Uridine kinase-like protein 1, chloroplastic precursor - Arabidopsis thaliana (Mouse-ear cress)

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Q9FKS0 (UKL1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 86. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Uridine kinase-like protein 1, chloroplastic

Including the following 2 domains:

Uridine kinase
Short name=UK
EC=2.7.1.48
Putative uracil phosphoribosyltransferase
Short name=UPRTase
EC=2.4.2.9
Alternative name(s):
UMP pyrophosphorylase

Gene names

Name:	UKL1
Synonyms:	UK/UPRT1
Ordered Locus Names:	At5g40870
ORF Names:	MHK7.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the pyrimidine salvage pathway. The uracil phosphoribosyltransferase (UPRT) activity, that catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate, is unsure. Ref.1 Ref.6
Catalytic activity	UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate. ATP + cytidine = ADP + CMP. ATP + uridine = ADP + UMP.
Cofactor	Binds 1 Mg²⁺ ion per subunit. The magnesium is bound as Mg-PRPP By similarity.
Enzyme regulation	Allosterically activated by GTP By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1. Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3. Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.
Subcellular location	Plastid › chloroplast Potential. Cytoplasm. Note: Aggregates in granular precipitates restricted to the cytoplasm, when expressed in transfected cells. Ref.1
Tissue specificity	Expressed in leaves, roots, stems and flowers. Ref.1
Disruption phenotype	No visible phenotype. No decrease in uracil phosphoribosyltransferase activity. Loss of sensitivity to 5'-fluorouracil and 5'-fluorouridine. Ref.1 Ref.6
Sequence similarities	In the N-terminal section; belongs to the uridine kinase family. In the C-terminal section; belongs to the UPRTase family.
Caution	Experiments shown in Ref.1 indicate an uracil phosphoribosyltransferase (UPRT) activity, while Ref.6 shows a lack of such activity.

Ontologies

Keywords
Cellular component	Chloroplast Cytoplasm Plastid
Domain	Transit peptide
Ligand	GTP-binding Nucleotide-binding
Molecular function	Glycosyltransferase Kinase Transferase
PTM	Phosphoprotein
Technical term	Allosteric enzyme Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	CTP salvage Inferred from electronic annotation. Source: UniProtKB-UniPathway UMP salvage Inferred from direct assay PubMed 21828290. Source: TAIR regulation of starch metabolic process Inferred from mutant phenotype PubMed 21828290. Source: TAIR
Cellular_component	chloroplast Inferred from direct assay PubMed 21828290. Source: TAIR cytosol Inferred from direct assay PubMed 21166475. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: InterPro GTP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphotransferase activity, alcohol group as acceptor Inferred from electronic annotation. Source: InterPro uracil phosphoribosyltransferase activity Inferred from genetic interaction Ref.1. Source: TAIR uridine kinase activity Inferred from direct assay PubMed 21828290. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 47

Chloroplast Potential

Chain

48 – 486

439

Uridine kinase-like protein 1, chloroplastic

PRO_0000394514

Regions

Nucleotide binding

341 – 344

GTP By similarity

Region

59 – 264

206

Uridine kinase

Region

274 – 486

213

Uracil phosphoribosyltransferase

Region

407 – 410

5-phospho-alpha-D-ribose 1-diphosphate binding By similarity

Region

472 – 474

Uracil binding By similarity

Compositional bias

47 – 54

Poly-Ser

Sites

Binding site

298

GTP By similarity

Binding site

307

GTP By similarity

Binding site

351

5-phospho-alpha-D-ribose 1-diphosphate By similarity

Binding site

376

5-phospho-alpha-D-ribose 1-diphosphate By similarity

Binding site

396

GTP By similarity

Binding site

402

5-phospho-alpha-D-ribose 1-diphosphate By similarity

Binding site

473

5-phospho-alpha-D-ribose 1-diphosphate By similarity

Amino acid modifications

Modified residue

Phosphoserine Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

Q9FKS0 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 50DA1CE89346FB54
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FASTA

486

54,430

        10         20         30         40         50         60 
MPEDSSSLDY AMEKASGPHF SGLRFDGLLS SSPPNSSVVS SLRSAVSSSS PSSSDPEAPK 

        70         80         90        100        110        120 
QPFIIGVSGG TASGKTTVCD MIIQQLHDHR VVLVNQDSFY RGLTSEELQR VQEYNFDHPD 

       130        140        150        160        170        180 
AFDTEQLLHC AETLKSGQPY QVPIYDFKTH QRRSDTFRQV NASDVIILEG ILVFHDSRVR 

       190        200        210        220        230        240 
NLMNMKIFVD TDADVRLARR IRRDTVERGR DVNSVLEQYA KFVKPAFDDF VLPSKKYADV 

       250        260        270        280        290        300 
IIPRGGDNHV AVDLITQHIH TKLGQHDLCK IYPNVYVIQS TFQIRGMHTL IREKDISKHD 

       310        320        330        340        350        360 
FVFYSDRLIR LVVEHGLGHL PFTEKQVVTP TGAVYTGVDF CKKLCGVSII RSGESMENAL 

       370        380        390        400        410        420 
RACCKGIKIG KILIHRDGDN GKQLIYEKLP HDISERHVLL LDPVLATGNS ANQAIELLIQ 

       430        440        450        460        470        480 
KGVPEAHIIF LNLISAPEGI HCVCKRFPAL KIVTSEIDQC LNQEFRVIPG LGEFGDRYFG 


TDEEDQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Functional characterization of a gene encoding a dual domain for uridine kinase and uracil phosphoribosyltransferase in Arabidopsis thaliana." Islam M.R., Kim H., Kang S.W., Kim J.S., Jeong Y.M., Hwang H.J., Lee S.Y., Woo J.C., Kim S.G. Plant Mol. Biol. 63:465-477(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones." Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S. DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks." Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S. Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, MASS SPECTROMETRY. Strain: cv. Columbia. Tissue: Seedling.
[6]	"Uracil salvage is necessary for early Arabidopsis development." Mainguet S.E., Gakiere B., Majira A., Pelletier S., Bringel F., Guerard F., Caboche M., Berthome R., Renou J.P. Plant J. 60:280-291(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY089970 mRNA. Translation: AAM10488.1. AB011477 Genomic DNA. Translation: BAB11349.1. CP002688 Genomic DNA. Translation: AED94610.1. AY080631 mRNA. Translation: AAL85977.1. BT002336 mRNA. Translation: AAN86169.1.
IPI	IPI00518670.
RefSeq	NP_198903.1. NM_123452.4.
UniGene	At.9230.
3D structure databases
HSSP	HSSP built from PDB template 1JLS based on UniProtKB Q26998.
ProteinModelPortal	Q9FKS0.
SMR	Q9FKS0. Positions 61-263, 266-481.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT5G40870.1-P.
Proteomic databases
PaxDb	Q9FKS0.
PRIDE	Q9FKS0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G40870.1; AT5G40870.1; AT5G40870.
GeneID	834088.
KEGG	ath:AT5G40870.
Organism-specific databases
TAIR	At5g40870.
Phylogenomic databases
eggNOG	COG0572.
HOGENOM	HOG000262757.
InParanoid	Q9FKS0.
KO	K00876.
OMA	CEDRSNA.
PhylomeDB	Q9FKS0.
ProtClustDB	CLSN2682673.
Enzyme and pathway databases
BRENDA	2.4.2.9. 399.
UniPathway	UPA00574; UER00636. UPA00574; UER00637. UPA00579; UER00640.
Gene expression databases
Genevestigator	Q9FKS0.
Family and domain databases
InterPro	IPR006083. PRK/URK. IPR000764. Uridine_kinase. IPR026008. Uridine_kinase-like. [Graphical view]
PANTHER	PTHR10285:SF6. PTHR10285:SF6. 1 hit.
Pfam	PF00485. PRK. 1 hit. [Graphical view]
PRINTS	PR00988. URIDINKINASE.
TIGRFAMs	TIGR00235. udk. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UKL1_ARATH

Accession

Primary (citable) accession number: Q9FKS0
Secondary accession number(s): Q8RXX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 15, 2010
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Including the following 2 domains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents