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Q9FJP9 (SDHB3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 3, mitochondrial

EC=1.3.5.1
Alternative name(s):
Iron-sulfur subunit of complex II
Short name=Ip
Gene names
Name:SDH2-3
Ordered Locus Names:At5g65165
ORF Names:MQN23.10
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length309 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) By similarity.

Catalytic activity

Succinate + a quinone = fumarate + a quinol.

Cofactor

Binds 1 2Fe-2S cluster By similarity.

Binds 1 3Fe-4S cluster By similarity.

Binds 1 4Fe-4S cluster By similarity.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.

Subunit structure

Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b composed of a large and a small subunit By similarity.

Subcellular location

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side By similarity.

Developmental stage

Expressed during seed development. Detected during seed maturation and decreased during germination. Ref.4

Sequence similarities

Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.

Contains 1 2Fe-2S ferredoxin-type domain.

Contains 1 4Fe-4S ferredoxin-type domain.

Ontologies

Keywords
   Biological processElectron transport
Transport
Tricarboxylic acid cycle
   Cellular componentMembrane
Mitochondrion
Mitochondrion inner membrane
   DomainTransit peptide
   Ligand2Fe-2S
3Fe-4S
4Fe-4S
Iron
Iron-sulfur
Metal-binding
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmitochondrial electron transport, succinate to ubiquinone

Inferred from sequence or structural similarity Ref.1. Source: TAIR

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentmitochondrial inner membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

mitochondrion

Inferred from direct assay Ref.1. Source: TAIR

succinate dehydrogenase complex

Inferred from sequence or structural similarity Ref.1. Source: TAIR

   Molecular_function2 iron, 2 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-KW

3 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-KW

4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-KW

electron carrier activity

Inferred from sequence or structural similarity Ref.1. Source: TAIR

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

succinate dehydrogenase (ubiquinone) activity

Inferred from electronic annotation. Source: UniProtKB-EC

succinate dehydrogenase activity

Inferred from sequence or structural similarity Ref.1. Source: TAIR

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2222Mitochondrion Potential
Chain23 – 309287Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 3, mitochondrial
PRO_0000247597

Regions

Domain69 – 160922Fe-2S ferredoxin-type
Domain202 – 232314Fe-4S ferredoxin-type

Sites

Metal binding1201Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding1251Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding1281Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding1401Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding2121Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding2151Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding2181Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding2221Iron-sulfur 3 (3Fe-4S) By similarity
Metal binding2701Iron-sulfur 3 (3Fe-4S) By similarity
Metal binding2761Iron-sulfur 3 (3Fe-4S) By similarity
Metal binding2801Iron-sulfur 2 (4Fe-4S) By similarity
Binding site2271Ubiquinone; shared with DHSD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9FJP9 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: F69189C234D87A1D

FASTA30934,983
        10         20         30         40         50         60 
MSSVLRLLGR RICNPAAEKV RLSSSLSGGG DFPILNGHKA AQDLSKDTLK SQDITKEKEG 

        70         80         90        100        110        120 
QHKEVKKEFK IYRWNPDKPN SKPFLQSFFV DLSSCGPMVL DVLQKIKAED DASLSYRRSC 

       130        140        150        160        170        180 
REGICGSCSM NIDGTNTVAC LKPINPNTSK PTIITPLPHM YVIKDLVVDL TNFYQQYKSM 

       190        200        210        220        230        240 
EPWLKTRKPP KDGREHRQSP KDRKKLDGLY ECILCACCTT SCPSYWWNPE EFPGPAALLQ 

       250        260        270        280        290        300 
AYRWISDSRD EYREERLQAI TESETKVYRC RAIKNCTATC PKGLNPASAI LKMKSKHLLS 


DPLVRTESV 

« Hide

References

« Hide 'large scale' references
[1]"Three different genes encode the iron-sulphur subunit of succinate dehydrogenase in Arabidopsis thaliana."
Figueroa P., Leon G., Elorza A., Holuigue L., Jordana X.
Plant Mol. Biol. 46:241-250(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
Tissue: Leaf.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"A nuclear gene for the iron-sulfur subunit of mitochondrial complex II is specifically expressed during Arabidopsis seed development and germination."
Elorza A., Roschzttardtz H., Gomez I., Mouras A., Holuigue L., Araya A., Jordana X.
Plant Cell Physiol. 47:14-21(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ278912 mRNA. Translation: CAC19857.1.
AB013395 Genomic DNA. Translation: BAB11652.1.
CP002688 Genomic DNA. Translation: AED98011.1.
RefSeqNP_680465.2. NM_148160.2.
UniGeneAt.45474.

3D structure databases

ProteinModelPortalQ9FJP9.
SMRQ9FJP9. Positions 67-294.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-8067094.
STRING3702.AT5G65165.1-P.

Proteomic databases

PaxDbQ9FJP9.
PRIDEQ9FJP9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G65165.1; AT5G65165.1; AT5G65165.
GeneID836640.
KEGGath:AT5G65165.

Organism-specific databases

GeneFarm2172. 175.
TAIRAT5G65165.

Phylogenomic databases

eggNOGCOG0479.
HOGENOMHOG000160590.
InParanoidQ9FJP9.
KOK00235.
OMACACCSTQ.
PhylomeDBQ9FJP9.
ProtClustDBPLN00129.

Enzyme and pathway databases

UniPathwayUPA00223; UER01006.

Gene expression databases

GenevestigatorQ9FJP9.

Family and domain databases

Gene3D3.10.20.30. 1 hit.
InterProIPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012675. Beta-grasp_dom.
IPR009051. Helical_ferredxn.
IPR004489. Succ_DH/fum_Rdtase_Fe-S.
IPR025192. Succ_DH/fum_Rdtase_N.
[Graphical view]
PfamPF13085. Fer2_3. 1 hit.
[Graphical view]
SUPFAMSSF46548. SSF46548. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsTIGR00384. dhsB. 1 hit.
PROSITEPS00197. 2FE2S_FER_1. 1 hit.
PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSDHB3_ARATH
AccessionPrimary (citable) accession number: Q9FJP9
Secondary accession number(s): Q9G3L8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names