Q9FKL8 (XTH13_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative xyloglucan endotransglucosylase/hydrolase protein 13
Short name=At-XTH13
Short name=XTH-13
EC=2.4.1.207

Gene names

Name:	XTH13
Ordered Locus Names:	At5g57540
ORF Names:	MUA2.11

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May catalyze xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.
Catalytic activity	Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.
Subcellular location	Secreted › cell wall Probable. Secreted › extracellular space › apoplast Probable.
Post-translational modification	Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 16 family. XTH group 2 subfamily.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Apoplast Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	xyloglucan metabolic process Inferred from direct assay PubMed 20732879. Source: TAIR
Cellular_component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro xyloglucan endotransglucosylase activity Inferred from direct assay PubMed 20732879. Source: TAIR xyloglucan:xyloglucosyl transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 284	260	Putative xyloglucan endotransglucosylase/hydrolase protein 13		PRO_0000011813
Sites
	Active site	100	1	Nucleophile By similarity
	Active site	104	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	108	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9FKL8 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 87EA45CC0DC628EE	FASTA	284	32,175
	10 20 30 40 50 60 MAAFTTKQSL LLLSLLLLIS LSAGSFYDNF DITWGNGRAN IVESGQLLTC TLDKISGSGF 70 80 90 100 110 120 QSKKEYLFGK IDMKMKLVAG NSAGTVTAYY LSSKGETWDE IDFEFLGNVT GQPYVLHTNV 130 140 150 160 170 180 FTGGKGNREM QFYLWFDPTA DFHTYTVLWN PLNIIFLVDG IPIRVFKNNE ANGVAYPKSQ 190 200 210 220 230 240 PMKIYSSLWE ADDWATQGGK VKTDWTNAPF SASYKSFNDV DCCSRTSLLN WVTCNANSNS 250 260 270 280 WMWTTLNSNQ YGQMKWVQDD YMIYNYCTDF KRFPQGLPTE CNLN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones." Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S. DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature." Rose J.K.C., Braam J., Fry S.C., Nishitani K. Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE.
+	Additional computationally mapped references.

Web resources

XTH-World

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB011482 Genomic DNA. Translation: BAB08789.1. CP002688 Genomic DNA. Translation: AED96913.1.
IPI	IPI00529293.
RefSeq	NP_200562.1. NM_125135.1.
UniGene	At.55604.
3D structure databases
ProteinModelPortal	Q9FKL8.
SMR	Q9FKL8. Positions 25-281.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT5G57540.1-P.
Protein family/group databases
CAZy	GH16. Glycoside Hydrolase Family 16.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G57540.1; AT5G57540.1; AT5G57540.
GeneID	835858.
KEGG	ath:AT5G57540.
Organism-specific databases
TAIR	At5g57540.
Phylogenomic databases
eggNOG	COG2273.
HOGENOM	HOG000236368.
InParanoid	Q9FKL8.
KO	K08235.
OMA	DNFDITW.
PhylomeDB	Q9FKL8.
ProtClustDB	CLSN2685868.
Gene expression databases
Genevestigator	Q9FKL8.
GermOnline	AT5G57540. Arabidopsis thaliana.
Family and domain databases
Gene3D	2.60.120.200. 1 hit.
InterPro	IPR008264. Beta_glucanase. IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. IPR000757. Glyco_hydro_16. IPR008263. Glycoside_hydrolase_16_AS. IPR016455. XET. IPR010713. XET_C. [Graphical view]
Pfam	PF00722. Glyco_hydro_16. 1 hit. PF06955. XET_C. 1 hit. [Graphical view]
PIRSF	PIRSF005604. XET. 1 hit.
PRINTS	PR00737. GLHYDRLASE16.
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS01034. GLYCOSYL_HYDROL_F16. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XTH13_ARATH

Accession

Primary (citable) accession number: Q9FKL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program