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Protein

Serine/threonine-protein kinase ATR

Gene

ATR

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Probable serine/threonine kinase. Plays a central role in cell-cycle regulation by transmitting DNA damage signals to downstream effectors of cell-cycle progression. May recognize the substrate consensus sequence [ST]-Q and phosphorylate histone variant H2AX to form H2AXS139ph at sites of DNA damage, thereby regulating DNA damage response mechanism. Seems to be required for the G2-phase checkpoint in response to replication blocks but not absolutely required in the G2-arrest response to double-strand breaks. May also be involved in the meiosis process. Required for the basal expression of RNR1 (ribonucleotide reductase large subunit). Acts in concert with telomerase to maintain telomeric DNA tracts. Not required for telomere length homeostasis.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

GO - Molecular functioni

GO - Biological processi

  • DNA repair Source: TAIR
  • double-strand break repair via nonhomologous end joining Source: TAIR
  • meiotic nuclear division Source: TAIR
  • multicellular organism reproduction Source: TAIR
  • regulation of chromosome organization Source: TAIR
  • regulation of telomere maintenance Source: TAIR
  • response to aluminum ion Source: TAIR
  • response to gamma radiation Source: TAIR
  • telomere maintenance in response to DNA damage Source: TAIR
  • telomere maintenance via telomerase Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G40820-MONOMER.
ReactomeiR-ATH-3371453. Regulation of HSF1-mediated heat shock response.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase ATR (EC:2.7.11.1)
Short name:
AtATR
Alternative name(s):
Ataxia telangiectasia-mutated and Rad3-related homolog
DNA repair protein ATR
Rad3-like protein
Short name:
AtRAD3
Gene namesi
Name:ATR
Synonyms:RAD3
Ordered Locus Names:At5g40820
ORF Names:MHK7.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G40820.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB-SubCell
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 27022702Serine/threonine-protein kinase ATRPRO_0000088838Add
BLAST

Proteomic databases

PaxDbiQ9FKS4.
PRIDEiQ9FKS4.

PTM databases

iPTMnetiQ9FKS4.

Expressioni

Inductioni

Strongly induced by replication blocking agents (UVB, aphidicolin and hydroxyurea) but only mildly induced by DNA damaging agents (gamma-radiation).1 Publication

Gene expression databases

GenevisibleiQ9FKS4. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G40820.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FKS4.
SMRiQ9FKS4. Positions 1653-2298.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1646 – 2255610FATPROSITE-ProRule annotationAdd
BLAST
Domaini2392 – 2638247PI3K/PI4KPROSITE-ProRule annotationAdd
BLAST
Domaini2670 – 270233FATCPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PI3/PI4-kinase family. ATM subfamily.Curated
Contains 1 FAT domain.PROSITE-ProRule annotation
Contains 1 FATC domain.PROSITE-ProRule annotation
Contains 1 PI3K/PI4K domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0890. Eukaryota.
COG5032. LUCA.
HOGENOMiHOG000030210.
InParanoidiQ9FKS4.
KOiK06640.
OMAiMRGCLKD.
PhylomeDBiQ9FKS4.

