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Q9FKB3

- PANC_ARATH

UniProt

Q9FKB3 - PANC_ARATH

Protein

Pantoate--beta-alanine ligase

Gene

At5g48840

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the condensation of pantoate with beta-alanine to form pantothenate. Essential for panthotenate biosynthesis.2 Publications

    Catalytic activityi

    ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate.1 Publication

    Enzyme regulationi

    Enzyme kinetics do not match Michaelis-Menten kinetics, suggesting allosteric behavior. Inhibited by high pantoate levels.1 Publication

    pH dependencei

    Optimum pH is 9.0.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei39 – 391Proton donorBy similarity
    Binding sitei63 – 631Beta-alanineBy similarity
    Binding sitei63 – 631PantoateBy similarity
    Binding sitei181 – 1811PantoateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi32 – 398ATPBy similarity
    Nucleotide bindingi175 – 1784ATPBy similarity
    Nucleotide bindingi212 – 2154ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. pantoate-beta-alanine ligase activity Source: TAIR
    3. protein homodimerization activity Source: TAIR

    GO - Biological processi

    1. embryo development ending in seed dormancy Source: TAIR
    2. pantothenate biosynthetic process Source: TAIR

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Pantothenate biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G48840-MONOMER.
    MetaCyc:AT5G48840-MONOMER.
    UniPathwayiUPA00028; UER00005.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pantoate--beta-alanine ligase (EC:6.3.2.1)
    Alternative name(s):
    Pantoate-activating enzyme
    Pantothenate synthetase
    Gene namesi
    Ordered Locus Names:At5g48840
    ORF Names:K24G6.18
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G48840.

    Subcellular locationi

    Cytoplasmcytosol 1 Publication

    GO - Cellular componenti

    1. cytosol Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Embryonic lethality due to arrest of embryogenesis at the preglobular stage when homozygous.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi132 – 1321E → A: Reduces allosteric properties. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 310310Pantoate--beta-alanine ligasePRO_0000128299Add
    BLAST

    Proteomic databases

    PaxDbiQ9FKB3.
    PRIDEiQ9FKB3.

    Expressioni

    Tissue specificityi

    Expressed in roots, cotyledons, leaves, stems, cauline leaves, stigma, sepals and petals.1 Publication

    Gene expression databases

    GenevestigatoriQ9FKB3.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FKB3.
    SMRiQ9FKB3. Positions 5-303.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pantothenate synthetase family.Curated

    Phylogenomic databases

    eggNOGiCOG0414.
    HOGENOMiHOG000175516.
    InParanoidiQ9FKB3.
    KOiK01918.
    OMAiECPIVRE.
    PhylomeDBiQ9FKB3.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    HAMAPiMF_00158. PanC.
    InterProiIPR003721. Pantoate_ligase.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR21299:SF1. PTHR21299:SF1. 1 hit.
    PfamiPF02569. Pantoate_ligase. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00018. panC. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9FKB3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEPEVIRDKD SMRKWSRAMR SQGKTIGLVP TMGYLHEGHL SLVRQSLALT    50
    DVTVVSIYVN PGQFSPTEDL STYPSDFSGD LTKLAALSGG KVVVFNPKNL 100
    YDYGGETKKI NDGGGNGGRV VSCVEEGGLG HETWIRVERL EKGFCGKSRP 150
    VFFRGVATIV TKLFNIVEPD VALFGKKDYQ QWRIIQRMVR DLNFGIEIVG 200
    SDIAREKDGL AMSSRNVRLS DEERQRALSI SRSLAMAKAS VAEGKTNCAE 250
    LKDMIIQQVV GSAGRVDYVE IVDQETLEGV EEIKSGVVIC VAAWFGTVRL 300
    IDNIEINVSL 310
    Length:310
    Mass (Da):34,137
    Last modified:March 1, 2001 - v1
    Checksum:iAD07D927625AD1A6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB012242 Genomic DNA. Translation: BAB09437.1.
    CP002688 Genomic DNA. Translation: AED95732.1.
    AK175238 mRNA. Translation: BAD43001.1.
    RefSeqiNP_199695.1. NM_124261.3.
    UniGeneiAt.29821.

    Genome annotation databases

    EnsemblPlantsiAT5G48840.1; AT5G48840.1; AT5G48840.
    GeneIDi834942.
    KEGGiath:AT5G48840.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB012242 Genomic DNA. Translation: BAB09437.1 .
    CP002688 Genomic DNA. Translation: AED95732.1 .
    AK175238 mRNA. Translation: BAD43001.1 .
    RefSeqi NP_199695.1. NM_124261.3.
    UniGenei At.29821.

    3D structure databases

    ProteinModelPortali Q9FKB3.
    SMRi Q9FKB3. Positions 5-303.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q9FKB3.
    PRIDEi Q9FKB3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G48840.1 ; AT5G48840.1 ; AT5G48840 .
    GeneIDi 834942.
    KEGGi ath:AT5G48840.

    Organism-specific databases

    TAIRi AT5G48840.

    Phylogenomic databases

    eggNOGi COG0414.
    HOGENOMi HOG000175516.
    InParanoidi Q9FKB3.
    KOi K01918.
    OMAi ECPIVRE.
    PhylomeDBi Q9FKB3.

    Enzyme and pathway databases

    UniPathwayi UPA00028 ; UER00005 .
    BioCyci ARA:AT5G48840-MONOMER.
    MetaCyc:AT5G48840-MONOMER.

    Miscellaneous databases

    PROi Q9FKB3.

    Gene expression databases

    Genevestigatori Q9FKB3.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    HAMAPi MF_00158. PanC.
    InterProi IPR003721. Pantoate_ligase.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR21299:SF1. PTHR21299:SF1. 1 hit.
    Pfami PF02569. Pantoate_ligase. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00018. panC. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
      Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
      DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. Cited for: SUBCELLULAR LOCATION.
    5. "Molecular adaptation and allostery in plant pantothenate synthetases."
      Jonczyk R., Genschel U.
      J. Biol. Chem. 281:37435-37446(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, ALLOSTERIC PROPERTIES, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, MUTAGENESIS OF GLU-132.
    6. "Pantothenate synthetase is essential but not limiting for pantothenate biosynthesis in Arabidopsis."
      Jonczyk R., Ronconi S., Rychlik M., Genschel U.
      Plant Mol. Biol. 66:1-14(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiPANC_ARATH
    AccessioniPrimary (citable) accession number: Q9FKB3
    Secondary accession number(s): Q682X9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2002
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3