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Q9FKG3 (PFKA4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent 6-phosphofructokinase 4, chloroplastic

Short name=ATP-PFK 4
Short name=Phosphofructokinase 4
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase 4
Gene names
Name:PFK4
Ordered Locus Names:At5g61580
ORF Names:K11J9.3
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length530 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity. HAMAP-Rule MF_03186

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. Ref.4

Cofactor

Magnesium By similarity. HAMAP-Rule MF_03186

Enzyme regulation

Allosterically activated by AMP By similarity. HAMAP-Rule MF_03186

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_03186

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_03186

Subcellular location

Plastidchloroplast Ref.4.

Tissue specificity

Expressed in leaves, stems and flowers. Ref.4

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9FKG3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9FKG3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     476-476: Missing.
Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5454Chloroplast Potential
Chain55 – 530476ATP-dependent 6-phosphofructokinase 4, chloroplastic HAMAP-Rule MF_03186
PRO_0000330771

Regions

Nucleotide binding215 – 2162ATP By similarity
Nucleotide binding240 – 2434ATP By similarity
Region269 – 2713Substrate binding By similarity
Region314 – 3163Substrate binding By similarity
Region427 – 4304Substrate binding By similarity

Sites

Active site2711Proton acceptor By similarity
Binding site1521ATP; via amide nitrogen By similarity
Binding site3701Substrate By similarity
Site2421Important for substrate specificity; cannot use PPi as phosphoryl donor By similarity

Amino acid modifications

Modified residue1211Phosphoserine By similarity

Natural variations

Alternative sequence4761Missing in isoform 2.
VSP_033114

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 7CF9AC8460F44DF4

FASTA53058,467
        10         20         30         40         50         60 
MEASISFLGS TKPNISLFNP SSNVLPRRDF PLPALKLKKV SVLPRILHQK RLIRAQCSDG 

        70         80         90        100        110        120 
FKPEEDDGFV LEDVPHLTKF LPDLPSYPNP LKESQAYAIV KRTFVSSEDV VAQNIVVQKG 

       130        140        150        160        170        180 
SKRGVHFRRA GPRERVYFRS DEVKACIVTC GGLCPGINTV IREIVCGLNN MYGVNNILGI 

       190        200        210        220        230        240 
QGGYRGFYSK NTMNLTPKVV NDIHKRGGTF LQTSRGGHDT AKIVDNIQDR GINQVYIIGG 

       250        260        270        280        290        300 
GGTQKGAEKI YEEVERRGLQ VAVSGIPKTI DNDIAVIDKS FGFDTAVEEA QRAINAAHVE 

       310        320        330        340        350        360 
VESVENGVGI VKLMGRYSGF IAMIATLANR DVDCCLIPES PFFLEGKGGL FEFIEERLKE 

       370        380        390        400        410        420 
NRHMVIVIAE GAGQDYVAQS MRASETKDAS GNRLLLDVGL WLTQQIKDHF TNVRKMMINM 

       430        440        450        460        470        480 
KYIDPTYMIR AIPSNASDNV YCTLLAQSAV HGAMAGYSGF TVGPVNSRHA YIPISQVTEV 

       490        500        510        520        530 
TNTVKLTDRM WARLLASTNQ PSFLTGEGAL QNVIDMETQE KIDNMKISSI 

« Hide

Isoform 2 [UniParc].

Checksum: 241B3E01D4D4D85D
Show »

FASTA52958,339

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[4]"Characterisation of the ATP-dependent phosphofructokinase gene family from Arabidopsis thaliana."
Mustroph A., Sonnewald U., Biemelt S.
FEBS Lett. 581:2401-2410(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: CATALYTIC ACTIVITY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB012239 Genomic DNA. Translation: BAB09002.1.
CP002688 Genomic DNA. Translation: AED97492.1.
CP002688 Genomic DNA. Translation: AED97493.1.
AY099694 mRNA. Translation: AAM20545.1.
AY128873 mRNA. Translation: AAM91273.1.
RefSeqNP_001032120.1. NM_001037043.1. [Q9FKG3-2]
NP_200966.2. NM_125551.3. [Q9FKG3-1]
UniGeneAt.29096.

3D structure databases

ProteinModelPortalQ9FKG3.
SMRQ9FKG3. Positions 87-494.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid21523. 1 interaction.

Proteomic databases

PaxDbQ9FKG3.
PRIDEQ9FKG3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G61580.1; AT5G61580.1; AT5G61580. [Q9FKG3-1]
GeneID836279.
KEGGath:AT5G61580.

Organism-specific databases

TAIRAT5G61580.

Phylogenomic databases

eggNOGCOG0205.
HOGENOMHOG000016186.
InParanoidQ9FKG3.
KOK00850.
OMAWLTQQIK.
PhylomeDBQ9FKG3.

Enzyme and pathway databases

BioCycARA:AT5G61580-MONOMER.
ARA:GQT-2054-MONOMER.
UniPathwayUPA00109; UER00182.

Gene expression databases

GenevestigatorQ9FKG3.

Family and domain databases

HAMAPMF_01981. Phosphofructokinase_II_X.
InterProIPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFruKinase_TP0108.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
ProtoNetSearch...

Entry information

Entry namePFKA4_ARATH
AccessionPrimary (citable) accession number: Q9FKG3
Secondary accession number(s): Q2V2W5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names