Q9FJZ3 (MAN7_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannan endo-1,4-beta-mannosidase 7
EC=3.2.1.78

Alternative name(s):
Beta-mannanase 7
Endo-beta-1,4-mannanase 7
Short name=AtMAN7

Gene names

Name:	MAN7
Ordered Locus Names:	At5g66460
ORF Names:	K1F13.12

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Subcellular location	Secreted Potential.
Tissue specificity	Expressed in stems, flowers, siliques and seeds. Ref.4
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro mannan endo-1,4-beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 431	406	Mannan endo-1,4-beta-mannosidase 7		PRO_0000277480
Sites
	Active site	203	1	Proton donor By similarity
	Active site	320	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9FJZ3 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: FA5BB16113E0F3DF	FASTA	431	48,573
	10 20 30 40 50 60 MKLLALFPFL AIVIQLSCWE LGTDALPSGG FVRTKGVQFS LNGYPYYANG FNAYWLMYVA 70 80 90 100 110 120 SDPSQRSKIS TAFQDASRHG LTVARTWAFS DGGYRALQYS PGSYNEDMFQ GLDFALAEAR 130 140 150 160 170 180 RHGIKIILSF ANNYESFGGR KQYVDWARSR GRPVSSEDDF FTDSLVKDFY KNHIKAVLNR 190 200 210 220 230 240 FNTFTKVHYK DDPTIMAWEL MNEPRCPSDP SGRAIQAWIT EMAAHVKSLD RNHLLEAGLE 250 260 270 280 290 300 GFYGQSSPQS KTLNPPGQFG TDFIANNRIP GIDFVTVHSY PDEWFPDSSE QSQMDFLNKW 310 320 330 340 350 360 LDAHIQDAQN VLHKPIILAE FGKSMKKPGY TPAQRDIVFN TVYSKIYGSA KRGGAAAGGL 370 380 390 400 410 420 FWQLLVNGID NFQDGYGIIL SQSSSTVNVI SQQSRKLTLI RKIFARMINV EKWKRARGQG 430 QVGKRGHKIN N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones." Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S. DNA Res. 5:203-216(1998) [PubMed: 9734815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar." Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F. Funct. Integr. Genomics 7:1-16(2007) [PubMed: 16897088] [Abstract] Cited for: GENE FAMILY, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB013389 Genomic DNA. Translation: BAB10922.1. CP002688 Genomic DNA. Translation: AED98217.1. BT000452 mRNA. Translation: AAN17429.1. BT008749 mRNA. Translation: AAP49511.1.
IPI	IPI00536325.
RefSeq	NP_201447.1. NM_126044.3.
UniGene	At.28865. At.67287.
3D structure databases
ProteinModelPortal	Q9FJZ3.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9FJZ3. 2 interactions.
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
Proteomic databases
PRIDE	Q9FJZ3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G66460.1; AT5G66460.1; AT5G66460.
GeneID	836778.
GenomeReviews	Gene locus AT5G66460 in contig BA000015_GR.
KEGG	ath:AT5G66460.
NMPDR	fig|3702.1.peg.28750.
Organism-specific databases
TAIR	At5g66460.
Phylogenomic databases
eggNOG	euNOG11227.
GeneTree	EPGT00050000010376.
HOGENOM	HBG316339.
InParanoid	Q9FJZ3.
OMA	NRIPGID.
PhylomeDB	Q9FJZ3.
ProtClustDB	CLSN2916497.
Gene expression databases
ArrayExpress	Q9FJZ3.
Genevestigator	Q9FJZ3.
Family and domain databases
InterPro	IPR001547. Glyco_hydro_5. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAN7_ARATH

Accession

Primary (citable) accession number: Q9FJZ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	March 1, 2001
Last modified:	December 14, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program