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Q9FIQ1 (UBP21_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 21

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 21
Short name=AtUBP21
Ubiquitin thioesterase 21
Ubiquitin-specific-processing protease 21
Gene names
Name:UBP21
Ordered Locus Names:At5g46740
ORF Names:MZA15.16
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length732 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 USP domain.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Molecular functionHydrolase
Protease
Thiol protease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncysteine-type peptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 732732Ubiquitin carboxyl-terminal hydrolase 21
PRO_0000313047

Regions

Domain163 – 469307USP
Compositional bias86 – 916Poly-Ser

Sites

Active site1721Nucleophile By similarity
Active site4281Proton acceptor By similarity

Experimental info

Sequence conflict7321L → LWRVSY in AAG42759. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9FIQ1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: FDEEDE5B92703F08

FASTA73282,417
        10         20         30         40         50         60 
MAEFSDPPPS NLSSSHKLTK PNQTLDESSP TAPIRDLVTN SLSLSSPIRQ IQALSPAKPD 

        70         80         90        100        110        120 
GSSSSPPDKT LNFNPEENRD VNPDESSSSP SDKTLIAPPA QISPVSNNNH LRITNTSDSY 

       130        140        150        160        170        180 
LYRPPRRYIE YESDDDELNK MEPTKPLQLS WWYPRIEPTG VGAGLYNSGN TCFIASVLQC 

       190        200        210        220        230        240 
FTHTVPLIDS LRSFMYGNPC NCGNEKFCVM QALRDHIELA LRSSGYGINI DRFRDNLTYF 

       250        260        270        280        290        300 
SSDFMINHQE DAHEFLQSFL DKLERCCLDP KNQLGSVSSQ DLNIVDNVFG GGLMSTLCCC 

       310        320        330        340        350        360 
NCNSVSNTFE PSLGWSLEIE DVNTLWKALE SFTCVEKLED QLTCDNCKEK VTKEKQLRFD 

       370        380        390        400        410        420 
KLPPVATFHL KRFTNDGVTM EKIFDHIEFP LELDLSPFMS SNHDPEVSTR YHLYAFVEHI 

       430        440        450        460        470        480 
GIRATFGHYS SYVRSAPETW HNFDDSKVTR ISEERVLSRP AYILFYAREG TPWFSSTFEQ 

       490        500        510        520        530        540 
LKTVFEATPL HFSPVSVLDN SYESVDNSSK ACNDSVGVSI PDVKWPDSCC QEPKEEVFHS 

       550        560        570        580        590        600 
AESSNNEDSS AMIDALGSPQ SEKPFAETSQ QTEPESCPTE NKAYIDKSEK PFAETSQPKE 

       610        620        630        640        650        660 
PKPFADRASI DAPLLKVQNQ DISPKRKAGE RATLGGPKLK YQKPNSHQKR QGTFQIQRAH 

       670        680        690        700        710        720 
LQTKKQEESR KTKRPLFRSN VAASAPDPKY KNHALSYLNR AQTPRARKLA NALSDSPTKK 

       730 
KKSSNMRRSI KL 

« Hide

References

« Hide 'large scale' references
[1]"The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE.
Strain: cv. Columbia.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones."
Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S.
DNA Res. 5:379-391(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF302669 mRNA. Translation: AAG42759.1.
AB016882 Genomic DNA. Translation: BAB08918.1.
CP002688 Genomic DNA. Translation: AED95420.1.
RefSeqNP_568667.1. NM_124044.1.
UniGeneAt.7737.

3D structure databases

ProteinModelPortalQ9FIQ1.
SMRQ9FIQ1. Positions 154-466.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT5G46740.1-P.

Protein family/group databases

MEROPSC19.A15.

Proteomic databases

PRIDEQ9FIQ1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G46740.1; AT5G46740.1; AT5G46740.
GeneID834717.
KEGGath:AT5G46740.

Organism-specific databases

GeneFarm2574. 220.
TAIRAT5G46740.

Phylogenomic databases

eggNOGCOG5077.
HOGENOMHOG000154757.
InParanoidQ9FIQ1.
KOK11855.
OMAKPFAETS.
PhylomeDBQ9FIQ1.

Enzyme and pathway databases

BioCycARA:AT5G46740-MONOMER.

Gene expression databases

GenevestigatorQ9FIQ1.

Family and domain databases

InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF00443. UCH. 1 hit.
[Graphical view]
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBP21_ARATH
AccessionPrimary (citable) accession number: Q9FIQ1
Secondary accession number(s): Q9FPS6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2001
Last modified: May 14, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names