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Q9FIQ1

- UBP21_ARATH

UniProt

Q9FIQ1 - UBP21_ARATH

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Protein

Ubiquitin carboxyl-terminal hydrolase 21

Gene
UBP21, At5g46740, MZA15.16
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei172 – 1721Nucleophile By similarity
Active sitei428 – 4281Proton acceptor By similarity

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
  2. ubiquitinyl hydrolase activity Source: InterPro

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciARA:AT5G46740-MONOMER.

Protein family/group databases

MEROPSiC19.A15.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 21 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 21
Short name:
AtUBP21
Ubiquitin thioesterase 21
Ubiquitin-specific-processing protease 21
Gene namesi
Name:UBP21
Ordered Locus Names:At5g46740
ORF Names:MZA15.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G46740.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 732732Ubiquitin carboxyl-terminal hydrolase 21PRO_0000313047Add
BLAST

Proteomic databases

PRIDEiQ9FIQ1.

Expressioni

Gene expression databases

GenevestigatoriQ9FIQ1.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G46740.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9FIQ1.
SMRiQ9FIQ1. Positions 154-466.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini163 – 469307USPAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi86 – 916Poly-Ser

Sequence similaritiesi

Belongs to the peptidase C19 family.
Contains 1 USP domain.

Phylogenomic databases

eggNOGiCOG5077.
HOGENOMiHOG000154757.
InParanoidiQ9FIQ1.
KOiK11855.
OMAiKPFAETS.
PhylomeDBiQ9FIQ1.

Family and domain databases

InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FIQ1-1 [UniParc]FASTAAdd to Basket

« Hide

MAEFSDPPPS NLSSSHKLTK PNQTLDESSP TAPIRDLVTN SLSLSSPIRQ    50
IQALSPAKPD GSSSSPPDKT LNFNPEENRD VNPDESSSSP SDKTLIAPPA 100
QISPVSNNNH LRITNTSDSY LYRPPRRYIE YESDDDELNK MEPTKPLQLS 150
WWYPRIEPTG VGAGLYNSGN TCFIASVLQC FTHTVPLIDS LRSFMYGNPC 200
NCGNEKFCVM QALRDHIELA LRSSGYGINI DRFRDNLTYF SSDFMINHQE 250
DAHEFLQSFL DKLERCCLDP KNQLGSVSSQ DLNIVDNVFG GGLMSTLCCC 300
NCNSVSNTFE PSLGWSLEIE DVNTLWKALE SFTCVEKLED QLTCDNCKEK 350
VTKEKQLRFD KLPPVATFHL KRFTNDGVTM EKIFDHIEFP LELDLSPFMS 400
SNHDPEVSTR YHLYAFVEHI GIRATFGHYS SYVRSAPETW HNFDDSKVTR 450
ISEERVLSRP AYILFYAREG TPWFSSTFEQ LKTVFEATPL HFSPVSVLDN 500
SYESVDNSSK ACNDSVGVSI PDVKWPDSCC QEPKEEVFHS AESSNNEDSS 550
AMIDALGSPQ SEKPFAETSQ QTEPESCPTE NKAYIDKSEK PFAETSQPKE 600
PKPFADRASI DAPLLKVQNQ DISPKRKAGE RATLGGPKLK YQKPNSHQKR 650
QGTFQIQRAH LQTKKQEESR KTKRPLFRSN VAASAPDPKY KNHALSYLNR 700
AQTPRARKLA NALSDSPTKK KKSSNMRRSI KL 732
Length:732
Mass (Da):82,417
Last modified:March 1, 2001 - v1
Checksum:iFDEEDE5B92703F08
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti732 – 7321L → LWRVSY in AAG42759. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF302669 mRNA. Translation: AAG42759.1.
AB016882 Genomic DNA. Translation: BAB08918.1.
CP002688 Genomic DNA. Translation: AED95420.1.
RefSeqiNP_568667.1. NM_124044.1.
UniGeneiAt.7737.

Genome annotation databases

EnsemblPlantsiAT5G46740.1; AT5G46740.1; AT5G46740.
GeneIDi834717.
KEGGiath:AT5G46740.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF302669 mRNA. Translation: AAG42759.1 .
AB016882 Genomic DNA. Translation: BAB08918.1 .
CP002688 Genomic DNA. Translation: AED95420.1 .
RefSeqi NP_568667.1. NM_124044.1.
UniGenei At.7737.

3D structure databases

ProteinModelPortali Q9FIQ1.
SMRi Q9FIQ1. Positions 154-466.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT5G46740.1-P.

Protein family/group databases

MEROPSi C19.A15.

Proteomic databases

PRIDEi Q9FIQ1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G46740.1 ; AT5G46740.1 ; AT5G46740 .
GeneIDi 834717.
KEGGi ath:AT5G46740.

Organism-specific databases

GeneFarmi 2574. 220.
TAIRi AT5G46740.

Phylogenomic databases

eggNOGi COG5077.
HOGENOMi HOG000154757.
InParanoidi Q9FIQ1.
KOi K11855.
OMAi KPFAETS.
PhylomeDBi Q9FIQ1.

Enzyme and pathway databases

BioCyci ARA:AT5G46740-MONOMER.

Gene expression databases

Genevestigatori Q9FIQ1.

Family and domain databases

InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
    Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
    Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE.
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones."
    Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S.
    DNA Res. 5:379-391(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.

Entry informationi

Entry nameiUBP21_ARATH
AccessioniPrimary (citable) accession number: Q9FIQ1
Secondary accession number(s): Q9FPS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2001
Last modified: September 3, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi