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Protein

Uridine kinase-like protein 1, chloroplastic

Gene

UKL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the pyrimidine salvage pathway. The uracil phosphoribosyltransferase (UPRT) activity, that catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate, is unsure.2 Publications

Catalytic activityi

UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate.
ATP + cytidine = ADP + CMP.
ATP + uridine = ADP + UMP.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit. The magnesium is bound as Mg-PRPP.By similarity

Enzyme regulationi

Allosterically activated by GTP.By similarity

Pathwayi: UMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from uracil.
Proteins known to be involved in this subpathway in this organism are:
  1. Uridine kinase-like protein 5 (UKL5), Uridine kinase-like protein 4 (UKL4), Uridine kinase-like protein 1, chloroplastic (UKL1), Uridine kinase-like protein 2, chloroplastic (UKL2), Uridine kinase-like protein 3 (UKL3), Uracil phosphoribosyltransferase, chloroplastic (UPP)
This subpathway is part of the pathway UMP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from uracil, the pathway UMP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Pathwayi: CTP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes CTP from cytidine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Uridine kinase-like protein 5 (UKL5), Uridine kinase-like protein 4 (UKL4), Uridine kinase (AXX17_At1g50440), Uridine kinase-like protein 1, chloroplastic (UKL1), Uridine kinase-like protein 2, chloroplastic (UKL2), Uridine kinase (AXX17_At4g30590), Uridine kinase (AXX17_At5g38600), Uridine kinase (AXX17_At1g50440), Uridine kinase (AXX17_At3g29950), Uridine kinase (AXX17_At3g29630), Uridine kinase-like protein 3 (UKL3)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
This subpathway is part of the pathway CTP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CTP from cytidine, the pathway CTP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Pathwayi: UMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from uridine.
Proteins known to be involved in this subpathway in this organism are:
  1. Uridine kinase-like protein 5 (UKL5), Uridine kinase-like protein 4 (UKL4), Uridine kinase (AXX17_At1g50440), Uridine kinase-like protein 1, chloroplastic (UKL1), Uridine kinase-like protein 2, chloroplastic (UKL2), Uridine kinase (AXX17_At4g30590), Uridine kinase (AXX17_At5g38600), Uridine kinase (AXX17_At1g50440), Uridine kinase (AXX17_At3g29950), Uridine kinase (AXX17_At3g29630), Uridine kinase-like protein 3 (UKL3)
This subpathway is part of the pathway UMP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from uridine, the pathway UMP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei298GTPBy similarity1
Binding sitei307GTPBy similarity1
Binding sitei3515-phospho-alpha-D-ribose 1-diphosphateBy similarity1
Binding sitei3765-phospho-alpha-D-ribose 1-diphosphateBy similarity1
Binding sitei396GTPBy similarity1
Binding sitei4025-phospho-alpha-D-ribose 1-diphosphateBy similarity1
Binding sitei4735-phospho-alpha-D-ribose 1-diphosphateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi341 – 344GTPBy similarity4

GO - Molecular functioni

  • ATP binding Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • uracil phosphoribosyltransferase activity Source: TAIR
  • uridine kinase activity Source: TAIR

GO - Biological processi

  • CTP salvage Source: UniProtKB-UniPathway
  • pyrimidine nucleobase metabolic process Source: GO_Central
  • regulation of starch metabolic process Source: TAIR
  • UMP salvage Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Kinase, Transferase

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G40870-MONOMER.
BRENDAi2.4.2.9. 399.
2.7.1.48. 399.
ReactomeiR-ATH-73614. Pyrimidine salvage reactions.
UniPathwayiUPA00574; UER00636.
UPA00574; UER00637.
UPA00579; UER00640.

Names & Taxonomyi

Protein namesi
Recommended name:
Uridine kinase-like protein 1, chloroplastic
Including the following 2 domains:
Uridine kinase (EC:2.7.1.48)
Short name:
UK
Putative uracil phosphoribosyltransferase (EC:2.4.2.9)
Short name:
UPRTase
Alternative name(s):
UMP pyrophosphorylase
Gene namesi
Name:UKL1
Synonyms:UK/UPRT1
Ordered Locus Names:At5g40870
ORF Names:MHK7.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G40870.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • cytoplasm Source: TAIR
  • cytosol Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Cytoplasm, Plastid

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. No decrease in uracil phosphoribosyltransferase activity. Loss of sensitivity to 5'-fluorouracil and 5'-fluorouridine.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 47ChloroplastSequence analysisAdd BLAST47
ChainiPRO_000039451448 – 486Uridine kinase-like protein 1, chloroplasticAdd BLAST439

Proteomic databases

PaxDbiQ9FKS0.

PTM databases

iPTMnetiQ9FKS0.

Expressioni

Tissue specificityi

Expressed in leaves, roots, stems and flowers.1 Publication

Gene expression databases

GenevisibleiQ9FKS0. AT.

