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Q9FIQ1

- UBP21_ARATH

UniProt

Q9FIQ1 - UBP21_ARATH

Protein

Ubiquitin carboxyl-terminal hydrolase 21

Gene

UBP21

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei172 – 1721NucleophilePROSITE-ProRule annotation
    Active sitei428 – 4281Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. ubiquitinyl hydrolase activity Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    BioCyciARA:AT5G46740-MONOMER.

    Protein family/group databases

    MEROPSiC19.A15.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 21 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 21
    Short name:
    AtUBP21
    Ubiquitin thioesterase 21
    Ubiquitin-specific-processing protease 21
    Gene namesi
    Name:UBP21
    Ordered Locus Names:At5g46740
    ORF Names:MZA15.16
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G46740.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 732732Ubiquitin carboxyl-terminal hydrolase 21PRO_0000313047Add
    BLAST

    Proteomic databases

    PRIDEiQ9FIQ1.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9FIQ1.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT5G46740.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FIQ1.
    SMRiQ9FIQ1. Positions 154-466.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini163 – 469307USPAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi86 – 916Poly-Ser

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiCOG5077.
    HOGENOMiHOG000154757.
    InParanoidiQ9FIQ1.
    KOiK11855.
    OMAiKPFAETS.
    PhylomeDBiQ9FIQ1.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9FIQ1-1 [UniParc]FASTAAdd to Basket

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    MAEFSDPPPS NLSSSHKLTK PNQTLDESSP TAPIRDLVTN SLSLSSPIRQ    50
    IQALSPAKPD GSSSSPPDKT LNFNPEENRD VNPDESSSSP SDKTLIAPPA 100
    QISPVSNNNH LRITNTSDSY LYRPPRRYIE YESDDDELNK MEPTKPLQLS 150
    WWYPRIEPTG VGAGLYNSGN TCFIASVLQC FTHTVPLIDS LRSFMYGNPC 200
    NCGNEKFCVM QALRDHIELA LRSSGYGINI DRFRDNLTYF SSDFMINHQE 250
    DAHEFLQSFL DKLERCCLDP KNQLGSVSSQ DLNIVDNVFG GGLMSTLCCC 300
    NCNSVSNTFE PSLGWSLEIE DVNTLWKALE SFTCVEKLED QLTCDNCKEK 350
    VTKEKQLRFD KLPPVATFHL KRFTNDGVTM EKIFDHIEFP LELDLSPFMS 400
    SNHDPEVSTR YHLYAFVEHI GIRATFGHYS SYVRSAPETW HNFDDSKVTR 450
    ISEERVLSRP AYILFYAREG TPWFSSTFEQ LKTVFEATPL HFSPVSVLDN 500
    SYESVDNSSK ACNDSVGVSI PDVKWPDSCC QEPKEEVFHS AESSNNEDSS 550
    AMIDALGSPQ SEKPFAETSQ QTEPESCPTE NKAYIDKSEK PFAETSQPKE 600
    PKPFADRASI DAPLLKVQNQ DISPKRKAGE RATLGGPKLK YQKPNSHQKR 650
    QGTFQIQRAH LQTKKQEESR KTKRPLFRSN VAASAPDPKY KNHALSYLNR 700
    AQTPRARKLA NALSDSPTKK KKSSNMRRSI KL 732
    Length:732
    Mass (Da):82,417
    Last modified:March 1, 2001 - v1
    Checksum:iFDEEDE5B92703F08
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti732 – 7321L → LWRVSY in AAG42759. (PubMed:11115897)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF302669 mRNA. Translation: AAG42759.1.
    AB016882 Genomic DNA. Translation: BAB08918.1.
    CP002688 Genomic DNA. Translation: AED95420.1.
    RefSeqiNP_568667.1. NM_124044.1.
    UniGeneiAt.7737.

    Genome annotation databases

    EnsemblPlantsiAT5G46740.1; AT5G46740.1; AT5G46740.
    GeneIDi834717.
    KEGGiath:AT5G46740.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF302669 mRNA. Translation: AAG42759.1 .
    AB016882 Genomic DNA. Translation: BAB08918.1 .
    CP002688 Genomic DNA. Translation: AED95420.1 .
    RefSeqi NP_568667.1. NM_124044.1.
    UniGenei At.7737.

    3D structure databases

    ProteinModelPortali Q9FIQ1.
    SMRi Q9FIQ1. Positions 154-466.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT5G46740.1-P.

    Protein family/group databases

    MEROPSi C19.A15.

    Proteomic databases

    PRIDEi Q9FIQ1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G46740.1 ; AT5G46740.1 ; AT5G46740 .
    GeneIDi 834717.
    KEGGi ath:AT5G46740.

    Organism-specific databases

    GeneFarmi 2574. 220.
    TAIRi AT5G46740.

    Phylogenomic databases

    eggNOGi COG5077.
    HOGENOMi HOG000154757.
    InParanoidi Q9FIQ1.
    KOi K11855.
    OMAi KPFAETS.
    PhylomeDBi Q9FIQ1.

    Enzyme and pathway databases

    BioCyci ARA:AT5G46740-MONOMER.

    Gene expression databases

    Genevestigatori Q9FIQ1.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
      Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
      Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE.
      Strain: cv. Columbia.
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones."
      Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S.
      DNA Res. 5:379-391(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiUBP21_ARATH
    AccessioniPrimary (citable) accession number: Q9FIQ1
    Secondary accession number(s): Q9FPS6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3