Reviewed,
UniProtKB/Swiss-Prot Q9FK05 (PME61_ARATH)
Last modified
February 9, 2010.
Version 50.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Probable pectinesterase/pectinesterase inhibitor 61 Including the following 2 domains: 1- Recommended name: Pectinesterase inhibitor 61 Alternative name(s): Pectin methylesterase inhibitor 61 2- Recommended name: Pectinesterase 61 Short name=PE 61 EC=3.1.1.11 Alternative name(s): Pectin methylesterase 61 Short name=AtPME61 AtPMEpcrF | ||||||||
| Gene names |
| ||||||||
| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 587 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Acts in the modification of cell walls via demethylesterification of cell wall pectin By similarity. |
| Catalytic activity | Pectin + n H2O = n methanol + pectate. |
| Pathway | Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5. |
| Subcellular location | Membrane; Single-pass membrane protein Potential. |
| Tissue specificity | Expressed in siliques, floral stems and rosettes leaves. Ref.3 Ref.5 |
| Developmental stage | Expressed throughout silique development. Ref.5 |
| Miscellaneous | The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport. |
| Sequence similarities | In the N-terminal section; belongs to the PMEI family. In the C-terminal section; belongs to the pectinesterase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Cell wall biogenesis/degradation |
| Cellular component | Membrane |
| Domain | Transmembrane |
| Molecular function | Aspartyl esterase Hydrolase |
| PTM | Disulfide bond Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | cell wall modification Inferred from electronic annotation. Source: InterPro |
| Cellular component | cell wall Inferred from electronic annotation. Source: InterPro integral to membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | aspartyl esterase activity Inferred from electronic annotation. Source: UniProtKB-KW enzyme inhibitor activityInferred from electronic annotation. Source: InterPro pectinesterase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 587 | 587 | Probable pectinesterase/pectinesterase inhibitor 61 | PRO_0000371707 | |||||||
Regions | |||||||||||
| Transmembrane | 35 – 55 | 21 | Potential | ||||||||
| Region | 69 – 223 | 155 | Pectinesterase inhibitor 61 | ||||||||
| Region | 273 – 571 | 299 | Pectinesterase 61 | ||||||||
Sites | |||||||||||
| Active site | 402 | 1 | Proton donor; for pectinesterase activity By similarity | ||||||||
| Active site | 423 | 1 | Nucleophile; for pectinesterase activity By similarity | ||||||||
| Binding site | 349 | 1 | Substrate; for pectinesterase activity By similarity | ||||||||
| Binding site | 379 | 1 | Substrate; for pectinesterase activity By similarity | ||||||||
| Binding site | 491 | 1 | Substrate; for pectinesterase activity By similarity | ||||||||
| Binding site | 493 | 1 | Substrate; for pectinesterase activity By similarity | ||||||||
| Site | 401 | 1 | Transition state stabilizer By similarity | ||||||||
Amino acid modifications | |||||||||||
| Modified residue | 539 | 1 | Phosphoserine Ref.6 | ||||||||
| Disulfide bond | 416 ↔ 436 | By similarity | |||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones." Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S. DNA Res. 5:203-216(1998) [PubMed: 9734815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [2] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [3] | "Characterization of the pectin methylesterase-like gene AtPME3: a new member of a gene family comprising at least 12 genes in Arabidopsis thaliana." Micheli F., Holliger C., Goldberg R., Richard L. Gene 220:13-20(1998) [PubMed: 9767082] [Abstract] Cited for: TISSUE SPECIFICITY. |
| [4] | "Pectin methylesterases: sequence-structural features and phylogenetic relationships." Markovic O., Janecek S. Carbohydr. Res. 339:2281-2295(2004) [PubMed: 15337457] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE. |
| [5] | "Comprehensive expression profiling of the pectin methylesterase gene family during silique development in Arabidopsis thaliana." Louvet R., Cavel E., Gutierrez L., Guenin S., Roger D., Gillet F., Guerineau F., Pelloux J. Planta 224:782-791(2006) [PubMed: 16622707] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. |
| [6] | "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks." Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S. Plant Physiol. 150:889-903(2009) [PubMed: 19376835] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-539, MASS SPECTROMETRY. Tissue: Seedling. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AB013388 Genomic DNA. Translation: BAB09799.1. AF360340 mRNA. Translation: AAK28637.1. AY051077 mRNA. Translation: AAK93754.1. |
| IPI | IPI00530003. |
| RefSeq | NP_200149.1. |
| UniGene | At.24561 At.29558 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1GQ8 based on UniProtKB P83218. |
| SMR | Q9FK05. Positions 73-224, 267-585. |
| ModBase | Search... |
Proteomic databases | |
| PRIDE | Q9FK05. |
Genome annotation databases | |
| GeneID | 835418. |
| GenomeReviews | Gene locus AT5G53370 in contig BA000015_GR. |
| KEGG | ath:AT5G53370. |
| NMPDR | fig|3702.1.peg.27231. |
Organism-specific databases | |
| GeneFarm | 298. 8. |
| TAIR | At5g53370. |
Phylogenomic databases | |
| eggNOG | COG4677. |
| HOGENOM | HBG747179. |
| InParanoid | Q9FK05. |
| OMA | ISIHACK. |
Gene expression databases | |
| ArrayExpress | Q9FK05. |
| Genevestigator | Q9FK05. |
Family and domain databases | |
| InterPro | IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. IPR018040. Pectinesterase_AS. IPR000070. Pectinesterase_cat. IPR006501. Pectinesterase_inhib. [Graphical view] |
| Gene3D | G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit. G3DSA:1.20.140.40. Pectinesterase_inhib. 1 hit. |
| Pfam | PF01095. Pectinesterase. 1 hit. PF04043. PMEI. 1 hit. [Graphical view] |
| SMART | SM00856. PMEI. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01614. PME_inhib. 1 hit. |
| PROSITE | PS00800. PECTINESTERASE_1. False negative. PS00503. PECTINESTERASE_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | PME61_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q9FK05 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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