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Protein

Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 2

Gene

ACG12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division.

Miscellaneous

There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana.

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

Enzyme regulationi

Regulated by metabolites.

Pathwayi: pentose phosphate pathway

This protein is involved in step 1 of the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glucose-6-phosphate 1-dehydrogenase 3, chloroplastic (At1g24280), Glucose-6-phosphate 1-dehydrogenase 1, chloroplastic (APG1), Glucose-6-phosphate 1-dehydrogenase (At3g27300), Glucose-6-phosphate 1-dehydrogenase (AXX17_At3g29760), Glucose-6-phosphate 1-dehydrogenase (G6PD4), Glucose-6-phosphate 1-dehydrogenase 2, chloroplastic (At5g13110), Glucose-6-phosphate 1-dehydrogenase (AXX17_At1g25530), Glucose-6-phosphate 1-dehydrogenase (At5g35790), Glucose-6-phosphate 1-dehydrogenase (AXX17_At5g33080), Glucose-6-phosphate 1-dehydrogenase (AXX17_At5g33080), Glucose-6-phosphate 1-dehydrogenase (AXX17_At5g12520), Glucose-6-phosphate 1-dehydrogenase (G6PD5), Glucose-6-phosphate 1-dehydrogenase (G6PD5), Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 2 (ACG12), Glucose-6-phosphate 1-dehydrogenase (At1g09420), Glucose-6-phosphate 1-dehydrogenase (AXX17_At5g38480), Glucose-6-phosphate 1-dehydrogenase (AXX17_At1g09330), Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 1 (ACG9), Glucose-6-phosphate 1-dehydrogenase (At5g40760), Glucose-6-phosphate 1-dehydrogenase 4, chloroplastic (At1g09420)
  2. Probable 6-phosphogluconolactonase 5, chloroplastic (EMB2024), Probable 6-phosphogluconolactonase 3 (At5g24420), Probable 6-phosphogluconolactonase 4 (At5g24410), Probable 6-phosphogluconolactonase 1 (At1g13700), Probable 6-phosphogluconolactonase 2 (At3g49360)
  3. 6-phosphogluconate dehydrogenase, decarboxylating (AXX17_At5g39410), 6-phosphogluconate dehydrogenase, decarboxylating (AXX17_At3g01580), 6-phosphogluconate dehydrogenase, decarboxylating 2, chloroplastic (At5g41670), 6-phosphogluconate dehydrogenase, decarboxylating 3 (At3g02360), 6-phosphogluconate dehydrogenase, decarboxylating 1, chloroplastic (At1g64190), 6-phosphogluconate dehydrogenase, decarboxylating (AXX17_At1g57660)
This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei73NADPBy similarity1
Binding sitei182NADP; via carbonyl oxygenBy similarity1
Binding sitei182SubstrateBy similarity1
Binding sitei250SubstrateBy similarity1
Binding sitei269SubstrateBy similarity1
Active sitei274Proton acceptorBy similarity1
Binding sitei360SubstrateBy similarity1
Binding sitei365SubstrateBy similarity1
Binding sitei396SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 45NADPBy similarity8

GO - Molecular functioni

  • glucose-6-phosphate dehydrogenase activity Source: TAIR
  • NADP binding Source: InterPro

GO - Biological processi

  • glucose metabolic process Source: TAIR
  • pentose-phosphate shunt, oxidative branch Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processCarbohydrate metabolism, Glucose metabolism
LigandNADP

Enzyme and pathway databases

BRENDAi1.1.1.49 399
ReactomeiR-ATH-5628897 TP53 Regulates Metabolic Genes
R-ATH-71336 Pentose phosphate pathway (hexose monophosphate shunt)
UniPathwayiUPA00115; UER00408

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 2 (EC:1.1.1.49)
Alternative name(s):
G6PDH6
Short name:
G6PD6
Gene namesi
Name:ACG12
Ordered Locus Names:At5g40760
ORF Names:K1B16.1, MNF13.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G40760
TAIRilocus:2154805 AT5G40760

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000680981 – 515Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 2Add BLAST515

Proteomic databases

PaxDbiQ9FJI5

PTM databases

iPTMnetiQ9FJI5

Expressioni

Gene expression databases

ExpressionAtlasiQ9FJI5 baseline and differential
GenevisibleiQ9FJI5 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT5G40760.1

Structurei

3D structure databases

ProteinModelPortaliQ9FJI5
SMRiQ9FJI5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni212 – 216Substrate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0563 Eukaryota
COG0364 LUCA
HOGENOMiHOG000046192
InParanoidiQ9FJI5
KOiK00036
OMAiVEICVYE
OrthoDBiEOG093607K8
PhylomeDBiQ9FJI5

Family and domain databases

HAMAPiMF_00966 G6PD, 1 hit
InterProiView protein in InterPro
IPR001282 G6P_DH
IPR019796 G6P_DH_AS
IPR022675 G6P_DH_C
IPR022674 G6P_DH_NAD-bd
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR23429 PTHR23429, 1 hit
PfamiView protein in Pfam
PF02781 G6PD_C, 1 hit
PF00479 G6PD_N, 1 hit
PIRSFiPIRSF000110 G6PD, 1 hit
PRINTSiPR00079 G6PDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00871 zwf, 1 hit
PROSITEiView protein in PROSITE
PS00069 G6P_DEHYDROGENASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q9FJI5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSGQWHVEK RSTFRNDSFV REYGIVPETG CLSIIVLGAS GDLAKKKTFP
60 70 80 90 100
ALFNLYRQGF LNPDEVHIFG YARTKISDEE LRDRIRGYLV DEKNAEQAEA
110 120 130 140 150
LSKFLQLIKY VSGPYDAEEG FQRLDKAISE HEISKNSTEG SSRRLFYLAL
160 170 180 190 200
PPSVYPSVCK MIKTCCMNKS DLGGWTRIVV EKPFGKDLES AEQLSSQIGE
210 220 230 240 250
LFDESQIYRI DHYLGKELVQ NMLVLRFANR FFLPLWNRDN IENVQIVFRE
260 270 280 290 300
DFGTEGRGGY FDEYGIIRDI IQNHLLQVLC LVAMEKPISL KPEHIRDEKV
310 320 330 340 350
KVLQSVVPIS DDEVVLGQYE GYRDDDTVPN DSNTPTFATT ILRIHNERWE
360 370 380 390 400
GVPFILKAGK ALNSRKAEIR IQFKDVPGDI FRCQKQGRNE FVIRLQPSEA
410 420 430 440 450
MYMKLTVKQP GLDMNTVQSE LDLSYGQRYQ GVAIPEAYER LILDTIKGDQ
460 470 480 490 500
QHFVRRDELK VAWEIFTPLL HRIDKGEVKS IPYKPGSRGP KEADQLLEKA
510
GYLQTHGYIW IPPTL
Length:515
Mass (Da):59,116
Last modified:March 1, 2001 - v1
Checksum:iE8F7B88A52825C14
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti361A → S in CAB52675 (PubMed:10437832).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010971 mRNA Translation: CAB52675.1
AB015470 Genomic DNA Translation: BAB08837.1
AB009052 Genomic DNA No translation available.
CP002688 Genomic DNA Translation: AED94591.1
CP002688 Genomic DNA Translation: ANM68628.1
BT004633 mRNA Translation: AAO42879.1
PIRiT52610
RefSeqiNP_001330361.1, NM_001344352.1
NP_198892.1, NM_123441.3
UniGeneiAt.23901

Genome annotation databases

EnsemblPlantsiAT5G40760.1; AT5G40760.1; AT5G40760
AT5G40760.2; AT5G40760.2; AT5G40760
GeneIDi834076
GrameneiAT5G40760.1; AT5G40760.1; AT5G40760
AT5G40760.2; AT5G40760.2; AT5G40760
KEGGiath:AT5G40760

Similar proteinsi

Entry informationi

Entry nameiG6PD6_ARATH
AccessioniPrimary (citable) accession number: Q9FJI5
Secondary accession number(s): Q9SUJ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: March 1, 2001
Last modified: April 25, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health