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Protein

Ubiquitin carboxyl-terminal hydrolase 17

Gene

UBP17

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei338 – 3381NucleophilePROSITE-ProRule annotation
Active sitei592 – 5921Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri57 – 9438MYND-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT5G65450-MONOMER.

Protein family/group databases

MEROPSiC19.A06.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 17 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 17
Short name:
AtUBP17
Ubiquitin thioesterase 17
Ubiquitin-specific-processing protease 17
Gene namesi
Name:UBP17
Ordered Locus Names:At5g65450
ORF Names:MNA5.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G65450.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 731731Ubiquitin carboxyl-terminal hydrolase 17PRO_0000313043Add
BLAST

Proteomic databases

PaxDbiQ9FKP5.
PRIDEiQ9FKP5.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G65450.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FKP5.
SMRiQ9FKP5. Positions 330-630.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini329 – 633305USPAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi245 – 31268Ser-richAdd
BLAST
Compositional biasi679 – 70224Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 MYND-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri57 – 9438MYND-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5533.
HOGENOMiHOG000242869.
InParanoidiQ9FKP5.
KOiK11855.
OMAiHEFLRCA.
PhylomeDBiQ9FKP5.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR002893. Znf_MYND.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF01753. zf-MYND. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS50235. USP_3. 1 hit.
PS01360. ZF_MYND_1. 1 hit.
PS50865. ZF_MYND_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9FKP5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMLVFLLIRR QWRSASVRRE EVIRLIALAT EESYLAEEVR PATVDYGGDS
60 70 80 90 100
VSDVYRCAVC LYPTTTRCSQ CKSVRYCSSK CQILHWRRGH KEECRSPDYD
110 120 130 140 150
EEKEEYVQSD YDAKESNVDF PSRGTAYESS SNVSVDVACD MSTSRPSIHK
160 170 180 190 200
VQPRSEAVDF TTSLNIKDNL YETRPLSRKK SRNRTDKVES ASNYSKGKTD
210 220 230 240 250
AKLRKLGNQN SRRSGDSANM SISDQFLSVG FEEEMNALKH ERITSEPSSA
260 270 280 290 300
SAAMSSSSTL LLPSKANSKP KVSQASSSGL KTSVQKVVQH FRPPQSSKKS
310 320 330 340 350
QPSSSIDEMS FSYELFVKLY CDRVELQPFG LVNLGNSCYA NAVLQCLAFT
360 370 380 390 400
RPLISYLIRG LHSKTCRKKS WCFVCEFEHL ILKARGGESP LSPIKILSKL
410 420 430 440 450
QKIGKHLGPG KEEDAHEFLR CAVDTMQSVF LKEAPAAGPF AEETTLVGLT
460 470 480 490 500
FGGYLHSKIK CMACLHKSER PELMMDLTVE IDGDIGSLEE ALAQFTAYEV
510 520 530 540 550
LDGENRYFCG RCKSYQKAKK KLMILEGPNI LTVVLKRFQS DNFGKLSKPI
560 570 580 590 600
HFPELLDISP YMSDPNHGDH PVYSLYAVVV HLDAMSTLFS GHYVCYIKTL
610 620 630 640 650
DGDWFKIDDS NVFPVQLETV LLEGAYMLLY ARDSPRPVSK NGGRKSKQRR
660 670 680 690 700
NLAAIPSRKG NKKQRDGDNN SLLPRVDWSS GSLSSMFSSS DTTSSCSTKD
710 720 730
SSGIENLSDY LFGGVEPVWK WDRHNKSQTF D
Length:731
Mass (Da):81,999
Last modified:March 1, 2001 - v1
Checksum:iD17844AC368057B5
GO
Isoform 2 (identifier: Q9FKP5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     421-448: CAVDTMQSVFLKEAPAAGPFAEETTLVG → MPGVLLIQCNLFFSKRLLQLVRLLKKLL
     449-731: Missing.

Note: May be due to a competing acceptor splice site. No experimental confirmation available.
Show »
Length:448
Mass (Da):50,543
Checksum:iC10389FE47E17BA5
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei421 – 44828CAVDT…TTLVG → MPGVLLIQCNLFFSKRLLQL VRLLKKLL in isoform 2. 1 PublicationVSP_029990Add
BLAST
Alternative sequencei449 – 731283Missing in isoform 2. 1 PublicationVSP_029991Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302667 mRNA. No translation available.
AB011479 Genomic DNA. Translation: BAB11567.1.
CP002688 Genomic DNA. Translation: AED98058.1.
RefSeqiNP_201348.1. NM_125943.1. [Q9FKP5-1]
UniGeneiAt.16943.

Genome annotation databases

EnsemblPlantsiAT5G65450.1; AT5G65450.1; AT5G65450. [Q9FKP5-1]
GeneIDi836670.
KEGGiath:AT5G65450.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302667 mRNA. No translation available.
AB011479 Genomic DNA. Translation: BAB11567.1.
CP002688 Genomic DNA. Translation: AED98058.1.
RefSeqiNP_201348.1. NM_125943.1. [Q9FKP5-1]
UniGeneiAt.16943.

3D structure databases

ProteinModelPortaliQ9FKP5.
SMRiQ9FKP5. Positions 330-630.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G65450.1.

Protein family/group databases

MEROPSiC19.A06.

Proteomic databases

PaxDbiQ9FKP5.
PRIDEiQ9FKP5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G65450.1; AT5G65450.1; AT5G65450. [Q9FKP5-1]
GeneIDi836670.
KEGGiath:AT5G65450.

Organism-specific databases

TAIRiAT5G65450.

Phylogenomic databases

eggNOGiCOG5533.
HOGENOMiHOG000242869.
InParanoidiQ9FKP5.
KOiK11855.
OMAiHEFLRCA.
PhylomeDBiQ9FKP5.

Enzyme and pathway databases

BioCyciARA:AT5G65450-MONOMER.

Miscellaneous databases

PROiQ9FKP5.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR002893. Znf_MYND.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF01753. zf-MYND. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS50235. USP_3. 1 hit.
PS01360. ZF_MYND_1. 1 hit.
PS50865. ZF_MYND_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
    Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
    Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), GENE FAMILY ORGANIZATION, NOMENCLATURE.
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones."
    Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.

Entry informationi

Entry nameiUBP17_ARATH
AccessioniPrimary (citable) accession number: Q9FKP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2001
Last modified: June 24, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.