Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q9FIK7 (THIC2_ARATH)

Last modified November 3, 2009. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Probable acetyl-CoA acetyltransferase, cytosolic 2
    EC=2.3.1.9
Alternative name(s):
    Cytosolic acetoacetyl-CoA thiolase 2
      Short name=Thiolase 2
Gene names
Ordered Locus Names: At5g47720
ORF Names: MCA23.4
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Hide | Top

Protein attributes

Sequence length415 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

Hide | Top

General annotation (Comments)

Catalytic activity

2 acetyl-CoA = CoA + acetoacetyl-CoA.

Subcellular location

Cytoplasm Potential.

Sequence similarities

Belongs to the thiolase family.

Hide | Top

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   LigandMetal-binding
Potassium
   Molecular functionAcyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processmetabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionacetyl-CoA C-acetyltransferase activity

Inferred from electronic annotation. Source: EC

potassium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Hide | Top

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9FIK7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9FIK7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     406-415: Missing.
Note: Derived from EST data. May be due to an intron retention. No experimental confirmation available.

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 415415Probable acetyl-CoA acetyltransferase, cytosolic 2
PRO_0000206412

Regions

Region234 – 2363Coenzyme A binding By similarity

Sites

Active site991Acyl-thioester intermediate By similarity
Active site3611Proton acceptor By similarity
Active site3911Proton acceptor By similarity
Metal binding2561Potassium; via carbonyl oxygen By similarity
Metal binding2571Potassium; via carbonyl oxygen By similarity
Metal binding2591Potassium; via carbonyl oxygen By similarity
Metal binding3571Potassium; via carbonyl oxygen By similarity
Binding site2391Coenzyme A By similarity
Binding site2601Coenzyme A By similarity

Natural variations

Alternative sequence406 – 41510Missing in isoform 2.
VSP_018140

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 04098F25AB18482A

FASTA41543,292
        10         20         30         40         50         60 
MAPPVSDDSL QPRDVCVVGV ARTPIGDFLG SLSSLTATRL GSIAIQAALK RAHVDPALVE 

        70         80         90        100        110        120 
EVFFGNVLTA NLGQAPARQA ALGAGIPYSV ICTTINKVCA AGMKSVMLAS QSIQLGLNDI 

       130        140        150        160        170        180 
VVAGGMESMS NVPKYLPDAR RGSRLGHDTV VDGMMKDGLW DVYNDFGMGV CGEICADQYR 

       190        200        210        220        230        240 
ITREEQDAYA IQSFERGIAA QNTQLFAWEI VPVEVSTGRG RPSVVIDKDE GLGKFDAAKL 

       250        260        270        280        290        300 
KKLRPSFKED GGSVTAGNAS SISDGAAALV LVSGEKALEL GLHVIAKIRG YADAAQAPEL 

       310        320        330        340        350        360 
FTTTPALAIP KAIKRAGLDA SQVDYYEINE AFSVVALANQ KLLGLDPERL NAHGGAVSLG 

       370        380        390        400        410 
HPLGCSGARI LVTLLGVLRA KKGKYGVASI CNGGGGASAL VLEFMSEKTI GYSAL

« Hide

Isoform 2.

Checksum: A5BCC5124637494C
Show »

FASTA40542,242

Hide | Top

References

[1]"Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones."
Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S.
DNA Res. 5:379-391(1998) [PubMed: 10048488] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
+Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases

AB016886 Genomic DNA. Translation: BAB11319.1.
AY088740 mRNA. Translation: AAM67058.1.
IPIIPI00527892.
IPI00531121.
RefSeqNP_199583.1.
NP_851150.1.
NP_974900.1.
NP_974901.2.
UniGeneAt.9393

3D structure databases

HSSPHSSP built from PDB template 1AFW based on UniProtKB P27796.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9FIK7.

Proteomic databases

PRIDEQ9FIK7.

Genome annotation databases

GeneID834823.
GenomeReviewsGene locus AT5G47720 in contig BA000015_GR.
NMPDRfig|3702.1.peg.26584.

Organism-specific databases

TAIRAt5g47720.

Phylogenomic databases

OMARNEVEIS.

Enzyme and pathway databases

BRENDA2.3.1.9. 302.

Gene expression databases

GenevestigatorQ9FIK7.

Family and domain databases

InterProIPR002155. Thiolase.
IPR016038. Thiolase-like_subgr.
[Graphical view]
Gene3DG3DSA:3.40.47.10. Thiolase-like_subgr. 1 hit.
PANTHERPTHR18919. Thiolase. 1 hit.
PfamPF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
[Graphical view]
PIRSFPIRSF000429. Ac-CoA_Ac_transf. 1 hit.
TIGRFAMsTIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITEPS00098. THIOLASE_1. False negative.
PS00737. THIOLASE_2. 1 hit.
PS00099. THIOLASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Hide | Top

Entry information

Entry nameTHIC2_ARATH
AccessionPrimary (citable) accession number: Q9FIK7
Secondary accession number(s): Q3E710, Q3E8F1
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: March 1, 2001
Last modified: November 3, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Hide | Top

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents