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Q9FIJ0

- RBOHD_ARATH

UniProt

Q9FIJ0 - RBOHD_ARATH

Protein

Respiratory burst oxidase homolog protein D

Gene

RBOHD

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Calcium-dependent NADPH oxidase that generates superoxide. Involved in the generation of reactive oxygen species (ROS) during incompatible interactions with pathogens and in UV-B and abscisic acid ROS-dependent signaling. Might be required for ROS signal amplification during light stress.6 Publications

    Enzyme regulationi

    Inhibited by diphenylene iodinium (DPI).1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi266 – 2661CalciumBy similarity
    Metal bindingi268 – 2681CalciumBy similarity
    Metal bindingi270 – 2701CalciumBy similarity
    Metal bindingi272 – 2721Calcium; via carbonyl oxygenBy similarity
    Metal bindingi277 – 2771CalciumBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi266 – 27712PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. NAD(P)H oxidase activity Source: TAIR
    3. peroxidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. carbohydrate homeostasis Source: TAIR
    2. defense response to fungus Source: TAIR
    3. negative regulation of programmed cell death Source: TAIR
    4. osmosensory signaling pathway Source: TAIR
    5. reactive oxygen species metabolic process Source: TAIR
    6. response to heat Source: TAIR
    7. response to wounding Source: TAIR

    Keywords - Molecular functioni

    Oxidoreductase, Peroxidase

    Keywords - Ligandi

    Calcium, FAD, Flavoprotein, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciARA:AT5G47910-MONOMER.

    Protein family/group databases

    PeroxiBasei3286. AtRboh04.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Respiratory burst oxidase homolog protein D (EC:1.11.1.-, EC:1.6.3.-)
    Alternative name(s):
    NADPH oxidase RBOHD
    Short name:
    AtRBOHD
    Gene namesi
    Name:RBOHD
    Ordered Locus Names:At5g47910
    ORF Names:MCA23.25
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G47910.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi apparatus Source: TAIR
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: TAIR

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Plants do not accumulate reactive oxygen species during disease-resistance reactions, do not up-regulate UV-B-dependent gene expression and are impaired in abscisic acid-induced stomatal closing and in root growth and seed germination inhibitions.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 921921Respiratory burst oxidase homolog protein DPRO_0000313756Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei26 – 261Phosphoserine1 Publication
    Modified residuei39 – 391Phosphoserine2 Publications
    Modified residuei343 – 3431Phosphoserine1 Publication
    Modified residuei347 – 3471Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9FIJ0.
    PRIDEiQ9FIJ0.

    Expressioni

    Tissue specificityi

    More abundant in roots than in leaves, stems or inflorescences. Expressed in mesophyll and guard cells.2 Publications

    Inductioni

    Up-regulated by pathogen infection and by abscisic acid.2 Publications

    Gene expression databases

    GenevestigatoriQ9FIJ0.

    Interactioni

    Subunit structurei

    Monomer and homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FIJ0.
    SMRiQ9FIJ0. Positions 161-328, 720-921.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 376376CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini398 – 46164ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini483 – 51634CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini538 – 55922ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini581 – 5888CytoplasmicSequence Analysis
    Topological domaini607 – 734128ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini756 – 921166CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei377 – 39721Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei462 – 48221Helical; Name=2By similarityAdd
    BLAST
    Transmembranei517 – 53721Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei560 – 58021Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei589 – 60618Helical; Name=5By similarityAdd
    BLAST
    Transmembranei735 – 75521Helical; Name=6Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini253 – 28836EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini297 – 33236EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini415 – 572158Ferric oxidoreductaseAdd
    BLAST
    Domaini611 – 732122FAD-binding FR-typePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni193 – 20311EF-hand-like 1By similarityAdd
    BLAST
    Regioni230 – 24112EF-hand-like 2By similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi98 – 1014Poly-Gly

    Sequence similaritiesi

    Contains 2 EF-hand domains.PROSITE-ProRule annotation
    Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
    Contains 1 ferric oxidoreductase domain.Curated

