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Q9FIK0 (PFKA2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent 6-phosphofructokinase 2

Short name=ATP-PFK 2
Short name=Phosphofructokinase 2
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase 2
Gene names
Name:PFK2
Ordered Locus Names:At5g47810
ORF Names:MCA23.13
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length444 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity. HAMAP-Rule MF_03186

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. Ref.4

Cofactor

Magnesium By similarity. HAMAP-Rule MF_03186

Enzyme regulation

Allosterically activated by AMP By similarity. HAMAP-Rule MF_03186

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_03186

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_03186

Subcellular location

Cytoplasm Ref.4.

Tissue specificity

Mostly expressed in roots and stems. Ref.4

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 444444ATP-dependent 6-phosphofructokinase 2 HAMAP-Rule MF_03186
PRO_0000330769

Regions

Nucleotide binding149 – 1502ATP By similarity
Nucleotide binding174 – 1774ATP By similarity
Region203 – 2053Substrate binding By similarity
Region248 – 2503Substrate binding By similarity
Region362 – 3654Substrate binding By similarity

Sites

Active site2051Proton acceptor By similarity
Metal binding1751Magnesium; catalytic By similarity
Binding site861ATP; via amide nitrogen By similarity
Binding site3041Substrate By similarity
Site1761Important for substrate specificity; cannot use PPi as phosphoryl donor By similarity

Amino acid modifications

Modified residue551Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9FIK0 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 42E78785792A73EC

FASTA44449,182
        10         20         30         40         50         60 
MAAETSIRKL PSLSGLRHRR NPLEDNPYFH PSNGFYITPS DVILAQVAYD HSAHSQSRVA 

        70         80         90        100        110        120 
YHRAGPRREI MYEPSAVKAA IVTCGGLCPG MNTVIRELVV GLWELYGVRE IYGIPAGYRG 

       130        140        150        160        170        180 
FYSMKAVKLD PKAVHDWHKK GGTVLATSRG GFHLQKIVDA IHLNGYNQVY IIGGDGTMRG 

       190        200        210        220        230        240 
AVEIFKEISL RKLEVGITVI PKTVDNDVGI IDRSFGFQTA VEMAQEAISA AHVEAESAVN 

       250        260        270        280        290        300 
GIGLVKLMGR STGHIALHAT LSSRDVDCCL IPEMDFYLEG KGGLFEFLEK RLKERGHAVL 

       310        320        330        340        350        360 
VVAEGAGQEM IPRNESQKQE RDESGNAVFL DVGVWFKSVL KAWWEREHPD ELFTVKYIDP 

       370        380        390        400        410        420 
TYMIRAVPAN ATDNLYCTLL AHSAIHGVMA GYTGFVPGPI NGNYAYIPLE EVAQTKNQVN 

       430        440 
TRDHKWAWVR SVTNQPDFET NVKG 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones."
Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S.
DNA Res. 5:379-391(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Characterisation of the ATP-dependent phosphofructokinase gene family from Arabidopsis thaliana."
Mustroph A., Sonnewald U., Biemelt S.
FEBS Lett. 581:2401-2410(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: CATALYTIC ACTIVITY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB016886 Genomic DNA. Translation: BAB11328.1.
CP002688 Genomic DNA. Translation: AED95574.1.
AY056779 mRNA. Translation: AAL09725.1.
AY090379 mRNA. Translation: AAL91281.1.
RefSeqNP_199592.1. NM_124155.2.
UniGeneAt.9400.

3D structure databases

ProteinModelPortalQ9FIK0.
SMRQ9FIK0. Positions 64-433.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid20080. 1 interaction.
IntActQ9FIK0. 7 interactions.
STRING3702.AT5G47810.1-P.

Proteomic databases

PaxDbQ9FIK0.
PRIDEQ9FIK0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G47810.1; AT5G47810.1; AT5G47810.
GeneID834832.
KEGGath:AT5G47810.

Organism-specific databases

TAIRAT5G47810.

Phylogenomic databases

eggNOGCOG0205.
HOGENOMHOG000016186.
InParanoidQ9FIK0.
KOK00850.
OMAGRWWKRE.
PhylomeDBQ9FIK0.

Enzyme and pathway databases

BioCycARA:AT5G47810-MONOMER.
UniPathwayUPA00109; UER00182.

Gene expression databases

GenevestigatorQ9FIK0.

Family and domain databases

HAMAPMF_01981. Phosphofructokinase_II_X.
InterProIPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFruKinase_TP0108.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
ProtoNetSearch...

Entry information

Entry namePFKA2_ARATH
AccessionPrimary (citable) accession number: Q9FIK0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names