Family and domain databases

Gene3Di1.10.1070.11. 2 hits.
1.25.40.10. 2 hits.
InterProiIPR016024. ARM-type_fold.
IPR003152. FATC_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR003151. PIK-rel_kinase_FAT.
IPR014009. PIK_FAT.
IPR011990. TPR-like_helical_dom.
IPR012993. UME.
[Graphical view]
PfamiPF02259. FAT. 1 hit.
PF02260. FATC. 1 hit.
PF00454. PI3_PI4_kinase. 1 hit.
PF08064. UME. 1 hit.
[Graphical view]
SMARTiSM01343. FATC. 1 hit.
SM00146. PI3Kc. 1 hit.
SM00802. UME. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 5 hits.
SSF56112. SSF56112. 2 hits.
PROSITEiPS51189. FAT. 1 hit.
PS51190. FATC. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FKS4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKDDNNLSS LVHELRERVA ASASTPANNL RHSSGDEDAL EIRFRAVIPN
60 70 80 90 100
LLNTYVVPSL GNGREVTAVL KLVGHTARNI PGVFYHGTPS AILPVIARII
110 120 130 140 150
PFFAEPEFVP GHGVLLETVG SLLMLLRSNS RKAYRIFFHD ALQAIQDMQP
160 170 180 190 200
IASLHSIEPE VCESHIPFRC FCMSFSGIGG DLPDANKPRD GDGLVLNLLG
210 220 230 240 250
ANRWQPFATC ILKLICKCLT EGTLYVQGLI HTSFFKAACS LVCCGGADVQ
260 270 280 290 300
MACFEFATLV GSILTFNILP HVALIQSIIL LLSADEGLPV YRNTIYDSTI
310 320 330 340 350
GRFLTAVYSS CSDAAVKLTA ESLVLVLSHA LQRTKSEELK ASLCSAYVRI
360 370 380 390 400
VKSCPPCIWK IHCLLELLHL PEPCFQLIEC FKAVLIVLGP GCVRVETTKC
410 420 430 440 450
GSHTSATSDR PVQGINAGKK RHIEDESTYK RKRQKVGDDI RRGVYFAPEF
460 470 480 490 500
ADETDGKDAA SLREMLISTV ESLKPPPAGP SLSQTESSIV ALSMLTNAFC
510 520 530 540 550
FCPWTDMTHR LFNQMYAWIP WIAGQVEETN PIMFDISIYL EGIHNLLLVG
560 570 580 590 600
VDPQYEYTSK GNDLVAIQFL LKLPWTHYML FKTPSSLVKS KCLSVGIWTK
610 620 630 640 650
LGLQDGSDFD IFSWSLSDDF EQVQAVAAIS MPLKVLFSGL GALLHMFPKL
660 670 680 690 700
EHLLEEKELM IKKAIPQSLG FLSCLYGSST TDSEKTACHL LLHEDLKKDE
710 720 730 740 750
TLNSLLQGFR CSKCDKFIER EDEKHFRIIE TPEMVKLKMD HHRDYFNLQS
760 770 780 790 800
LYFNLLYDES SEETQLACVE VIRRILGHTS PDILVRTRSQ WIRCLQYLLV
810 820 830 840 850
HVNTDVREAF CAQIGIFVQH PIVSCLFLSE DATEKSCERN FFNLIEHSLA
860 870 880 890 900
AAKDLLVIQT LLETTAEVMV AVDVTSELFL ICLFLLIDQL DHPNLIVRIN
910 920 930 940 950
ASKLINRSCY IHVKGGFATL LSTASHIQNE LFDNLSVRLT SRPNVVREFA
960 970 980 990 1000
EAVLGVETEE LVRKMVPAVL PKLLVYWQEN AQAANTLNEL AKLIDTDVVP
1010 1020 1030 1040 1050
LIVNWLPRVL AFALNQEEDK NLLSVLQLYH SQIGSDNQEI FAAALPALLD
1060 1070 1080 1090 1100
ELVCFVDIAD TPETDRRLQR LPDAIKKISK VLTNAEDLPG FLQNHFVGLL
1110 1120 1130 1140 1150
NSIDRKMLHA DDIFLQKQAL KRIKLLIEMM GHYLSTYVPK LMVLLMHAIE
1160 1170 1180 1190 1200
