Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9FKG3

- PFKA4_ARATH

UniProt

Q9FKG3 - PFKA4_ARATH

Protein

ATP-dependent 6-phosphofructokinase 4, chloroplastic

Gene

PFK4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.1 PublicationUniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by AMP.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei152 – 1521ATP; via amide nitrogenUniRule annotation
    Sitei242 – 2421Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
    Active sitei271 – 2711Proton acceptorUniRule annotation
    Binding sitei370 – 3701SubstrateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi215 – 2162ATPUniRule annotation
    Nucleotide bindingi240 – 2434ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: TAIR
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. carbohydrate phosphorylation Source: GOC
    2. fructose 6-phosphate metabolic process Source: InterPro
    3. glycolytic process Source: TAIR

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G61580-MONOMER.
    ARA:GQT-2054-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinase 4, chloroplasticUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFK 4UniRule annotation
    Short name:
    Phosphofructokinase 4UniRule annotation
    Alternative name(s):
    Phosphohexokinase 4UniRule annotation
    Gene namesi
    Name:PFK4UniRule annotation
    Ordered Locus Names:At5g61580
    ORF Names:K11J9.3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G61580.

    Subcellular locationi

    Plastidchloroplast 1 Publication

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro
    2. chloroplast Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5454ChloroplastSequence AnalysisAdd
    BLAST
    Chaini55 – 530476ATP-dependent 6-phosphofructokinase 4, chloroplasticPRO_0000330771Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei121 – 1211PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9FKG3.
    PRIDEiQ9FKG3.

    Expressioni

    Tissue specificityi

    Expressed in leaves, stems and flowers.1 Publication

    Gene expression databases

    GenevestigatoriQ9FKG3.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    BioGridi21523. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FKG3.
    SMRiQ9FKG3. Positions 87-494.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni269 – 2713Substrate bindingUniRule annotation
    Regioni314 – 3163Substrate bindingUniRule annotation
    Regioni427 – 4304Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0205.
    HOGENOMiHOG000016186.
    InParanoidiQ9FKG3.
    KOiK00850.
    OMAiWLTQQIK.
    PhylomeDBiQ9FKG3.

    Family and domain databases

    HAMAPiMF_01981. Phosphofructokinase_II_X.
    InterProiIPR022953. Phosphofructokinase.
    IPR000023. Phosphofructokinase_dom.
    IPR012004. PyroP-dep_PFruKinase_TP0108.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9FKG3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEASISFLGS TKPNISLFNP SSNVLPRRDF PLPALKLKKV SVLPRILHQK    50
    RLIRAQCSDG FKPEEDDGFV LEDVPHLTKF LPDLPSYPNP LKESQAYAIV 100
    KRTFVSSEDV VAQNIVVQKG SKRGVHFRRA GPRERVYFRS DEVKACIVTC 150
    GGLCPGINTV IREIVCGLNN MYGVNNILGI QGGYRGFYSK NTMNLTPKVV 200
    NDIHKRGGTF LQTSRGGHDT AKIVDNIQDR GINQVYIIGG GGTQKGAEKI 250
    YEEVERRGLQ VAVSGIPKTI DNDIAVIDKS FGFDTAVEEA QRAINAAHVE 300
    VESVENGVGI VKLMGRYSGF IAMIATLANR DVDCCLIPES PFFLEGKGGL 350
    FEFIEERLKE NRHMVIVIAE GAGQDYVAQS MRASETKDAS GNRLLLDVGL 400
    WLTQQIKDHF TNVRKMMINM KYIDPTYMIR AIPSNASDNV YCTLLAQSAV 450
    HGAMAGYSGF TVGPVNSRHA YIPISQVTEV TNTVKLTDRM WARLLASTNQ 500
    PSFLTGEGAL QNVIDMETQE KIDNMKISSI 530
    Length:530
    Mass (Da):58,467
    Last modified:March 1, 2001 - v1
    Checksum:i7CF9AC8460F44DF4
    GO
    Isoform 2 (identifier: Q9FKG3-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         476-476: Missing.

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:529
    Mass (Da):58,339
    Checksum:i241B3E01D4D4D85D
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei476 – 4761Missing in isoform 2. CuratedVSP_033114

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB012239 Genomic DNA. Translation: BAB09002.1.
    CP002688 Genomic DNA. Translation: AED97492.1.
    CP002688 Genomic DNA. Translation: AED97493.1.
    AY099694 mRNA. Translation: AAM20545.1.
    AY128873 mRNA. Translation: AAM91273.1.
    RefSeqiNP_001032120.1. NM_001037043.1. [Q9FKG3-2]
    NP_200966.2. NM_125551.3. [Q9FKG3-1]
    UniGeneiAt.29096.

    Genome annotation databases

    EnsemblPlantsiAT5G61580.1; AT5G61580.1; AT5G61580. [Q9FKG3-1]
    GeneIDi836279.
    KEGGiath:AT5G61580.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB012239 Genomic DNA. Translation: BAB09002.1 .
    CP002688 Genomic DNA. Translation: AED97492.1 .
    CP002688 Genomic DNA. Translation: AED97493.1 .
    AY099694 mRNA. Translation: AAM20545.1 .
    AY128873 mRNA. Translation: AAM91273.1 .
    RefSeqi NP_001032120.1. NM_001037043.1. [Q9FKG3-2 ]
    NP_200966.2. NM_125551.3. [Q9FKG3-1 ]
    UniGenei At.29096.

    3D structure databases

    ProteinModelPortali Q9FKG3.
    SMRi Q9FKG3. Positions 87-494.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 21523. 1 interaction.

    Proteomic databases

    PaxDbi Q9FKG3.
    PRIDEi Q9FKG3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G61580.1 ; AT5G61580.1 ; AT5G61580 . [Q9FKG3-1 ]
    GeneIDi 836279.
    KEGGi ath:AT5G61580.

    Organism-specific databases

    TAIRi AT5G61580.

    Phylogenomic databases

    eggNOGi COG0205.
    HOGENOMi HOG000016186.
    InParanoidi Q9FKG3.
    KOi K00850.
    OMAi WLTQQIK.
    PhylomeDBi Q9FKG3.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci ARA:AT5G61580-MONOMER.
    ARA:GQT-2054-MONOMER.

    Gene expression databases

    Genevestigatori Q9FKG3.

    Family and domain databases

    HAMAPi MF_01981. Phosphofructokinase_II_X.
    InterProi IPR022953. Phosphofructokinase.
    IPR000023. Phosphofructokinase_dom.
    IPR012004. PyroP-dep_PFruKinase_TP0108.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000534. PPi_PFK_TP0108. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
      Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
      DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    4. "Characterisation of the ATP-dependent phosphofructokinase gene family from Arabidopsis thaliana."
      Mustroph A., Sonnewald U., Biemelt S.
      FEBS Lett. 581:2401-2410(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiPFKA4_ARATH
    AccessioniPrimary (citable) accession number: Q9FKG3
    Secondary accession number(s): Q2V2W5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3