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Q9FKL9

- XTH12_ARATH

UniProt

Q9FKL9 - XTH12_ARATH

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Protein

Probable xyloglucan endotransglucosylase/hydrolase protein 12

Gene

XTH12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues (By similarity).By similarity

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei101 – 1011NucleophilePROSITE-ProRule annotation
Active sitei105 – 1051Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

  1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
  2. xyloglucan:xyloglucosyl transferase activity Source: UniProtKB-EC
  3. xyloglucan endotransglucosylase activity Source: TAIR

GO - Biological processi

  1. cell wall organization Source: UniProtKB-KW
  2. xyloglucan metabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT5G57530-MONOMER.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable xyloglucan endotransglucosylase/hydrolase protein 12 (EC:2.4.1.207)
Short name:
At-XTH12
Short name:
XTH-12
Gene namesi
Name:XTH12
Ordered Locus Names:At5g57530
ORF Names:MUA2.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G57530.

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: UniProtKB-KW
  2. cell wall Source: TAIR
  3. cytoplasm Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 285260Probable xyloglucan endotransglucosylase/hydrolase protein 12PRO_0000011812Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi109 – 1091N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ9FKL9.

Expressioni

Tissue specificityi

Root specific.1 Publication

Inductioni

Strongly down-regulated by abscisic acid (ABA).1 Publication

Gene expression databases

GenevestigatoriQ9FKL9.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G57530.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9FKL9.
SMRiQ9FKL9. Positions 26-282.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2273.
HOGENOMiHOG000236368.
InParanoidiQ9FKL9.
KOiK08235.
OMAiRANIFES.
PhylomeDBiQ9FKL9.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FKL9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAFATKQSP LLLASLLILI GVATGSFYDS FDITWGAGRA NIFESGQLLT
60 70 80 90 100
CTLDKTSGSG FQSKKEYLFG KIDMKIKLVP GNSAGTVTAY YLSSKGETWD
110 120 130 140 150
EIDFEFLGNV TGQPYVIHTN VFTGGKGNRE MQFYLWFDPT ADFHTYTVLW
160 170 180 190 200
NPLNIIFLVD GIPIRVFKNN EANGVAYPKS QPMKIYSSLW EADDWATQGG
210 220 230 240 250
KVKTDWTNAP FSASYRSFND VDCCSRTSIW NWVTCNANSN SWMWTTLNSN
260 270 280
QLGQLKWVQK DYMIYNYCTD FKRFPQGLPT ECNLN
Length:285
Mass (Da):32,190
Last modified:March 1, 2001 - v1
Checksum:i0544E8E568F5C543
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011482 Genomic DNA. Translation: BAB08788.1.
CP002688 Genomic DNA. Translation: AED96911.1.
AY057625 mRNA. Translation: AAL15256.1.
AY113025 mRNA. Translation: AAM47333.1.
RefSeqiNP_200561.1. NM_125134.1.
UniGeneiAt.26243.

Genome annotation databases

EnsemblPlantsiAT5G57530.1; AT5G57530.1; AT5G57530.
GeneIDi835857.
KEGGiath:AT5G57530.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011482 Genomic DNA. Translation: BAB08788.1 .
CP002688 Genomic DNA. Translation: AED96911.1 .
AY057625 mRNA. Translation: AAL15256.1 .
AY113025 mRNA. Translation: AAM47333.1 .
RefSeqi NP_200561.1. NM_125134.1.
UniGenei At.26243.

3D structure databases

ProteinModelPortali Q9FKL9.
SMRi Q9FKL9. Positions 26-282.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT5G57530.1-P.

Protein family/group databases

CAZyi GH16. Glycoside Hydrolase Family 16.

Proteomic databases

PRIDEi Q9FKL9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G57530.1 ; AT5G57530.1 ; AT5G57530 .
GeneIDi 835857.
KEGGi ath:AT5G57530.

Organism-specific databases

TAIRi AT5G57530.

Phylogenomic databases

eggNOGi COG2273.
HOGENOMi HOG000236368.
InParanoidi Q9FKL9.
KOi K08235.
OMAi RANIFES.
PhylomeDBi Q9FKL9.

Enzyme and pathway databases

BioCyci ARA:AT5G57530-MONOMER.

Gene expression databases

Genevestigatori Q9FKL9.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view ]
Pfami PF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF005604. XET. 1 hit.
SUPFAMi SSF49899. SSF49899. 1 hit.
PROSITEi PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones."
    Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis."
    Yokoyama R., Nishitani K.
    Plant Cell Physiol. 42:1025-1033(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION.
  5. "The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
    Rose J.K.C., Braam J., Fry S.C., Nishitani K.
    Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.

Entry informationi

Entry nameiXTH12_ARATH
AccessioniPrimary (citable) accession number: Q9FKL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: March 1, 2001
Last modified: October 29, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3