Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 3, mitochondrial precursor

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Reviewed, UniProtKB/Swiss-Prot Q9FJP9 (DHSB3_ARATH)

Last modified February 9, 2010. Version 61. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 3, mitochondrial
    EC=1.3.5.1
Alternative name(s):
    Iron-sulfur subunit of complex II
      Short name=Ip

Gene names

Name:	SDH2-3
Ordered Locus Names:	At5g65165
ORF Names:	MQN23.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) By similarity.
Catalytic activity	Succinate + ubiquinone = fumarate + ubiquinol.
Cofactor	Binds 1 2Fe-2S cluster By similarity. Binds 1 3Fe-4S cluster By similarity. Binds 1 4Fe-4S cluster By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.
Subunit structure	Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b composed of a large and a small subunit By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side By similarity.
Developmental stage	Expressed during seed development. Detected during seed maturation and decreased during germination. Ref.3
Sequence similarities	Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 4Fe-4S ferredoxin-type domain.

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Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	2Fe-2S 3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW succinate dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 22

Mitochondrion Potential

Chain

23 – 309

287

Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 3, mitochondrial

PRO_0000247597

Regions

Domain

69 – 160

2Fe-2S ferredoxin-type

Domain

202 – 232

4Fe-4S ferredoxin-type

Sites

Metal binding

120

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

125

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

128

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

140

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

212

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

215

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

218

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

222

Iron-sulfur 3 (3Fe-4S) By similarity

Metal binding

270

Iron-sulfur 3 (3Fe-4S) By similarity

Metal binding

276

Iron-sulfur 3 (3Fe-4S) By similarity

Metal binding

280

Iron-sulfur 2 (4Fe-4S) By similarity

Binding site

227

Ubiquinone; shared with DHSD By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

Q9FJP9-1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: F69189C234D87A1D
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FASTA

309

34,983

        10         20         30         40         50         60 
MSSVLRLLGR RICNPAAEKV RLSSSLSGGG DFPILNGHKA AQDLSKDTLK SQDITKEKEG 

        70         80         90        100        110        120 
QHKEVKKEFK IYRWNPDKPN SKPFLQSFFV DLSSCGPMVL DVLQKIKAED DASLSYRRSC 

       130        140        150        160        170        180 
REGICGSCSM NIDGTNTVAC LKPINPNTSK PTIITPLPHM YVIKDLVVDL TNFYQQYKSM 

       190        200        210        220        230        240 
EPWLKTRKPP KDGREHRQSP KDRKKLDGLY ECILCACCTT SCPSYWWNPE EFPGPAALLQ 

       250        260        270        280        290        300 
AYRWISDSRD EYREERLQAI TESETKVYRC RAIKNCTATC PKGLNPASAI LKMKSKHLLS 


DPLVRTESV

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Three different genes encode the iron-sulphur subunit of succinate dehydrogenase in Arabidopsis thaliana." Figueroa P., Leon G., Elorza A., Holuigue L., Jordana X. Plant Mol. Biol. 46:241-250(2001) [PubMed: 11442063] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Leaf.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones." Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S. DNA Res. 5:203-216(1998) [PubMed: 9734815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"A nuclear gene for the iron-sulfur subunit of mitochondrial complex II is specifically expressed during Arabidopsis seed development and germination." Elorza A., Roschzttardtz H., Gomez I., Mouras A., Holuigue L., Araya A., Jordana X. Plant Cell Physiol. 47:14-21(2006) [PubMed: 16249327] [Abstract] Cited for: DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ278912 mRNA. Translation: CAC19857.1. AB013395 Genomic DNA. Translation: BAB11652.1.
IPI	IPI00528859.
RefSeq	NP_680465.2.
UniGene	At.45474
3D structure databases
SMR	Q9FJP9. Positions 67-294.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9FJP9.
Proteomic databases
PRIDE	Q9FJP9.
Genome annotation databases
GeneID	836640.
GenomeReviews	Gene locus AT5G65165 in contig BA000015_GR.
KEGG	ath:AT5G65165.
NMPDR	fig\|3702.1.peg.28594.
Organism-specific databases
GeneFarm	2172. 175.
TAIR	At5g65165.
Phylogenomic databases
eggNOG	KOG3049.
HOGENOM	HBG616382.
InParanoid	Q9FJP9.
OMA	TPLPHMY.
PhylomeDB	Q9FJP9.
Enzyme and pathway databases
BRENDA	1.3.5.1. 302.
Gene expression databases
Genevestigator	Q9FJP9.
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR012675. b-grasp_ferredoxin-like. IPR001041. Ferredoxin. IPR012285. Fum_reductase_C. IPR009051. Helical_ferredxn. IPR004489. Succ_DH/fum_Rdtase_Fe-S. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit. G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
TIGRFAMs	TIGR00384. dhsB. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. False negative. PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

DHSB3_ARATH

Accession

Primary (citable) accession number: Q9FJP9
Secondary accession number(s): Q9G3L8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	March 1, 2001
Last modified:	February 9, 2010
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents