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Protein

Senescence-specific cysteine protease SAG12

Gene

SAG12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cysteine protease that may have a developmental senescence specific cell death function during apoptosis, heavy metal detoxification, and hypersensitive response.6 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei154 – 1541PROSITE-ProRule annotation
Active sitei289 – 2891PROSITE-ProRule annotation
Active sitei310 – 3101PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • aging Source: TAIR
  • defense response to fungus, incompatible interaction Source: TAIR
  • floral organ senescence Source: UniProtKB
  • leaf senescence Source: UniProtKB
  • plant-type hypersensitive response Source: UniProtKB
  • programmed cell death involved in cell development Source: UniProtKB
  • proteolysis involved in cellular protein catabolic process Source: GO_Central
  • response to auxin Source: UniProtKB
  • response to cytokinin Source: UniProtKB
  • response to fructose Source: UniProtKB
  • response to glucose Source: UniProtKB
  • response to sucrose Source: UniProtKB
  • response to UV-B Source: UniProtKB
  • stress response to copper ion Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Apoptosis, Hypersensitive response, Plant defense

Enzyme and pathway databases

BioCyciARA:AT5G45890-MONOMER.

Protein family/group databases

MEROPSiC01.117.

Names & Taxonomyi

Protein namesi
Recommended name:
Senescence-specific cysteine protease SAG12Curated (EC:3.4.22.-Curated)
Alternative name(s):
Cysteine proteinase SAG12Curated
Protein SENESCENCE-ASSOCIATED GENE 121 Publication
Gene namesi
Name:SAG121 Publication
Ordered Locus Names:At5g45890Imported
ORF Names:K15I22.9Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G45890.

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: GO_Central
  • lysosome Source: GO_Central
  • senescence-associated vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

Pathology & Biotechi

Biotechnological usei

Good molecular marker for age-induced senescence onset measurement.2 Publications

Disruption phenotypei

No visible effect on senescence.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Chaini26 – 346321Senescence-specific cysteine protease SAG12Sequence analysisPRO_0000430524Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi124 – 1241N-linked (GlcNAc...)PROSITE-ProRule annotation
Glycosylationi301 – 3011N-linked (GlcNAc...)PROSITE-ProRule annotation

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9FJ47.
PRIDEiQ9FJ47.
ProMEXiQ9FJ47.

Expressioni

Tissue specificityi

Found in senescent leaves, especially in senescence-associated vacuoles- (SAVs) containing cells (e.g. mesophyll and guard cells), and in senescencing ovules of unfertilised pistils.3 Publications

Developmental stagei

Senescent tissues specific expression (Ref. 6, PubMed:10579486). Detected only after significant visible yellowing (PubMed:9617813). In unfertilised pistils, accumulates transiently shortly after anthesis, and increased again at the end of pistil development (PubMed:21575215). In ovules, first observed at the basal zone of the ovary, and progressively extend acropetally along the ovary (PubMed:21575215).4 Publications

Inductioni

Expression activated by developmentally controlled senescence pathways leading to programmed cell death (PCD), probably by epigenetic regulation via histone H3 deacetylation and by WRKY53 activation (PubMed:10579486, PubMed:10380810, PubMed:18212027, PubMed:18721318, PubMed:19143996). Strongly up-regulated upon abscisic acid treatment (PubMed:9617813). Repressed by cytokinin, auxin (IAA), and sugars (sucrose, glucose and fructose) which can thus repress developmental senescence (PubMed:10579486). The senescence-associated accumulation is salicylic acid- (SA) dependent (PubMed:10972893). Induced slightly in outer leaves by UV-B exposure (PubMed:11432956). Expressed in late stages of heavy-metal- (e.g. copper) and hypersensitive response- (HR) mediated lesions, in chlorotic tissues surrounding the necrosis (PubMed:10380810). The induction by cadmium is nitric oxyde- (NO) dependent and occurs one day before cell death (PubMed:19261736).9 Publications

Gene expression databases

GenevisibleiQ9FJ47. AT.

