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Q9FJU4

- MIOX5_ARATH

UniProt

Q9FJU4 - MIOX5_ARATH

Protein

Inositol oxygenase 5

Gene

MIOX5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Involved in the biosynthesis of UDP-glucuronic acid (UDP-GlcA), providing nucleotide sugars for cell-wall polymers. May be also involved in plant ascorbate biosynthesis.2 Publications

    Catalytic activityi

    Myo-inositol + O2 = D-glucuronate + H2O.

    Cofactori

    Binds 2 iron ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei54 – 541SubstrateBy similarity
    Metal bindingi125 – 1251Iron 1By similarity
    Metal bindingi150 – 1501Iron 1By similarity
    Metal bindingi151 – 1511Iron 1By similarity
    Metal bindingi151 – 1511Iron 2By similarity
    Binding sitei154 – 1541SubstrateBy similarity
    Metal bindingi223 – 2231Iron 2By similarity
    Metal bindingi249 – 2491Iron 2By similarity
    Metal bindingi282 – 2821Iron 1By similarity

    GO - Molecular functioni

    1. inositol oxygenase activity Source: UniProtKB-EC
    2. iron ion binding Source: InterPro

    GO - Biological processi

    1. inositol catabolic process Source: InterPro
    2. L-ascorbic acid biosynthetic process Source: UniProtKB-KW
    3. syncytium formation Source: TAIR

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Ascorbate biosynthesis

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G56640-MONOMER.
    UniPathwayiUPA00111; UER00527.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inositol oxygenase 5 (EC:1.13.99.1)
    Alternative name(s):
    Myo-inositol oxygenase 5
    Short name:
    AtMIOX5
    Short name:
    MI oxygenase 5
    Gene namesi
    Name:MIOX5
    Ordered Locus Names:At5g56640
    ORF Names:MIK19.9
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G56640.

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 314314Inositol oxygenase 5PRO_0000079157Add
    BLAST

    Proteomic databases

    PaxDbiQ9FJU4.
    PRIDEiQ9FJU4.

    Expressioni

    Tissue specificityi

    Expressed in flowers and siliques.1 Publication

    Gene expression databases

    GenevestigatoriQ9FJU4.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FJU4.
    SMRiQ9FJU4. Positions 53-314.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni112 – 1143Substrate bindingBy similarity
    Regioni171 – 1722Substrate bindingBy similarity
    Regioni249 – 2502Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the myo-inositol oxygenase family.Curated

    Phylogenomic databases

    eggNOGiNOG135479.
    HOGENOMiHOG000163182.
    InParanoidiQ9FJU4.
    KOiK00469.
    OMAiNIWECCE.
    PhylomeDBiQ9FJU4.

    Family and domain databases

    InterProiIPR007828. Inositol_oxygenase.
    [Graphical view]
    PANTHERiPTHR12588. PTHR12588. 1 hit.
    PfamiPF05153. DUF706. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9FJU4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNISVENPVF VHEDSTTQKT GELRLDSDIP MSKISSDDEV FLAPEMNAFG    50
    RQFRDYTDTN SERQKSVEHF YATQHTNQTL DFVQKMRSEY GKLDKMVMNI 100
    WECCELSKEV VDESDPDLDE PQIQHLLQSA EAIRKDYPNE DWLHLTALIH 150
    DLGKVLTLPQ FGGLPQWAVV GDTFPVGCAF DESNVHHKYF MENPDFNNPK 200
    YNTKAGIYSE GCGLENVLMS WGHDDYMYLV AKENGSTLPS PGLFIIRYHS 250
    FYPLHKAGAY THLMNEEDKE NLKWLHVFNK YDLYSKSKVH VNVEKVKPYY 300
    MSLIKKYFPE NLRW 314
    Length:314
    Mass (Da):36,544
    Last modified:March 1, 2001 - v1
    Checksum:iABCF484CC1CDA23C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti107 – 1071S → L in AAM61190. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB013392 Genomic DNA. Translation: BAB09882.1.
    CP002688 Genomic DNA. Translation: AED96791.1.
    AY084627 mRNA. Translation: AAM61190.1.
    RefSeqiNP_200475.1. NM_125047.2.
    UniGeneiAt.29372.

    Genome annotation databases

    EnsemblPlantsiAT5G56640.1; AT5G56640.1; AT5G56640.
    GeneIDi835765.
    KEGGiath:AT5G56640.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB013392 Genomic DNA. Translation: BAB09882.1 .
    CP002688 Genomic DNA. Translation: AED96791.1 .
    AY084627 mRNA. Translation: AAM61190.1 .
    RefSeqi NP_200475.1. NM_125047.2.
    UniGenei At.29372.

    3D structure databases

    ProteinModelPortali Q9FJU4.
    SMRi Q9FJU4. Positions 53-314.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q9FJU4.
    PRIDEi Q9FJU4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G56640.1 ; AT5G56640.1 ; AT5G56640 .
    GeneIDi 835765.
    KEGGi ath:AT5G56640.

    Organism-specific databases

    TAIRi AT5G56640.

    Phylogenomic databases

    eggNOGi NOG135479.
    HOGENOMi HOG000163182.
    InParanoidi Q9FJU4.
    KOi K00469.
    OMAi NIWECCE.
    PhylomeDBi Q9FJU4.

    Enzyme and pathway databases

    UniPathwayi UPA00111 ; UER00527 .
    BioCyci ARA:AT5G56640-MONOMER.

    Gene expression databases

    Genevestigatori Q9FJU4.

    Family and domain databases

    InterProi IPR007828. Inositol_oxygenase.
    [Graphical view ]
    PANTHERi PTHR12588. PTHR12588. 1 hit.
    Pfami PF05153. DUF706. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The inositol oxygenase gene family of Arabidopsis is involved in the biosynthesis of nucleotide sugar precursors for cell-wall matrix polysaccharides."
      Kanter U., Usadel B., Guerineau F., Li Y., Pauly M., Tenhaken R.
      Planta 221:243-254(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION.
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
      Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
      DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Myo-inositol oxygenase offers a possible entry point into plant ascorbate biosynthesis."
      Lorence A., Chevone B.I., Mendes P., Nessler C.L.
      Plant Physiol. 134:1200-1205(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiMIOX5_ARATH
    AccessioniPrimary (citable) accession number: Q9FJU4
    Secondary accession number(s): Q8LFV4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2005
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3