KDALQSEGLL VLHFFTRKLA DVSPSSIKYV ISQIFAALIP FLEKEKEGPH
1210 1220 1230 1240 1250
VYLDEVVKIL EELVLKNRDI VKEHICEFPL LPSIPSLGEL NNAIQEARGL
1260 1270 1280 1290 1300
MSLKDQLRDI VNGMKHENLN VRYMVACELS KLLYNRNEDV AALIAGELVS
1310 1320 1330 1340 1350
DMEILSSLIT YLLQGCAEES RTTVGQRLKL VCADCLGAIG AIDPAKVRVA
1360 1370 1380 1390 1400
SCSRFKIQCS DDDLIFELIH KHLARAFRAA QDTIIQDSAA LAIQELLKIA
1410 1420 1430 1440 1450
GCEPSLAGNV VVLTPQEHVQ VNVSGSRRCG GNNEVKDRGQ KLWDRFSNYV
1460 1470 1480 1490 1500
KELIAPCLTS RFQLPNVSDP GSAGPIYRPS MSFRRWLSYW IRKLTAFATG
1510 1520 1530 1540 1550
SRVSIFAACR GIVRHDMQTA TYLLPYLVLD VVCHGTEAAR LSISEEILSV
1560 1570 1580 1590 1600
LDAAASENSG VTINSFGVGQ SEVCVQAVFT LLDNLGQWVD DVKQGVALSS
1610 1620 1630 1640 1650
SLQSSGGRQV APKSKDQVSN STTEQDHLLV QCKYVLELLL AIPKVTLARA
1660 1670 1680 1690 1700
SFRCQAYARS LMYLESHVRG KSGSLNPAAE KTGIFENADV SSLMGIYSCL
1710 1720 1730 1740 1750
DEPDGLSGFA SLSKSLNLQD QLLINKKSGN WADVFTACEQ ALQMEPTSVQ
1760 1770 1780 1790 1800
RHSDVLNCLL NMCHHQTMVT HVDGLISRVP EYKKTWCTQG VQAAWRLGKW
1810 1820 1830 1840 1850
DLMDEYLDGA DAEGLLFSSS DSNASFDRDV AKILHAMMKK DQYSVAEGIA
1860 1870 1880 1890 1900
ISKQALIAPL AAAGMDSYTR AYPFVVKLHL LRELEDFQAV LNGDSYLEKS
1910 1920 1930 1940 1950
FSTSDQVFSK AVDNWENRLR FTQSSLWTRE PLLAFRRLVF GASGLGAQVG
1960 1970 1980 1990 2000
NCWLQYAKLC RLAGHYETAH RAILEAQASG APNVHMEKAK LLWITKRSDS
2010 2020 2030 2040 2050
AIIELQQSLL NMPEGVVDST VISSINSLLM APPNPEPTVR NTQSFKEKKD
2060 2070 2080 2090 2100
VAKTLLLYSK WIHHSGQKQK KDVLNLYTQV KELLPWEKGY FHLAKYYDEL
2110 2120 2130 2140 2150
YVDARKCQQE SSVFSSAGSK KGSVSSNLST EKAGWDYLFK GMYFYAKALH
2160 2170 2180 2190 2200
SGHKNLFQAL PRLLTLWFDF GTIYKTSGSA GNKELKSTHM KIMSLMRGCL
2210 2220 2230 2240 2250
KDLPTYQWLT VLPQLVSRIC HQNADTVLMV KNIITSVLHQ FPQQGLWIMA
2260 2270 2280 2290 2300
AVSKSTVPAR REAAAEIIQG ARKGFNQSDR GHNLFIQFAS LTDHFIKLCF
2310 2320 2330 2340 2350
HGGQPRSKVI NIATEFSALK RMMPLDIIMP IQQSLTISLP AFHMNNNERH
2360 2370 2380 2390 2400
SASVFSGSDL PTISGIADEA EILSSLQRPK KIILLGNDGI EYPFLCKPKD
2410 2420 2430 2440 2450
DLRKDARMME FTAMINRLLS KYPESRRRKL YIRTFAVAPL TEDCGLVEWV
2460 2470 2480 2490 2500
PHTRGLRHIL QDIYISCGKF DRQKTNPQIK RIYDQCAVKK EYEMLKTKIL
2510 2520 2530 2540 2550
PMFPPVFHKW FLTTFSEPAA WFRSRVAYAH TTAVWSMVGH IVGLGDRHGE
2560 2570 2580 2590 2600
NILFDSTSGD CVHVDFSCLF DKGLQLEKPE LVPFRLTQNM IDGLGITGYE
2610 2620 2630 2640 2650
GIFMRVCEIT LTVLRTHRET LMSILETFIH DPLVEWTKSH KSSGVEVQNP
2660 2670 2680 2690 2700
HAQRAISSIE ARLQGVVVGV PLPVEGQARR LIADAVSLEN LGKMYIWWMP

