Reviewed, UniProtKB/Swiss-Prot Q9FKK7 (XYLA_ARATH)

Names and origin

Protein names

Recommended name:
    Xylose isomerase
    EC=5.3.1.5

Gene names

Name:	XYLA
Ordered Locus Names:	At5g57655
ORF Names:	MUA2.25

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-xylose = D-xylulose.
Cofactor	Binds 2 manganese ions per subunit By similarity.
Sequence similarities	Belongs to the xylose isomerase family.
Sequence caution	The sequence BAB08802.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Pentose shunt Xylose metabolism
Coding sequence diversity	Alternative splicing
Ligand	Magnesium Manganese Metal-binding
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	D-xylose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from direct assay. Source: TAIR plasma membrane Inferred from direct assay. Source: TAIR vacuole Inferred from direct assay. Source: TAIR
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW xylose isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9FKK7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 477	477	Xylose isomerase		PRO_0000195824
Sites
	Active site	142	1	By similarity
	Metal binding	273	1	Manganese 1 By similarity
	Metal binding	309	1	Manganese 1 By similarity
	Metal binding	309	1	Manganese 2 By similarity
	Metal binding	312	1	Manganese 2 By similarity
	Metal binding	337	1	Manganese 1 By similarity
	Metal binding	348	1	Manganese 2 By similarity
	Metal binding	350	1	Manganese 2 By similarity
	Metal binding	380	1	Manganese 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 29, 2007. Version 2. Checksum: 4C89078F3179AE0C	FASTA	477	53,720
	10 20 30 40 50 60 MKKVEFFMLL LCFIAASSLV SADPPTCPAD LGGKCSDSDD WQGDFFPEIP KIKYEGPSSK 70 80 90 100 110 120 NPLAYRWYNA EEEILGKKMK DWFRFSVAFW HTFRGTGGDP FGAATKYWPW EDGTNSVSMA 130 140 150 160 170 180 KRRMRANFEF LKKLGVDWWC FHDRDIAPDG TTLEESNKNL DEVIELAKEL QKGSKIKPLW 190 200 210 220 230 240 GTAQLFLHPR YMHGGATSSE VGVYAYAAAQ VKKAMEVTHY LGGENYVFWG GREGYQTLLN 250 260 270 280 290 300 TDMGRELDHL ARFFEAAVAY KKKIGFKGTL LIEPKPQEPT KHQYDWDAAT AANFLRKYGL 310 320 330 340 350 360 IDEFKLNIEC NHATLSGHTC HHELETARIN GLLGNIDANT GDAQTGWDTD QFLTDVGEAT 370 380 390 400 410 420 MVMMSVIKNG GIAPGGFNFD AKLRRESTDV EDLFIAHISG MDTMARGLRN AVKILEEGSL 430 440 450 460 470 SELVRKRYAT WDSELGKQIE EGKADFEYLE KKAKEFGEPK VSSAKQELAE MIFQSAM

References

[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones." Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S. DNA Res. 5:131-145(1998) [PubMed: 9679202] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB011482 Genomic DNA. Translation: BAB08802.1. Sequence problems. AY136469 mRNA. Translation: AAM97134.1. BT006607 mRNA. Translation: AAP31951.1.
IPI	IPI00535514.
RefSeq	NP_568861.3.
UniGene	At.20353 At.67777 Rra.844 Rsa.4021
3D structure databases
SMR	Q9FKK7. Positions 44-475.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9FKK7.
Proteomic databases
PRIDE	Q9FKK7.
Genome annotation databases
GeneID	835871.
GenomeReviews	Gene locus AT5G57655 in contig BA000015_GR.
KEGG	ath:AT5G57655.
NMPDR	fig|3702.1.peg.27719.
Organism-specific databases
TAIR	At5g57655.
Phylogenomic databases
HOGENOM	HBG297199.
InParanoid	Q9FKK7.
OMA	LLGWDTD.
PhylomeDB	Q9FKK7.
Enzyme and pathway databases
BRENDA	5.3.1.5. 302.
Gene expression databases
Genevestigator	Q9FKK7.
Family and domain databases
InterPro	IPR013022. Xyl_isomerase-like_TIM-brl. IPR012307. Xyl_isomerase_TIM-brl. IPR013452. Xylose_isom_bac. IPR001998. Xylose_isomerase. [Graphical view]
Pfam	PF01261. AP_endonuc_2. 1 hit. [Graphical view]
PRINTS	PR00688. XYLOSISMRASE.
TIGRFAMs	TIGR02630. xylose_isom_A. 1 hit.
PROSITE	PS51415. XYLOSE_ISOMERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYLA_ARATH

Accession

Primary (citable) accession number: Q9FKK7
Secondary accession number(s): Q8L759

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	May 29, 2007
Last modified:	February 9, 2010
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)