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Protein

Putative xyloglucan endotransglucosylase/hydrolase protein 13

Gene

XTH13

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

May catalyze xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues (By similarity).By similarity

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei100 – 1001NucleophilePROSITE-ProRule annotation
Active sitei104 – 1041Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

  1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
  2. xyloglucan:xyloglucosyl transferase activity Source: UniProtKB-EC
  3. xyloglucan endotransglucosylase activity Source: TAIR

GO - Biological processi

  1. cell wall organization Source: UniProtKB-KW
  2. xyloglucan metabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT5G57540-MONOMER.
BRENDAi2.4.1.207. 399.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative xyloglucan endotransglucosylase/hydrolase protein 13 (EC:2.4.1.207)
Short name:
At-XTH13
Short name:
XTH-13
Gene namesi
Name:XTH13
Ordered Locus Names:At5g57540
ORF Names:MUA2.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G57540.

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: UniProtKB-SubCell
  2. cell wall Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 284260Putative xyloglucan endotransglucosylase/hydrolase protein 13PRO_0000011813Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi108 – 1081N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ9FKL8.

Expressioni

Gene expression databases

GenevestigatoriQ9FKL8.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G57540.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9FKL8.
SMRiQ9FKL8. Positions 25-281.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2273.
HOGENOMiHOG000236368.
InParanoidiQ9FKL8.
KOiK08235.
OMAiVAYAGNM.
PhylomeDBiQ9FKL8.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR008264. Beta_glucanase.
IPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
PRINTSiPR00737. GLHYDRLASE16.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FKL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAFTTKQSL LLLSLLLLIS LSAGSFYDNF DITWGNGRAN IVESGQLLTC
60 70 80 90 100
TLDKISGSGF QSKKEYLFGK IDMKMKLVAG NSAGTVTAYY LSSKGETWDE
110 120 130 140 150
IDFEFLGNVT GQPYVLHTNV FTGGKGNREM QFYLWFDPTA DFHTYTVLWN
160 170 180 190 200
PLNIIFLVDG IPIRVFKNNE ANGVAYPKSQ PMKIYSSLWE ADDWATQGGK
210 220 230 240 250
VKTDWTNAPF SASYKSFNDV DCCSRTSLLN WVTCNANSNS WMWTTLNSNQ
260 270 280
YGQMKWVQDD YMIYNYCTDF KRFPQGLPTE CNLN
Length:284
Mass (Da):32,175
Last modified:February 28, 2001 - v1
Checksum:i87EA45CC0DC628EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011482 Genomic DNA. Translation: BAB08789.1.
CP002688 Genomic DNA. Translation: AED96913.1.
RefSeqiNP_200562.1. NM_125135.1.
UniGeneiAt.55604.

Genome annotation databases

EnsemblPlantsiAT5G57540.1; AT5G57540.1; AT5G57540.
GeneIDi835858.
KEGGiath:AT5G57540.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011482 Genomic DNA. Translation: BAB08789.1.
CP002688 Genomic DNA. Translation: AED96913.1.
RefSeqiNP_200562.1. NM_125135.1.
UniGeneiAt.55604.

3D structure databases

ProteinModelPortaliQ9FKL8.
SMRiQ9FKL8. Positions 25-281.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G57540.1-P.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Proteomic databases

PRIDEiQ9FKL8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G57540.1; AT5G57540.1; AT5G57540.
GeneIDi835858.
KEGGiath:AT5G57540.

Organism-specific databases

TAIRiAT5G57540.

Phylogenomic databases

eggNOGiCOG2273.
HOGENOMiHOG000236368.
InParanoidiQ9FKL8.
KOiK08235.
OMAiVAYAGNM.
PhylomeDBiQ9FKL8.

Enzyme and pathway databases

BioCyciARA:AT5G57540-MONOMER.
BRENDAi2.4.1.207. 399.

Gene expression databases

GenevestigatoriQ9FKL8.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR008264. Beta_glucanase.
IPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
PRINTSiPR00737. GLHYDRLASE16.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones."
    Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 5:131-145(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
    Rose J.K.C., Braam J., Fry S.C., Nishitani K.
    Plant Cell Physiol. 43:1421-1435(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.

Entry informationi

Entry nameiXTH13_ARATH
AccessioniPrimary (citable) accession number: Q9FKL8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 27, 2003
Last sequence update: February 28, 2001
Last modified: March 31, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.