Reviewed, UniProtKB/Swiss-Prot Q9FJK3 (AGL80_ARATH)

Names and origin

Protein names

Recommended name:
    Agamous-like MADS-box protein AGL80

Gene names

Name:	AGL80
Synonyms:	FEM111
Ordered Locus Names:	At5g48670
ORF Names:	K15N18.16

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probable transcription factor. Controls central cell differentiation during female gametophyte development. Required for the expression of DEMETER and DD46, but not for the expression of FIS2. Ref.1
Subunit structure	Interacts with AGL61 and AGL62. Forms a heterodimer with AGL61. Ref.4 Ref.5 Ref.6 Ref.7
Subcellular location	Nucleus. Ref.1
Tissue specificity	Expressed in the central cell of the female gametophyte and in early endosperm. Also detected in ovaries, young siliques, roots, leaves, stems, young flowers and anthers. Ref.1
Miscellaneous	Plants lacking AGL80 (mutant fem111) have defects in central cell and endosperm development.
Sequence similarities	Contains 1 MADS-box domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Coiled coil
Ligand	DNA-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Ref.1 Inferred from direct assay. Source: TAIR
Molecular function	protein binding Ref.4 Ref.5 Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q2V412	1	EBI-622424,EBI-1238781
AGL61	Q4PSU4	2	EBI-622424,EBI-1237976
AGL62	Q9FKK2	1	EBI-622424,EBI-622140
CALM	P62157	1	EBI-622424,EBI-397403	From a different organism.
CAM1	P25854	1	EBI-622424,EBI-1235664
CAM6	Q03509	1	EBI-622424,EBI-1236097
CAM7	P59220	1	EBI-622424,EBI-1236031
CML10	P30187	1	EBI-622424,EBI-1238722
CML8	O23320	1	EBI-622424,EBI-1237764
CML9	Q9S744	1	EBI-622424,EBI-1236048

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Agamous-like MADS-box protein AGL80		PRO_0000363651
Regions
	Domain	1 – 61	61	MADS-box
	Coiled coil	89 – 114	26	Potential
	Compositional bias	198 – 201	4	Poly-Ser
	Compositional bias	228 – 245	18	Asn-rich
	Compositional bias	275 – 291	17	His-rich

Sequences

	Sequence		Length	Mass (Da)
	Q9FJK3-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 32026DC47F1B3241	FASTA	321	36,825
	10 20 30 40 50 60 MTRKKVKLAY ISNDSSRKAT FKKRKKGLMK KVHELSTLCG ITACAIIYSP YDTNPEVWPS 70 80 90 100 110 120 NSGVQRVVSE FRTLPEMDQH KKMVDQEGFL KQRIAKATET LRRQRKDSRE LEMTEVMFQC 130 140 150 160 170 180 LIGNMEMFHL NIVDLNDLGY MIEQYLKDVN RRIEILRNSG TEIGESSSVA VAASEGNIPM 190 200 210 220 230 240 PNLVATTAPT TTIYEVGSSS SFAAVANFVN PIDLQQFRHP AAQHVGLNEQ PQNLNLNLNQ 250 260 270 280 290 300 NYNQNQEWFM EMMNHPEQMR YQTEQMGYQF MDDNHHNHIH HQPQEHQHQI HDESSNALDA 310 320 ANSSSIIPVT SSSITNKTWF H

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"AGL80 is required for central cell and endosperm development in Arabidopsis." Portereiko M.F., Lloyd A., Steffen J.G., Punwani J.A., Otsuga D., Drews G.N. Plant Cell 18:1862-1872(2006) [PubMed: 16798889] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTANT FEM111, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones." Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S. DNA Res. 5:297-308(1998) [PubMed: 9872454] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W., Redman J.C., Wu H.C., Utterback T., Town C.D. Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Comprehensive interaction map of the Arabidopsis MADS Box transcription factors." de Folter S., Immink R.G.H., Kieffer M., Parenicova L., Henz S.R., Weigel D., Busscher M., Kooiker M., Colombo L., Kater M.M., Davies B., Angenent G.C. Plant Cell 17:1424-1433(2005) [PubMed: 15805477] [Abstract] Cited for: INTERACTION WITH AGL62.
[5]	"The MADS domain protein DIANA acts together with AGAMOUS-LIKE80 to specify the central cell in Arabidopsis ovules." Bemer M., Wolters-Arts M., Grossniklaus U., Angenent G.C. Plant Cell 20:2088-2101(2008) [PubMed: 18713950] [Abstract] Cited for: INTERACTION WITH AGL61.
[6]	"AGL61 interacts with AGL80 and is required for central cell development in Arabidopsis." Steffen J.G., Kang I.-H., Portereiko M.F., Lloyd A., Drews G.N. Plant Physiol. 148:259-268(2008) [PubMed: 18599653] [Abstract] Cited for: INTERACTION WITH AGL61.
[7]	"The AGL62 MADS domain protein regulates cellularization during endosperm development in Arabidopsis." Kang I.-H., Steffen J.G., Portereiko M.F., Lloyd A., Drews G.N. Plant Cell 20:635-647(2008) [PubMed: 18334668] [Abstract] Cited for: INTERACTION WITH AGL62.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ406752 mRNA. Translation: ABD66036.1. AB015468 Genomic DNA. Translation: BAB10700.1. DQ056710 mRNA. Translation: AAY78856.1.
IPI	IPI00518731.
RefSeq	NP_199678.1.
UniGene	At.62436
3D structure databases
HSSP	HSSP built from PDB template 1SRS based on UniProtKB P11831.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9FJK3. 10 interactions.
Genome annotation databases
GeneID	834925.
GenomeReviews	Gene locus AT5G48670 in contig BA000015_GR.
KEGG	ath:AT5G48670.
NMPDR	fig|3702.1.peg.26702.
Organism-specific databases
GeneFarm	3747. 375.
TAIR	At5g48670.
Family and domain databases
InterPro	IPR002100. TF_MADSbox. [Graphical view]
Pfam	PF00319. SRF-TF. 1 hit. [Graphical view]
PRINTS	PR00404. MADSDOMAIN.
SMART	SM00432. MADS. 1 hit. [Graphical view]
PROSITE	PS00350. MADS_BOX_1. False negative. PS50066. MADS_BOX_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGL80_ARATH

Accession

Primary (citable) accession number: Q9FJK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)