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Q9FJU4

- MIOX5_ARATH

UniProt

Q9FJU4 - MIOX5_ARATH

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Protein
Inositol oxygenase 5
Gene
MIOX5, At5g56640, MIK19.9
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in the biosynthesis of UDP-glucuronic acid (UDP-GlcA), providing nucleotide sugars for cell-wall polymers. May be also involved in plant ascorbate biosynthesis.2 Publications

Catalytic activityi

Myo-inositol + O2 = D-glucuronate + H2O.

Cofactori

Binds 2 iron ions per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei54 – 541Substrate By similarity
Metal bindingi125 – 1251Iron 1 By similarity
Metal bindingi150 – 1501Iron 1 By similarity
Metal bindingi151 – 1511Iron 1 By similarity
Metal bindingi151 – 1511Iron 2 By similarity
Binding sitei154 – 1541Substrate By similarity
Metal bindingi223 – 2231Iron 2 By similarity
Metal bindingi249 – 2491Iron 2 By similarity
Metal bindingi282 – 2821Iron 1 By similarity

GO - Molecular functioni

  1. inositol oxygenase activity Source: UniProtKB-EC
  2. iron ion binding Source: InterPro

GO - Biological processi

  1. L-ascorbic acid biosynthetic process Source: UniProtKB-KW
  2. inositol catabolic process Source: InterPro
  3. syncytium formation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Ascorbate biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G56640-MONOMER.
UniPathwayiUPA00111; UER00527.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol oxygenase 5 (EC:1.13.99.1)
Alternative name(s):
Myo-inositol oxygenase 5
Short name:
AtMIOX5
Short name:
MI oxygenase 5
Gene namesi
Name:MIOX5
Ordered Locus Names:At5g56640
ORF Names:MIK19.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G56640.

Subcellular locationi

Cytoplasm Inferred

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 314314Inositol oxygenase 5
PRO_0000079157Add
BLAST

Proteomic databases

PaxDbiQ9FJU4.
PRIDEiQ9FJU4.

Expressioni

Tissue specificityi

Expressed in flowers and siliques.1 Publication

Gene expression databases

GenevestigatoriQ9FJU4.

Structurei

3D structure databases

ProteinModelPortaliQ9FJU4.
SMRiQ9FJU4. Positions 53-314.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni112 – 1143Substrate binding By similarity
Regioni171 – 1722Substrate binding By similarity
Regioni249 – 2502Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG135479.
HOGENOMiHOG000163182.
InParanoidiQ9FJU4.
KOiK00469.
OMAiNIWECCE.
PhylomeDBiQ9FJU4.

Family and domain databases

InterProiIPR007828. Inositol_oxygenase.
[Graphical view]
PANTHERiPTHR12588. PTHR12588. 1 hit.
PfamiPF05153. DUF706. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FJU4-1 [UniParc]FASTAAdd to Basket

« Hide

MNISVENPVF VHEDSTTQKT GELRLDSDIP MSKISSDDEV FLAPEMNAFG    50
RQFRDYTDTN SERQKSVEHF YATQHTNQTL DFVQKMRSEY GKLDKMVMNI 100
WECCELSKEV VDESDPDLDE PQIQHLLQSA EAIRKDYPNE DWLHLTALIH 150
DLGKVLTLPQ FGGLPQWAVV GDTFPVGCAF DESNVHHKYF MENPDFNNPK 200
YNTKAGIYSE GCGLENVLMS WGHDDYMYLV AKENGSTLPS PGLFIIRYHS 250
FYPLHKAGAY THLMNEEDKE NLKWLHVFNK YDLYSKSKVH VNVEKVKPYY 300
MSLIKKYFPE NLRW 314
Length:314
Mass (Da):36,544
Last modified:March 1, 2001 - v1
Checksum:iABCF484CC1CDA23C
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti107 – 1071S → L in AAM61190. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB013392 Genomic DNA. Translation: BAB09882.1.
CP002688 Genomic DNA. Translation: AED96791.1.
AY084627 mRNA. Translation: AAM61190.1.
RefSeqiNP_200475.1. NM_125047.2.
UniGeneiAt.29372.

Genome annotation databases

EnsemblPlantsiAT5G56640.1; AT5G56640.1; AT5G56640.
GeneIDi835765.
KEGGiath:AT5G56640.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB013392 Genomic DNA. Translation: BAB09882.1 .
CP002688 Genomic DNA. Translation: AED96791.1 .
AY084627 mRNA. Translation: AAM61190.1 .
RefSeqi NP_200475.1. NM_125047.2.
UniGenei At.29372.

3D structure databases

ProteinModelPortali Q9FJU4.
SMRi Q9FJU4. Positions 53-314.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q9FJU4.
PRIDEi Q9FJU4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G56640.1 ; AT5G56640.1 ; AT5G56640 .
GeneIDi 835765.
KEGGi ath:AT5G56640.

Organism-specific databases

TAIRi AT5G56640.

Phylogenomic databases

eggNOGi NOG135479.
HOGENOMi HOG000163182.
InParanoidi Q9FJU4.
KOi K00469.
OMAi NIWECCE.
PhylomeDBi Q9FJU4.

Enzyme and pathway databases

UniPathwayi UPA00111 ; UER00527 .
BioCyci ARA:AT5G56640-MONOMER.

Gene expression databases

Genevestigatori Q9FJU4.

Family and domain databases

InterProi IPR007828. Inositol_oxygenase.
[Graphical view ]
PANTHERi PTHR12588. PTHR12588. 1 hit.
Pfami PF05153. DUF706. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The inositol oxygenase gene family of Arabidopsis is involved in the biosynthesis of nucleotide sugar precursors for cell-wall matrix polysaccharides."
    Kanter U., Usadel B., Guerineau F., Li Y., Pauly M., Tenhaken R.
    Planta 221:243-254(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
    Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
    DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Myo-inositol oxygenase offers a possible entry point into plant ascorbate biosynthesis."
    Lorence A., Chevone B.I., Mendes P., Nessler C.L.
    Plant Physiol. 134:1200-1205(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiMIOX5_ARATH
AccessioniPrimary (citable) accession number: Q9FJU4
Secondary accession number(s): Q8LFV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: March 1, 2001
Last modified: May 14, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi