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Q9FIZ7

- OPLA_ARATH

UniProt

Q9FIZ7 - OPLA_ARATH

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Protein

5-oxoprolinase

Gene

OXP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate. Acts in the glutathione degradation pathway.1 Publication

Catalytic activityi

ATP + 5-oxo-L-proline + 2 H2O = ADP + phosphate + L-glutamate.

GO - Molecular functioni

  1. 5-oxoprolinase (ATP-hydrolyzing) activity Source: TAIR
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. glutathione catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G37830-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
5-oxoprolinase (EC:3.5.2.9)
Alternative name(s):
5-oxo-L-prolinase
Short name:
5-OPase
Protein OXOPROLINASE 1
Pyroglutamase
Gene namesi
Name:OXP1
Ordered Locus Names:At5g37830
ORF Names:K22F20.70
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G37830.

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: TAIR
  2. cytosol Source: TAIR
  3. plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No morphological phenotype, but high accumulation of 5-oxoproline and decreased concentration of glutamate.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 126612665-oxoprolinasePRO_0000380674Add
BLAST

Proteomic databases

PaxDbiQ9FIZ7.
PRIDEiQ9FIZ7.

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, flowers and siliques.

Gene expression databases

GenevestigatoriQ9FIZ7.

Interactioni

Protein-protein interaction databases

BioGridi19012. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9FIZ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi148 – 1525Poly-Asp
Compositional biasi1258 – 12636Poly-Gly

Sequence similaritiesi

Belongs to the oxoprolinase family.Curated

Phylogenomic databases

eggNOGiCOG0146.
HOGENOMiHOG000047200.
InParanoidiQ9FIZ7.
KOiK01469.
OMAiVEAYMGH.
PhylomeDBiQ9FIZ7.

Family and domain databases

InterProiIPR008040. Hydant_A_N.
IPR002821. Hydantoinase_A.
IPR003692. Hydantoinase_B.
[Graphical view]
PfamiPF05378. Hydant_A_N. 1 hit.
PF01968. Hydantoinase_A. 1 hit.
PF02538. Hydantoinase_B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FIZ7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGTVIEGKLR FCIDRGGTFT DVYAEIPGHS DGHVLKLLSV DPSNYDDAPV
60 70 80 90 100
EGIRRILEEY TGKKIPRTSK IPTDKIQWIR MGTTVATNAL LERKGERIAL
110 120 130 140 150
CVTKGFKDLL QIGNQARPDI FDLTVAKPSN LYEEVIEVDE RVVLALEDDD
160 170 180 190 200
DDEGSLIKGV SGEFLRVVKP FDGEGLKPLL KGLLDKGISC LAVVLMHSYT
210 220 230 240 250
YPKHEMDVEK LALEMGFRHV SLSSALTPMV RAVPRGLTAT VDAYLTPVIK
260 270 280 290 300
EYLSGFISKF DDDLGKVNVL FMQSDGGLAP ESRFSGHKAV LSGPAGGVVG
310 320 330 340 350
YSQTLFGLET EKPLIGFDMG GTSTDVSRYD GSYEQVIETQ IAGTIIQAPQ
360 370 380 390 400
LDINTVAAGG GSKLKFQFGA FRVGPDSVGA HPGPVCYRKG GELAVTDANL
410 420 430 440 450
VLGFVIPDYF PSIFGPNEDQ PLDVAATREA FEKLAGQINI YRKSQDPSAK
460 470 480 490 500
DMSVEEIAMG FVSVANETMC RPIRQLTEMK GHETKNHALA CFGGAGPQHA
510 520 530 540 550
CAIARSLGMK EVLVHRYCGI LSAYGMGLAD VIEDAQEPYS AVYGPESLSE
560 570 580 590 600
VFRRETVLLR EVREKLQEQG FGDGNISTET YLNLRYDGTD TAIMVKGKKT
610 620 630 640 650
GDGSAFDYAA EFLKLFEQEY GFKLQNRNLL ICDVRVRGIG VTSILKPRAV
660 670 680 690 700
EAAPVTPKVE RHYKVYFEGG WHDTPLFKLE NLGFGHEILG PAIIMNGNST
710 720 730 740 750
VIVEPQCKAI ITKYGNIKIE VEPATSSVKL AENVADVVQL SIFNHRFMGI
760 770 780 790 800
AEQMGRTLQR TSISTNIKER LDFSCALFSP DGGLVANAPH VPVHLGAMSS
810 820 830 840 850
TVRWQLKHWG ENLNEGDVLV TNHPCAGGSH LPDITVITPV FDKGKLVFFV
860 870 880 890 900
ASRGHHAEVG GITPGSMPPF SKAIWEEGAA IKAFKVVEKG VFQEEGIVKL
910 920 930 940 950
LQFPSSDETT TKIPGTRRIQ DNLSDLQAQI AANQRGISLI KELIEQYGLG
960 970 980 990 1000
TVQAYMKYVQ LNAEEAVREM LKSVANRVSS ETPNSRVGNS VTIEEEDYMD
1010 1020 1030 1040 1050
DGSIIHLKLT IDADKGEASF DFTGTSPEVY GNWNAPEAVT SAAVIYCLRC
1060 1070 1080 1090 1100
LVNVDIPLNQ GCLAPVEIRI PAGSFLSPSE KAAVVGGNVL TSQRVTDVVL
1110 1120 1130 1140 1150
TAFQACACSQ GCMNNLTFGD DTFGYYETIG GGCGAGPTWN GTSGVQCHMT
1160 1170 1180 1190 1200
NTRMTDPEIF EQRYPVLLHR FGLRENSGGN GLHKGGDGLV REIEFRKPVV
1210 1220 1230 1240 1250
VSILSERRVH SPRGLNGGQN GLRGANYLIT KDKRRIYLGG KNTVHVEAGE
1260
ILQILTPGGG GFGSNI
Length:1,266
Mass (Da):137,531
Last modified:March 1, 2001 - v1
Checksum:i77E862B3E4EB7395
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB016873 Genomic DNA. Translation: BAB10362.1.
CP002688 Genomic DNA. Translation: AED94237.1.
AY102096 mRNA. Translation: AAM26666.1.
BT004510 mRNA. Translation: AAO42756.1.
AK228904 mRNA. Translation: BAF00793.1.
RefSeqiNP_198599.1. NM_123142.4.
UniGeneiAt.30461.

