Q9FIQ1 (UBP21_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 21
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 21
Short name=AtUBP21
Ubiquitin thioesterase 21
Ubiquitin-specific-processing protease 21

Gene names

Name:	UBP21
Ordered Locus Names:	At5g46740
ORF Names:	MZA15.16

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	732 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Belongs to the peptidase C19 family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 732	732	Ubiquitin carboxyl-terminal hydrolase 21		PRO_0000313047
Regions
	Compositional bias	86 – 91	6	Poly-Ser
Sites
	Active site	172	1	Nucleophile By similarity
	Active site	428	1	Proton acceptor By similarity
Experimental info
	Sequence conflict	732	1	L → LWRVSY in AAG42759. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9FIQ1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: FDEEDE5B92703F08	FASTA	732	82,417
	10 20 30 40 50 60 MAEFSDPPPS NLSSSHKLTK PNQTLDESSP TAPIRDLVTN SLSLSSPIRQ IQALSPAKPD 70 80 90 100 110 120 GSSSSPPDKT LNFNPEENRD VNPDESSSSP SDKTLIAPPA QISPVSNNNH LRITNTSDSY 130 140 150 160 170 180 LYRPPRRYIE YESDDDELNK MEPTKPLQLS WWYPRIEPTG VGAGLYNSGN TCFIASVLQC 190 200 210 220 230 240 FTHTVPLIDS LRSFMYGNPC NCGNEKFCVM QALRDHIELA LRSSGYGINI DRFRDNLTYF 250 260 270 280 290 300 SSDFMINHQE DAHEFLQSFL DKLERCCLDP KNQLGSVSSQ DLNIVDNVFG GGLMSTLCCC 310 320 330 340 350 360 NCNSVSNTFE PSLGWSLEIE DVNTLWKALE SFTCVEKLED QLTCDNCKEK VTKEKQLRFD 370 380 390 400 410 420 KLPPVATFHL KRFTNDGVTM EKIFDHIEFP LELDLSPFMS SNHDPEVSTR YHLYAFVEHI 430 440 450 460 470 480 GIRATFGHYS SYVRSAPETW HNFDDSKVTR ISEERVLSRP AYILFYAREG TPWFSSTFEQ 490 500 510 520 530 540 LKTVFEATPL HFSPVSVLDN SYESVDNSSK ACNDSVGVSI PDVKWPDSCC QEPKEEVFHS 550 560 570 580 590 600 AESSNNEDSS AMIDALGSPQ SEKPFAETSQ QTEPESCPTE NKAYIDKSEK PFAETSQPKE 610 620 630 640 650 660 PKPFADRASI DAPLLKVQNQ DISPKRKAGE RATLGGPKLK YQKPNSHQKR QGTFQIQRAH 670 680 690 700 710 720 LQTKKQEESR KTKRPLFRSN VAASAPDPKY KNHALSYLNR AQTPRARKLA NALSDSPTKK 730 KKSSNMRRSI KL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine." Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D. Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones." Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S. DNA Res. 5:379-391(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF302669 mRNA. Translation: AAG42759.1. AB016882 Genomic DNA. Translation: BAB08918.1. CP002688 Genomic DNA. Translation: AED95420.1.
IPI	IPI00527791.
RefSeq	NP_568667.1. NM_124044.1.
UniGene	At.7737.
3D structure databases
ProteinModelPortal	Q9FIQ1.
SMR	Q9FIQ1. Positions 154-466.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT5G46740.1-P.
Protein family/group databases
MEROPS	C19.A15.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G46740.1; AT5G46740.1; AT5G46740.
GeneID	834717.
KEGG	ath:AT5G46740.
Organism-specific databases
GeneFarm	2574. 220.
TAIR	At5g46740.
Phylogenomic databases
eggNOG	COG5077.
HOGENOM	HOG000154757.
InParanoid	Q9FIQ1.
KO	K11855.
OMA	KPFAETS.
PhylomeDB	Q9FIQ1.
ProtClustDB	CLSN2689489.
Gene expression databases
Genevestigator	Q9FIQ1.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBP21_ARATH

Accession

Primary (citable) accession number: Q9FIQ1
Secondary accession number(s): Q9FPS6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 1, 2001
Last modified:	May 29, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program