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Q9FK29

- UBC33_ARATH

UniProt

Q9FK29 - UBC33_ARATH

Protein

Probable ubiquitin-conjugating enzyme E2 33

Gene

UBC33

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.By similarity

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei87 – 871Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein ubiquitination Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G50430-MONOMER.
    ARA:GQT-1704-MONOMER.
    ARA:GQT-1705-MONOMER.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable ubiquitin-conjugating enzyme E2 33 (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein 33
    Gene namesi
    Name:UBC33
    Ordered Locus Names:At5g50430
    ORF Names:MXI22.15
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G50430.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 243243Probable ubiquitin-conjugating enzyme E2 33PRO_0000345198Add
    BLAST

    Proteomic databases

    PRIDEiQ9FK29.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9FK29.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FK29.
    SMRiQ9FK29. Positions 8-207.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei220 – 24021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5078.
    HOGENOMiHOG000170329.
    InParanoidiQ9FK29.
    KOiK04554.
    OMAiFMTGDES.
    PhylomeDBiQ9FK29.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9FK29-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAEKACIKRL QKEYRALCKE PVSHVVARPS PNDILEWHYV LEGSEGTPFA    50
    GGFYYGKIKF PPEYPYKPPG ITMTTPNGRF VTQKKICLSM SDFHPESWNP 100
    MWSVSSILTG LLSFMMDNSP TTGSVNTSVA EKQRLAKSSL AFNCKSVTFR 150
    KLFPEYVEKY SQQQVAEEEA ATQQTTTSEN QDFPQKDNAK VESEKSVGLK 200
    KESIQEVGLK ERRRNKKEAL PGWIVLLLVS IVGVVMALPL LQL 243
    Length:243
    Mass (Da):27,357
    Last modified:March 1, 2001 - v1
    Checksum:i0A461F79073C954D
    GO
    Isoform 2 (identifier: Q9FK29-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         38-51: HYVLEGSEGTPFAG → R

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:230
    Mass (Da):26,067
    Checksum:i594B6B3795013B6A
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei38 – 5114HYVLE…TPFAG → R in isoform 2. CuratedVSP_034928Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB012248 Genomic DNA. Translation: BAB09462.1.
    CP002688 Genomic DNA. Translation: AED95943.1.
    CP002688 Genomic DNA. Translation: AED95944.1.
    CP002688 Genomic DNA. Translation: AED95945.1.
    AF380654 mRNA. Translation: AAK55735.1.
    AY142040 mRNA. Translation: AAM98304.1.
    DQ027046 mRNA. Translation: AAY44872.1.
    RefSeqiNP_001032048.2. NM_001036971.2. [Q9FK29-1]
    NP_001032049.1. NM_001036972.2. [Q9FK29-2]
    NP_199854.1. NM_124425.3. [Q9FK29-1]
    UniGeneiAt.6859.

    Genome annotation databases

    EnsemblPlantsiAT5G50430.1; AT5G50430.1; AT5G50430. [Q9FK29-1]
    AT5G50430.2; AT5G50430.2; AT5G50430. [Q9FK29-1]
    GeneIDi835111.
    KEGGiath:AT5G50430.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    PlantsUBQ

    A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB012248 Genomic DNA. Translation: BAB09462.1 .
    CP002688 Genomic DNA. Translation: AED95943.1 .
    CP002688 Genomic DNA. Translation: AED95944.1 .
    CP002688 Genomic DNA. Translation: AED95945.1 .
    AF380654 mRNA. Translation: AAK55735.1 .
    AY142040 mRNA. Translation: AAM98304.1 .
    DQ027046 mRNA. Translation: AAY44872.1 .
    RefSeqi NP_001032048.2. NM_001036971.2. [Q9FK29-1 ]
    NP_001032049.1. NM_001036972.2. [Q9FK29-2 ]
    NP_199854.1. NM_124425.3. [Q9FK29-1 ]
    UniGenei At.6859.

    3D structure databases

    ProteinModelPortali Q9FK29.
    SMRi Q9FK29. Positions 8-207.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q9FK29.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G50430.1 ; AT5G50430.1 ; AT5G50430 . [Q9FK29-1 ]
    AT5G50430.2 ; AT5G50430.2 ; AT5G50430 . [Q9FK29-1 ]
    GeneIDi 835111.
    KEGGi ath:AT5G50430.

    Organism-specific databases

    TAIRi AT5G50430.

    Phylogenomic databases

    eggNOGi COG5078.
    HOGENOMi HOG000170329.
    InParanoidi Q9FK29.
    KOi K04554.
    OMAi FMTGDES.
    PhylomeDBi Q9FK29.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci ARA:AT5G50430-MONOMER.
    ARA:GQT-1704-MONOMER.
    ARA:GQT-1705-MONOMER.

    Miscellaneous databases

    PROi Q9FK29.

    Gene expression databases

    Genevestigatori Q9FK29.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
      Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
      DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    4. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
      Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
      Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-219, GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiUBC33_ARATH
    AccessioniPrimary (citable) accession number: Q9FK29
    Secondary accession number(s): Q2V2Z8, Q4TYX8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 22, 2008
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3