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Q9FIK0

- PFKA2_ARATH

UniProt

Q9FIK0 - PFKA2_ARATH

Protein

ATP-dependent 6-phosphofructokinase 2

Gene

PFK2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.1 PublicationUniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by AMP.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei86 – 861ATP; via amide nitrogenUniRule annotation
    Metal bindingi175 – 1751Magnesium; catalyticUniRule annotation
    Sitei176 – 1761Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
    Active sitei205 – 2051Proton acceptorUniRule annotation
    Binding sitei304 – 3041SubstrateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi149 – 1502ATPUniRule annotation
    Nucleotide bindingi174 – 1774ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: TAIR
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. carbohydrate phosphorylation Source: GOC
    2. fructose 6-phosphate metabolic process Source: InterPro
    3. glycolytic process Source: TAIR

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G47810-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinase 2UniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFK 2UniRule annotation
    Short name:
    Phosphofructokinase 2UniRule annotation
    Alternative name(s):
    Phosphohexokinase 2UniRule annotation
    Gene namesi
    Name:PFK2UniRule annotation
    Ordered Locus Names:At5g47810
    ORF Names:MCA23.13
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G47810.

    Subcellular locationi

    Cytoplasm 1 PublicationUniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro
    2. cytosol Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 444444ATP-dependent 6-phosphofructokinase 2PRO_0000330769Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei55 – 551PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9FIK0.
    PRIDEiQ9FIK0.

    Expressioni

    Tissue specificityi

    Mostly expressed in roots and stems.1 Publication

    Gene expression databases

    GenevestigatoriQ9FIK0.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    BioGridi20080. 1 interaction.
    IntActiQ9FIK0. 7 interactions.
    STRINGi3702.AT5G47810.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FIK0.
    SMRiQ9FIK0. Positions 64-433.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni203 – 2053Substrate bindingUniRule annotation
    Regioni248 – 2503Substrate bindingUniRule annotation
    Regioni362 – 3654Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    HOGENOMiHOG000016186.
    InParanoidiQ9FIK0.
    KOiK00850.
    OMAiGRWWKRE.
    PhylomeDBiQ9FIK0.

    Family and domain databases

    HAMAPiMF_01981. Phosphofructokinase_II_X.
    InterProiIPR022953. Phosphofructokinase.
    IPR000023. Phosphofructokinase_dom.
    IPR012004. PyroP-dep_PFruKinase_TP0108.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9FIK0-1 [UniParc]FASTAAdd to Basket

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    MAAETSIRKL PSLSGLRHRR NPLEDNPYFH PSNGFYITPS DVILAQVAYD    50
    HSAHSQSRVA YHRAGPRREI MYEPSAVKAA IVTCGGLCPG MNTVIRELVV 100
    GLWELYGVRE IYGIPAGYRG FYSMKAVKLD PKAVHDWHKK GGTVLATSRG 150
    GFHLQKIVDA IHLNGYNQVY IIGGDGTMRG AVEIFKEISL RKLEVGITVI 200
    PKTVDNDVGI IDRSFGFQTA VEMAQEAISA AHVEAESAVN GIGLVKLMGR 250
    STGHIALHAT LSSRDVDCCL IPEMDFYLEG KGGLFEFLEK RLKERGHAVL 300
    VVAEGAGQEM IPRNESQKQE RDESGNAVFL DVGVWFKSVL KAWWEREHPD 350
    ELFTVKYIDP TYMIRAVPAN ATDNLYCTLL AHSAIHGVMA GYTGFVPGPI 400
    NGNYAYIPLE EVAQTKNQVN TRDHKWAWVR SVTNQPDFET NVKG 444
    Length:444
    Mass (Da):49,182
    Last modified:March 1, 2001 - v1
    Checksum:i42E78785792A73EC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB016886 Genomic DNA. Translation: BAB11328.1.
    CP002688 Genomic DNA. Translation: AED95574.1.
    AY056779 mRNA. Translation: AAL09725.1.
    AY090379 mRNA. Translation: AAL91281.1.
    RefSeqiNP_199592.1. NM_124155.2.
    UniGeneiAt.9400.

    Genome annotation databases

    EnsemblPlantsiAT5G47810.1; AT5G47810.1; AT5G47810.
    GeneIDi834832.
    KEGGiath:AT5G47810.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB016886 Genomic DNA. Translation: BAB11328.1 .
    CP002688 Genomic DNA. Translation: AED95574.1 .
    AY056779 mRNA. Translation: AAL09725.1 .
    AY090379 mRNA. Translation: AAL91281.1 .
    RefSeqi NP_199592.1. NM_124155.2.
    UniGenei At.9400.

    3D structure databases

    ProteinModelPortali Q9FIK0.
    SMRi Q9FIK0. Positions 64-433.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 20080. 1 interaction.
    IntActi Q9FIK0. 7 interactions.
    STRINGi 3702.AT5G47810.1-P.

    Proteomic databases

    PaxDbi Q9FIK0.
    PRIDEi Q9FIK0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G47810.1 ; AT5G47810.1 ; AT5G47810 .
    GeneIDi 834832.
    KEGGi ath:AT5G47810.

    Organism-specific databases

    TAIRi AT5G47810.

    Phylogenomic databases

    eggNOGi COG0205.
    HOGENOMi HOG000016186.
    InParanoidi Q9FIK0.
    KOi K00850.
    OMAi GRWWKRE.
    PhylomeDBi Q9FIK0.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci ARA:AT5G47810-MONOMER.

    Gene expression databases

    Genevestigatori Q9FIK0.

    Family and domain databases

    HAMAPi MF_01981. Phosphofructokinase_II_X.
    InterProi IPR022953. Phosphofructokinase.
    IPR000023. Phosphofructokinase_dom.
    IPR012004. PyroP-dep_PFruKinase_TP0108.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000534. PPi_PFK_TP0108. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones."
      Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S.
      DNA Res. 5:379-391(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Characterisation of the ATP-dependent phosphofructokinase gene family from Arabidopsis thaliana."
      Mustroph A., Sonnewald U., Biemelt S.
      FEBS Lett. 581:2401-2410(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiPFKA2_ARATH
    AccessioniPrimary (citable) accession number: Q9FIK0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3