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Protein

ATP-dependent 6-phosphofructokinase 2

Gene

PFK2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by AMP.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (MJB21.12), Glucose-6-phosphate isomerase (AXX17_At5g40630), Glucose-6-phosphate isomerase (PGI1 (At4g24620)), Glucose-6-phosphate isomerase, cytosolic (PGIC), Glucose-6-phosphate isomerase (AXX17_At4g28500), Glucose-6-phosphate isomerase 1, chloroplastic (PGI1), Glucose-6-phosphate isomerase (PGIC)
  3. ATP-dependent 6-phosphofructokinase 1 (PFK1), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase 3 (PFK3), ATP-dependent 6-phosphofructokinase 4, chloroplastic (PFK4), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase 2 (PFK2), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 2 (PFP-BETA2), ATP-dependent 6-phosphofructokinase 5, chloroplastic (PFK5), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 1 (PFP-ALPHA1), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), ATP-dependent 6-phosphofructokinase 6 (PFK6), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 2 (PFP-ALPHA2), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 1 (PFP-BETA1), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase 7 (PFK7), ATP-dependent 6-phosphofructokinase (PFK)
  4. Fructose-bisphosphate aldolase 5, cytosolic (FBA5), Fructose-bisphosphate aldolase (FBA6), Fructose-bisphosphate aldolase (FBA4), Fructose-bisphosphate aldolase (AXX17_At4g44320), Fructose-bisphosphate aldolase (AXX17_At4g30610), Fructose-bisphosphate aldolase (FBA2), Fructose-bisphosphate aldolase 1, chloroplastic (FBA1), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 7, cytosolic (FBA7), Fructose-bisphosphate aldolase 3, chloroplastic (FBA3), Fructose-bisphosphate aldolase 6, cytosolic (FBA6), Fructose-bisphosphate aldolase (FBA7), Fructose-bisphosphate aldolase (AXX17_At2g00100), Fructose-bisphosphate aldolase 2, chloroplastic (FBA2), Fructose-bisphosphate aldolase (At2g21330), Fructose-bisphosphate aldolase (AXX17_At2g33200), Fructose-bisphosphate aldolase (AXX17_At3g47340), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (AXX17_At2g16800), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 8, cytosolic (FBA8), Fructose-bisphosphate aldolase (FBA7), Fructose-bisphosphate aldolase (AXX17_At4g30600), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase (AXX17_At3g47340), Fructose-bisphosphate aldolase 4, cytosolic (FBA4)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei86ATP; via amide nitrogenUniRule annotation1
Metal bindingi175Magnesium; catalyticUniRule annotation1
Sitei176Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei205Proton acceptorUniRule annotation1
Binding sitei304SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi149 – 150ATPUniRule annotation2
Nucleotide bindingi174 – 177ATPUniRule annotation4

GO - Molecular functioni

  • 6-phosphofructokinase activity Source: TAIR
  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • fructose 6-phosphate metabolic process Source: InterPro
  • glycolytic process Source: TAIR

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G47810-MONOMER
BRENDAi2.7.1.11 399
ReactomeiR-ATH-6798695 Neutrophil degranulation
R-ATH-70171 Glycolysis
UniPathwayiUPA00109; UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 2UniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFK 2UniRule annotation
Short name:
Phosphofructokinase 2UniRule annotation
Alternative name(s):
Phosphohexokinase 2UniRule annotation
Gene namesi
Name:PFK2UniRule annotation
Ordered Locus Names:At5g47810
ORF Names:MCA23.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G47810
TAIRilocus:2160897 AT5G47810

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003307691 – 444ATP-dependent 6-phosphofructokinase 2Add BLAST444

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei55PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9FIK0
PRIDEiQ9FIK0

Expressioni

Tissue specificityi

Mostly expressed in roots and stems.1 Publication

Gene expression databases

ExpressionAtlasiQ9FIK0 baseline and differential
GenevisibleiQ9FIK0 AT

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

BioGridi20080, 1 interactor
IntActiQ9FIK0, 7 interactors
STRINGi3702.AT5G47810.1

Structurei

3D structure databases

ProteinModelPortaliQ9FIK0
SMRiQ9FIK0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni203 – 205Substrate bindingUniRule annotation3
Regioni248 – 250Substrate bindingUniRule annotation3
Regioni362 – 365Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2440 Eukaryota
COG0205 LUCA
HOGENOMiHOG000016186
InParanoidiQ9FIK0
KOiK00850
OMAiAVDDWHK
OrthoDBiEOG093608ZW
PhylomeDBiQ9FIK0

Family and domain databases

HAMAPiMF_01981 Phosphofructokinase_II_X, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
IPR012004 PyroP-dep_PFK_TP0108
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000534 PPi_PFK_TP0108, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit

Sequencei

Sequence statusi: Complete.

Q9FIK0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAETSIRKL PSLSGLRHRR NPLEDNPYFH PSNGFYITPS DVILAQVAYD
60 70 80 90 100
HSAHSQSRVA YHRAGPRREI MYEPSAVKAA IVTCGGLCPG MNTVIRELVV
110 120 130 140 150
GLWELYGVRE IYGIPAGYRG FYSMKAVKLD PKAVHDWHKK GGTVLATSRG
160 170 180 190 200
GFHLQKIVDA IHLNGYNQVY IIGGDGTMRG AVEIFKEISL RKLEVGITVI
210 220 230 240 250
PKTVDNDVGI IDRSFGFQTA VEMAQEAISA AHVEAESAVN GIGLVKLMGR
260 270 280 290 300
STGHIALHAT LSSRDVDCCL IPEMDFYLEG KGGLFEFLEK RLKERGHAVL
310 320 330 340 350
VVAEGAGQEM IPRNESQKQE RDESGNAVFL DVGVWFKSVL KAWWEREHPD
360 370 380 390 400
ELFTVKYIDP TYMIRAVPAN ATDNLYCTLL AHSAIHGVMA GYTGFVPGPI
410 420 430 440
NGNYAYIPLE EVAQTKNQVN TRDHKWAWVR SVTNQPDFET NVKG
Length:444
Mass (Da):49,182
Last modified:March 1, 2001 - v1
Checksum:i42E78785792A73EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016886 Genomic DNA Translation: BAB11328.1
CP002688 Genomic DNA Translation: AED95574.1
AY056779 mRNA Translation: AAL09725.1
AY090379 mRNA Translation: AAL91281.1
RefSeqiNP_199592.1, NM_124155.3
UniGeneiAt.9400

Genome annotation databases

EnsemblPlantsiAT5G47810.1; AT5G47810.1; AT5G47810
GeneIDi834832
GrameneiAT5G47810.1; AT5G47810.1; AT5G47810
KEGGiath:AT5G47810

Similar proteinsi

Entry informationi

Entry nameiPFKA2_ARATH
AccessioniPrimary (citable) accession number: Q9FIK0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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