Reviewed, UniProtKB/Swiss-Prot Q9FJV0 (M2K6_ARATH)

Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase kinase 6
      Short name=MAP kinase kinase 6
      Short name=AtMKK6
    EC=2.7.12.2
Alternative name(s):
    Protein Arabidopsis NQK1 homolog

Gene names

Name:	MKK6
Synonyms:	ANQ1
Ordered Locus Names:	At5g56580
ORF Names:	MIK19.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the regulation of plant cytokinesis during meiosis and mitosis. Activates MPK13 in vitro. Ref.1 Ref.4
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Activated through serine and threonine phosphorylation By similarity.
Tissue specificity	Expressed in roots, stems and flower buds. Ref.4
Post-translational modification	Phosphorylated on Ser-221 and Thr-227, which activates the enzyme By similarity.
Disruption phenotype	Plants exhibit a dwarf phenotype with fewer flowers, large cells with multiple nuclei in various organs and large malformed pollen grains. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase activity Inferred from direct assay. Source: TAIR protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q2V412	1	EBI-1238868,EBI-1238781

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Mitogen-activated protein kinase kinase 6		PRO_0000245825
Regions
	Domain	70 – 331	262	Protein kinase
	Nucleotide binding	76 – 84	9	ATP By similarity
Sites
	Active site	193	1	Proton acceptor By similarity
	Binding site	99	1	ATP By similarity
Amino acid modifications
	Modified residue	221	1	Phosphoserine By similarity
	Modified residue	227	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9FJV0-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: C95016C1DAE69CED	FASTA	356	39,837
	10 20 30 40 50 60 MVKIKSNLKQ LKLSVPAQES PISSFLTASG TFHDGDFLLN QKGLRLTSDE KQSRQSDSKE 70 80 90 100 110 120 LDFEITAEDL ETVKVIGKGS GGVVQLVRHK WVGKFFAMKV IQMNIQEEIR KQIVQELKIN 130 140 150 160 170 180 QASSQCPHVV VCYHSFYHNG AFSLVLEYMD RGSLADVIRQ VKTILEPYLA VVCKQVLLGL 190 200 210 220 230 240 VYLHNERHVI HRDIKPSNLL VNHKGEVKIS DFGVSASLAS SMGQRDTFVG TYNYMSPERI 250 260 270 280 290 300 SGSTYDYSSD IWSLGMSVLE CAIGRFPYLE SEDQQNPPSF YELLAAIVEN PPPTAPSDQF 310 320 330 340 350 SPEFCSFVSA CIQKDPPARA SSLDLLSHPF IKKFEDKDID LGILVGTLEP PVNYLR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"NQK1/NtMEK1 is a MAPKK that acts in the NPK1 MAPKKK-mediated MAPK cascade and is required for plant cytokinesis." Soyano T., Nishihama R., Morikiyo K., Ishikawa M., Machida Y. Genes Dev. 17:1055-1067(2003) [PubMed: 12704083] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones." Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S. DNA Res. 5:203-216(1998) [PubMed: 9734815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Mitogen-activated protein kinase cascades in plants: a new nomenclature." MAPK group Trends Plant Sci. 7:301-308(2002) [PubMed: 12119167] [Abstract] Cited for: NOMENCLATURE.
[4]	"The Arabidopsis thaliana MEK AtMKK6 activates the MAP kinase AtMPK13." Melikant B., Giuliani C., Halbmayer-Watzina S., Limmongkon A., Heberle-Bors E., Wilson C. FEBS Lett. 576:5-8(2004) [PubMed: 15474000] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB104460 mRNA. Translation: BAC76067.1. AB013392 Genomic DNA. Translation: BAB09875.1.
IPI	IPI00537754.
RefSeq	NP_200469.1.
UniGene	At.29375
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9FJV0. 5 interactions.
STRING	Q9FJV0.
Proteomic databases
PRIDE	Q9FJV0.
Genome annotation databases
GeneID	835759.
GenomeReviews	Gene locus AT5G56580 in contig BA000015_GR.
KEGG	ath:AT5G56580.
NMPDR	fig|3702.1.peg.27598.
Organism-specific databases
GeneFarm	876. 89.
TAIR	At5g56580.
Phylogenomic databases
OMA	FEPICEL.
Enzyme and pathway databases
BRENDA	2.7.12.2. 302.
Gene expression databases
Genevestigator	Q9FJV0.
GermOnline	AT5G56580. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

M2K6_ARATH

Accession

Primary (citable) accession number: Q9FJV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)