBiQ9FKS5.

Miscellaneous databases

PROiQ9FKS5.

Gene expression databases

ExpressionAtlasiQ9FKS5. baseline and differential.
GenevisibleiQ9FKS5. AT.

Family and domain databases

Gene3Di1.10.760.10. 1 hit.
InterProiIPR009056. Cyt_c-like_dom.
IPR002326. Cyt_c1.
IPR021157. Cyt_c1_TM_anchor_C.
[Graphical view]
PANTHERiPTHR10266. PTHR10266. 1 hit.
PfamiPF02167. Cytochrom_C1. 1 hit.
[Graphical view]
PRINTSiPR00603. CYTOCHROMEC1.
SUPFAMiSSF46626. SSF46626. 1 hit.
SSF81496. SSF81496. 1 hit.
PROSITEiPS51007. CYTC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYC1B_ARATH
AccessioniPrimary (citable) accession number: Q9FKS5
Secondary accession number(s): F4KIR8
, Q0WL66, Q0WNJ4, Q42065, Q94A63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.