Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9FK29 (UBC33_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable ubiquitin-conjugating enzyme E2 33

EC=6.3.2.19
Alternative name(s):
Ubiquitin carrier protein 33
Gene names
Name:UBC33
Ordered Locus Names:At5g50430
ORF Names:MXI22.15
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length243 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins By similarity.

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Subcellular location

Membrane; Single-pass membrane protein Potential.

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ubiquitin-protein ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9FK29-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9FK29-2)

The sequence of this isoform differs from the canonical sequence as follows:
     38-51: HYVLEGSEGTPFAG → R
Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 243243Probable ubiquitin-conjugating enzyme E2 33
PRO_0000345198

Regions

Transmembrane220 – 24021Helical; Potential

Sites

Active site871Glycyl thioester intermediate By similarity

Natural variations

Alternative sequence38 – 5114HYVLE…TPFAG → R in isoform 2.
VSP_034928

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 0A461F79073C954D

FASTA24327,357
        10         20         30         40         50         60 
MAEKACIKRL QKEYRALCKE PVSHVVARPS PNDILEWHYV LEGSEGTPFA GGFYYGKIKF 

        70         80         90        100        110        120 
PPEYPYKPPG ITMTTPNGRF VTQKKICLSM SDFHPESWNP MWSVSSILTG LLSFMMDNSP 

       130        140        150        160        170        180 
TTGSVNTSVA EKQRLAKSSL AFNCKSVTFR KLFPEYVEKY SQQQVAEEEA ATQQTTTSEN 

       190        200        210        220        230        240 
QDFPQKDNAK VESEKSVGLK KESIQEVGLK ERRRNKKEAL PGWIVLLLVS IVGVVMALPL 


LQL 

« Hide

Isoform 2 [UniParc].

Checksum: 594B6B3795013B6A
Show »

FASTA23026,067

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[4]"Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-219, GENE FAMILY, NOMENCLATURE.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB012248 Genomic DNA. Translation: BAB09462.1.
CP002688 Genomic DNA. Translation: AED95943.1.
CP002688 Genomic DNA. Translation: AED95944.1.
CP002688 Genomic DNA. Translation: AED95945.1.
AF380654 mRNA. Translation: AAK55735.1.
AY142040 mRNA. Translation: AAM98304.1.
DQ027046 mRNA. Translation: AAY44872.1.
RefSeqNP_001032048.2. NM_001036971.2.
NP_001032049.1. NM_001036972.2.
NP_199854.1. NM_124425.3.
UniGeneAt.6859.

3D structure databases

ProteinModelPortalQ9FK29.
SMRQ9FK29. Positions 8-207.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ9FK29.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G50430.1; AT5G50430.1; AT5G50430. [Q9FK29-1]
AT5G50430.2; AT5G50430.2; AT5G50430. [Q9FK29-1]
GeneID835111.
KEGGath:AT5G50430.

Organism-specific databases

TAIRAT5G50430.

Phylogenomic databases

eggNOGCOG5078.
HOGENOMHOG000170329.
InParanoidQ9FK29.
KOK04554.
OMATPNGRFV.
PhylomeDBQ9FK29.
ProtClustDBCLSN2681888.

Enzyme and pathway databases

BioCycARA:AT5G50430-MONOMER.
ARA:GQT-1704-MONOMER.
ARA:GQT-1705-MONOMER.
UniPathwayUPA00143.

Gene expression databases

GenevestigatorQ9FK29.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ9FK29.

Entry information

Entry nameUBC33_ARATH
AccessionPrimary (citable) accession number: Q9FK29
Secondary accession number(s): Q2V2Z8, Q4TYX8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names