Interactioni

Protein-protein interaction databases

BioGridi19339. 1 interactor.
STRINGi3702.AT5G40870.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FKS0.
SMRiQ9FKS0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni59 – 264Uridine kinaseAdd BLAST206
Regioni274 – 486Uracil phosphoribosyltransferaseAdd BLAST213
Regioni407 – 4105-phospho-alpha-D-ribose 1-diphosphate bindingBy similarity4
Regioni472 – 474Uracil bindingBy similarity3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi47 – 54Poly-Ser8

Sequence similaritiesi

In the N-terminal section; belongs to the uridine kinase family.Curated
In the C-terminal section; belongs to the UPRTase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG4203. Eukaryota.
COG0035. LUCA.
COG0572. LUCA.
HOGENOMiHOG000262757.
InParanoidiQ9FKS0.
KOiK00876.
OMAiNGHIDDR.
OrthoDBiEOG093609CS.
PhylomeDBiQ9FKS0.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000836. PRibTrfase_dom.
IPR006083. PRK/URK.
IPR029057. PRTase-like.
IPR029930. UCKL_plant.
IPR000764. Uridine_kinase-like.
[Graphical view]
PANTHERiPTHR10285:SF71. PTHR10285:SF71. 1 hit.
PfamiPF00485. PRK. 1 hit.
PF14681. UPRTase. 1 hit.
[Graphical view]
PRINTSiPR00988. URIDINKINASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00235. udk. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FKS0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEDSSSLDY AMEKASGPHF SGLRFDGLLS SSPPNSSVVS SLRSAVSSSS
60 70 80 90 100
PSSSDPEAPK QPFIIGVSGG TASGKTTVCD MIIQQLHDHR VVLVNQDSFY
110 120 130 140 150
RGLTSEELQR VQEYNFDHPD AFDTEQLLHC AETLKSGQPY QVPIYDFKTH
160 170 180 190 200
QRRSDTFRQV NASDVIILEG ILVFHDSRVR NLMNMKIFVD TDADVRLARR
210 220 230 240 250
IRRDTVERGR DVNSVLEQYA KFVKPAFDDF VLPSKKYADV IIPRGGDNHV
260 270 280 290 300
AVDLITQHIH TKLGQHDLCK IYPNVYVIQS TFQIRGMHTL IREKDISKHD
310 320 330 340 350
FVFYSDRLIR LVVEHGLGHL PFTEKQVVTP TGAVYTGVDF CKKLCGVSII
360 370 380 390 400
RSGESMENAL RACCKGIKIG KILIHRDGDN GKQLIYEKLP HDISERHVLL
410 420 430 440 450
LDPVLATGNS ANQAIELLIQ KGVPEAHIIF LNLISAPEGI HCVCKRFPAL
460 470 480
KIVTSEIDQC LNQEFRVIPG LGEFGDRYFG TDEEDQ
Length:486
Mass (Da):54,430
Last modified:March 1, 2001 - v1
Checksum:i50DA1CE89346FB54
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY089970 mRNA. Translation: AAM10488.1.
AB011477 Genomic DNA. Translation: BAB11349.1.
CP002688 Genomic DNA. Translation: AED94610.1.
AY080631 mRNA. Translation: AAL85977.1.
BT002336 mRNA. Translation: AAN86169.1.
RefSeqiNP_198903.1. NM_123452.5.
UniGeneiAt.9230.

Genome annotation databases

EnsemblPlantsiAT5G40870.1; AT5G40870.1; AT5G40870.
GeneIDi834088.
GrameneiAT5G40870.1; AT5G40870.1; AT5G40870.
KEGGiath:AT5G40870.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY089970 mRNA. Translation: AAM10488.1.
AB011477 Genomic DNA. Translation: BAB11349.1.
CP002688 Genomic DNA. Translation: AED94610.1.
AY080631 mRNA. Translation: AAL85977.1.
BT002336 mRNA. Translation: AAN86169.1.
RefSeqiNP_198903.1. NM_123452.5.
UniGeneiAt.9230.

3D structure databases

ProteinModelPortaliQ9FKS0.
SMRiQ9FKS0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19339. 1 interactor.
STRINGi3702.AT5G40870.1.

PTM databases

iPTMnetiQ9FKS0.

Proteomic databases

PaxDbiQ9FKS0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G40870.1; AT5G40870.1; AT5G40870.
GeneIDi834088.
GrameneiAT5G40870.1; AT5G40870.1; AT5G40870.
KEGGiath:AT5G40870.

Organism-specific databases

TAIRiAT5G40870.

Phylogenomic databases

eggNOGiKOG4203. Eukaryota.
COG0035. LUCA.
COG0572. LUCA.
HOGENOMiHOG000262757.
InParanoidiQ9FKS0.
KOiK00876.
OMAiNGHIDDR.
OrthoDBiEOG093609CS.
PhylomeDBiQ9FKS0.

Enzyme and pathway databases

UniPathwayiUPA00574; UER00636.
UPA00574; UER00637.
UPA00579; UER00640.
BioCyciARA:AT5G40870-MONOMER.
BRENDAi2.4.2.9. 399.
2.7.1.48. 399.
ReactomeiR-ATH-73614. Pyrimidine salvage reactions.

Miscellaneous databases

PROiQ9FKS0.

Gene expression databases

GenevisibleiQ9FKS0. AT.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000836. PRibTrfase_dom.
IPR006083. PRK/URK.
IPR029057. PRTase-like.
IPR029930. UCKL_plant.
IPR000764. Uridine_kinase-like.
[Graphical view]
PANTHERiPTHR10285:SF71. PTHR10285:SF71. 1 hit.
PfamiPF00485. PRK. 1 hit.
PF14681. UPRTase. 1 hit.
[Graphical view]
PRINTSiPR00988. URIDINKINASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00235. udk. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUKL1_ARATH
AccessioniPrimary (citable) accession number: Q9FKS0
Secondary accession number(s): Q8RXX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Experiments shown in PubMed:17143579 indicate an uracil phosphoribosyltransferase (UPRT) activity, while PubMed:19563437 shows a lack of such activity.Curated

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.