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Protein

Pantoate--beta-alanine ligase

Gene

At5g48840

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the condensation of pantoate with beta-alanine to form pantothenate. Essential for panthotenate biosynthesis.2 Publications

Catalytic activityi

ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate.1 Publication

Enzyme regulationi

Enzyme kinetics do not match Michaelis-Menten kinetics, suggesting allosteric behavior. Inhibited by high pantoate levels.1 Publication

pH dependencei

Optimum pH is 9.0.1 Publication

Pathwayi: (R)-pantothenate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes (R)-pantothenate from (R)-pantoate and beta-alanine.
Proteins known to be involved in this subpathway in this organism are:
  1. Pantoate--beta-alanine ligase (At5g48840)
This subpathway is part of the pathway (R)-pantothenate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-pantothenate from (R)-pantoate and beta-alanine, the pathway (R)-pantothenate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei39Proton donorBy similarity1
Binding sitei63Beta-alanineBy similarity1
Binding sitei63PantoateBy similarity1
Binding sitei181PantoateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi32 – 39ATPBy similarity8
Nucleotide bindingi175 – 178ATPBy similarity4
Nucleotide bindingi212 – 215ATPBy similarity4

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • pantoate-beta-alanine ligase activity Source: TAIR
  • protein homodimerization activity Source: TAIR

GO - Biological processi

  • embryo development ending in seed dormancy Source: TAIR
  • pantothenate biosynthetic process Source: TAIR
  • pantothenate biosynthetic process from valine Source: GO_Central

Keywordsi

Molecular functionAllosteric enzyme, Ligase
Biological processPantothenate biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G48840-MONOMER
MetaCyc:AT5G48840-MONOMER
BRENDAi6.3.2.1 399
UniPathwayiUPA00028; UER00005

Names & Taxonomyi

Protein namesi
Recommended name:
Pantoate--beta-alanine ligase (EC:6.3.2.1)
Alternative name(s):
Pantoate-activating enzyme
Pantothenate synthetase
Gene namesi
Ordered Locus Names:At5g48840
ORF Names:K24G6.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G48840
TAIRilocus:2156564 AT5G48840

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality due to arrest of embryogenesis at the preglobular stage when homozygous.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi132E → A: Reduces allosteric properties. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001282991 – 310Pantoate--beta-alanine ligaseAdd BLAST310

Proteomic databases

PaxDbiQ9FKB3
PRIDEiQ9FKB3

Expressioni

Tissue specificityi

Expressed in roots, cotyledons, leaves, stems, cauline leaves, stigma, sepals and petals.1 Publication

Gene expression databases

ExpressionAtlasiQ9FKB3 baseline and differential
GenevisibleiQ9FKB3 AT

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: TAIR

Protein-protein interaction databases

STRINGi3702.AT5G48840.1

Structurei

3D structure databases

ProteinModelPortaliQ9FKB3
SMRiQ9FKB3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pantothenate synthetase family.Curated

Phylogenomic databases

eggNOGiKOG3042 Eukaryota
COG0414 LUCA
HOGENOMiHOG000175516
InParanoidiQ9FKB3
KOiK01918
OMAiLFNMVQP
OrthoDBiEOG09360G9W
PhylomeDBiQ9FKB3

Family and domain databases

CDDicd00560 PanC, 1 hit
Gene3Di3.40.50.620, 1 hit
HAMAPiMF_00158 PanC, 1 hit
InterProiView protein in InterPro
IPR003721 Pantoate_ligase
IPR014729 Rossmann-like_a/b/a_fold
PANTHERiPTHR21299:SF1 PTHR21299:SF1, 1 hit
PfamiView protein in Pfam
PF02569 Pantoate_ligase, 1 hit
TIGRFAMsiTIGR00018 panC, 1 hit

Sequencei

Sequence statusi: Complete.

Q9FKB3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPEVIRDKD SMRKWSRAMR SQGKTIGLVP TMGYLHEGHL SLVRQSLALT
60 70 80 90 100
DVTVVSIYVN PGQFSPTEDL STYPSDFSGD LTKLAALSGG KVVVFNPKNL
110 120 130 140 150
YDYGGETKKI NDGGGNGGRV VSCVEEGGLG HETWIRVERL EKGFCGKSRP
160 170 180 190 200
VFFRGVATIV TKLFNIVEPD VALFGKKDYQ QWRIIQRMVR DLNFGIEIVG
210 220 230 240 250
SDIAREKDGL AMSSRNVRLS DEERQRALSI SRSLAMAKAS VAEGKTNCAE
260 270 280 290 300
LKDMIIQQVV GSAGRVDYVE IVDQETLEGV EEIKSGVVIC VAAWFGTVRL
310
IDNIEINVSL
Length:310
Mass (Da):34,137
Last modified:March 1, 2001 - v1
Checksum:iAD07D927625AD1A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012242 Genomic DNA Translation: BAB09437.1
CP002688 Genomic DNA Translation: AED95732.1
AK175238 mRNA Translation: BAD43001.1
RefSeqiNP_199695.1, NM_124261.4
UniGeneiAt.29821

Genome annotation databases

EnsemblPlantsiAT5G48840.1; AT5G48840.1; AT5G48840
GeneIDi834942
GrameneiAT5G48840.1; AT5G48840.1; AT5G48840
KEGGiath:AT5G48840

Similar proteinsi

Entry informationi

Entry nameiPANC_ARATH
AccessioniPrimary (citable) accession number: Q9FKB3
Secondary accession number(s): Q682X9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2002
Last sequence update: March 1, 2001
Last modified: April 25, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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