Genome annotation databases

EnsemblPlantsiAT5G37830.1; AT5G37830.1; AT5G37830.
GeneIDi833761.
KEGGiath:AT5G37830.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB016873 Genomic DNA. Translation: BAB10362.1 .
CP002688 Genomic DNA. Translation: AED94237.1 .
AY102096 mRNA. Translation: AAM26666.1 .
BT004510 mRNA. Translation: AAO42756.1 .
AK228904 mRNA. Translation: BAF00793.1 .
RefSeqi NP_198599.1. NM_123142.4.
UniGenei At.30461.

3D structure databases

ProteinModelPortali Q9FIZ7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 19012. 1 interaction.

Proteomic databases

PaxDbi Q9FIZ7.
PRIDEi Q9FIZ7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G37830.1 ; AT5G37830.1 ; AT5G37830 .
GeneIDi 833761.
KEGGi ath:AT5G37830.

Organism-specific databases

TAIRi AT5G37830.

Phylogenomic databases

eggNOGi COG0146.
HOGENOMi HOG000047200.
InParanoidi Q9FIZ7.
KOi K01469.
OMAi VEAYMGH.
PhylomeDBi Q9FIZ7.

Enzyme and pathway databases

BioCyci ARA:AT5G37830-MONOMER.

Miscellaneous databases

PROi Q9FIZ7.

Gene expression databases

Genevestigatori Q9FIZ7.

Family and domain databases

InterProi IPR008040. Hydant_A_N.
IPR002821. Hydantoinase_A.
IPR003692. Hydantoinase_B.
[Graphical view ]
Pfami PF05378. Hydant_A_N. 1 hit.
PF01968. Hydantoinase_A. 1 hit.
PF02538. Hydantoinase_B. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones."
    Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S.
    DNA Res. 5:297-308(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-963.
    Strain: cv. Columbia.
  5. "A gamma-glutamyl transpeptidase-independent pathway of glutathione catabolism to glutamate via 5-oxoproline in Arabidopsis."
    Ohkama-Ohtsu N., Oikawa A., Zhao P., Xiang C., Saito K., Oliver D.J.
    Plant Physiol. 148:1603-1613(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiOPLA_ARATH
AccessioniPrimary (citable) accession number: Q9FIZ7
Secondary accession number(s): Q0WQ06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 1, 2001
Last modified: October 1, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3