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Protein

Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 2

Gene

ACG12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division.

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

Enzyme regulationi

Regulated by metabolites.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei73 – 731NADPBy similarity
Binding sitei182 – 1821NADP; via carbonyl oxygenBy similarity
Binding sitei182 – 1821SubstrateBy similarity
Binding sitei250 – 2501SubstrateBy similarity
Binding sitei269 – 2691SubstrateBy similarity
Active sitei274 – 2741Proton acceptorBy similarity
Binding sitei360 – 3601SubstrateBy similarity
Binding sitei365 – 3651SubstrateBy similarity
Binding sitei396 – 3961SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi38 – 458NADPBy similarity

GO - Molecular functioni

  • glucose-6-phosphate dehydrogenase activity Source: TAIR
  • NADP binding Source: InterPro

GO - Biological processi

  • glucose metabolic process Source: TAIR
  • pentose-phosphate shunt, oxidative branch Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.1.1.49. 399.
ReactomeiREACT_281719. Pentose phosphate pathway (hexose monophosphate shunt).
REACT_359870. TP53 Regulates Metabolic Genes.
UniPathwayiUPA00115; UER00408.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 2 (EC:1.1.1.49)
Alternative name(s):
G6PDH6
Short name:
G6PD6
Gene namesi
Name:ACG12
Ordered Locus Names:At5g40760
ORF Names:K1B16.1, MNF13.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G40760.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 515515Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 2PRO_0000068098Add
BLAST

Proteomic databases

PaxDbiQ9FJI5.
PRIDEiQ9FJI5.

Expressioni

Gene expression databases

GenevestigatoriQ9FJI5.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9FJI5.
SMRiQ9FJI5. Positions 35-502.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni212 – 2165Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0364.
HOGENOMiHOG000046192.
InParanoidiQ9FJI5.
KOiK00036.
OMAiLYCVTAV.
PhylomeDBiQ9FJI5.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FJI5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSGQWHVEK RSTFRNDSFV REYGIVPETG CLSIIVLGAS GDLAKKKTFP
60 70 80 90 100
ALFNLYRQGF LNPDEVHIFG YARTKISDEE LRDRIRGYLV DEKNAEQAEA
110 120 130 140 150
LSKFLQLIKY VSGPYDAEEG FQRLDKAISE HEISKNSTEG SSRRLFYLAL
160 170 180 190 200
PPSVYPSVCK MIKTCCMNKS DLGGWTRIVV EKPFGKDLES AEQLSSQIGE
210 220 230 240 250
LFDESQIYRI DHYLGKELVQ NMLVLRFANR FFLPLWNRDN IENVQIVFRE
260 270 280 290 300
DFGTEGRGGY FDEYGIIRDI IQNHLLQVLC LVAMEKPISL KPEHIRDEKV
310 320 330 340 350
KVLQSVVPIS DDEVVLGQYE GYRDDDTVPN DSNTPTFATT ILRIHNERWE
360 370 380 390 400
GVPFILKAGK ALNSRKAEIR IQFKDVPGDI FRCQKQGRNE FVIRLQPSEA
410 420 430 440 450
MYMKLTVKQP GLDMNTVQSE LDLSYGQRYQ GVAIPEAYER LILDTIKGDQ
460 470 480 490 500
QHFVRRDELK VAWEIFTPLL HRIDKGEVKS IPYKPGSRGP KEADQLLEKA
510
GYLQTHGYIW IPPTL
Length:515
Mass (Da):59,116
Last modified:March 1, 2001 - v1
Checksum:iE8F7B88A52825C14
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti361 – 3611A → S in CAB52675 (PubMed:10437832).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010971 mRNA. Translation: CAB52675.1.
AB015470 Genomic DNA. Translation: BAB08837.1.
AB009052 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED94591.1.
BT004633 mRNA. Translation: AAO42879.1.
PIRiT52610.
RefSeqiNP_198892.1. NM_123441.2.
UniGeneiAt.23901.

Genome annotation databases

EnsemblPlantsiAT5G40760.1; AT5G40760.1; AT5G40760.
GeneIDi834076.
KEGGiath:AT5G40760.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010971 mRNA. Translation: CAB52675.1.
AB015470 Genomic DNA. Translation: BAB08837.1.
AB009052 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED94591.1.
BT004633 mRNA. Translation: AAO42879.1.
PIRiT52610.
RefSeqiNP_198892.1. NM_123441.2.
UniGeneiAt.23901.

3D structure databases

ProteinModelPortaliQ9FJI5.
SMRiQ9FJI5. Positions 35-502.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiQ9FJI5.
PRIDEiQ9FJI5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G40760.1; AT5G40760.1; AT5G40760.
GeneIDi834076.
KEGGiath:AT5G40760.

Organism-specific databases

TAIRiAT5G40760.

Phylogenomic databases

eggNOGiCOG0364.
HOGENOMiHOG000046192.
InParanoidiQ9FJI5.
KOiK00036.
OMAiLYCVTAV.
PhylomeDBiQ9FJI5.

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408.
BRENDAi1.1.1.49. 399.
ReactomeiREACT_281719. Pentose phosphate pathway (hexose monophosphate shunt).
REACT_359870. TP53 Regulates Metabolic Genes.

Miscellaneous databases

PROiQ9FJI5.

Gene expression databases

GenevestigatoriQ9FJI5.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Evidence for functional convergence of redox regulation in G6PDH isoforms of cyanobacteria and higher plants."
    Wendt U.K., Hauschild R., Lange C., Pietersma M., Wenderoth I., von Schaewen A.
    Plant Mol. Biol. 40:487-494(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones."
    Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S.
    DNA Res. 5:297-308(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones."
    Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 5:41-54(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiG6PD6_ARATH
AccessioniPrimary (citable) accession number: Q9FJI5
Secondary accession number(s): Q9SUJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: March 1, 2001
Last modified: May 27, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.