Reviewed, UniProtKB/Swiss-Prot Q9FJ80 (P2A14_ARATH)

Names and origin

Protein names

Recommended name:
    F-box protein PP2-A14
Alternative name(s):
    Protein PHLOEM PROTEIN 2-LIKE A14
      Short name=AtPP2-A14
    SKP1-interacting partner 13

Gene names

Name:	PP2A14
Synonyms:	SKIP13
Ordered Locus Names:	At5g52120
ORF Names:	MSG15.23

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins By similarity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Part of a SCF (SKP1-cullin-F-box) protein ligase complex By similarity. Interacts with SKP1A/ASK1 and SPK1B/ASK2.
Subcellular location	Nucleus By similarity.
Domain	The F-box is necessary for the interaction with ASK proteins By similarity.
Sequence similarities	Contains 1 F-box domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Ref.3 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SKP1A	Q39255	2	EBI-604303,EBI-532357
SKP1B	Q9FHW7	2	EBI-604303,EBI-604076

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	F-box protein PP2-A14		PRO_0000272209
Regions
	Domain	18 – 64	47	F-box

Sequences

	Sequence		Length	Mass (Da)
	Q9FJ80-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: BEB684A7A28A6CF3	FASTA	291	33,631
	10 20 30 40 50 60 MGAASSSIVR SEPFAGKLCG LEDVPENCIT AMFMYMEPPE ICLLARVNKS FHRASRSDAV 70 80 90 100 110 120 WEDKLPSNYK FLVRRILEDQ QQVGVKDKLI YRKKEIYARL CRPNLFDTGT KEAWLDKRSG 130 140 150 160 170 180 KVFLAISPKA MKITGIDDRR YWEHISSDES RFGSITYLRQ IWWLEAVGKI RFEFAPGKYS 190 200 210 220 230 240 LLFKIQLGKP IRKCGRKTCS LDQVHGWDIK PVRFQLSTSD GQCAMSERHL DESGRWVYHH 250 260 270 280 290 AGDFVVENQN SPVWVKFSML QIDCTHTKGG LCLDCVIICP FEYRGKYKYS D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones." Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S. DNA Res. 5:297-308(1998) [PubMed: 9872454] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis." Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J., Hellmann H., Estelle M., Somers D.E., Crosby W.L. Plant J. 34:753-767(2003) [PubMed: 12795696] [Abstract] Cited for: INTERACTION WITH SKP1A/ASK1 AND SPK1B/ASK2.
[4]	"Diversity of the superfamily of phloem lectins (phloem protein 2) in angiosperms." Dinant S., Clark A.M., Zhu Y., Vilaine F., Palauqui J.-C., Kusiak C., Thompson G.A. Plant Physiol. 131:114-128(2003) [PubMed: 12529520] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB015478, AB025603 Genomic DNA. Translation: BAB11060.1. AY072088 mRNA. Translation: AAL59911.1. AY096374 mRNA. Translation: AAM20015.1.
IPI	IPI00529837.
RefSeq	NP_200025.1.
UniGene	At.29636
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9FJ80. 6 interactions.
Genome annotation databases
GeneID	835288.
GenomeReviews	Gene locus AT5G52120 in contig BA000015_GR.
KEGG	ath:AT5G52120.
NMPDR	fig|3702.1.peg.27091.
Organism-specific databases
TAIR	At5g52120.
Phylogenomic databases
OMA	Q9FJ80. ENCITAM.
Gene expression databases
ArrayExpress	Q9FJ80.
Family and domain databases
InterPro	IPR001810. F-box. [Graphical view]
Pfam	PF00646. F-box. 1 hit. [Graphical view]
PROSITE	PS50181. FBOX. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2A14_ARATH

Accession

Primary (citable) accession number: Q9FJ80

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)