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Q9FKP5

- UBP17_ARATH

UniProt

Q9FKP5 - UBP17_ARATH

Protein

Ubiquitin carboxyl-terminal hydrolase 17

Gene

UBP17

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei338 – 3381NucleophilePROSITE-ProRule annotation
    Active sitei592 – 5921Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri57 – 9438MYND-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. ubiquitinyl hydrolase activity Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciARA:AT5G65450-MONOMER.

    Protein family/group databases

    MEROPSiC19.A06.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 17 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 17
    Short name:
    AtUBP17
    Ubiquitin thioesterase 17
    Ubiquitin-specific-processing protease 17
    Gene namesi
    Name:UBP17
    Ordered Locus Names:At5g65450
    ORF Names:MNA5.19
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G65450.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 731731Ubiquitin carboxyl-terminal hydrolase 17PRO_0000313043Add
    BLAST

    Proteomic databases

    PaxDbiQ9FKP5.
    PRIDEiQ9FKP5.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9FKP5.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT5G65450.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FKP5.
    SMRiQ9FKP5. Positions 330-630.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini329 – 633305USPAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi245 – 31268Ser-richAdd
    BLAST
    Compositional biasi679 – 70224Ser-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 MYND-type zinc finger.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri57 – 9438MYND-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5533.
    HOGENOMiHOG000242869.
    InParanoidiQ9FKP5.
    KOiK11855.
    OMAiQSICLKE.
    PhylomeDBiQ9FKP5.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR002893. Znf_MYND.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    PF01753. zf-MYND. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS50235. USP_3. 1 hit.
    PS01360. ZF_MYND_1. 1 hit.
    PS50865. ZF_MYND_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9FKP5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MMLVFLLIRR QWRSASVRRE EVIRLIALAT EESYLAEEVR PATVDYGGDS    50
    VSDVYRCAVC LYPTTTRCSQ CKSVRYCSSK CQILHWRRGH KEECRSPDYD 100
    EEKEEYVQSD YDAKESNVDF PSRGTAYESS SNVSVDVACD MSTSRPSIHK 150
    VQPRSEAVDF TTSLNIKDNL YETRPLSRKK SRNRTDKVES ASNYSKGKTD 200
    AKLRKLGNQN SRRSGDSANM SISDQFLSVG FEEEMNALKH ERITSEPSSA 250
    SAAMSSSSTL LLPSKANSKP KVSQASSSGL KTSVQKVVQH FRPPQSSKKS 300
    QPSSSIDEMS FSYELFVKLY CDRVELQPFG LVNLGNSCYA NAVLQCLAFT 350
    RPLISYLIRG LHSKTCRKKS WCFVCEFEHL ILKARGGESP LSPIKILSKL 400
    QKIGKHLGPG KEEDAHEFLR CAVDTMQSVF LKEAPAAGPF AEETTLVGLT 450
    FGGYLHSKIK CMACLHKSER PELMMDLTVE IDGDIGSLEE ALAQFTAYEV 500
    LDGENRYFCG RCKSYQKAKK KLMILEGPNI LTVVLKRFQS DNFGKLSKPI 550
    HFPELLDISP YMSDPNHGDH PVYSLYAVVV HLDAMSTLFS GHYVCYIKTL 600
    DGDWFKIDDS NVFPVQLETV LLEGAYMLLY ARDSPRPVSK NGGRKSKQRR 650
    NLAAIPSRKG NKKQRDGDNN SLLPRVDWSS GSLSSMFSSS DTTSSCSTKD 700
    SSGIENLSDY LFGGVEPVWK WDRHNKSQTF D 731
    Length:731
    Mass (Da):81,999
    Last modified:March 1, 2001 - v1
    Checksum:iD17844AC368057B5
    GO
    Isoform 2 (identifier: Q9FKP5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         421-448: CAVDTMQSVFLKEAPAAGPFAEETTLVG → MPGVLLIQCNLFFSKRLLQLVRLLKKLL
         449-731: Missing.

    Note: May be due to a competing acceptor splice site. No experimental confirmation available.

    Show »
    Length:448
    Mass (Da):50,543
    Checksum:iC10389FE47E17BA5
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei421 – 44828CAVDT…TTLVG → MPGVLLIQCNLFFSKRLLQL VRLLKKLL in isoform 2. 1 PublicationVSP_029990Add
    BLAST
    Alternative sequencei449 – 731283Missing in isoform 2. 1 PublicationVSP_029991Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF302667 mRNA. No translation available.
    AB011479 Genomic DNA. Translation: BAB11567.1.
    CP002688 Genomic DNA. Translation: AED98058.1.
    RefSeqiNP_201348.1. NM_125943.1. [Q9FKP5-1]
    UniGeneiAt.16943.

    Genome annotation databases

    EnsemblPlantsiAT5G65450.1; AT5G65450.1; AT5G65450. [Q9FKP5-1]
    GeneIDi836670.
    KEGGiath:AT5G65450.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF302667 mRNA. No translation available.
    AB011479 Genomic DNA. Translation: BAB11567.1 .
    CP002688 Genomic DNA. Translation: AED98058.1 .
    RefSeqi NP_201348.1. NM_125943.1. [Q9FKP5-1 ]
    UniGenei At.16943.

    3D structure databases

    ProteinModelPortali Q9FKP5.
    SMRi Q9FKP5. Positions 330-630.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT5G65450.1-P.

    Protein family/group databases

    MEROPSi C19.A06.

    Proteomic databases

    PaxDbi Q9FKP5.
    PRIDEi Q9FKP5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G65450.1 ; AT5G65450.1 ; AT5G65450 . [Q9FKP5-1 ]
    GeneIDi 836670.
    KEGGi ath:AT5G65450.

    Organism-specific databases

    TAIRi AT5G65450.

    Phylogenomic databases

    eggNOGi COG5533.
    HOGENOMi HOG000242869.
    InParanoidi Q9FKP5.
    KOi K11855.
    OMAi QSICLKE.
    PhylomeDBi Q9FKP5.

    Enzyme and pathway databases

    BioCyci ARA:AT5G65450-MONOMER.

    Gene expression databases

    Genevestigatori Q9FKP5.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR002893. Znf_MYND.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    PF01753. zf-MYND. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS50235. USP_3. 1 hit.
    PS01360. ZF_MYND_1. 1 hit.
    PS50865. ZF_MYND_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
      Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
      Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), GENE FAMILY ORGANIZATION, NOMENCLATURE.
      Strain: cv. Columbia.
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones."
      Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S.
      DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiUBP17_ARATH
    AccessioniPrimary (citable) accession number: Q9FKP5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3