Q9FK60 (SODC3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Superoxide dismutase [Cu-Zn] 3
EC=1.15.1.1

Alternative name(s):
Copper/zinc superoxide dismutase 3

Gene names

Name:	CSD3
Ordered Locus Names:	At5g18100
ORF Names:	MRG7.6

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	164 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Destroys radicals which are normally produced within the cells and which are toxic to biological systems By similarity.
Catalytic activity	2 superoxide + 2 H+ = O2 + H2O2.
Cofactor	Binds 1 copper ion per subunit By similarity. Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Peroxisome Ref.5 Ref.6.
Tissue specificity	Expressed in leaves (at protein level). Ref.5
Induction	Upon photosynthetically active radiation (PAR) (e.g. light fluence) increase or after high-light pulse, and UV-B treatment. Accumulates in response to ozone fumigation, during recovery. Repressed by salt stress. Ref.5 Ref.8
Sequence similarities	Belongs to the Cu-Zn superoxide dismutase family.

Ontologies

Keywords
Cellular component	Peroxisome
Coding sequence diversity	Alternative splicing
Ligand	Copper Metal-binding Zinc
Molecular function	Antioxidant Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to UV-B Inferred from expression pattern Ref.5. Source: UniProtKB cellular response to high light intensity Inferred from expression pattern Ref.5. Source: UniProtKB cellular response to ozone Inferred from expression pattern Ref.5. Source: UniProtKB cellular response to salt stress Inferred from expression pattern Ref.8. Source: UniProtKB removal of superoxide radicals Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	extracellular region Inferred from Biological aspect of Ancestor. Source: RefGenome peroxisome Inferred from direct assay Ref.6. Source: TAIR vacuole Inferred from direct assay Ref.7. Source: TAIR
Molecular_function	copper ion binding Inferred from Biological aspect of Ancestor. Source: RefGenome superoxide dismutase activity Inferred from Biological aspect of Ancestor. Source: RefGenome zinc ion binding Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9FK60-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9FK60-2)

The sequence of this isoform differs from the canonical sequence as follows:
     135-137: GHK → TKH
     138-164: Missing.

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 164	164	Superoxide dismutase [Cu-Zn] 3		PRO_0000419143
Regions
	Motif	162 – 164	3	Peroxisome localization signal By similarity
Sites
	Metal binding	51	1	Copper; catalytic By similarity
	Metal binding	53	1	Copper; catalytic By similarity
	Metal binding	68	1	Copper; catalytic By similarity
	Metal binding	68	1	Zinc; structural By similarity
	Metal binding	76	1	Zinc; structural By similarity
	Metal binding	85	1	Zinc; structural By similarity
	Metal binding	88	1	Zinc; structural By similarity
	Metal binding	125	1	Copper; catalytic By similarity
Amino acid modifications
	Disulfide bond	62 ↔ 151		By similarity
Natural variations
	Alternative sequence	135 – 137	3	GHK → TKH in isoform 2.		VSP_044111
	Alternative sequence	138 – 164	27	Missing in isoform 2.		VSP_044112

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: A80B9F4A79D0F365	FASTA	164	16,941
	10 20 30 40 50 60 MEAPRGNLRA VALIAGDNNV RGCLQFVQDI SGTTHVTGKI SGLSPGFHGF HIHSFGDTTN 70 80 90 100 110 120 GCISTGPHFN PLNRVHGPPN EEERHAGDLG NILAGSNGVA EILIKDKHIP LSGQYSILGR 130 140 150 160 AVVVHADPDD LGKGGHKLSK STGNAGSRVG CGIIGLQSSA DAKL
	Isoform 2 [UniParc]. Checksum: BBD55B91BCC424BD	FASTA	137	14,412
	10 20 30 40 50 60 MEAPRGNLRA VALIAGDNNV RGCLQFVQDI SGTTHVTGKI SGLSPGFHGF HIHSFGDTTN 70 80 90 100 110 120 GCISTGPHFN PLNRVHGPPN EEERHAGDLG NILAGSNGVA EILIKDKHIP LSGQYSILGR 130 AVVVHADPDD LGKGTKH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones." Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S. DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[5]	"Superoxide dismutase in Arabidopsis: an eclectic enzyme family with disparate regulation and protein localization." Kliebenstein D.J., Monde R.A., Last R.L. Plant Physiol. 118:637-650(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-164 (ISOFORM 1), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION BY LIGHT; UV-B AND OZONE, GENE FAMILY. Strain: cv. Columbia.
[6]	"Proteome analysis of Arabidopsis leaf peroxisomes reveals novel targeting peptides, metabolic pathways, and defense mechanisms." Reumann S., Babujee L., Ma C., Wienkoop S., Siemsen T., Antonicelli G.E., Rasche N., Lueder F., Weckwerth W., Jahn O. Plant Cell 19:3170-3193(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 85-105 AND 108-120, SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]	"The vegetative vacuole proteome of Arabidopsis thaliana reveals predicted and unexpected proteins." Carter C., Pan S., Zouhar J., Avila E.L., Girke T., Raikhel N.V. Plant Cell 16:3285-3303(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]	"Long-term effects of mild salt stress on growth, ion accumulation and superoxide dismutase expression of Arabidopsis rosette leaves." Attia H., Arnaud N., Karray N., Lachaal M. Physiol. Plantarum 132:293-305(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY SALT. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF061520 mRNA. Translation: AAC24833.1. AB012246 Genomic DNA. Translation: BAB09468.1. CP002688 Genomic DNA. Translation: AED92506.1. CP002688 Genomic DNA. Translation: AED92507.1. AK117742 mRNA. Translation: BAC42391.1. BT003689 mRNA. Translation: AAO39917.1.
IPI	IPI00521381. IPI00891861.
PIR	T51731.
RefSeq	NP_001119245.1. NM_001125773.1. NP_197311.1. NM_121815.3.
UniGene	At.49035. At.7547.
3D structure databases
HSSP	HSSP built from PDB template 1P1V based on UniProtKB P00441.
ProteinModelPortal	Q9FK60.
SMR	Q9FK60. Positions 9-157.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9FK60. 1 interaction.
STRING	3702.AT5G18100.1-P.
Proteomic databases
PRIDE	Q9FK60.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G18100.1; AT5G18100.1; AT5G18100.
GeneID	831928.
KEGG	ath:AT5G18100.
Organism-specific databases
TAIR	At5g18100.
Phylogenomic databases
eggNOG	COG2032.
HOGENOM	HOG000263447.
InParanoid	Q9FK60.
KO	K04565.
OMA	DKERHAG.
PhylomeDB	Q9FK60.
ProtClustDB	PLN02642.
Gene expression databases
ArrayExpress	Q9FK60.
Genevestigator	Q9FK60.
Family and domain databases
Gene3D	2.60.40.200. 1 hit.
InterPro	IPR024134. SOD_Cu/Zn_/chaperones. IPR018152. SOD_Cu/Zn_BS. IPR001424. SOD_Cu_Zn_dom. [Graphical view]
PANTHER	PTHR10003. PTHR10003. 1 hit.
Pfam	PF00080. Sod_Cu. 1 hit. [Graphical view]
PRINTS	PR00068. CUZNDISMTASE.
SUPFAM	SSF49329. SOD_Cu_Zn. 1 hit.
PROSITE	PS00332. SOD_CU_ZN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SODC3_ARATH

Accession

Primary (citable) accession number: Q9FK60
Secondary accession number(s): B3H6P9, O81236

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2012
Last sequence update:	March 1, 2001
Last modified:	April 3, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program