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Q9FIZ7

- OPLA_ARATH

UniProt

Q9FIZ7 - OPLA_ARATH

Protein

5-oxoprolinase

Gene

OXP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate. Acts in the glutathione degradation pathway.1 Publication

    Catalytic activityi

    ATP + 5-oxo-L-proline + 2 H2O = ADP + phosphate + L-glutamate.

    GO - Molecular functioni

    1. 5-oxoprolinase (ATP-hydrolyzing) activity Source: TAIR
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. glutathione catabolic process Source: TAIR

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G37830-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-oxoprolinase (EC:3.5.2.9)
    Alternative name(s):
    5-oxo-L-prolinase
    Short name:
    5-OPase
    Protein OXOPROLINASE 1
    Pyroglutamase
    Gene namesi
    Name:OXP1
    Ordered Locus Names:At5g37830
    ORF Names:K22F20.70
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G37830.

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: TAIR
    2. cytosol Source: TAIR
    3. plasmodesma Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    No morphological phenotype, but high accumulation of 5-oxoproline and decreased concentration of glutamate.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 126612665-oxoprolinasePRO_0000380674Add
    BLAST

    Proteomic databases

    PaxDbiQ9FIZ7.
    PRIDEiQ9FIZ7.

    Expressioni

    Tissue specificityi

    Expressed in roots, stems, leaves, flowers and siliques.

    Gene expression databases

    GenevestigatoriQ9FIZ7.

    Interactioni

    Protein-protein interaction databases

    BioGridi19012. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FIZ7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi148 – 1525Poly-Asp
    Compositional biasi1258 – 12636Poly-Gly

    Sequence similaritiesi

    Belongs to the oxoprolinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0146.
    HOGENOMiHOG000047200.
    InParanoidiQ9FIZ7.
    KOiK01469.
    OMAiVEAYMGH.
    PhylomeDBiQ9FIZ7.

    Family and domain databases

    InterProiIPR008040. Hydant_A_N.
    IPR002821. Hydantoinase_A.
    IPR003692. Hydantoinase_B.
    [Graphical view]
    PfamiPF05378. Hydant_A_N. 1 hit.
    PF01968. Hydantoinase_A. 1 hit.
    PF02538. Hydantoinase_B. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9FIZ7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGTVIEGKLR FCIDRGGTFT DVYAEIPGHS DGHVLKLLSV DPSNYDDAPV     50
    EGIRRILEEY TGKKIPRTSK IPTDKIQWIR MGTTVATNAL LERKGERIAL 100
    CVTKGFKDLL QIGNQARPDI FDLTVAKPSN LYEEVIEVDE RVVLALEDDD 150
    DDEGSLIKGV SGEFLRVVKP FDGEGLKPLL KGLLDKGISC LAVVLMHSYT 200
    YPKHEMDVEK LALEMGFRHV SLSSALTPMV RAVPRGLTAT VDAYLTPVIK 250
    EYLSGFISKF DDDLGKVNVL FMQSDGGLAP ESRFSGHKAV LSGPAGGVVG 300
    YSQTLFGLET EKPLIGFDMG GTSTDVSRYD GSYEQVIETQ IAGTIIQAPQ 350
    LDINTVAAGG GSKLKFQFGA FRVGPDSVGA HPGPVCYRKG GELAVTDANL 400
    VLGFVIPDYF PSIFGPNEDQ PLDVAATREA FEKLAGQINI YRKSQDPSAK 450
    DMSVEEIAMG FVSVANETMC RPIRQLTEMK GHETKNHALA CFGGAGPQHA 500
    CAIARSLGMK EVLVHRYCGI LSAYGMGLAD VIEDAQEPYS AVYGPESLSE 550
    VFRRETVLLR EVREKLQEQG FGDGNISTET YLNLRYDGTD TAIMVKGKKT 600
    GDGSAFDYAA EFLKLFEQEY GFKLQNRNLL ICDVRVRGIG VTSILKPRAV 650
    EAAPVTPKVE RHYKVYFEGG WHDTPLFKLE NLGFGHEILG PAIIMNGNST 700
    VIVEPQCKAI ITKYGNIKIE VEPATSSVKL AENVADVVQL SIFNHRFMGI 750
    AEQMGRTLQR TSISTNIKER LDFSCALFSP DGGLVANAPH VPVHLGAMSS 800
    TVRWQLKHWG ENLNEGDVLV TNHPCAGGSH LPDITVITPV FDKGKLVFFV 850
    ASRGHHAEVG GITPGSMPPF SKAIWEEGAA IKAFKVVEKG VFQEEGIVKL 900
    LQFPSSDETT TKIPGTRRIQ DNLSDLQAQI AANQRGISLI KELIEQYGLG 950
    TVQAYMKYVQ LNAEEAVREM LKSVANRVSS ETPNSRVGNS VTIEEEDYMD 1000
    DGSIIHLKLT IDADKGEASF DFTGTSPEVY GNWNAPEAVT SAAVIYCLRC 1050
    LVNVDIPLNQ GCLAPVEIRI PAGSFLSPSE KAAVVGGNVL TSQRVTDVVL 1100
    TAFQACACSQ GCMNNLTFGD DTFGYYETIG GGCGAGPTWN GTSGVQCHMT 1150
    NTRMTDPEIF EQRYPVLLHR FGLRENSGGN GLHKGGDGLV REIEFRKPVV 1200
    VSILSERRVH SPRGLNGGQN GLRGANYLIT KDKRRIYLGG KNTVHVEAGE 1250
    ILQILTPGGG GFGSNI 1266
    Length:1,266
    Mass (Da):137,531
    Last modified:March 1, 2001 - v1
    Checksum:i77E862B3E4EB7395
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB016873 Genomic DNA. Translation: BAB10362.1.
    CP002688 Genomic DNA. Translation: AED94237.1.
    AY102096 mRNA. Translation: AAM26666.1.
    BT004510 mRNA. Translation: AAO42756.1.
    AK228904 mRNA. Translation: BAF00793.1.
    RefSeqiNP_198599.1. NM_123142.4.
    UniGeneiAt.30461.

