Reviewed, UniProtKB/Swiss-Prot Q9FKF3 (PME63_ARATH)

Names and origin

Protein names

Recommended name:
    Putative pectinesterase 63
      Short name=PE 63
    EC=3.1.1.11
Alternative name(s):
    Pectin methylesterase 63
      Short name=AtPME63

Gene names

Name:	PME63
Synonyms:	ARATH63
Ordered Locus Names:	At5g61680
ORF Names:	K11J9.6

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Acts in the modification of cell walls via demethylesterification of cell wall pectin By similarity.
Catalytic activity	Pectin + n H2O = n methanol + pectate.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
Subcellular location	Secreted › cell wall By similarity.
Sequence similarities	Belongs to the pectinesterase family.
Sequence caution	The sequence BAB09012.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Aspartyl esterase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall modification Inferred from electronic annotation. Source: InterPro
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aspartyl esterase activity Inferred from electronic annotation. Source: UniProtKB-KW pectinesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 338	314	Putative pectinesterase 63		PRO_0000371708
Sites
	Active site	174	1	Proton donor By similarity
	Active site	195	1	Nucleophile By similarity
	Binding site	116	1	Substrate By similarity
	Binding site	151	1	Substrate By similarity
	Binding site	252	1	Substrate By similarity
	Site	173	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9FKF3-1 [UniParc]. Last modified May 5, 2009. Version 2. Checksum: 430DBDBBF559526A	FASTA	338	37,324
	10 20 30 40 50 60 MGYNYVSLIV TILLVVITSP VVFGNDAAPI PENKGRIEQW FNTNVKQNGR GHFKTITEAI 70 80 90 100 110 120 NSVRAGNTRR VIIKIGPGVY KEKVTIDRSK PFITLYGHPN AMPVLTFDGT AAQYGTVDSA 130 140 150 160 170 180 TLIVLSDYFM AVNIILKNSA PMPDGKRKGA QALSMRISGN KAAFYNCKFY GYQDTICDDT 190 200 210 220 230 240 GNHFFKDCYI EGTFDFIFGS GRSLYLGTQL NVVGDGIRVI TAHAGKSAAE KSGYSFVHCK 250 260 270 280 290 300 VTGTGTGIYL GRSWMSHPKV VYAYTDMSSV VNPSGWQENR EAGRDKTVFY GEYKCTGTGS 310 320 330 HKEKRVKYTQ DIDDIEAKYF ISLGYIQGSS WLLPPPSF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones." Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S. DNA Res. 5:203-216(1998) [PubMed: 9734815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Pectin methylesterases: sequence-structural features and phylogenetic relationships." Markovic O., Janecek S. Carbohydr. Res. 339:2281-2295(2004) [PubMed: 15337457] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB012239 Genomic DNA. Translation: BAB09012.1. Sequence problems.
IPI	IPI00539362.
RefSeq	NP_200976.1.
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q9FKF3.
Genome annotation databases
GeneID	836290.
GenomeReviews	Gene locus AT5G61680 in contig BA000015_GR.
Organism-specific databases
TAIR	At5g61680.
Gene expression databases
Genevestigator	Q9FKF3.
Family and domain databases
InterPro	IPR012334. Pectin_lyas_fold. IPR018040. Pectinesterase_AS. IPR000070. Pectinesterase_cat. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF01095. Pectinesterase. 1 hit. [Graphical view]
PROSITE	PS00800. PECTINESTERASE_1. False negative. PS00503. PECTINESTERASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PME63_ARATH

Accession

Primary (citable) accession number: Q9FKF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	May 5, 2009
Last modified:	October 13, 2009
This is version 32 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)