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Q9FK29

- UBC33_ARATH

UniProt

Q9FK29 - UBC33_ARATH

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Protein

Probable ubiquitin-conjugating enzyme E2 33

Gene

UBC33

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.By similarity

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei87 – 871Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. protein ubiquitination Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G50430-MONOMER.
ARA:GQT-1704-MONOMER.
ARA:GQT-1705-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ubiquitin-conjugating enzyme E2 33 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein 33
Gene namesi
Name:UBC33
Ordered Locus Names:At5g50430
ORF Names:MXI22.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G50430.

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 243243Probable ubiquitin-conjugating enzyme E2 33PRO_0000345198Add
BLAST

Proteomic databases

PRIDEiQ9FK29.

Expressioni

Gene expression databases

GenevestigatoriQ9FK29.

Structurei

3D structure databases

ProteinModelPortaliQ9FK29.
SMRiQ9FK29. Positions 8-207.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei220 – 24021HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000170329.
InParanoidiQ9FK29.
KOiK04554.
OMAiFMTGDES.
PhylomeDBiQ9FK29.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9FK29-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEKACIKRL QKEYRALCKE PVSHVVARPS PNDILEWHYV LEGSEGTPFA
60 70 80 90 100
GGFYYGKIKF PPEYPYKPPG ITMTTPNGRF VTQKKICLSM SDFHPESWNP
110 120 130 140 150
MWSVSSILTG LLSFMMDNSP TTGSVNTSVA EKQRLAKSSL AFNCKSVTFR
160 170 180 190 200
KLFPEYVEKY SQQQVAEEEA ATQQTTTSEN QDFPQKDNAK VESEKSVGLK
210 220 230 240
KESIQEVGLK ERRRNKKEAL PGWIVLLLVS IVGVVMALPL LQL
Length:243
Mass (Da):27,357
Last modified:March 1, 2001 - v1
Checksum:i0A461F79073C954D
GO
Isoform 2 (identifier: Q9FK29-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     38-51: HYVLEGSEGTPFAG → R

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:230
Mass (Da):26,067
Checksum:i594B6B3795013B6A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei38 – 5114HYVLE…TPFAG → R in isoform 2. CuratedVSP_034928Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB012248 Genomic DNA. Translation: BAB09462.1.
CP002688 Genomic DNA. Translation: AED95943.1.
CP002688 Genomic DNA. Translation: AED95944.1.
CP002688 Genomic DNA. Translation: AED95945.1.
AF380654 mRNA. Translation: AAK55735.1.
AY142040 mRNA. Translation: AAM98304.1.
DQ027046 mRNA. Translation: AAY44872.1.
RefSeqiNP_001032048.2. NM_001036971.2. [Q9FK29-1]
NP_001032049.1. NM_001036972.2. [Q9FK29-2]
NP_199854.1. NM_124425.3. [Q9FK29-1]
UniGeneiAt.6859.

Genome annotation databases

EnsemblPlantsiAT5G50430.1; AT5G50430.1; AT5G50430. [Q9FK29-1]
AT5G50430.2; AT5G50430.2; AT5G50430. [Q9FK29-1]
GeneIDi835111.
KEGGiath:AT5G50430.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB012248 Genomic DNA. Translation: BAB09462.1 .
CP002688 Genomic DNA. Translation: AED95943.1 .
CP002688 Genomic DNA. Translation: AED95944.1 .
CP002688 Genomic DNA. Translation: AED95945.1 .
AF380654 mRNA. Translation: AAK55735.1 .
AY142040 mRNA. Translation: AAM98304.1 .
DQ027046 mRNA. Translation: AAY44872.1 .
RefSeqi NP_001032048.2. NM_001036971.2. [Q9FK29-1 ]
NP_001032049.1. NM_001036972.2. [Q9FK29-2 ]
NP_199854.1. NM_124425.3. [Q9FK29-1 ]
UniGenei At.6859.

3D structure databases

ProteinModelPortali Q9FK29.
SMRi Q9FK29. Positions 8-207.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q9FK29.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G50430.1 ; AT5G50430.1 ; AT5G50430 . [Q9FK29-1 ]
AT5G50430.2 ; AT5G50430.2 ; AT5G50430 . [Q9FK29-1 ]
GeneIDi 835111.
KEGGi ath:AT5G50430.

Organism-specific databases

TAIRi AT5G50430.

Phylogenomic databases

eggNOGi COG5078.
HOGENOMi HOG000170329.
InParanoidi Q9FK29.
KOi K04554.
OMAi FMTGDES.
PhylomeDBi Q9FK29.

Enzyme and pathway databases

UniPathwayi UPA00143 .
BioCyci ARA:AT5G50430-MONOMER.
ARA:GQT-1704-MONOMER.
ARA:GQT-1705-MONOMER.

Miscellaneous databases

PROi Q9FK29.

Gene expression databases

Genevestigatori Q9FK29.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
    Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
    DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
    Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
    Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-219, GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiUBC33_ARATH
AccessioniPrimary (citable) accession number: Q9FK29
Secondary accession number(s): Q2V2Z8, Q4TYX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: March 1, 2001
Last modified: October 1, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3