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Protein

Probable ubiquitin-conjugating enzyme E2 33

Gene

UBC33

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.By similarity

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathway: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei87 – 871Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G50430-MONOMER.
ARA:GQT-1704-MONOMER.
ARA:GQT-1705-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ubiquitin-conjugating enzyme E2 33 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein 33
Gene namesi
Name:UBC33
Ordered Locus Names:At5g50430
ORF Names:MXI22.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G50430.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei220 – 24021HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 243243Probable ubiquitin-conjugating enzyme E2 33PRO_0000345198Add
BLAST

Proteomic databases

PRIDEiQ9FK29.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G50430.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FK29.
SMRiQ9FK29. Positions 8-207.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000170329.
InParanoidiQ9FK29.
KOiK04554.
OMAiCESIEVI.
PhylomeDBiQ9FK29.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9FK29-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEKACIKRL QKEYRALCKE PVSHVVARPS PNDILEWHYV LEGSEGTPFA
60 70 80 90 100
GGFYYGKIKF PPEYPYKPPG ITMTTPNGRF VTQKKICLSM SDFHPESWNP
110 120 130 140 150
MWSVSSILTG LLSFMMDNSP TTGSVNTSVA EKQRLAKSSL AFNCKSVTFR
160 170 180 190 200
KLFPEYVEKY SQQQVAEEEA ATQQTTTSEN QDFPQKDNAK VESEKSVGLK
210 220 230 240
KESIQEVGLK ERRRNKKEAL PGWIVLLLVS IVGVVMALPL LQL
Length:243
Mass (Da):27,357
Last modified:March 1, 2001 - v1
Checksum:i0A461F79073C954D
GO
Isoform 2 (identifier: Q9FK29-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     38-51: HYVLEGSEGTPFAG → R

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:230
Mass (Da):26,067
Checksum:i594B6B3795013B6A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei38 – 5114HYVLE…TPFAG → R in isoform 2. CuratedVSP_034928Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012248 Genomic DNA. Translation: BAB09462.1.
CP002688 Genomic DNA. Translation: AED95943.1.
CP002688 Genomic DNA. Translation: AED95944.1.
CP002688 Genomic DNA. Translation: AED95945.1.
AF380654 mRNA. Translation: AAK55735.1.
AY142040 mRNA. Translation: AAM98304.1.
DQ027046 mRNA. Translation: AAY44872.1.
RefSeqiNP_001032048.2. NM_001036971.2. [Q9FK29-1]
NP_001032049.1. NM_001036972.2. [Q9FK29-2]
NP_199854.1. NM_124425.3. [Q9FK29-1]
UniGeneiAt.6859.

Genome annotation databases

EnsemblPlantsiAT5G50430.1; AT5G50430.1; AT5G50430. [Q9FK29-1]
AT5G50430.2; AT5G50430.2; AT5G50430. [Q9FK29-1]
GeneIDi835111.
KEGGiath:AT5G50430.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012248 Genomic DNA. Translation: BAB09462.1.
CP002688 Genomic DNA. Translation: AED95943.1.
CP002688 Genomic DNA. Translation: AED95944.1.
CP002688 Genomic DNA. Translation: AED95945.1.
AF380654 mRNA. Translation: AAK55735.1.
AY142040 mRNA. Translation: AAM98304.1.
DQ027046 mRNA. Translation: AAY44872.1.
RefSeqiNP_001032048.2. NM_001036971.2. [Q9FK29-1]
NP_001032049.1. NM_001036972.2. [Q9FK29-2]
NP_199854.1. NM_124425.3. [Q9FK29-1]
UniGeneiAt.6859.

3D structure databases

ProteinModelPortaliQ9FK29.
SMRiQ9FK29. Positions 8-207.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G50430.1.

Proteomic databases

PRIDEiQ9FK29.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G50430.1; AT5G50430.1; AT5G50430. [Q9FK29-1]
AT5G50430.2; AT5G50430.2; AT5G50430. [Q9FK29-1]
GeneIDi835111.
KEGGiath:AT5G50430.

Organism-specific databases

TAIRiAT5G50430.

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000170329.
InParanoidiQ9FK29.
KOiK04554.
OMAiCESIEVI.
PhylomeDBiQ9FK29.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciARA:AT5G50430-MONOMER.
ARA:GQT-1704-MONOMER.
ARA:GQT-1705-MONOMER.

Miscellaneous databases

PROiQ9FK29.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
    Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
    DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
    Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
    Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-219, GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiUBC33_ARATH
AccessioniPrimary (citable) accession number: Q9FK29
Secondary accession number(s): Q2V2Z8, Q4TYX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: March 1, 2001
Last modified: June 24, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.