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Protein

Respiratory burst oxidase homolog protein D

Gene

RBOHD

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-dependent NADPH oxidase that generates superoxide. Involved in the generation of reactive oxygen species (ROS) during incompatible interactions with pathogens and in UV-B and abscisic acid ROS-dependent signaling. Might be required for ROS signal amplification during light stress.6 Publications

Enzyme regulationi

Inhibited by diphenylene iodinium (DPI).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi266CalciumBy similarity1
Metal bindingi268CalciumBy similarity1
Metal bindingi270CalciumBy similarity1
Metal bindingi272Calcium; via carbonyl oxygenBy similarity1
Metal bindingi277CalciumBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi266 – 277PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • NAD(P)H oxidase activity Source: TAIR
  • peroxidase activity Source: UniProtKB-KW

GO - Biological processi

  • carbohydrate homeostasis Source: TAIR
  • defense response to fungus Source: TAIR
  • negative regulation of programmed cell death Source: TAIR
  • osmosensory signaling pathway Source: TAIR
  • reactive oxygen species metabolic process Source: TAIR
  • response to heat Source: TAIR
  • response to wounding Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Plant defense

Keywords - Ligandi

Calcium, FAD, Flavoprotein, Metal-binding, NADP

Enzyme and pathway databases

BioCyciARA:AT5G47910-MONOMER.
BRENDAi1.6.3.1. 399.
ReactomeiR-ATH-209968. Thyroxine biosynthesis.
R-ATH-3299685. Detoxification of Reactive Oxygen Species.
R-ATH-6798695. Neutrophil degranulation.

Protein family/group databases

PeroxiBasei3286. AtRboh04.

Names & Taxonomyi

Protein namesi
Recommended name:
Respiratory burst oxidase homolog protein D (EC:1.11.1.-, EC:1.6.3.-)
Alternative name(s):
NADPH oxidase RBOHD
Short name:
AtRBOHD
Gene namesi
Name:RBOHD
Ordered Locus Names:At5g47910
ORF Names:MCA23.25
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G47910.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 376CytoplasmicSequence analysisAdd BLAST376
Transmembranei377 – 397Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini398 – 461ExtracellularSequence analysisAdd BLAST64
Transmembranei462 – 482Helical; Name=2By similarityAdd BLAST21
Topological domaini483 – 516CytoplasmicSequence analysisAdd BLAST34
Transmembranei517 – 537Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini538 – 559ExtracellularSequence analysisAdd BLAST22
Transmembranei560 – 580Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini581 – 588CytoplasmicSequence analysis8
Transmembranei589 – 606Helical; Name=5By similarityAdd BLAST18
Topological domaini607 – 734ExtracellularSequence analysisAdd BLAST128
Transmembranei735 – 755Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini756 – 921CytoplasmicSequence analysisAdd BLAST166

GO - Cellular componenti

  • Golgi apparatus Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Plants do not accumulate reactive oxygen species during disease-resistance reactions, do not up-regulate UV-B-dependent gene expression and are impaired in abscisic acid-induced stomatal closing and in root growth and seed germination inhibitions.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003137561 – 921Respiratory burst oxidase homolog protein DAdd BLAST921

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei26PhosphoserineCombined sources1
Modified residuei39PhosphoserineCombined sources1 Publication1
Modified residuei343PhosphoserineCombined sources1 Publication1
Modified residuei347PhosphoserineCombined sources1 Publication1

Post-translational modificationi

Phosphorylated at Ser-39, Ser-343 and Ser-347 by BIK1 upon flagellin (flg22) treatment.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9FIJ0.
PRIDEiQ9FIJ0.

PTM databases

iPTMnetiQ9FIJ0.

Expressioni

Tissue specificityi

More abundant in roots than in leaves, stems or inflorescences. Expressed in mesophyll and guard cells.2 Publications

Inductioni

Up-regulated by pathogen infection and by abscisic acid.2 Publications

Gene expression databases

GenevisibleiQ9FIJ0. AT.

Interactioni

Subunit structurei

Monomer and homodimer (By similarity). Interacts with BIK1 and FLS2 (PubMed:24629339). Interacts with PBL13 (PubMed:26432875).By similarity2 Publications

Protein-protein interaction databases

BioGridi20090. 37 interactors.
STRINGi3702.AT5G47910.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FIJ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini253 – 288EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini297 – 332EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini415 – 572Ferric oxidoreductaseAdd BLAST158
Domaini611 – 732FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST122

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni193 – 203EF-hand-like 1By similarityAdd BLAST11
Regioni230 – 241EF-hand-like 2By similarityAdd BLAST12

