Q9FJP9 (DHSB3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 3, mitochondrial
EC=1.3.5.1

Alternative name(s):
Iron-sulfur subunit of complex II
Short name=Ip

Gene names

Name:	SDH2-3
Ordered Locus Names:	At5g65165
ORF Names:	MQN23.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) By similarity.
Catalytic activity	Succinate + ubiquinone = fumarate + ubiquinol.
Cofactor	Binds 1 2Fe-2S cluster By similarity. Binds 1 3Fe-4S cluster By similarity. Binds 1 4Fe-4S cluster By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.
Subunit structure	Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b composed of a large and a small subunit By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side By similarity.
Developmental stage	Expressed during seed development. Detected during seed maturation and decreased during germination. Ref.4
Sequence similarities	Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 4Fe-4S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	2Fe-2S 3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial electron transport, succinate to ubiquinone Inferred from sequence or structural similarity Ref.1. Source: TAIR tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from direct assay Ref.1. Source: TAIR succinate dehydrogenase complex Inferred from sequence or structural similarity Ref.1. Source: TAIR
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from sequence or structural similarity Ref.1. Source: TAIR metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW succinate dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC succinate dehydrogenase activity Inferred from sequence or structural similarity Ref.1. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 22	22	Mitochondrion Potential
	Chain	23 – 309	287	Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 3, mitochondrial		PRO_0000247597
Regions
	Domain	69 – 160	92	2Fe-2S ferredoxin-type
	Domain	202 – 232	31	4Fe-4S ferredoxin-type
Sites
	Metal binding	120	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	125	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	128	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	140	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	212	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	215	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	218	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	222	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	270	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	276	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	280	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Binding site	227	1	Ubiquinone; shared with DHSD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9FJP9 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: F69189C234D87A1D	FASTA	309	34,983
	10 20 30 40 50 60 MSSVLRLLGR RICNPAAEKV RLSSSLSGGG DFPILNGHKA AQDLSKDTLK SQDITKEKEG 70 80 90 100 110 120 QHKEVKKEFK IYRWNPDKPN SKPFLQSFFV DLSSCGPMVL DVLQKIKAED DASLSYRRSC 130 140 150 160 170 180 REGICGSCSM NIDGTNTVAC LKPINPNTSK PTIITPLPHM YVIKDLVVDL TNFYQQYKSM 190 200 210 220 230 240 EPWLKTRKPP KDGREHRQSP KDRKKLDGLY ECILCACCTT SCPSYWWNPE EFPGPAALLQ 250 260 270 280 290 300 AYRWISDSRD EYREERLQAI TESETKVYRC RAIKNCTATC PKGLNPASAI LKMKSKHLLS DPLVRTESV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Three different genes encode the iron-sulphur subunit of succinate dehydrogenase in Arabidopsis thaliana." Figueroa P., Leon G., Elorza A., Holuigue L., Jordana X. Plant Mol. Biol. 46:241-250(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Leaf.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones." Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S. DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"A nuclear gene for the iron-sulfur subunit of mitochondrial complex II is specifically expressed during Arabidopsis seed development and germination." Elorza A., Roschzttardtz H., Gomez I., Mouras A., Holuigue L., Araya A., Jordana X. Plant Cell Physiol. 47:14-21(2006) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ278912 mRNA. Translation: CAC19857.1. AB013395 Genomic DNA. Translation: BAB11652.1. CP002688 Genomic DNA. Translation: AED98011.1.
IPI	IPI00528859.
RefSeq	NP_680465.2. NM_148160.2.
UniGene	At.45474.
3D structure databases
ProteinModelPortal	Q9FJP9.
SMR	Q9FJP9. Positions 67-294.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT5G65165.1-P.
Proteomic databases
PaxDb	Q9FJP9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G65165.1; AT5G65165.1; AT5G65165.
GeneID	836640.
KEGG	ath:AT5G65165.
Organism-specific databases
GeneFarm	2172. 175.
TAIR	At5g65165.
Phylogenomic databases
eggNOG	COG0479.
HOGENOM	HOG000160590.
InParanoid	Q9FJP9.
KO	K00235.
OMA	CRTLTKD.
PhylomeDB	Q9FJP9.
ProtClustDB	PLN00129.
Enzyme and pathway databases
UniPathway	UPA00223; UER01006.
Gene expression databases
Genevestigator	Q9FJP9.
Family and domain databases
Gene3D	1.10.1060.10. 1 hit. 3.10.20.30. 1 hit.
InterPro	IPR001041. 2Fe-2S_ferredoxin-type. IPR006058. 2Fe2S_fd_BS. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR012675. Beta-grasp_dom. IPR012285. Fum_reductase_C. IPR009051. Helical_ferredxn. IPR004489. Succ_DH/fum_Rdtase_Fe-S. IPR025192. Succ_DH/fum_Rdtase_N. [Graphical view]
Pfam	PF13085. Fer2_3. 1 hit. [Graphical view]
SUPFAM	SSF54292. Ferredoxin. 1 hit. SSF46548. Helical_ferredxn. 1 hit.
TIGRFAMs	TIGR00384. dhsB. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. False negative. PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHSB3_ARATH

Accession

Primary (citable) accession number: Q9FJP9
Secondary accession number(s): Q9G3L8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program