Reviewed, UniProtKB/Swiss-Prot Q9FIK0 (K6PF2_ARATH)

Names and origin

Protein names

Recommended name:
    6-phosphofructokinase 2
    EC=2.7.1.11
Alternative name(s):
    Phosphofructokinase 2
    Phosphohexokinase 2

Gene names

Name:	PFK2
Ordered Locus Names:	At5g47810
ORF Names:	MCA23.13

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. Ref.3
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Subcellular location	Cytoplasm Ref.3.
Tissue specificity	Mostly expressed in roots and stems. Ref.3
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CAM1	P25854	1	EBI-1238609,EBI-1235664
CAM6	Q03509	1	EBI-1238609,EBI-1236097
CAM7	P59220	1	EBI-1238609,EBI-1236031
CML10	P30187	1	EBI-1238609,EBI-1238722
CML9	Q9S744	1	EBI-1238609,EBI-1236048

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	6-phosphofructokinase 2		PRO_0000330769
Regions
	Nucleotide binding	96 – 100	5	ATP By similarity
	Nucleotide binding	232 – 236	5	ATP By similarity
	Nucleotide binding	250 – 267	18	ATP By similarity
Sites
	Active site	205	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9FIK0-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 42E78785792A73EC	FASTA	444	49,182
	10 20 30 40 50 60 MAAETSIRKL PSLSGLRHRR NPLEDNPYFH PSNGFYITPS DVILAQVAYD HSAHSQSRVA 70 80 90 100 110 120 YHRAGPRREI MYEPSAVKAA IVTCGGLCPG MNTVIRELVV GLWELYGVRE IYGIPAGYRG 130 140 150 160 170 180 FYSMKAVKLD PKAVHDWHKK GGTVLATSRG GFHLQKIVDA IHLNGYNQVY IIGGDGTMRG 190 200 210 220 230 240 AVEIFKEISL RKLEVGITVI PKTVDNDVGI IDRSFGFQTA VEMAQEAISA AHVEAESAVN 250 260 270 280 290 300 GIGLVKLMGR STGHIALHAT LSSRDVDCCL IPEMDFYLEG KGGLFEFLEK RLKERGHAVL 310 320 330 340 350 360 VVAEGAGQEM IPRNESQKQE RDESGNAVFL DVGVWFKSVL KAWWEREHPD ELFTVKYIDP 370 380 390 400 410 420 TYMIRAVPAN ATDNLYCTLL AHSAIHGVMA GYTGFVPGPI NGNYAYIPLE EVAQTKNQVN 430 440 TRDHKWAWVR SVTNQPDFET NVKG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones." Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S. DNA Res. 5:379-391(1998) [PubMed: 10048488] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Characterisation of the ATP-dependent phosphofructokinase gene family from Arabidopsis thaliana." Mustroph A., Sonnewald U., Biemelt S. FEBS Lett. 581:2401-2410(2007) [PubMed: 17485088] [Abstract] Cited for: CATALYTIC ACTIVITY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB016886 Genomic DNA. Translation: BAB11328.1. AY056779 mRNA. Translation: AAL09725.1. AY090379 mRNA. Translation: AAL91281.1.
IPI	IPI00532264.
RefSeq	NP_199592.1.
UniGene	At.9400
3D structure databases
SMR	Q9FIK0. Positions 10-434.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9FIK0. 5 interactions.
STRING	Q9FIK0.
Proteomic databases
PRIDE	Q9FIK0.
Genome annotation databases
GeneID	834832.
GenomeReviews	Gene locus AT5G47810 in contig BA000015_GR.
KEGG	ath:AT5G47810.
NMPDR	fig|3702.1.peg.26596.
Organism-specific databases
TAIR	At5g47810.
Phylogenomic databases
eggNOG	KOG2440.
HOGENOM	HBG316947.
InParanoid	Q9FIK0.
OMA	ANATDNL.
Gene expression databases
Genevestigator	Q9FIK0.
Family and domain databases
InterPro	IPR000023. Phosphofructokinase. IPR012004. PyroP-dep_PFruKinase_TP0108. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
ProtoNet	Search...

Entry information

Entry name

K6PF2_ARATH

Accession

Primary (citable) accession number: Q9FIK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	March 1, 2001
Last modified:	February 9, 2010
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)