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Protein

Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 3, mitochondrial

Gene

SDH2-3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).By similarity

Catalytic activityi

Succinate + a quinone = fumarate + a quinol.

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster.By similarity
  • [3Fe-4S] clusterBy similarityNote: Binds 1 [3Fe-4S] cluster.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi120 – 1201Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi125 – 1251Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi140 – 1401Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi212 – 2121Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi215 – 2151Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi218 – 2181Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi222 – 2221Iron-sulfur 3 (3Fe-4S)By similarity
Binding sitei227 – 2271Ubiquinone; shared with SdhD subunitBy similarity
Metal bindingi270 – 2701Iron-sulfur 3 (3Fe-4S)By similarity
Metal bindingi276 – 2761Iron-sulfur 3 (3Fe-4S)By similarity
Metal bindingi280 – 2801Iron-sulfur 2 (4Fe-4S)By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 3 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  4. electron carrier activity Source: TAIR
  5. metal ion binding Source: UniProtKB-KW
  6. succinate dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
  7. succinate dehydrogenase activity Source: TAIR

GO - Biological processi

  1. mitochondrial electron transport, succinate to ubiquinone Source: TAIR
  2. tricarboxylic acid cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport, Tricarboxylic acid cycle

Keywords - Ligandi

2Fe-2S, 3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00223; UER01006.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 3, mitochondrial (EC:1.3.5.1)
Alternative name(s):
Iron-sulfur subunit of complex II
Short name:
Ip
Gene namesi
Name:SDH2-3
Ordered Locus Names:At5g65165
ORF Names:MQN23.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G65165.

Subcellular locationi

Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity; Matrix side By similarity

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB-SubCell
  2. mitochondrion Source: TAIR
  3. succinate dehydrogenase complex Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2222MitochondrionSequence AnalysisAdd
BLAST
Chaini23 – 309287Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 3, mitochondrialPRO_0000247597Add
BLAST

Proteomic databases

PaxDbiQ9FJP9.
PRIDEiQ9FJP9.

Expressioni

Developmental stagei

Expressed during seed development. Detected during seed maturation and decreased during germination.1 Publication

Gene expression databases

GenevestigatoriQ9FJP9.

Interactioni

Subunit structurei

Component of complex II composed of eight subunits in plants: four classical SDH subunits SDH1, SDH2, SDH3 and SDH4 (a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit.), as well as four subunits unknown in mitochondria from bacteria and heterotrophic eukaryotes.1 Publication

Protein-protein interaction databases

MINTiMINT-8067094.
STRINGi3702.AT5G65165.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9FJP9.
SMRiQ9FJP9. Positions 67-294.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini69 – 160922Fe-2S ferredoxin-typeAdd
BLAST
Domaini202 – 232314Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 4Fe-4S ferredoxin-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0479.
HOGENOMiHOG000160590.
InParanoidiQ9FJP9.
KOiK00235.
OMAiFCRSAIC.
PhylomeDBiQ9FJP9.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012675. Beta-grasp_dom.
IPR009051. Helical_ferredxn.
IPR004489. Succ_DH/fum_Rdtase_Fe-S.
IPR025192. Succ_DH/fum_Rdtase_N.
[Graphical view]
PfamiPF13085. Fer2_3. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR00384. dhsB. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FJP9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSVLRLLGR RICNPAAEKV RLSSSLSGGG DFPILNGHKA AQDLSKDTLK
60 70 80 90 100
SQDITKEKEG QHKEVKKEFK IYRWNPDKPN SKPFLQSFFV DLSSCGPMVL
110 120 130 140 150
DVLQKIKAED DASLSYRRSC REGICGSCSM NIDGTNTVAC LKPINPNTSK
160 170 180 190 200
PTIITPLPHM YVIKDLVVDL TNFYQQYKSM EPWLKTRKPP KDGREHRQSP
210 220 230 240 250
KDRKKLDGLY ECILCACCTT SCPSYWWNPE EFPGPAALLQ AYRWISDSRD
260 270 280 290 300
EYREERLQAI TESETKVYRC RAIKNCTATC PKGLNPASAI LKMKSKHLLS

DPLVRTESV
Length:309
Mass (Da):34,983
Last modified:February 28, 2001 - v1
Checksum:iF69189C234D87A1D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278912 mRNA. Translation: CAC19857.1.
AB013395 Genomic DNA. Translation: BAB11652.1.
CP002688 Genomic DNA. Translation: AED98011.1.
RefSeqiNP_680465.2. NM_148160.2.
UniGeneiAt.45474.

Genome annotation databases

EnsemblPlantsiAT5G65165.1; AT5G65165.1; AT5G65165.
GeneIDi836640.
KEGGiath:AT5G65165.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278912 mRNA. Translation: CAC19857.1.
AB013395 Genomic DNA. Translation: BAB11652.1.
CP002688 Genomic DNA. Translation: AED98011.1.
RefSeqiNP_680465.2. NM_148160.2.
UniGeneiAt.45474.

3D structure databases

ProteinModelPortaliQ9FJP9.
SMRiQ9FJP9. Positions 67-294.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-8067094.
STRINGi3702.AT5G65165.1-P.

Proteomic databases

PaxDbiQ9FJP9.
PRIDEiQ9FJP9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G65165.1; AT5G65165.1; AT5G65165.
GeneIDi836640.
KEGGiath:AT5G65165.

Organism-specific databases

GeneFarmi2172. 175.
TAIRiAT5G65165.

Phylogenomic databases

eggNOGiCOG0479.
HOGENOMiHOG000160590.
InParanoidiQ9FJP9.
KOiK00235.
OMAiFCRSAIC.
PhylomeDBiQ9FJP9.

Enzyme and pathway databases

UniPathwayiUPA00223; UER01006.

Gene expression databases

GenevestigatoriQ9FJP9.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012675. Beta-grasp_dom.
IPR009051. Helical_ferredxn.
IPR004489. Succ_DH/fum_Rdtase_Fe-S.
IPR025192. Succ_DH/fum_Rdtase_N.
[Graphical view]
PfamiPF13085. Fer2_3. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR00384. dhsB. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Three different genes encode the iron-sulphur subunit of succinate dehydrogenase in Arabidopsis thaliana."
    Figueroa P., Leon G., Elorza A., Holuigue L., Jordana X.
    Plant Mol. Biol. 46:241-250(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
    Tissue: Leaf.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
    Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
    DNA Res. 5:203-216(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "A nuclear gene for the iron-sulfur subunit of mitochondrial complex II is specifically expressed during Arabidopsis seed development and germination."
    Elorza A., Roschzttardtz H., Gomez I., Mouras A., Holuigue L., Araya A., Jordana X.
    Plant Cell Physiol. 47:14-21(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  5. "Mitochondrial cytochrome c oxidase and succinate dehydrogenase complexes contain plant specific subunits."
    Millar A.H., Eubel H., Jansch L., Kruft V., Heazlewood J.L., Braun H.P.
    Plant Mol. Biol. 56:77-90(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT.

Entry informationi

Entry nameiSDHB3_ARATH
AccessioniPrimary (citable) accession number: Q9FJP9
Secondary accession number(s): Q9G3L8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2006
Last sequence update: February 28, 2001
Last modified: February 3, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.