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Protein

Mannan endo-1,4-beta-mannosidase 7

Gene

MAN7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Required for both, loosening of the micropylar endosperm, and rupture of the seed coat in germinating seeds. May participate in the hydrolysis of the mannans in the cell wall of germinating seeds.1 Publication

Catalytic activityi

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei203Proton donorBy similarity1
Active sitei320NucleophileBy similarity1

GO - Molecular functioni

  • mannan endo-1,4-beta-mannosidase activity Source: TAIR

GO - Biological processi

  • mannan catabolic process Source: GO_Central
  • seed germination Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT5G66460-MONOMER.
BRENDAi3.2.1.78. 399.

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan endo-1,4-beta-mannosidase 7 (EC:3.2.1.78)
Alternative name(s):
Beta-mannanase 7
Endo-beta-1,4-mannanase 7
Short name:
AtMAN7
Gene namesi
Name:MAN7
Ordered Locus Names:At5g66460
ORF Names:K1F13.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G66460.

Subcellular locationi

GO - Cellular componenti

  • cell periphery Source: TAIR
  • extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000027748026 – 431Mannan endo-1,4-beta-mannosidase 7Add BLAST406

Proteomic databases

PaxDbiQ9FJZ3.

Expressioni

Tissue specificityi

Expressed in stems, flowers, siliques and seeds (PubMed:16897088). Expressed in root vasculature, leaf hydathodes, anther filaments, stigma, sepal vasculature, at the base and apical parts of siliques, and replum. Expressed in the micropylar endosperm and radicle tip in early germinating seeds (PubMed:23461773).2 Publications

Inductioni

By gibberellin in germinating seeds.1 Publication

Gene expression databases

GenevisibleiQ9FJZ3. AT.

Interactioni

Protein-protein interaction databases

BioGridi22020. 1 interactor.
IntActiQ9FJZ3. 2 interactors.
STRINGi3702.AT5G66460.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FJZ3.
SMRiQ9FJZ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IIVS. Eukaryota.
COG3934. LUCA.
HOGENOMiHOG000169952.
InParanoidiQ9FJZ3.
KOiK19355.
OMAiWIRTHIS.
OrthoDBiEOG09360BHK.
PhylomeDBiQ9FJZ3.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FJZ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLALFPFL AIVIQLSCWE LGTDALPSGG FVRTKGVQFS LNGYPYYANG
60 70 80 90 100
FNAYWLMYVA SDPSQRSKIS TAFQDASRHG LTVARTWAFS DGGYRALQYS
110 120 130 140 150
PGSYNEDMFQ GLDFALAEAR RHGIKIILSF ANNYESFGGR KQYVDWARSR
160 170 180 190 200
GRPVSSEDDF FTDSLVKDFY KNHIKAVLNR FNTFTKVHYK DDPTIMAWEL
210 220 230 240 250
MNEPRCPSDP SGRAIQAWIT EMAAHVKSLD RNHLLEAGLE GFYGQSSPQS
260 270 280 290 300
KTLNPPGQFG TDFIANNRIP GIDFVTVHSY PDEWFPDSSE QSQMDFLNKW
310 320 330 340 350
LDAHIQDAQN VLHKPIILAE FGKSMKKPGY TPAQRDIVFN TVYSKIYGSA
360 370 380 390 400
KRGGAAAGGL FWQLLVNGID NFQDGYGIIL SQSSSTVNVI SQQSRKLTLI
410 420 430
RKIFARMINV EKWKRARGQG QVGKRGHKIN N
Length:431
Mass (Da):48,573
Last modified:March 1, 2001 - v1
Checksum:iFA5BB16113E0F3DF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB013389 Genomic DNA. Translation: BAB10922.1.
CP002688 Genomic DNA. Translation: AED98217.1.
BT000452 mRNA. Translation: AAN17429.1.
BT008749 mRNA. Translation: AAP49511.1.
RefSeqiNP_201447.1. NM_126044.4.
UniGeneiAt.28865.
At.67287.

Genome annotation databases

EnsemblPlantsiAT5G66460.1; AT5G66460.1; AT5G66460.
GeneIDi836778.
GrameneiAT5G66460.1; AT5G66460.1; AT5G66460.
KEGGiath:AT5G66460.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB013389 Genomic DNA. Translation: BAB10922.1.
CP002688 Genomic DNA. Translation: AED98217.1.
BT000452 mRNA. Translation: AAN17429.1.
BT008749 mRNA. Translation: AAP49511.1.
RefSeqiNP_201447.1. NM_126044.4.
UniGeneiAt.28865.
At.67287.

3D structure databases

ProteinModelPortaliQ9FJZ3.
SMRiQ9FJZ3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22020. 1 interactor.
IntActiQ9FJZ3. 2 interactors.
STRINGi3702.AT5G66460.1.

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Proteomic databases

PaxDbiQ9FJZ3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G66460.1; AT5G66460.1; AT5G66460.
GeneIDi836778.
GrameneiAT5G66460.1; AT5G66460.1; AT5G66460.
KEGGiath:AT5G66460.

Organism-specific databases

TAIRiAT5G66460.

Phylogenomic databases

eggNOGiENOG410IIVS. Eukaryota.
COG3934. LUCA.
HOGENOMiHOG000169952.
InParanoidiQ9FJZ3.
KOiK19355.
OMAiWIRTHIS.
OrthoDBiEOG09360BHK.
PhylomeDBiQ9FJZ3.

Enzyme and pathway databases

BioCyciARA:AT5G66460-MONOMER.
BRENDAi3.2.1.78. 399.

Miscellaneous databases

PROiQ9FJZ3.

Gene expression databases

GenevisibleiQ9FJZ3. AT.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMAN7_ARATH
AccessioniPrimary (citable) accession number: Q9FJZ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.