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Q9FKP5 (UBP17_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 17
EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 17
Short name=AtUBP17
Ubiquitin thioesterase 17
Ubiquitin-specific-processing protease 17
Gene names
Name:UBP17
Ordered Locus Names:At5g65450
ORF Names:MNA5.19
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length731 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 MYND-type zinc finger.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9FKP5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9FKP5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     421-448: CAVDTMQSVFLKEAPAAGPFAEETTLVG → MPGVLLIQCNLFFSKRLLQLVRLLKKLL
     449-731: Missing.
Note: May be due to a competing acceptor splice site. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 731731Ubiquitin carboxyl-terminal hydrolase 17
PRO_0000313043

Regions

Zinc finger57 – 9438MYND-type
Compositional bias245 – 31268Ser-rich
Compositional bias679 – 70224Ser-rich

Sites

Active site3381Nucleophile By similarity
Active site5921Proton acceptor By similarity

Natural variations

Alternative sequence421 – 44828CAVDT…TTLVG → MPGVLLIQCNLFFSKRLLQL VRLLKKLL in isoform 2.
VSP_029990
Alternative sequence449 – 731283Missing in isoform 2.
VSP_029991

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: D17844AC368057B5

FASTA73181,999
        10         20         30         40         50         60 
MMLVFLLIRR QWRSASVRRE EVIRLIALAT EESYLAEEVR PATVDYGGDS VSDVYRCAVC 

        70         80         90        100        110        120 
LYPTTTRCSQ CKSVRYCSSK CQILHWRRGH KEECRSPDYD EEKEEYVQSD YDAKESNVDF 

       130        140        150        160        170        180 
PSRGTAYESS SNVSVDVACD MSTSRPSIHK VQPRSEAVDF TTSLNIKDNL YETRPLSRKK 

       190        200        210        220        230        240 
SRNRTDKVES ASNYSKGKTD AKLRKLGNQN SRRSGDSANM SISDQFLSVG FEEEMNALKH 

       250        260        270        280        290        300 
ERITSEPSSA SAAMSSSSTL LLPSKANSKP KVSQASSSGL KTSVQKVVQH FRPPQSSKKS 

       310        320        330        340        350        360 
QPSSSIDEMS FSYELFVKLY CDRVELQPFG LVNLGNSCYA NAVLQCLAFT RPLISYLIRG 

       370        380        390        400        410        420 
LHSKTCRKKS WCFVCEFEHL ILKARGGESP LSPIKILSKL QKIGKHLGPG KEEDAHEFLR 

       430        440        450        460        470        480 
CAVDTMQSVF LKEAPAAGPF AEETTLVGLT FGGYLHSKIK CMACLHKSER PELMMDLTVE 

       490        500        510        520        530        540 
IDGDIGSLEE ALAQFTAYEV LDGENRYFCG RCKSYQKAKK KLMILEGPNI LTVVLKRFQS 

       550        560        570        580        590        600 
DNFGKLSKPI HFPELLDISP YMSDPNHGDH PVYSLYAVVV HLDAMSTLFS GHYVCYIKTL 

       610        620        630        640        650        660 
DGDWFKIDDS NVFPVQLETV LLEGAYMLLY ARDSPRPVSK NGGRKSKQRR NLAAIPSRKG 

       670        680        690        700        710        720 
NKKQRDGDNN SLLPRVDWSS GSLSSMFSSS DTTSSCSTKD SSGIENLSDY LFGGVEPVWK 

       730 
WDRHNKSQTF D

« Hide

Isoform 2 [UniParc].

Checksum: C10389FE47E17BA5
Show »

FASTA44850,543

References

« Hide 'large scale' references
[1]"The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), GENE FAMILY ORGANIZATION, NOMENCLATURE.
Strain: cv. Columbia.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones."
Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S.
DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF302667 mRNA. No translation available.
AB011479 Genomic DNA. Translation: BAB11567.1.
CP002688 Genomic DNA. Translation: AED98058.1.
IPIIPI00540431.
IPI00882576.
RefSeqNP_201348.1. NM_125943.1.
UniGeneAt.16943.

3D structure databases

HSSPHSSP built from PDB template 2AYN based on UniProtKB P54578.
ProteinModelPortalQ9FKP5.
SMRQ9FKP5. Positions 57-96, 330-630.
ModBaseSearch...

Protein family/group databases

MEROPSC19.A06.

Proteomic databases

PaxDbQ9FKP5.
PRIDEQ9FKP5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G65450.1; AT5G65450.1; AT5G65450.
GeneID836670.
KEGGath:AT5G65450.

Organism-specific databases

TAIRAt5g65450.

Phylogenomic databases

eggNOGCOG5533.
HOGENOMHOG000242869.
InParanoidQ9FKP5.
KOK11855.
OMALEGAYML.
PhylomeDBQ9FKP5.
ProtClustDBCLSN2686475.

Gene expression databases

GenevestigatorQ9FKP5.

Family and domain databases

InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19.
IPR002893. Znf_MYND.
[Graphical view]
PfamPF00443. UCH. 1 hit.
PF01753. zf-MYND. 1 hit.
[Graphical view]
PROSITEPS00972. UCH_2_1. 1 hit.
PS00973. UCH_2_2. False negative.
PS50235. UCH_2_3. 1 hit.
PS01360. ZF_MYND_1. 1 hit.
PS50865. ZF_MYND_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBP17_ARATH
AccessionPrimary (citable) accession number: Q9FKP5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2001
Last modified: May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents