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Q9FIZ7 (OPLA_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5-oxoprolinase

EC=3.5.2.9
Alternative name(s):
5-oxo-L-prolinase
Short name=5-OPase
Protein OXOPROLINASE 1
Pyroglutamase
Gene names
Name:OXP1
Ordered Locus Names:At5g37830
ORF Names:K22F20.70
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length1266 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate. Acts in the glutathione degradation pathway. Ref.5

Catalytic activity

ATP + 5-oxo-L-proline + 2 H2O = ADP + phosphate + L-glutamate.

Subcellular location

Cytoplasm Probable.

Tissue specificity

Expressed in roots, stems, leaves, flowers and siliques.

Disruption phenotype

No morphological phenotype, but high accumulation of 5-oxoproline and decreased concentration of glutamate. Ref.5

Sequence similarities

Belongs to the oxoprolinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 126612665-oxoprolinase
PRO_0000380674

Regions

Compositional bias148 – 1525Poly-Asp
Compositional bias1258 – 12636Poly-Gly

Sequences

Sequence LengthMass (Da)Tools
Q9FIZ7 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 77E862B3E4EB7395

FASTA1,266137,531
        10         20         30         40         50         60 
MGTVIEGKLR FCIDRGGTFT DVYAEIPGHS DGHVLKLLSV DPSNYDDAPV EGIRRILEEY 

        70         80         90        100        110        120 
TGKKIPRTSK IPTDKIQWIR MGTTVATNAL LERKGERIAL CVTKGFKDLL QIGNQARPDI 

       130        140        150        160        170        180 
FDLTVAKPSN LYEEVIEVDE RVVLALEDDD DDEGSLIKGV SGEFLRVVKP FDGEGLKPLL 

       190        200        210        220        230        240 
KGLLDKGISC LAVVLMHSYT YPKHEMDVEK LALEMGFRHV SLSSALTPMV RAVPRGLTAT 

       250        260        270        280        290        300 
VDAYLTPVIK EYLSGFISKF DDDLGKVNVL FMQSDGGLAP ESRFSGHKAV LSGPAGGVVG 

       310        320        330        340        350        360 
YSQTLFGLET EKPLIGFDMG GTSTDVSRYD GSYEQVIETQ IAGTIIQAPQ LDINTVAAGG 

       370        380        390        400        410        420 
GSKLKFQFGA FRVGPDSVGA HPGPVCYRKG GELAVTDANL VLGFVIPDYF PSIFGPNEDQ 

       430        440        450        460        470        480 
PLDVAATREA FEKLAGQINI YRKSQDPSAK DMSVEEIAMG FVSVANETMC RPIRQLTEMK 

       490        500        510        520        530        540 
GHETKNHALA CFGGAGPQHA CAIARSLGMK EVLVHRYCGI LSAYGMGLAD VIEDAQEPYS 

       550        560        570        580        590        600 
AVYGPESLSE VFRRETVLLR EVREKLQEQG FGDGNISTET YLNLRYDGTD TAIMVKGKKT 

       610        620        630        640        650        660 
GDGSAFDYAA EFLKLFEQEY GFKLQNRNLL ICDVRVRGIG VTSILKPRAV EAAPVTPKVE 

       670        680        690        700        710        720 
RHYKVYFEGG WHDTPLFKLE NLGFGHEILG PAIIMNGNST VIVEPQCKAI ITKYGNIKIE 

       730        740        750        760        770        780 
VEPATSSVKL AENVADVVQL SIFNHRFMGI AEQMGRTLQR TSISTNIKER LDFSCALFSP 

       790        800        810        820        830        840 
DGGLVANAPH VPVHLGAMSS TVRWQLKHWG ENLNEGDVLV TNHPCAGGSH LPDITVITPV 

       850        860        870        880        890        900 
FDKGKLVFFV ASRGHHAEVG GITPGSMPPF SKAIWEEGAA IKAFKVVEKG VFQEEGIVKL 

       910        920        930        940        950        960 
LQFPSSDETT TKIPGTRRIQ DNLSDLQAQI AANQRGISLI KELIEQYGLG TVQAYMKYVQ 

       970        980        990       1000       1010       1020 
LNAEEAVREM LKSVANRVSS ETPNSRVGNS VTIEEEDYMD DGSIIHLKLT IDADKGEASF 

      1030       1040       1050       1060       1070       1080 
DFTGTSPEVY GNWNAPEAVT SAAVIYCLRC LVNVDIPLNQ GCLAPVEIRI PAGSFLSPSE 

      1090       1100       1110       1120       1130       1140 
KAAVVGGNVL TSQRVTDVVL TAFQACACSQ GCMNNLTFGD DTFGYYETIG GGCGAGPTWN 

      1150       1160       1170       1180       1190       1200 
GTSGVQCHMT NTRMTDPEIF EQRYPVLLHR FGLRENSGGN GLHKGGDGLV REIEFRKPVV 

      1210       1220       1230       1240       1250       1260 
VSILSERRVH SPRGLNGGQN GLRGANYLIT KDKRRIYLGG KNTVHVEAGE ILQILTPGGG 


GFGSNI 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones."
Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S.
DNA Res. 5:297-308(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-963.
Strain: cv. Columbia.
[5]"A gamma-glutamyl transpeptidase-independent pathway of glutathione catabolism to glutamate via 5-oxoproline in Arabidopsis."
Ohkama-Ohtsu N., Oikawa A., Zhao P., Xiang C., Saito K., Oliver D.J.
Plant Physiol. 148:1603-1613(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB016873 Genomic DNA. Translation: BAB10362.1.
CP002688 Genomic DNA. Translation: AED94237.1.
AY102096 mRNA. Translation: AAM26666.1.
BT004510 mRNA. Translation: AAO42756.1.
AK228904 mRNA. Translation: BAF00793.1.
RefSeqNP_198599.1. NM_123142.4.
UniGeneAt.30461.

3D structure databases

ProteinModelPortalQ9FIZ7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid19012. 1 interaction.

Proteomic databases

PaxDbQ9FIZ7.
PRIDEQ9FIZ7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G37830.1; AT5G37830.1; AT5G37830.
GeneID833761.
KEGGath:AT5G37830.

Organism-specific databases

TAIRAT5G37830.

Phylogenomic databases

eggNOGCOG0146.
HOGENOMHOG000047200.
InParanoidQ9FIZ7.
KOK01469.
OMAPLTVTDC.
PhylomeDBQ9FIZ7.
ProtClustDBPLN02666.

Enzyme and pathway databases

BioCycARA:AT5G37830-MONOMER.

Gene expression databases

GenevestigatorQ9FIZ7.

Family and domain databases

InterProIPR008040. Hydant_A_N.
IPR002821. Hydantoinase_A.
IPR003692. Hydantoinase_B.
[Graphical view]
PfamPF05378. Hydant_A_N. 1 hit.
PF01968. Hydantoinase_A. 1 hit.
PF02538. Hydantoinase_B. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ9FIZ7.

Entry information

Entry nameOPLA_ARATH
AccessionPrimary (citable) accession number: Q9FIZ7
Secondary accession number(s): Q0WQ06
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names