Interactioni

Protein-protein interaction databases

BioGridi19878. 1 interaction.
IntActiQ9FJ47. 1 interaction.
STRINGi3702.AT5G45890.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FJ47.
SMRiQ9FJ47. Positions 34-346.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1543. Eukaryota.
COG4870. LUCA.
HOGENOMiHOG000230773.
InParanoidiQ9FJ47.
OMAiCTTYLDH.
OrthoDBiEOG09360DAM.
PhylomeDBiQ9FJ47.

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FJ47-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALKHMQIFL FVAIFSSFCF SITLSRPLDN ELIMQKRHIE WMTKHGRVYA
60 70 80 90 100
DVKEENNRYV VFKNNVERIE HLNSIPAGRT FKLAVNQFAD LTNDEFRSMY
110 120 130 140 150
TGFKGVSALS SQSQTKMSPF RYQNVSSGAL PVSVDWRKKG AVTPIKNQGS
160 170 180 190 200
CGCCWAFSAV AAIEGATQIK KGKLISLSEQ QLVDCDTNDF GCEGGLMDTA
210 220 230 240 250
FEHIKATGGL TTESNYPYKG EDATCNSKKT NPKATSITGY EDVPVNDEQA
260 270 280 290 300
LMKAVAHQPV SVGIEGGGFD FQFYSSGVFT GECTTYLDHA VTAIGYGEST
310 320 330 340
NGSKYWIIKN SWGTKWGESG YMRIQKDVKD KQGLCGLAMK ASYPTI
Length:346
Mass (Da):38,234
Last modified:March 1, 2001 - v1
Checksum:i2BE574417D50F167
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti97 – 971R → C in AAC49135 (PubMed:8592746).
Sequence conflicti215 – 2151N → D in AAC49135 (PubMed:8592746).

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37336 Genomic DNA. Translation: AAC49135.1.
AB016870 Genomic DNA. Translation: BAB09317.1.
CP002688 Genomic DNA. Translation: AED95312.1.
AF370131 mRNA. Translation: AAK43946.1.
AY040073 mRNA. Translation: AAK64131.1.
AF083753 mRNA. Translation: AAN60311.1.
RefSeqiNP_568651.1. NM_123957.2.
UniGeneiAt.75256.
At.7710.

Genome annotation databases

EnsemblPlantsiAT5G45890.1; AT5G45890.1; AT5G45890.
GeneIDi834629.
GrameneiAT5G45890.1; AT5G45890.1; AT5G45890.
KEGGiath:AT5G45890.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37336 Genomic DNA. Translation: AAC49135.1.
AB016870 Genomic DNA. Translation: BAB09317.1.
CP002688 Genomic DNA. Translation: AED95312.1.
AF370131 mRNA. Translation: AAK43946.1.
AY040073 mRNA. Translation: AAK64131.1.
AF083753 mRNA. Translation: AAN60311.1.
RefSeqiNP_568651.1. NM_123957.2.
UniGeneiAt.75256.
At.7710.

3D structure databases

ProteinModelPortaliQ9FJ47.
SMRiQ9FJ47. Positions 34-346.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19878. 1 interaction.
IntActiQ9FJ47. 1 interaction.
STRINGi3702.AT5G45890.1.

Protein family/group databases

MEROPSiC01.117.

Proteomic databases

PaxDbiQ9FJ47.
PRIDEiQ9FJ47.
ProMEXiQ9FJ47.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G45890.1; AT5G45890.1; AT5G45890.
GeneIDi834629.
GrameneiAT5G45890.1; AT5G45890.1; AT5G45890.
KEGGiath:AT5G45890.

Organism-specific databases

TAIRiAT5G45890.

Phylogenomic databases

eggNOGiKOG1543. Eukaryota.
COG4870. LUCA.
HOGENOMiHOG000230773.
InParanoidiQ9FJ47.
OMAiCTTYLDH.
OrthoDBiEOG09360DAM.
PhylomeDBiQ9FJ47.

Enzyme and pathway databases

BioCyciARA:AT5G45890-MONOMER.

Miscellaneous databases

PROiQ9FJ47.

Gene expression databases

GenevisibleiQ9FJ47. AT.

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSAG12_ARATH
AccessioniPrimary (citable) accession number: Q9FJ47
Secondary accession number(s): Q38886, Q8H7B7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 29, 2014
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.