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Q9FKL9 (XTH12_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable xyloglucan endotransglucosylase/hydrolase protein 12

Short name=At-XTH12
Short name=XTH-12
EC=2.4.1.207
Gene names
Name:XTH12
Ordered Locus Names:At5g57530
ORF Names:MUA2.10
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length285 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.

Catalytic activity

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Subcellular location

Secretedcell wall Probable. Secretedextracellular spaceapoplast Probable.

Tissue specificity

Root specific. Ref.4

Induction

Strongly down-regulated by abscisic acid (ABA). Ref.4

Post-translational modification

Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 16 family. XTH group 2 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 285260Probable xyloglucan endotransglucosylase/hydrolase protein 12
PRO_0000011812

Sites

Active site1011Nucleophile By similarity
Active site1051Proton donor By similarity

Amino acid modifications

Glycosylation1091N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q9FKL9 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 0544E8E568F5C543

FASTA28532,190
        10         20         30         40         50         60 
MAAFATKQSP LLLASLLILI GVATGSFYDS FDITWGAGRA NIFESGQLLT CTLDKTSGSG 

        70         80         90        100        110        120 
FQSKKEYLFG KIDMKIKLVP GNSAGTVTAY YLSSKGETWD EIDFEFLGNV TGQPYVIHTN 

       130        140        150        160        170        180 
VFTGGKGNRE MQFYLWFDPT ADFHTYTVLW NPLNIIFLVD GIPIRVFKNN EANGVAYPKS 

       190        200        210        220        230        240 
QPMKIYSSLW EADDWATQGG KVKTDWTNAP FSASYRSFND VDCCSRTSIW NWVTCNANSN 

       250        260        270        280 
SWMWTTLNSN QLGQLKWVQK DYMIYNYCTD FKRFPQGLPT ECNLN 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones."
Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S.
DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis."
Yokoyama R., Nishitani K.
Plant Cell Physiol. 42:1025-1033(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION.
[5]"The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
Rose J.K.C., Braam J., Fry S.C., Nishitani K.
Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB011482 Genomic DNA. Translation: BAB08788.1.
CP002688 Genomic DNA. Translation: AED96911.1.
AY057625 mRNA. Translation: AAL15256.1.
AY113025 mRNA. Translation: AAM47333.1.
RefSeqNP_200561.1. NM_125134.1.
UniGeneAt.26243.

3D structure databases

ProteinModelPortalQ9FKL9.
SMRQ9FKL9. Positions 26-282.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT5G57530.1-P.

Protein family/group databases

CAZyGH16. Glycoside Hydrolase Family 16.

Proteomic databases

PRIDEQ9FKL9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G57530.1; AT5G57530.1; AT5G57530.
GeneID835857.
KEGGath:AT5G57530.

Organism-specific databases

TAIRAT5G57530.

Phylogenomic databases

eggNOGCOG2273.
HOGENOMHOG000236368.
InParanoidQ9FKL9.
KOK08235.
OMARANIFES.
PhylomeDBQ9FKL9.

Enzyme and pathway databases

BioCycARA:AT5G57530-MONOMER.

Gene expression databases

GenevestigatorQ9FKL9.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFPIRSF005604. XET. 1 hit.
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameXTH12_ARATH
AccessionPrimary (citable) accession number: Q9FKL9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: March 1, 2001
Last modified: May 14, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names