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Q9FJU4 (MIOX5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inositol oxygenase 5

EC=1.13.99.1
Alternative name(s):
Myo-inositol oxygenase 5
Short name=AtMIOX5
Short name=MI oxygenase 5
Gene names
Name:MIOX5
Ordered Locus Names:At5g56640
ORF Names:MIK19.9
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length314 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the biosynthesis of UDP-glucuronic acid (UDP-GlcA), providing nucleotide sugars for cell-wall polymers. May be also involved in plant ascorbate biosynthesis. Ref.1 Ref.5

Catalytic activity

Myo-inositol + O2 = D-glucuronate + H2O.

Cofactor

Binds 2 iron ions per subunit By similarity.

Pathway

Polyol metabolism; myo-inositol degradation into D-glucuronate; D-glucuronate from myo-inositol: step 1/1.

Subcellular location

Cytoplasm Probable.

Tissue specificity

Expressed in flowers and siliques. Ref.1

Sequence similarities

Belongs to the myo-inositol oxygenase family.

Ontologies

Keywords
   Biological processAscorbate biosynthesis
   Cellular componentCytoplasm
   LigandIron
Metal-binding
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processL-ascorbic acid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

inositol catabolic process

Inferred from electronic annotation. Source: InterPro

syncytium formation

Inferred from genetic interaction PubMed 19691674. Source: TAIR

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioninositol oxygenase activity

Inferred from electronic annotation. Source: UniProtKB-EC

iron ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 314314Inositol oxygenase 5
PRO_0000079157

Regions

Region112 – 1143Substrate binding By similarity
Region171 – 1722Substrate binding By similarity
Region249 – 2502Substrate binding By similarity

Sites

Metal binding1251Iron 1 By similarity
Metal binding1501Iron 1 By similarity
Metal binding1511Iron 1 By similarity
Metal binding1511Iron 2 By similarity
Metal binding2231Iron 2 By similarity
Metal binding2491Iron 2 By similarity
Metal binding2821Iron 1 By similarity
Binding site541Substrate By similarity
Binding site1541Substrate By similarity

Experimental info

Sequence conflict1071S → L in AAM61190. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q9FJU4 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: ABCF484CC1CDA23C

FASTA31436,544
        10         20         30         40         50         60 
MNISVENPVF VHEDSTTQKT GELRLDSDIP MSKISSDDEV FLAPEMNAFG RQFRDYTDTN 

        70         80         90        100        110        120 
SERQKSVEHF YATQHTNQTL DFVQKMRSEY GKLDKMVMNI WECCELSKEV VDESDPDLDE 

       130        140        150        160        170        180 
PQIQHLLQSA EAIRKDYPNE DWLHLTALIH DLGKVLTLPQ FGGLPQWAVV GDTFPVGCAF 

       190        200        210        220        230        240 
DESNVHHKYF MENPDFNNPK YNTKAGIYSE GCGLENVLMS WGHDDYMYLV AKENGSTLPS 

       250        260        270        280        290        300 
PGLFIIRYHS FYPLHKAGAY THLMNEEDKE NLKWLHVFNK YDLYSKSKVH VNVEKVKPYY 

       310 
MSLIKKYFPE NLRW 

« Hide

References

« Hide 'large scale' references
[1]"The inositol oxygenase gene family of Arabidopsis is involved in the biosynthesis of nucleotide sugar precursors for cell-wall matrix polysaccharides."
Kanter U., Usadel B., Guerineau F., Li Y., Pauly M., Tenhaken R.
Planta 221:243-254(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"Myo-inositol oxygenase offers a possible entry point into plant ascorbate biosynthesis."
Lorence A., Chevone B.I., Mendes P., Nessler C.L.
Plant Physiol. 134:1200-1205(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB013392 Genomic DNA. Translation: BAB09882.1.
CP002688 Genomic DNA. Translation: AED96791.1.
AY084627 mRNA. Translation: AAM61190.1.
RefSeqNP_200475.1. NM_125047.2.
UniGeneAt.29372.

3D structure databases

ProteinModelPortalQ9FJU4.
SMRQ9FJU4. Positions 53-314.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ9FJU4.
PRIDEQ9FJU4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G56640.1; AT5G56640.1; AT5G56640.
GeneID835765.
KEGGath:AT5G56640.

Organism-specific databases

TAIRAT5G56640.

Phylogenomic databases

eggNOGNOG135479.
HOGENOMHOG000163182.
InParanoidQ9FJU4.
KOK00469.
OMANIWECCE.
PhylomeDBQ9FJU4.
ProtClustDBCLSN2687348.

Enzyme and pathway databases

BioCycARA:AT5G56640-MONOMER.
UniPathwayUPA00111; UER00527.

Gene expression databases

GenevestigatorQ9FJU4.

Family and domain databases

InterProIPR007828. Inositol_oxygenase.
[Graphical view]
PANTHERPTHR12588. PTHR12588. 1 hit.
PfamPF05153. DUF706. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMIOX5_ARATH
AccessionPrimary (citable) accession number: Q9FJU4
Secondary accession number(s): Q8LFV4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: March 1, 2001
Last modified: February 19, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names