Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Adenylate kinase isoenzyme 6 homolog

Gene

AAK6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Preferred phosphate donor and acceptor are ATP and AMP, respectively. Has also ATPase activity (By similarity).By similarity1 Publication

Catalytic activityi

ATP + AMP = 2 ADP.UniRule annotation1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei49 – 491AMPUniRule annotation
Binding sitei89 – 891ATPUniRule annotation
Binding sitei115 – 1151ATPUniRule annotation
Binding sitei119 – 1191ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi23 – 286ATPUniRule annotation

GO - Molecular functioni

  • adenylate kinase activity Source: UniProtKB
  • ATPase activity Source: UniProtKB-HAMAP
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

  • developmental vegetative growth Source: UniProtKB
  • multicellular organism development Source: UniProtKB-KW
  • regulation of growth Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Transferase

Keywords - Biological processi

Growth regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G60340-MONOMER.
ReactomeiR-ATH-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate kinase isoenzyme 6 homologUniRule annotation (EC:2.7.4.3UniRule annotation)
Short name:
AK6UniRule annotation
Alternative name(s):
Dual activity adenylate kinase/ATPaseUniRule annotation
Short name:
AK/ATPaseUniRule annotation
Gene namesi
Name:AAK6
Ordered Locus Names:At5g60340
ORF Names:K9B18.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G60340.

Subcellular locationi

  • Nucleus UniRule annotation1 Publication

GO - Cellular componenti

  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Decreased stem growth.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 178178Adenylate kinase isoenzyme 6 homologPRO_0000421171Add
BLAST

Proteomic databases

PaxDbiQ9FJI1.
PRIDEiQ9FJI1.

Interactioni

Subunit structurei

Interacts with RPS14A.1 Publication

Protein-protein interaction databases

BioGridi21400. 8 interactions.
IntActiQ9FJI1. 11 interactions.
STRINGi3702.AT5G60340.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FJI1.
SMRiQ9FJI1. Positions 14-176.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni43 – 6624NMPbindUniRule annotationAdd
BLAST
Regioni118 – 12811LIDUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the adenylate kinase family. AK6 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG3347. Eukaryota.
COG1936. LUCA.
HOGENOMiHOG000224472.
InParanoidiQ9FJI1.
KOiK18532.
OMAiDNVQCEI.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00039. Adenylate_kinase_AK6.
InterProiIPR020618. Adenyl_kinase_AK6.
IPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9FJI1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARRNRGVTR RERPNLLITG TPGTGKSTTA SALAEATNLR YICIGDLVKE
60 70 80 90 100
KEFYHGWDNE LECHFINEDS VIDELDDAMI EGGNIVDYHG CDFFPQRWFD
110 120 130 140 150
RVVVLRTENS VLYDRLTNRG YSGTKLSNNL QCEMYQVLLE EAHDSYDEEI
160 170
VTELQSNTIE DISNNVSTLT DWINAWQP
Length:178
Mass (Da):20,444
Last modified:March 1, 2001 - v1
Checksum:iE0A3446FB10C0F4B
GO
Isoform 2 (identifier: Q9FJI1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     120-127: GYSGTKLS → SDFQIHQM
     128-178: Missing.

Note: No experimental confirmation available.
Show »
Length:127
Mass (Da):14,697
Checksum:iD68ACCE6360E88F3
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei120 – 1278GYSGTKLS → SDFQIHQM in isoform 2. 1 PublicationVSP_045290
Alternative sequencei128 – 17851Missing in isoform 2. 1 PublicationVSP_045291Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015471 Genomic DNA. Translation: BAB10972.1.
CP002688 Genomic DNA. Translation: AED97311.1.
AY079019 mRNA. Translation: AAL84974.1.
AY093748 mRNA. Translation: AAM10372.1.
AK117598 mRNA. Translation: BAC42255.1.
AK318621 mRNA. Translation: BAH56736.1.
RefSeqiNP_200842.2. NM_125427.2. [Q9FJI1-1]
UniGeneiAt.27572.

Genome annotation databases

EnsemblPlantsiAT5G60340.1; AT5G60340.1; AT5G60340. [Q9FJI1-1]
GeneIDi836156.
KEGGiath:AT5G60340.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015471 Genomic DNA. Translation: BAB10972.1.
CP002688 Genomic DNA. Translation: AED97311.1.
AY079019 mRNA. Translation: AAL84974.1.
AY093748 mRNA. Translation: AAM10372.1.
AK117598 mRNA. Translation: BAC42255.1.
AK318621 mRNA. Translation: BAH56736.1.
RefSeqiNP_200842.2. NM_125427.2. [Q9FJI1-1]
UniGeneiAt.27572.

3D structure databases

ProteinModelPortaliQ9FJI1.
SMRiQ9FJI1. Positions 14-176.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21400. 8 interactions.
IntActiQ9FJI1. 11 interactions.
STRINGi3702.AT5G60340.1.

Proteomic databases

PaxDbiQ9FJI1.
PRIDEiQ9FJI1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G60340.1; AT5G60340.1; AT5G60340. [Q9FJI1-1]
GeneIDi836156.
KEGGiath:AT5G60340.

Organism-specific databases

TAIRiAT5G60340.

Phylogenomic databases

eggNOGiKOG3347. Eukaryota.
COG1936. LUCA.
HOGENOMiHOG000224472.
InParanoidiQ9FJI1.
KOiK18532.
OMAiDNVQCEI.

Enzyme and pathway databases

BioCyciARA:AT5G60340-MONOMER.
ReactomeiR-ATH-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Miscellaneous databases

PROiQ9FJI1.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00039. Adenylate_kinase_AK6.
InterProiIPR020618. Adenyl_kinase_AK6.
IPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones."
    Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S.
    DNA Res. 5:297-308(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
    Tissue: Rosette leaf.
  6. "Identification of a novel nuclear-localized adenylate kinase 6 from Arabidopsis thaliana as an essential stem growth factor."
    Feng X., Yang R., Zheng X., Zhang F.
    Plant Physiol. Biochem. 61:180-186(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, INTERACTION WITH RPS14A.

Entry informationi

Entry nameiKAD6_ARATH
AccessioniPrimary (citable) accession number: Q9FJI1
Secondary accession number(s): C0Z207
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2013
Last sequence update: March 1, 2001
Last modified: July 6, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.