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Protein

ATP-dependent 6-phosphofructokinase 4, chloroplastic

Gene

PFK4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by AMP.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei152 – 1521ATP; via amide nitrogenUniRule annotation
Sitei242 – 2421Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Active sitei271 – 2711Proton acceptorUniRule annotation
Binding sitei370 – 3701SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi215 – 2162ATPUniRule annotation
Nucleotide bindingi240 – 2434ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: TAIR
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. carbohydrate phosphorylation Source: GOC
  2. fructose 6-phosphate metabolic process Source: InterPro
  3. glycolytic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G61580-MONOMER.
ARA:GQT-2054-MONOMER.
BRENDAi2.7.1.11. 399.
ReactomeiREACT_293115. Glycolysis.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 4, chloroplasticUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFK 4UniRule annotation
Short name:
Phosphofructokinase 4UniRule annotation
Alternative name(s):
Phosphohexokinase 4UniRule annotation
Gene namesi
Name:PFK4UniRule annotation
Ordered Locus Names:At5g61580
ORF Names:K11J9.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G61580.

Subcellular locationi

Plastidchloroplast 1 Publication

GO - Cellular componenti

  1. chloroplast Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5454ChloroplastSequence AnalysisAdd
BLAST
Chaini55 – 530476ATP-dependent 6-phosphofructokinase 4, chloroplasticPRO_0000330771Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei121 – 1211PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9FKG3.
PRIDEiQ9FKG3.

Expressioni

Tissue specificityi

Expressed in leaves, stems and flowers.1 Publication

Gene expression databases

GenevestigatoriQ9FKG3.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

BioGridi21523. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9FKG3.
SMRiQ9FKG3. Positions 87-494.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni269 – 2713Substrate bindingUniRule annotation
Regioni314 – 3163Substrate bindingUniRule annotation
Regioni427 – 4304Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000016186.
InParanoidiQ9FKG3.
KOiK00850.
OMAiIVVQKGS.
PhylomeDBiQ9FKG3.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X.
InterProiIPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9FKG3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEASISFLGS TKPNISLFNP SSNVLPRRDF PLPALKLKKV SVLPRILHQK
60 70 80 90 100
RLIRAQCSDG FKPEEDDGFV LEDVPHLTKF LPDLPSYPNP LKESQAYAIV
110 120 130 140 150
KRTFVSSEDV VAQNIVVQKG SKRGVHFRRA GPRERVYFRS DEVKACIVTC
160 170 180 190 200
GGLCPGINTV IREIVCGLNN MYGVNNILGI QGGYRGFYSK NTMNLTPKVV
210 220 230 240 250
NDIHKRGGTF LQTSRGGHDT AKIVDNIQDR GINQVYIIGG GGTQKGAEKI
260 270 280 290 300
YEEVERRGLQ VAVSGIPKTI DNDIAVIDKS FGFDTAVEEA QRAINAAHVE
310 320 330 340 350
VESVENGVGI VKLMGRYSGF IAMIATLANR DVDCCLIPES PFFLEGKGGL
360 370 380 390 400
FEFIEERLKE NRHMVIVIAE GAGQDYVAQS MRASETKDAS GNRLLLDVGL
410 420 430 440 450
WLTQQIKDHF TNVRKMMINM KYIDPTYMIR AIPSNASDNV YCTLLAQSAV
460 470 480 490 500
HGAMAGYSGF TVGPVNSRHA YIPISQVTEV TNTVKLTDRM WARLLASTNQ
510 520 530
PSFLTGEGAL QNVIDMETQE KIDNMKISSI
Length:530
Mass (Da):58,467
Last modified:March 1, 2001 - v1
Checksum:i7CF9AC8460F44DF4
GO
Isoform 2 (identifier: Q9FKG3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     476-476: Missing.

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:529
Mass (Da):58,339
Checksum:i241B3E01D4D4D85D
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei476 – 4761Missing in isoform 2. CuratedVSP_033114

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012239 Genomic DNA. Translation: BAB09002.1.
CP002688 Genomic DNA. Translation: AED97492.1.
CP002688 Genomic DNA. Translation: AED97493.1.
AY099694 mRNA. Translation: AAM20545.1.
AY128873 mRNA. Translation: AAM91273.1.
RefSeqiNP_001032120.1. NM_001037043.1. [Q9FKG3-2]
NP_200966.2. NM_125551.3. [Q9FKG3-1]
UniGeneiAt.29096.

Genome annotation databases

EnsemblPlantsiAT5G61580.1; AT5G61580.1; AT5G61580. [Q9FKG3-1]
GeneIDi836279.
KEGGiath:AT5G61580.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012239 Genomic DNA. Translation: BAB09002.1.
CP002688 Genomic DNA. Translation: AED97492.1.
CP002688 Genomic DNA. Translation: AED97493.1.
AY099694 mRNA. Translation: AAM20545.1.
AY128873 mRNA. Translation: AAM91273.1.
RefSeqiNP_001032120.1. NM_001037043.1. [Q9FKG3-2]
NP_200966.2. NM_125551.3. [Q9FKG3-1]
UniGeneiAt.29096.

3D structure databases

ProteinModelPortaliQ9FKG3.
SMRiQ9FKG3. Positions 87-494.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21523. 1 interaction.

Proteomic databases

PaxDbiQ9FKG3.
PRIDEiQ9FKG3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G61580.1; AT5G61580.1; AT5G61580. [Q9FKG3-1]
GeneIDi836279.
KEGGiath:AT5G61580.

Organism-specific databases

TAIRiAT5G61580.

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000016186.
InParanoidiQ9FKG3.
KOiK00850.
OMAiIVVQKGS.
PhylomeDBiQ9FKG3.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciARA:AT5G61580-MONOMER.
ARA:GQT-2054-MONOMER.
BRENDAi2.7.1.11. 399.
ReactomeiREACT_293115. Glycolysis.

Gene expression databases

GenevestigatoriQ9FKG3.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X.
InterProiIPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
    Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
    DNA Res. 5:203-216(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Characterisation of the ATP-dependent phosphofructokinase gene family from Arabidopsis thaliana."
    Mustroph A., Sonnewald U., Biemelt S.
    FEBS Lett. 581:2401-2410(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiPFKA4_ARATH
AccessioniPrimary (citable) accession number: Q9FKG3
Secondary accession number(s): Q2V2W5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 1, 2001
Last modified: April 1, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.