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Protein

Ubiquitin carboxyl-terminal hydrolase 21

Gene

UBP21

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei172 – 1721NucleophilePROSITE-ProRule annotation
Active sitei428 – 4281Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciARA:AT5G46740-MONOMER.

Protein family/group databases

MEROPSiC19.A15.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 21 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 21
Short name:
AtUBP21
Ubiquitin thioesterase 21
Ubiquitin-specific-processing protease 21
Gene namesi
Name:UBP21
Ordered Locus Names:At5g46740
ORF Names:MZA15.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G46740.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 732732Ubiquitin carboxyl-terminal hydrolase 21PRO_0000313047Add
BLAST

Proteomic databases

PRIDEiQ9FIQ1.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G46740.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FIQ1.
SMRiQ9FIQ1. Positions 154-466.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini163 – 469307USPAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi86 – 916Poly-Ser

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5077.
HOGENOMiHOG000154757.
InParanoidiQ9FIQ1.
KOiK11855.
OMAiSPIRQIQ.
PhylomeDBiQ9FIQ1.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FIQ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEFSDPPPS NLSSSHKLTK PNQTLDESSP TAPIRDLVTN SLSLSSPIRQ
60 70 80 90 100
IQALSPAKPD GSSSSPPDKT LNFNPEENRD VNPDESSSSP SDKTLIAPPA
110 120 130 140 150
QISPVSNNNH LRITNTSDSY LYRPPRRYIE YESDDDELNK MEPTKPLQLS
160 170 180 190 200
WWYPRIEPTG VGAGLYNSGN TCFIASVLQC FTHTVPLIDS LRSFMYGNPC
210 220 230 240 250
NCGNEKFCVM QALRDHIELA LRSSGYGINI DRFRDNLTYF SSDFMINHQE
260 270 280 290 300
DAHEFLQSFL DKLERCCLDP KNQLGSVSSQ DLNIVDNVFG GGLMSTLCCC
310 320 330 340 350
NCNSVSNTFE PSLGWSLEIE DVNTLWKALE SFTCVEKLED QLTCDNCKEK
360 370 380 390 400
VTKEKQLRFD KLPPVATFHL KRFTNDGVTM EKIFDHIEFP LELDLSPFMS
410 420 430 440 450
SNHDPEVSTR YHLYAFVEHI GIRATFGHYS SYVRSAPETW HNFDDSKVTR
460 470 480 490 500
ISEERVLSRP AYILFYAREG TPWFSSTFEQ LKTVFEATPL HFSPVSVLDN
510 520 530 540 550
SYESVDNSSK ACNDSVGVSI PDVKWPDSCC QEPKEEVFHS AESSNNEDSS
560 570 580 590 600
AMIDALGSPQ SEKPFAETSQ QTEPESCPTE NKAYIDKSEK PFAETSQPKE
610 620 630 640 650
PKPFADRASI DAPLLKVQNQ DISPKRKAGE RATLGGPKLK YQKPNSHQKR
660 670 680 690 700
QGTFQIQRAH LQTKKQEESR KTKRPLFRSN VAASAPDPKY KNHALSYLNR
710 720 730
AQTPRARKLA NALSDSPTKK KKSSNMRRSI KL
Length:732
Mass (Da):82,417
Last modified:March 1, 2001 - v1
Checksum:iFDEEDE5B92703F08
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti732 – 7321L → LWRVSY in AAG42759 (PubMed:11115897).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302669 mRNA. Translation: AAG42759.1.
AB016882 Genomic DNA. Translation: BAB08918.1.
CP002688 Genomic DNA. Translation: AED95420.1.
RefSeqiNP_568667.1. NM_124044.1.
UniGeneiAt.7737.

Genome annotation databases

EnsemblPlantsiAT5G46740.1; AT5G46740.1; AT5G46740.
GeneIDi834717.
KEGGiath:AT5G46740.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302669 mRNA. Translation: AAG42759.1.
AB016882 Genomic DNA. Translation: BAB08918.1.
CP002688 Genomic DNA. Translation: AED95420.1.
RefSeqiNP_568667.1. NM_124044.1.
UniGeneiAt.7737.

3D structure databases

ProteinModelPortaliQ9FIQ1.
SMRiQ9FIQ1. Positions 154-466.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G46740.1.

Protein family/group databases

MEROPSiC19.A15.

Proteomic databases

PRIDEiQ9FIQ1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G46740.1; AT5G46740.1; AT5G46740.
GeneIDi834717.
KEGGiath:AT5G46740.

Organism-specific databases

GeneFarmi2574. 220.
TAIRiAT5G46740.

Phylogenomic databases

eggNOGiCOG5077.
HOGENOMiHOG000154757.
InParanoidiQ9FIQ1.
KOiK11855.
OMAiSPIRQIQ.
PhylomeDBiQ9FIQ1.

Enzyme and pathway databases

BioCyciARA:AT5G46740-MONOMER.

Miscellaneous databases

PROiQ9FIQ1.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
    Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
    Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE.
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones."
    Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S.
    DNA Res. 5:379-391(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.

Entry informationi

Entry nameiUBP21_ARATH
AccessioniPrimary (citable) accession number: Q9FIQ1
Secondary accession number(s): Q9FPS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2001
Last modified: July 22, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.