Peroxidase 65 precursor - Arabidopsis thaliana (Mouse-ear cress)

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Reviewed, UniProtKB/Swiss-Prot Q9FJR1 (PER65_ARATH)

Last modified June 16, 2009. Version 70. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Peroxidase 65
      Short name=Atperox P65
    EC=1.11.1.7
Alternative name(s):
    ATP43

Gene names

Name:	PER65
Synonyms:	P65
Ordered Locus Names:	At5g47000
ORF Names:	MQD22.14

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	Removal of H₂O₂, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
Catalytic activity	Donor + H₂O₂ = oxidized donor + 2 H₂O.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per subunit By similarity. Binds 2 calcium ions per subunit By similarity.
Subcellular location	Secreted By similarity.
Miscellaneous	There are 73 peroxidase genes in A.thaliana.
Sequence similarities	Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.

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Ontologies

Keywords
Biological process	Hydrogen peroxide
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro peroxidase activity Inferred from electronic annotation. Source: UniProtKB-KW xylan 1,4-beta-xylosidase activity Traceable author statement. Source: TAIR
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 28

Potential

Chain

29 – 334

306

Peroxidase 65

PRO_0000023730

Sites

Active site

Proton acceptor By similarity

Metal binding

Calcium 1 By similarity

Metal binding

Calcium 1; via carbonyl oxygen By similarity

Metal binding

Calcium 1 By similarity

Metal binding

Calcium 1 By similarity

Metal binding

201

Iron (heme axial ligand) By similarity

Metal binding

202

Calcium 2 By similarity

Metal binding

250

Calcium 2 By similarity

Metal binding

253

Calcium 2 By similarity

Metal binding

258

Calcium 2 By similarity

Binding site

171

Substrate; via carbonyl oxygen By similarity

Site

Transition state stabilizer By similarity

Amino acid modifications

Glycosylation

174

N-linked (GlcNAc...) Potential

Glycosylation

238

N-linked (GlcNAc...) Potential

Glycosylation

282

N-linked (GlcNAc...) Potential

Glycosylation

294

N-linked (GlcNAc...) Potential

Disulfide bond

42 ↔ 123

By similarity

Disulfide bond

75 ↔ 80

By similarity

Disulfide bond

129 ↔ 326

By similarity

Disulfide bond

208 ↔ 236

By similarity

Experimental info

Sequence conflict

L → F in AAM65654. Ref.3

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Sequences

Sequence

Length

Mass (Da)

Tools

Q9FJR1-1 [UniParc].

Last modified December 6, 2002. Version 2.
Checksum: DC07C78864450E44
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FASTA

334

37,014

        10         20         30         40         50         60 
MSNMQFSRGF NPFVILFCLA VVAPIISADV AILRTDYYQK TCPDFHKIVR EAVTTKQVQQ 

        70         80         90        100        110        120 
PTTAAGTLRL FFHDCFLEGC DASVLIATNS FNKAERDDDL NDSLPGDAFD IVTRIKTALE 

       130        140        150        160        170        180 
LSCPGVVSCA DILAQATRDL VTMVGGPYFD VKLGRKDGFE SKAHKVRGNV PMANQTVPDI 

       190        200        210        220        230        240 
HGIFKKNGFS LREMVALSGA HTIGFSHCKE FSDRLYGSRA DKEINPRFAA ALKDLCKNHT 

       250        260        270        280        290        300 
VDDTIAAFND VMTPGKFDNM YFKNLKRGLG LLASDHILIK DNSTKPFVDL YATNETAFFE 

       310        320        330 
DFARAMEKLG TVGVKGDKDG EVRRRCDHFN NLNV

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones." Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S. DNA Res. 5:203-216(1998) [PubMed: 9734815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Analysis and expression of the class III peroxidase large gene family in Arabidopsis thaliana." Tognolli M., Penel C., Greppin H., Simon P. Gene 288:129-138(2002) [PubMed: 12034502] [Abstract] Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE. Strain: cv. Columbia.

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Cross-references

Sequence databases
	AB013394 Genomic DNA. Translation: BAB10239.1. Different initiation. AY093131 mRNA. Translation: AAM13130.1. BT008821 mRNA. Translation: AAP68260.1. AY088108 mRNA. Translation: AAM65654.1.
IPI	IPI00544962.
RefSeq	NP_568674.1.
UniGene	At.29916
3D structure databases
HSSP	HSSP built from PDB template 1QGJ based on UniProtKB Q39034.
ModBase	Search...
Protein family/group databases
PeroxiBase	231. AtPrx65.
Proteomic databases
PRIDE	Q9FJR1.
Genome annotation databases
GeneID	834746.
GenomeReviews	Gene locus AT5G47000 in contig BA000015_GR.
KEGG	ath:AT5G47000.
NMPDR	fig\|3702.1.peg.26498.
Organism-specific databases
GeneFarm	1924. 61.
TAIR	At5g47000.
Phylogenomic databases
OMA	Q9FJR1. EKPPGQP.
Enzyme and pathway databases
BRENDA	1.11.1.7. 302.
Gene expression databases
ArrayExpress	Q9FJR1.
GermOnline	AT5G47000. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002016. Haem_peroxidase_pln/fun/bac. IPR000823. Peroxidase_pln. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 1 hit. [Graphical view]
PRINTS	PR00458. PEROXIDASE. PR00461. PLPEROXIDASE.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

PER65_ARATH

Accession

Primary (citable) accession number: Q9FJR1
Secondary accession number(s): Q8RWF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2002
Last sequence update:	December 6, 2002
Last modified:	June 16, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents