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Q9FIK0

- PFKA2_ARATH

UniProt

Q9FIK0 - PFKA2_ARATH

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Protein
ATP-dependent 6-phosphofructokinase 2
Gene
PFK2, At5g47810, MCA23.13
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.1 Publication

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by AMP By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei86 – 861ATP; via amide nitrogen By similarity
Metal bindingi175 – 1751Magnesium; catalytic By similarity
Sitei176 – 1761Important for substrate specificity; cannot use PPi as phosphoryl donor By similarity
Active sitei205 – 2051Proton acceptor By similarity
Binding sitei304 – 3041Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi149 – 1502ATP By similarity
Nucleotide bindingi174 – 1774ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: TAIR
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. carbohydrate phosphorylation Source: GOC
  2. fructose 6-phosphate metabolic process Source: InterPro
  3. glycolytic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G47810-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 2 (EC:2.7.1.11)
Short name:
ATP-PFK 2
Short name:
Phosphofructokinase 2
Alternative name(s):
Phosphohexokinase 2
Gene namesi
Name:PFK2
Ordered Locus Names:At5g47810
ORF Names:MCA23.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G47810.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
  2. cytosol Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 444444ATP-dependent 6-phosphofructokinase 2UniRule annotation
PRO_0000330769Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei55 – 551Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9FIK0.
PRIDEiQ9FIK0.

Expressioni

Tissue specificityi

Mostly expressed in roots and stems.1 Publication

Gene expression databases

GenevestigatoriQ9FIK0.

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

BioGridi20080. 1 interaction.
IntActiQ9FIK0. 7 interactions.
STRINGi3702.AT5G47810.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9FIK0.
SMRiQ9FIK0. Positions 64-433.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni203 – 2053Substrate binding By similarity
Regioni248 – 2503Substrate binding By similarity
Regioni362 – 3654Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000016186.
InParanoidiQ9FIK0.
KOiK00850.
OMAiGRWWKRE.
PhylomeDBiQ9FIK0.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X.
InterProiIPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFruKinase_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9FIK0-1 [UniParc]FASTAAdd to Basket

« Hide

MAAETSIRKL PSLSGLRHRR NPLEDNPYFH PSNGFYITPS DVILAQVAYD    50
HSAHSQSRVA YHRAGPRREI MYEPSAVKAA IVTCGGLCPG MNTVIRELVV 100
GLWELYGVRE IYGIPAGYRG FYSMKAVKLD PKAVHDWHKK GGTVLATSRG 150
GFHLQKIVDA IHLNGYNQVY IIGGDGTMRG AVEIFKEISL RKLEVGITVI 200
PKTVDNDVGI IDRSFGFQTA VEMAQEAISA AHVEAESAVN GIGLVKLMGR 250
STGHIALHAT LSSRDVDCCL IPEMDFYLEG KGGLFEFLEK RLKERGHAVL 300
VVAEGAGQEM IPRNESQKQE RDESGNAVFL DVGVWFKSVL KAWWEREHPD 350
ELFTVKYIDP TYMIRAVPAN ATDNLYCTLL AHSAIHGVMA GYTGFVPGPI 400
NGNYAYIPLE EVAQTKNQVN TRDHKWAWVR SVTNQPDFET NVKG 444
Length:444
Mass (Da):49,182
Last modified:March 1, 2001 - v1
Checksum:i42E78785792A73EC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB016886 Genomic DNA. Translation: BAB11328.1.
CP002688 Genomic DNA. Translation: AED95574.1.
AY056779 mRNA. Translation: AAL09725.1.
AY090379 mRNA. Translation: AAL91281.1.
RefSeqiNP_199592.1. NM_124155.2.
UniGeneiAt.9400.

Genome annotation databases

EnsemblPlantsiAT5G47810.1; AT5G47810.1; AT5G47810.
GeneIDi834832.
KEGGiath:AT5G47810.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB016886 Genomic DNA. Translation: BAB11328.1 .
CP002688 Genomic DNA. Translation: AED95574.1 .
AY056779 mRNA. Translation: AAL09725.1 .
AY090379 mRNA. Translation: AAL91281.1 .
RefSeqi NP_199592.1. NM_124155.2.
UniGenei At.9400.

3D structure databases

ProteinModelPortali Q9FIK0.
SMRi Q9FIK0. Positions 64-433.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 20080. 1 interaction.
IntActi Q9FIK0. 7 interactions.
STRINGi 3702.AT5G47810.1-P.

Proteomic databases

PaxDbi Q9FIK0.
PRIDEi Q9FIK0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G47810.1 ; AT5G47810.1 ; AT5G47810 .
GeneIDi 834832.
KEGGi ath:AT5G47810.

Organism-specific databases

TAIRi AT5G47810.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000016186.
InParanoidi Q9FIK0.
KOi K00850.
OMAi GRWWKRE.
PhylomeDBi Q9FIK0.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci ARA:AT5G47810-MONOMER.

Gene expression databases

Genevestigatori Q9FIK0.

Family and domain databases

HAMAPi MF_01981. Phosphofructokinase_II_X.
InterProi IPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFruKinase_TP0108.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones."
    Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S.
    DNA Res. 5:379-391(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Characterisation of the ATP-dependent phosphofructokinase gene family from Arabidopsis thaliana."
    Mustroph A., Sonnewald U., Biemelt S.
    FEBS Lett. 581:2401-2410(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiPFKA2_ARATH
AccessioniPrimary (citable) accession number: Q9FIK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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