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Protein

Probable xyloglucan endotransglucosylase/hydrolase protein 12

Gene

XTH12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues (By similarity).By similarity

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei101NucleophilePROSITE-ProRule annotation1
Active sitei105Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • cell wall biogenesis Source: InterPro
  • cell wall organization Source: UniProtKB-KW
  • xyloglucan metabolic process Source: TAIR

Keywordsi

Molecular functionGlycosidase, Hydrolase, Transferase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT5G57530-MONOMER
BRENDAi2.4.1.207 399

Protein family/group databases

CAZyiGH16 Glycoside Hydrolase Family 16

Names & Taxonomyi

Protein namesi
Recommended name:
Probable xyloglucan endotransglucosylase/hydrolase protein 12 (EC:2.4.1.207)
Short name:
At-XTH12
Short name:
XTH-12
Gene namesi
Name:XTH12
Ordered Locus Names:At5g57530
ORF Names:MUA2.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G57530
TAIRilocus:2174572 AT5G57530

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000001181226 – 285Probable xyloglucan endotransglucosylase/hydrolase protein 12Add BLAST260

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi109N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9FKL9

Expressioni

Tissue specificityi

Root specific.1 Publication

Inductioni

Strongly down-regulated by abscisic acid (ABA).1 Publication

Gene expression databases

ExpressionAtlasiQ9FKL9 baseline and differential
GenevisibleiQ9FKL9 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G57530.1

Structurei

3D structure databases

ProteinModelPortaliQ9FKL9
SMRiQ9FKL9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 215GH16PROSITE-ProRule annotationAdd BLAST190

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IFD4 Eukaryota
COG2273 LUCA
HOGENOMiHOG000236368
InParanoidiQ9FKL9
KOiK08235
OMAiWWEDSAY
OrthoDBiEOG09360GVA
PhylomeDBiQ9FKL9

Family and domain databases

InterProiView protein in InterPro
IPR013320 ConA-like_dom_sf
IPR000757 GH16
IPR008263 GH16_AS
IPR010713 XET_C
IPR016455 XTH
PfamiView protein in Pfam
PF00722 Glyco_hydro_16, 1 hit
PF06955 XET_C, 1 hit
PIRSFiPIRSF005604 XET, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS01034 GH16_1, 1 hit
PS51762 GH16_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FKL9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAFATKQSP LLLASLLILI GVATGSFYDS FDITWGAGRA NIFESGQLLT
60 70 80 90 100
CTLDKTSGSG FQSKKEYLFG KIDMKIKLVP GNSAGTVTAY YLSSKGETWD
110 120 130 140 150
EIDFEFLGNV TGQPYVIHTN VFTGGKGNRE MQFYLWFDPT ADFHTYTVLW
160 170 180 190 200
NPLNIIFLVD GIPIRVFKNN EANGVAYPKS QPMKIYSSLW EADDWATQGG
210 220 230 240 250
KVKTDWTNAP FSASYRSFND VDCCSRTSIW NWVTCNANSN SWMWTTLNSN
260 270 280
QLGQLKWVQK DYMIYNYCTD FKRFPQGLPT ECNLN
Length:285
Mass (Da):32,190
Last modified:March 1, 2001 - v1
Checksum:i0544E8E568F5C543
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011482 Genomic DNA Translation: BAB08788.1
CP002688 Genomic DNA Translation: AED96911.1
AY057625 mRNA Translation: AAL15256.1
AY113025 mRNA Translation: AAM47333.1
RefSeqiNP_200561.1, NM_125134.2
UniGeneiAt.26243

Genome annotation databases

EnsemblPlantsiAT5G57530.1; AT5G57530.1; AT5G57530
GeneIDi835857
GrameneiAT5G57530.1; AT5G57530.1; AT5G57530
KEGGiath:AT5G57530

Similar proteinsi

Entry informationi

Entry nameiXTH12_ARATH
AccessioniPrimary (citable) accession number: Q9FKL9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: March 1, 2001
Last modified: April 25, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health