Q9FKG3 (K6PF4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phosphofructokinase 4, chloroplastic
Short name=Phosphofructokinase 4
EC=2.7.1.11

Alternative name(s):
Phosphohexokinase 4

Gene names

Name:	PFK4
Ordered Locus Names:	At5g61580
ORF Names:	K11J9.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. Ref.4
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Subcellular location	Plastid › chloroplast Ref.4.
Tissue specificity	Expressed in leaves, stems and flowers. Ref.4
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from direct assay Ref.4. Source: TAIR
Cellular_component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro chloroplast Inferred from direct assay Ref.4. Source: TAIR
Molecular_function	6-phosphofructokinase activity Inferred from direct assay Ref.4. Source: TAIR ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9FKG3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9FKG3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     476-476: Missing.

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 54	54	Chloroplast Potential
	Chain	55 – 530	476	6-phosphofructokinase 4, chloroplastic		PRO_0000330771
Regions
	Nucleotide binding	162 – 166	5	ATP By similarity
	Nucleotide binding	298 – 302	5	ATP By similarity
	Nucleotide binding	316 – 333	18	ATP By similarity
Sites
	Active site	271	1	Proton acceptor By similarity
Natural variations
	Alternative sequence	476	1	Missing in isoform 2.		VSP_033114

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 7CF9AC8460F44DF4	FASTA	530	58,467
	10 20 30 40 50 60 MEASISFLGS TKPNISLFNP SSNVLPRRDF PLPALKLKKV SVLPRILHQK RLIRAQCSDG 70 80 90 100 110 120 FKPEEDDGFV LEDVPHLTKF LPDLPSYPNP LKESQAYAIV KRTFVSSEDV VAQNIVVQKG 130 140 150 160 170 180 SKRGVHFRRA GPRERVYFRS DEVKACIVTC GGLCPGINTV IREIVCGLNN MYGVNNILGI 190 200 210 220 230 240 QGGYRGFYSK NTMNLTPKVV NDIHKRGGTF LQTSRGGHDT AKIVDNIQDR GINQVYIIGG 250 260 270 280 290 300 GGTQKGAEKI YEEVERRGLQ VAVSGIPKTI DNDIAVIDKS FGFDTAVEEA QRAINAAHVE 310 320 330 340 350 360 VESVENGVGI VKLMGRYSGF IAMIATLANR DVDCCLIPES PFFLEGKGGL FEFIEERLKE 370 380 390 400 410 420 NRHMVIVIAE GAGQDYVAQS MRASETKDAS GNRLLLDVGL WLTQQIKDHF TNVRKMMINM 430 440 450 460 470 480 KYIDPTYMIR AIPSNASDNV YCTLLAQSAV HGAMAGYSGF TVGPVNSRHA YIPISQVTEV 490 500 510 520 530 TNTVKLTDRM WARLLASTNQ PSFLTGEGAL QNVIDMETQE KIDNMKISSI
	Isoform 2 [UniParc]. Checksum: 241B3E01D4D4D85D	FASTA	529	58,339
	10 20 30 40 50 60 MEASISFLGS TKPNISLFNP SSNVLPRRDF PLPALKLKKV SVLPRILHQK RLIRAQCSDG 70 80 90 100 110 120 FKPEEDDGFV LEDVPHLTKF LPDLPSYPNP LKESQAYAIV KRTFVSSEDV VAQNIVVQKG 130 140 150 160 170 180 SKRGVHFRRA GPRERVYFRS DEVKACIVTC GGLCPGINTV IREIVCGLNN MYGVNNILGI 190 200 210 220 230 240 QGGYRGFYSK NTMNLTPKVV NDIHKRGGTF LQTSRGGHDT AKIVDNIQDR GINQVYIIGG 250 260 270 280 290 300 GGTQKGAEKI YEEVERRGLQ VAVSGIPKTI DNDIAVIDKS FGFDTAVEEA QRAINAAHVE 310 320 330 340 350 360 VESVENGVGI VKLMGRYSGF IAMIATLANR DVDCCLIPES PFFLEGKGGL FEFIEERLKE 370 380 390 400 410 420 NRHMVIVIAE GAGQDYVAQS MRASETKDAS GNRLLLDVGL WLTQQIKDHF TNVRKMMINM 430 440 450 460 470 480 KYIDPTYMIR AIPSNASDNV YCTLLAQSAV HGAMAGYSGF TVGPVNSRHA YIPISVTEVT 490 500 510 520 NTVKLTDRMW ARLLASTNQP SFLTGEGALQ NVIDMETQEK IDNMKISSI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones." Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S. DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[4]	"Characterisation of the ATP-dependent phosphofructokinase gene family from Arabidopsis thaliana." Mustroph A., Sonnewald U., Biemelt S. FEBS Lett. 581:2401-2410(2007) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB012239 Genomic DNA. Translation: BAB09002.1. CP002688 Genomic DNA. Translation: AED97492.1. CP002688 Genomic DNA. Translation: AED97493.1. AY099694 mRNA. Translation: AAM20545.1. AY128873 mRNA. Translation: AAM91273.1.
IPI	IPI00517810. IPI00657462.
RefSeq	NP_001032120.1. NM_001037043.1. NP_200966.2. NM_125551.3.
UniGene	At.29096.
3D structure databases
HSSP	HSSP built from PDB template 3PFK based on UniProtKB P00512.
ProteinModelPortal	Q9FKG3.
SMR	Q9FKG3. Positions 87-494.
ModBase	Search...
Proteomic databases
PaxDb	Q9FKG3.
PRIDE	Q9FKG3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G61580.1; AT5G61580.1; AT5G61580.
GeneID	836279.
KEGG	ath:AT5G61580.
Organism-specific databases
TAIR	At5g61580.
Phylogenomic databases
eggNOG	COG0205.
HOGENOM	HOG000016186.
InParanoid	Q9FKG3.
KO	K00850.
OMA	LTDRMWA.
PhylomeDB	Q9FKG3.
ProtClustDB	PLN02564.
Enzyme and pathway databases
UniPathway	UPA00109; UER00182.
Gene expression databases
Genevestigator	Q9FKG3.
Family and domain databases
InterPro	IPR022953. Phosphofructokinase. IPR000023. Phosphofructokinase_dom. IPR012004. PyroP-dep_PFruKinase_TP0108. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

K6PF4_ARATH

Accession

Primary (citable) accession number: Q9FKG3
Secondary accession number(s): Q2V2W5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program