WF
Length:2,702
Mass (Da):302,366
Last modified:November 23, 2004 - v2
Checksum:iDCC88C59F50E23D3
GO

Sequence cautioni

The sequence BAB11344.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti152 – 1532AS → GIH in BAA92828 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB040133 mRNA. Translation: BAA92828.1.
AB011477 Genomic DNA. Translation: BAB11344.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED94599.1.
RefSeqiNP_198898.2. NM_123447.2.
UniGeneiAt.475.

Genome annotation databases

EnsemblPlantsiAT5G40820.1; AT5G40820.1; AT5G40820.
GeneIDi834082.
GrameneiAT5G40820.1; AT5G40820.1; AT5G40820.
KEGGiath:AT5G40820.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB040133 mRNA. Translation: BAA92828.1.
AB011477 Genomic DNA. Translation: BAB11344.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED94599.1.
RefSeqiNP_198898.2. NM_123447.2.
UniGeneiAt.475.

3D structure databases

ProteinModelPortaliQ9FKS4.
SMRiQ9FKS4. Positions 1653-2298.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G40820.1.

PTM databases

iPTMnetiQ9FKS4.

Proteomic databases

PaxDbiQ9FKS4.
PRIDEiQ9FKS4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G40820.1; AT5G40820.1; AT5G40820.
GeneIDi834082.
GrameneiAT5G40820.1; AT5G40820.1; AT5G40820.
KEGGiath:AT5G40820.

Organism-specific databases

TAIRiAT5G40820.

Phylogenomic databases

eggNOGiKOG0890. Eukaryota.
COG5032. LUCA.
HOGENOMiHOG000030210.
InParanoidiQ9FKS4.
KOiK06640.
OMAiMRGCLKD.
PhylomeDBiQ9FKS4.

Enzyme and pathway databases

BioCyciARA:AT5G40820-MONOMER.
ReactomeiR-ATH-3371453. Regulation of HSF1-mediated heat shock response.

Miscellaneous databases

PROiQ9FKS4.

Gene expression databases

GenevisibleiQ9FKS4. AT.

Family and domain databases

Gene3Di1.10.1070.11. 2 hits.
1.25.40.10. 2 hits.
InterProiIPR016024. ARM-type_fold.
IPR003152. FATC_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR003151. PIK-rel_kinase_FAT.
IPR014009. PIK_FAT.
IPR011990. TPR-like_helical_dom.
IPR012993. UME.
[Graphical view]
PfamiPF02259. FAT. 1 hit.
PF02260. FATC. 1 hit.
PF00454. PI3_PI4_kinase. 1 hit.
PF08064. UME. 1 hit.
[Graphical view]
SMARTiSM01343. FATC. 1 hit.
SM00146. PI3Kc. 1 hit.
SM00802. UME. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 5 hits.
SSF56112. SSF56112. 2 hits.
PROSITEiPS51189. FAT. 1 hit.
PS51190. FATC. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis thaliana mRNA for AtRAD3, complete cds."
    Sugiyama H., Oguchi K., Tamura K., Takahashi H.
    Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones."
    Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "ATR regulates a G2-phase cell-cycle checkpoint in Arabidopsis thaliana."
    Culligan K., Tissier A., Britt A.
    Plant Cell 16:1091-1104(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  5. "ATM and ATR make distinct contributions to chromosome end protection and the maintenance of telomeric DNA in Arabidopsis."
    Vespa L., Couvillion M., Spangler E., Shippen D.E.
    Genes Dev. 19:2111-2115(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Ionizing radiation-dependent gamma-H2AX focus formation requires ataxia telangiectasia mutated and ataxia telangiectasia mutated and Rad3-related."
    Friesner J.D., Liu B., Culligan K., Britt A.B.
    Mol. Biol. Cell 16:2566-2576(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiATR_ARATH
AccessioniPrimary (citable) accession number: Q9FKS4
Secondary accession number(s): Q9MAZ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: November 23, 2004
Last modified: May 11, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Loss-of-function mutations (T-DNA insertion) are hypersensitive to replication antagonists, including UV light, aphidicolin, and hydroxyurea, and are defective in G2 checkpoints induced by these agents.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.