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Protein

Glucan endo-1,3-beta-glucosidase 13

Gene

At5g56590

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei264 – 2641NucleophileBy similarity
Active sitei328 – 3281NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation, Plant defense

Enzyme and pathway databases

BioCyciARA:AT5G56590-MONOMER.

Protein family/group databases

CAZyiCBM43. Carbohydrate-Binding Module Family 43.
GH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase 13 (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase 13
Short name:
(1->3)-beta-glucanase 13
Beta-1,3-endoglucanase 13
Short name:
Beta-1,3-glucanase 13
Gene namesi
Ordered Locus Names:At5g56590
ORF Names:MIK19.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G56590.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: TAIR
  • anchored component of plasma membrane Source: TAIR
  • extracellular region Source: UniProtKB-KW
  • plant-type cell wall Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 471449Glucan endo-1,3-beta-glucosidase 13PRO_0000251273Add
BLAST
Propeptidei472 – 50635Removed in mature formSequence AnalysisPRO_0000251274Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi70 – 701N-linked (GlcNAc...)Sequence Analysis
Glycosylationi127 – 1271N-linked (GlcNAc...)Sequence Analysis
Glycosylationi175 – 1751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi212 – 2121N-linked (GlcNAc...)Sequence Analysis
Glycosylationi356 – 3561N-linked (GlcNAc...)Sequence Analysis
Glycosylationi361 – 3611N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi370 ↔ 433By similarity
Glycosylationi459 – 4591N-linked (GlcNAc...)Sequence Analysis
Glycosylationi465 – 4651N-linked (GlcNAc...)Sequence Analysis
Lipidationi471 – 4711GPI-anchor amidated serineSequence Analysis

Post-translational modificationi

Contains two additional disulfide bonds.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ9FJU9.
PRIDEiQ9FJU9.

Structurei

3D structure databases

ProteinModelPortaliQ9FJU9.
SMRiQ9FJU9. Positions 26-346.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG259646.
HOGENOMiHOG000238220.
InParanoidiQ9FJU9.
OMAiIMVTETG.
PhylomeDBiQ9FJU9.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
PF07983. X8. 1 hit.
[Graphical view]
SMARTiSM00768. X8. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FJU9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARDFKLIFS ISILLLLLDC CYGGKVGVCY GRSADDLPTP SKVVQLIQQH
60 70 80 90 100
NIKYVRIYDY NSQVLKAFGN TSIELMIGVP NSDLNAFSQS QSNVDTWLKN
110 120 130 140 150
SVLPYYPTTK ITYITVGAES TDDPHINASS FVVPAMQNVL TALRKVGLSR
160 170 180 190 200
RIKVSTTLSL GILSRSFPPS AGAFNSSYAY FLRPMLEFLA ENKSPFMIDL
210 220 230 240 250
YPYYAYRDSP NNVSLDYVLF ESSSEVIDPN TGLLYKNMFD AQVDALYYAL
260 270 280 290 300
TALNFRTIKI MVTETGWPTK GSPKEKAAAS SDNAETYNSN IIRHVVTNQG
310 320 330 340 350
TPAKPGEAMN VYIFSLFNEN RKAGLDSERN WGLFYPDQTS VYQLDFTGKS
360 370 380 390 400
NGFHSNSSGT NSSGSSNSWC IASSKASERD LKGALDWACG PGNVDCTAIQ
410 420 430 440 450
PSQPCFQPDT LVSHASFVFN SYFQQNRATD VACSFGGAGV KVNKDPSYDK
460 470 480 490 500
CIYITAGGNK TKATNATALT SSASTPRGNE LLQWILKLCL MISLFFSLQT

MNSQAL
Length:506
Mass (Da):55,604
Last modified:March 1, 2001 - v1
Checksum:iDC314FEF619BE810
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB013392 Genomic DNA. Translation: BAB09876.1.
CP002688 Genomic DNA. Translation: AED96784.1.
AY133637 mRNA. Translation: AAM91467.1.
AY075591 mRNA. Translation: AAL91612.1.
RefSeqiNP_200470.1. NM_125042.3.
UniGeneiAt.43021.

Genome annotation databases

EnsemblPlantsiAT5G56590.1; AT5G56590.1; AT5G56590.
GeneIDi835760.
KEGGiath:AT5G56590.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB013392 Genomic DNA. Translation: BAB09876.1.
CP002688 Genomic DNA. Translation: AED96784.1.
AY133637 mRNA. Translation: AAM91467.1.
AY075591 mRNA. Translation: AAL91612.1.
RefSeqiNP_200470.1. NM_125042.3.
UniGeneiAt.43021.

3D structure databases

ProteinModelPortaliQ9FJU9.
SMRiQ9FJU9. Positions 26-346.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM43. Carbohydrate-Binding Module Family 43.
GH17. Glycoside Hydrolase Family 17.

Proteomic databases

PaxDbiQ9FJU9.
PRIDEiQ9FJU9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G56590.1; AT5G56590.1; AT5G56590.
GeneIDi835760.
KEGGiath:AT5G56590.

Organism-specific databases

TAIRiAT5G56590.

Phylogenomic databases

eggNOGiNOG259646.
HOGENOMiHOG000238220.
InParanoidiQ9FJU9.
OMAiIMVTETG.
PhylomeDBiQ9FJU9.

Enzyme and pathway databases

BioCyciARA:AT5G56590-MONOMER.

Miscellaneous databases

PROiQ9FJU9.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
PF07983. X8. 1 hit.
[Graphical view]
SMARTiSM00768. X8. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
    Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
    DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins."
    Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C., Jensen O.N.
    Mol. Cell. Proteomics 2:1261-1270(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiE1313_ARATH
AccessioniPrimary (citable) accession number: Q9FJU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: March 1, 2001
Last modified: June 24, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.