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Q9FKG3

- PFKA4_ARATH

UniProt

Q9FKG3 - PFKA4_ARATH

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Protein

ATP-dependent 6-phosphofructokinase 4, chloroplastic

Gene
PFK4, At5g61580, K11J9.3
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.1 Publication

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by AMP By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei152 – 1521ATP; via amide nitrogen By similarity
Sitei242 – 2421Important for substrate specificity; cannot use PPi as phosphoryl donor By similarity
Active sitei271 – 2711Proton acceptor By similarity
Binding sitei370 – 3701Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi215 – 2162ATP By similarity
Nucleotide bindingi240 – 2434ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: TAIR
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. carbohydrate phosphorylation Source: GOC
  2. fructose 6-phosphate metabolic process Source: InterPro
  3. glycolytic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G61580-MONOMER.
ARA:GQT-2054-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 4, chloroplastic (EC:2.7.1.11)
Short name:
ATP-PFK 4
Short name:
Phosphofructokinase 4
Alternative name(s):
Phosphohexokinase 4
Gene namesi
Name:PFK4
Ordered Locus Names:At5g61580
ORF Names:K11J9.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G61580.

Subcellular locationi

Plastidchloroplast 1 Publication

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
  2. chloroplast Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5454Chloroplast Reviewed predictionAdd
BLAST
Chaini55 – 530476ATP-dependent 6-phosphofructokinase 4, chloroplasticUniRule annotationPRO_0000330771Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei121 – 1211Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9FKG3.
PRIDEiQ9FKG3.

Expressioni

Tissue specificityi

Expressed in leaves, stems and flowers.1 Publication

Gene expression databases

GenevestigatoriQ9FKG3.

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

BioGridi21523. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9FKG3.
SMRiQ9FKG3. Positions 87-494.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni269 – 2713Substrate binding By similarity
Regioni314 – 3163Substrate binding By similarity
Regioni427 – 4304Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000016186.
InParanoidiQ9FKG3.
KOiK00850.
OMAiWLTQQIK.
PhylomeDBiQ9FKG3.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X.
InterProiIPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFruKinase_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9FKG3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEASISFLGS TKPNISLFNP SSNVLPRRDF PLPALKLKKV SVLPRILHQK    50
RLIRAQCSDG FKPEEDDGFV LEDVPHLTKF LPDLPSYPNP LKESQAYAIV 100
KRTFVSSEDV VAQNIVVQKG SKRGVHFRRA GPRERVYFRS DEVKACIVTC 150
GGLCPGINTV IREIVCGLNN MYGVNNILGI QGGYRGFYSK NTMNLTPKVV 200
NDIHKRGGTF LQTSRGGHDT AKIVDNIQDR GINQVYIIGG GGTQKGAEKI 250
YEEVERRGLQ VAVSGIPKTI DNDIAVIDKS FGFDTAVEEA QRAINAAHVE 300
VESVENGVGI VKLMGRYSGF IAMIATLANR DVDCCLIPES PFFLEGKGGL 350
FEFIEERLKE NRHMVIVIAE GAGQDYVAQS MRASETKDAS GNRLLLDVGL 400
WLTQQIKDHF TNVRKMMINM KYIDPTYMIR AIPSNASDNV YCTLLAQSAV 450
HGAMAGYSGF TVGPVNSRHA YIPISQVTEV TNTVKLTDRM WARLLASTNQ 500
PSFLTGEGAL QNVIDMETQE KIDNMKISSI 530
Length:530
Mass (Da):58,467
Last modified:March 1, 2001 - v1
Checksum:i7CF9AC8460F44DF4
GO
Isoform 2 (identifier: Q9FKG3-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     476-476: Missing.

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:529
Mass (Da):58,339
Checksum:i241B3E01D4D4D85D
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei476 – 4761Missing in isoform 2. VSP_033114

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB012239 Genomic DNA. Translation: BAB09002.1.
CP002688 Genomic DNA. Translation: AED97492.1.
CP002688 Genomic DNA. Translation: AED97493.1.
AY099694 mRNA. Translation: AAM20545.1.
AY128873 mRNA. Translation: AAM91273.1.
RefSeqiNP_001032120.1. NM_001037043.1. [Q9FKG3-2]
NP_200966.2. NM_125551.3. [Q9FKG3-1]
UniGeneiAt.29096.

Genome annotation databases

EnsemblPlantsiAT5G61580.1; AT5G61580.1; AT5G61580. [Q9FKG3-1]
GeneIDi836279.
KEGGiath:AT5G61580.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB012239 Genomic DNA. Translation: BAB09002.1 .
CP002688 Genomic DNA. Translation: AED97492.1 .
CP002688 Genomic DNA. Translation: AED97493.1 .
AY099694 mRNA. Translation: AAM20545.1 .
AY128873 mRNA. Translation: AAM91273.1 .
RefSeqi NP_001032120.1. NM_001037043.1. [Q9FKG3-2 ]
NP_200966.2. NM_125551.3. [Q9FKG3-1 ]
UniGenei At.29096.

3D structure databases

ProteinModelPortali Q9FKG3.
SMRi Q9FKG3. Positions 87-494.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 21523. 1 interaction.

Proteomic databases

PaxDbi Q9FKG3.
PRIDEi Q9FKG3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G61580.1 ; AT5G61580.1 ; AT5G61580 . [Q9FKG3-1 ]
GeneIDi 836279.
KEGGi ath:AT5G61580.

Organism-specific databases

TAIRi AT5G61580.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000016186.
InParanoidi Q9FKG3.
KOi K00850.
OMAi WLTQQIK.
PhylomeDBi Q9FKG3.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci ARA:AT5G61580-MONOMER.
ARA:GQT-2054-MONOMER.

Gene expression databases

Genevestigatori Q9FKG3.

Family and domain databases

HAMAPi MF_01981. Phosphofructokinase_II_X.
InterProi IPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFruKinase_TP0108.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
    Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
    DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Characterisation of the ATP-dependent phosphofructokinase gene family from Arabidopsis thaliana."
    Mustroph A., Sonnewald U., Biemelt S.
    FEBS Lett. 581:2401-2410(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiPFKA4_ARATH
AccessioniPrimary (citable) accession number: Q9FKG3
Secondary accession number(s): Q2V2W5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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