Q9FK29 (UBC33_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable ubiquitin-conjugating enzyme E2 33
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein 33

Gene names

Name:	UBC33
Ordered Locus Names:	At5g50430
ORF Names:	MXI22.15

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9FK29-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9FK29-2)

The sequence of this isoform differs from the canonical sequence as follows:
     38-51: HYVLEGSEGTPFAG → R

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 243	243	Probable ubiquitin-conjugating enzyme E2 33		PRO_0000345198
Regions
	Transmembrane	220 – 240	21	Helical; Potential
Sites
	Active site	87	1	Glycyl thioester intermediate By similarity
Natural variations
	Alternative sequence	38 – 51	14	HYVLE…TPFAG → R in isoform 2.		VSP_034928

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 0A461F79073C954D	FASTA	243	27,357
	10 20 30 40 50 60 MAEKACIKRL QKEYRALCKE PVSHVVARPS PNDILEWHYV LEGSEGTPFA GGFYYGKIKF 70 80 90 100 110 120 PPEYPYKPPG ITMTTPNGRF VTQKKICLSM SDFHPESWNP MWSVSSILTG LLSFMMDNSP 130 140 150 160 170 180 TTGSVNTSVA EKQRLAKSSL AFNCKSVTFR KLFPEYVEKY SQQQVAEEEA ATQQTTTSEN 190 200 210 220 230 240 QDFPQKDNAK VESEKSVGLK KESIQEVGLK ERRRNKKEAL PGWIVLLLVS IVGVVMALPL LQL
	Isoform 2 [UniParc]. Checksum: 594B6B3795013B6A	FASTA	230	26,067
	10 20 30 40 50 60 MAEKACIKRL QKEYRALCKE PVSHVVARPS PNDILEWRGF YYGKIKFPPE YPYKPPGITM 70 80 90 100 110 120 TTPNGRFVTQ KKICLSMSDF HPESWNPMWS VSSILTGLLS FMMDNSPTTG SVNTSVAEKQ 130 140 150 160 170 180 RLAKSSLAFN CKSVTFRKLF PEYVEKYSQQ QVAEEEAATQ QTTTSENQDF PQKDNAKVES 190 200 210 220 230 EKSVGLKKES IQEVGLKERR RNKKEALPGW IVLLLVSIVG VVMALPLLQL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones." Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S. DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[4]	"Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis." Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J. Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-219, GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB012248 Genomic DNA. Translation: BAB09462.1. CP002688 Genomic DNA. Translation: AED95943.1. CP002688 Genomic DNA. Translation: AED95944.1. CP002688 Genomic DNA. Translation: AED95945.1. AF380654 mRNA. Translation: AAK55735.1. AY142040 mRNA. Translation: AAM98304.1. DQ027046 mRNA. Translation: AAY44872.1.
IPI	IPI00542401. IPI00656807.
RefSeq	NP_001032048.2. NM_001036971.2. NP_001032049.1. NM_001036972.2. NP_199854.1. NM_124425.3.
UniGene	At.6859.
3D structure databases
ProteinModelPortal	Q9FK29.
SMR	Q9FK29. Positions 8-207.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G50430.1; AT5G50430.1; AT5G50430. AT5G50430.2; AT5G50430.2; AT5G50430.
GeneID	835111.
KEGG	ath:AT5G50430.
Organism-specific databases
TAIR	At5g50430.
Phylogenomic databases
eggNOG	COG5078.
HOGENOM	HOG000170329.
InParanoid	Q9FK29.
KO	K04554.
OMA	SFMTGDE.
PhylomeDB	Q9FK29.
ProtClustDB	CLSN2681888.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Genevestigator	Q9FK29.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. False negative. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBC33_ARATH

Accession

Primary (citable) accession number: Q9FK29
Secondary accession number(s): Q2V2Z8, Q4TYX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	March 1, 2001
Last modified:	May 29, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program