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi98 – 101Poly-Gly4

Sequence similaritiesi

Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 ferric oxidoreductase domain.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
HOGENOMiHOG000216670.
InParanoidiQ9FIJ0.
KOiK13447.
OMAiDARWLEW.
OrthoDBiEOG093601IF.
PhylomeDBiQ9FIJ0.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR000778. Cyt_b245_heavy_chain.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR013623. NADPH_Ox.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
PF08414. NADPH_Ox. 1 hit.
[Graphical view]
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FIJ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMRRGNSSN DHELGILRGA NSDTNSDTES IASDRGAFSG PLGRPKRASK
60 70 80 90 100
KNARFADDLP KRSNSVAGGR GDDDEYVEIT LDIRDDSVAV HSVQQAAGGG
110 120 130 140 150
GHLEDPELAL LTKKTLESSL NNTTSLSFFR STSSRIKNAS RELRRVFSRR
160 170 180 190 200
PSPAVRRFDR TSSAAIHALK GLKFIATKTA AWPAVDQRFD KLSADSNGLL
210 220 230 240 250
LSAKFWECLG MNKESKDFAD QLFRALARRN NVSGDAITKE QLRIFWEQIS
260 270 280 290 300
DESFDAKLQV FFDMVDKDED GRVTEEEVAE IISLSASANK LSNIQKQAKE
310 320 330 340 350
YAALIMEELD PDNAGFIMIE NLEMLLLQAP NQSVRMGDSR ILSQMLSQKL
360 370 380 390 400
RPAKESNPLV RWSEKIKYFI LDNWQRLWIM MLWLGICGGL FTYKFIQYKN
410 420 430 440 450
KAAYGVMGYC VCVAKGGAET LKFNMALILL PVCRNTITWL RNKTKLGTVV
460 470 480 490 500
PFDDSLNFHK VIASGIVVGV LLHAGAHLTC DFPRLIAADE DTYEPMEKYF
510 520 530 540 550
GDQPTSYWWF VKGVEGWTGI VMVVLMAIAF TLATPWFRRN KLNLPNFLKK
560 570 580 590 600
LTGFNAFWYT HHLFIIVYAL LIVHGIKLYL TKIWYQKTTW MYLAVPILLY
610 620 630 640 650
ASERLLRAFR SSIKPVKMIK VAVYPGNVLS LHMTKPQGFK YKSGQFMLVN
660 670 680 690 700
CRAVSPFEWH PFSITSAPGD DYLSVHIRTL GDWTRKLRTV FSEVCKPPTA
710 720 730 740 750
GKSGLLRADG GDGNLPFPKV LIDGPYGAPA QDYKKYDVVL LVGLGIGATP
760 770 780 790 800
MISILKDIIN NMKGPDRDSD IENNNSNNNS KGFKTRKAYF YWVTREQGSF
810 820 830 840 850
EWFKGIMDEI SELDEEGIIE LHNYCTSVYE EGDARVALIA MLQSLQHAKN
860 870 880 890 900
GVDVVSGTRV KSHFAKPNWR QVYKKIAVQH PGKRIGVFYC GMPGMIKELK
910 920
NLALDFSRKT TTKFDFHKEN F
Length:921
Mass (Da):103,909
Last modified:March 1, 2001 - v1
Checksum:i1A822569A7AB1817
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055357 mRNA. Translation: AAC39479.1.
AB016886 Genomic DNA. Translation: BAB11338.1.
CP002688 Genomic DNA. Translation: AED95593.1.
AF424625 mRNA. Translation: AAL11618.1.
BT002651 mRNA. Translation: AAO11567.1.
PIRiT51804.
RefSeqiNP_199602.1. NM_124165.3.
UniGeneiAt.23270.

Genome annotation databases

EnsemblPlantsiAT5G47910.1; AT5G47910.1; AT5G47910.
GeneIDi834842.
GrameneiAT5G47910.1; AT5G47910.1; AT5G47910.
KEGGiath:AT5G47910.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055357 mRNA. Translation: AAC39479.1.
AB016886 Genomic DNA. Translation: BAB11338.1.
CP002688 Genomic DNA. Translation: AED95593.1.
AF424625 mRNA. Translation: AAL11618.1.
BT002651 mRNA. Translation: AAO11567.1.
PIRiT51804.
RefSeqiNP_199602.1. NM_124165.3.
UniGeneiAt.23270.

3D structure databases

ProteinModelPortaliQ9FIJ0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi20090. 37 interactors.
STRINGi3702.AT5G47910.1.

Protein family/group databases

PeroxiBasei3286. AtRboh04.

PTM databases

iPTMnetiQ9FIJ0.

Proteomic databases

PaxDbiQ9FIJ0.
PRIDEiQ9FIJ0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G47910.1; AT5G47910.1; AT5G47910.
GeneIDi834842.
GrameneiAT5G47910.1; AT5G47910.1; AT5G47910.
KEGGiath:AT5G47910.

Organism-specific databases

TAIRiAT5G47910.

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
HOGENOMiHOG000216670.
InParanoidiQ9FIJ0.
KOiK13447.
OMAiDARWLEW.
OrthoDBiEOG093601IF.
PhylomeDBiQ9FIJ0.

Enzyme and pathway databases

BioCyciARA:AT5G47910-MONOMER.
BRENDAi1.6.3.1. 399.
ReactomeiR-ATH-209968. Thyroxine biosynthesis.
R-ATH-3299685. Detoxification of Reactive Oxygen Species.
R-ATH-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ9FIJ0.

Gene expression databases

GenevisibleiQ9FIJ0. AT.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR000778. Cyt_b245_heavy_chain.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR013623. NADPH_Ox.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
PF08414. NADPH_Ox. 1 hit.
[Graphical view]
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRBOHD_ARATH
AccessioniPrimary (citable) accession number: Q9FIJ0
Secondary accession number(s): O81212
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.