Q9FK28 (MEM12_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Membrin-12
Short name=AtMEMB12

Alternative name(s):
Golgi SNAP receptor complex member 2-2

Gene names

Name:	MEMB12
Ordered Locus Names:	At5g50440
ORF Names:	MXI22.16

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in transport of proteins from the cis/medial-Golgi to the trans-Golgi network By similarity.
Subcellular location	Golgi apparatus membrane; Single-pass type IV membrane protein Potential.
Sequence similarities	Belongs to the GOSR2 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Golgi apparatus Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of innate immune response Inferred from mutant phenotype PubMed 21549312. Source: TAIR regulation of protein secretion Inferred from mutant phenotype PubMed 21549312. Source: TAIR
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell Golgi stack Inferred from electronic annotation. Source: InterPro cis-Golgi network Inferred from direct assay PubMed 22253225. Source: TAIR integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 219	219	Membrin-12		PRO_0000212555
Regions
	Topological domain	1 – 197	197	Cytoplasmic Potential
	Transmembrane	198 – 215	18	Helical; Anchor for type IV membrane protein; Potential
	Topological domain	216 – 219	4	Vesicular Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9FK28 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 9885F002AF89B4EC	FASTA	219	25,105
	10 20 30 40 50 60 MASGTVGGLS EVYSSAKRIL LRARNGIEKL ERFDSDPTDL ASSVKRDITE VQSLCSNMDG 70 80 90 100 110 120 LWRSIPVKSQ RDLWRRKSEQ VGEEAEYLNQ SLEKYMWRNQ RKMLEAKERA DLLGRGSGEG 130 140 150 160 170 180 AHILQIFDEE AQGMNSVKNS KRMLEDSFQS GVAILSKYAE QRDRLKSAQR KALDVLNTVG 190 200 210 LSNSVLRLIE RRNRVDTWIK YAGMIATLVI LYLFIRWTR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones." Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S. DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB012248 Genomic DNA. Translation: BAB09463.1. CP002688 Genomic DNA. Translation: AED95946.1. BT004999 mRNA. Translation: AAO50532.1.
IPI	IPI00517700.
RefSeq	NP_199855.1. NM_124426.4.
UniGene	At.29730.
3D structure databases
ProteinModelPortal	Q9FK28.
SMR	Q9FK28. Positions 132-187.
ModBase	Search...
Proteomic databases
PaxDb	Q9FK28.
PRIDE	Q9FK28.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G50440.1; AT5G50440.1; AT5G50440.
GeneID	835112.
KEGG	ath:AT5G50440.
Organism-specific databases
GeneFarm	4298. 433.
TAIR	At5g50440.
Phylogenomic databases
eggNOG	NOG148687.
HOGENOM	HOG000264938.
InParanoid	Q9FK28.
KO	K08496.
OMA	EVYSSAK.
PhylomeDB	Q9FK28.
ProtClustDB	CLSN2683951.
Gene expression databases
ArrayExpress	Q9FK28.
Genevestigator	Q9FK28.
GermOnline	AT5G50440. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR027027. GOSR2/Membrin/Bos1. [Graphical view]
PIRSF	PIRSF028865. Membrin-2. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MEM12_ARATH

Accession

Primary (citable) accession number: Q9FK28

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 17, 2003
Last sequence update:	March 1, 2001
Last modified:	May 29, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program