    Genome annotation databases

    EnsemblPlantsiAT5G37830.1; AT5G37830.1; AT5G37830.
    GeneIDi833761.
    KEGGiath:AT5G37830.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB016873 Genomic DNA. Translation: BAB10362.1 .
    CP002688 Genomic DNA. Translation: AED94237.1 .
    AY102096 mRNA. Translation: AAM26666.1 .
    BT004510 mRNA. Translation: AAO42756.1 .
    AK228904 mRNA. Translation: BAF00793.1 .
    RefSeqi NP_198599.1. NM_123142.4.
    UniGenei At.30461.

    3D structure databases

    ProteinModelPortali Q9FIZ7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 19012. 1 interaction.

    Proteomic databases

    PaxDbi Q9FIZ7.
    PRIDEi Q9FIZ7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G37830.1 ; AT5G37830.1 ; AT5G37830 .
    GeneIDi 833761.
    KEGGi ath:AT5G37830.

    Organism-specific databases

    TAIRi AT5G37830.

    Phylogenomic databases

    eggNOGi COG0146.
    HOGENOMi HOG000047200.
    InParanoidi Q9FIZ7.
    KOi K01469.
    OMAi VEAYMGH.
    PhylomeDBi Q9FIZ7.

    Enzyme and pathway databases

    BioCyci ARA:AT5G37830-MONOMER.

    Miscellaneous databases

    PROi Q9FIZ7.

    Gene expression databases

    Genevestigatori Q9FIZ7.

    Family and domain databases

    InterProi IPR008040. Hydant_A_N.
    IPR002821. Hydantoinase_A.
    IPR003692. Hydantoinase_B.
    [Graphical view ]
    Pfami PF05378. Hydant_A_N. 1 hit.
    PF01968. Hydantoinase_A. 1 hit.
    PF02538. Hydantoinase_B. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones."
      Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S.
      DNA Res. 5:297-308(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-963.
      Strain: cv. Columbia.
    5. "A gamma-glutamyl transpeptidase-independent pathway of glutathione catabolism to glutamate via 5-oxoproline in Arabidopsis."
      Ohkama-Ohtsu N., Oikawa A., Zhao P., Xiang C., Saito K., Oliver D.J.
      Plant Physiol. 148:1603-1613(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiOPLA_ARATH
    AccessioniPrimary (citable) accession number: Q9FIZ7
    Secondary accession number(s): Q0WQ06
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3