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG4097.
    HOGENOMiHOG000216670.
    InParanoidiQ9FIJ0.
    KOiK13447.
    OMAiFTYKFIQ.
    PhylomeDBiQ9FIJ0.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR000778. Cyt_b245_heavy_chain.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR013112. FAD-bd_8.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR013130. Fe3_Rdtase_TM_dom.
    IPR013121. Fe_red_NAD-bd_6.
    IPR013623. NADPH_Ox.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF08022. FAD_binding_8. 1 hit.
    PF01794. Ferric_reduct. 1 hit.
    PF08030. NAD_binding_6. 1 hit.
    PF08414. NADPH_Ox. 1 hit.
    [Graphical view]
    PRINTSiPR00466. GP91PHOX.
    SUPFAMiSSF63380. SSF63380. 1 hit.
    PROSITEiPS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 2 hits.
    PS51384. FAD_FR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9FIJ0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKMRRGNSSN DHELGILRGA NSDTNSDTES IASDRGAFSG PLGRPKRASK    50
    KNARFADDLP KRSNSVAGGR GDDDEYVEIT LDIRDDSVAV HSVQQAAGGG 100
    GHLEDPELAL LTKKTLESSL NNTTSLSFFR STSSRIKNAS RELRRVFSRR 150
    PSPAVRRFDR TSSAAIHALK GLKFIATKTA AWPAVDQRFD KLSADSNGLL 200
    LSAKFWECLG MNKESKDFAD QLFRALARRN NVSGDAITKE QLRIFWEQIS 250
    DESFDAKLQV FFDMVDKDED GRVTEEEVAE IISLSASANK LSNIQKQAKE 300
    YAALIMEELD PDNAGFIMIE NLEMLLLQAP NQSVRMGDSR ILSQMLSQKL 350
    RPAKESNPLV RWSEKIKYFI LDNWQRLWIM MLWLGICGGL FTYKFIQYKN 400
    KAAYGVMGYC VCVAKGGAET LKFNMALILL PVCRNTITWL RNKTKLGTVV 450
    PFDDSLNFHK VIASGIVVGV LLHAGAHLTC DFPRLIAADE DTYEPMEKYF 500
    GDQPTSYWWF VKGVEGWTGI VMVVLMAIAF TLATPWFRRN KLNLPNFLKK 550
    LTGFNAFWYT HHLFIIVYAL LIVHGIKLYL TKIWYQKTTW MYLAVPILLY 600
    ASERLLRAFR SSIKPVKMIK VAVYPGNVLS LHMTKPQGFK YKSGQFMLVN 650
    CRAVSPFEWH PFSITSAPGD DYLSVHIRTL GDWTRKLRTV FSEVCKPPTA 700
    GKSGLLRADG GDGNLPFPKV LIDGPYGAPA QDYKKYDVVL LVGLGIGATP 750
    MISILKDIIN NMKGPDRDSD IENNNSNNNS KGFKTRKAYF YWVTREQGSF 800
    EWFKGIMDEI SELDEEGIIE LHNYCTSVYE EGDARVALIA MLQSLQHAKN 850
    GVDVVSGTRV KSHFAKPNWR QVYKKIAVQH PGKRIGVFYC GMPGMIKELK 900
    NLALDFSRKT TTKFDFHKEN F 921
    Length:921
    Mass (Da):103,909
    Last modified:March 1, 2001 - v1
    Checksum:i1A822569A7AB1817
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF055357 mRNA. Translation: AAC39479.1.
    AB016886 Genomic DNA. Translation: BAB11338.1.
    CP002688 Genomic DNA. Translation: AED95593.1.
    AF424625 mRNA. Translation: AAL11618.1.
    BT002651 mRNA. Translation: AAO11567.1.
    PIRiT51804.
    RefSeqiNP_199602.1. NM_124165.2.
    UniGeneiAt.23270.

    Genome annotation databases

    EnsemblPlantsiAT5G47910.1; AT5G47910.1; AT5G47910.
    GeneIDi834842.
    KEGGiath:AT5G47910.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF055357 mRNA. Translation: AAC39479.1 .
    AB016886 Genomic DNA. Translation: BAB11338.1 .
    CP002688 Genomic DNA. Translation: AED95593.1 .
    AF424625 mRNA. Translation: AAL11618.1 .
    BT002651 mRNA. Translation: AAO11567.1 .
    PIRi T51804.
    RefSeqi NP_199602.1. NM_124165.2.
    UniGenei At.23270.

    3D structure databases

    ProteinModelPortali Q9FIJ0.
    SMRi Q9FIJ0. Positions 161-328, 720-921.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    PeroxiBasei 3286. AtRboh04.

    Proteomic databases

    PaxDbi Q9FIJ0.
    PRIDEi Q9FIJ0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G47910.1 ; AT5G47910.1 ; AT5G47910 .
    GeneIDi 834842.
    KEGGi ath:AT5G47910.

    Organism-specific databases

    TAIRi AT5G47910.

    Phylogenomic databases

    eggNOGi COG4097.
    HOGENOMi HOG000216670.
    InParanoidi Q9FIJ0.
    KOi K13447.
    OMAi FTYKFIQ.
    PhylomeDBi Q9FIJ0.

    Enzyme and pathway databases

    BioCyci ARA:AT5G47910-MONOMER.

    Gene expression databases

    Genevestigatori Q9FIJ0.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR000778. Cyt_b245_heavy_chain.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR013112. FAD-bd_8.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR013130. Fe3_Rdtase_TM_dom.
    IPR013121. Fe_red_NAD-bd_6.
    IPR013623. NADPH_Ox.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view ]
    Pfami PF08022. FAD_binding_8. 1 hit.
    PF01794. Ferric_reduct. 1 hit.
    PF08030. NAD_binding_6. 1 hit.
    PF08414. NADPH_Ox. 1 hit.
    [Graphical view ]
    PRINTSi PR00466. GP91PHOX.
    SUPFAMi SSF63380. SSF63380. 1 hit.
    PROSITEi PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 2 hits.
    PS51384. FAD_FR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Six Arabidopsis thaliana homologues of the human respiratory burst oxidase (gp91phox)."
      Torres M.A., Onouchi H., Hamada S., Machida C., Hammond-Kosack K.E., Jones J.D.G.
      Plant J. 14:365-370(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: cv. Landsberg erecta.
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones."
      Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S.
      DNA Res. 5:379-391(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Arabidopsis gp91phox homologues AtrbohD and AtrbohF are required for accumulation of reactive oxygen intermediates in the plant defense response."
      Torres M.A., Dangl J.L., Jones J.D.G.
      Proc. Natl. Acad. Sci. U.S.A. 99:517-522(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "NADPH oxidase AtrbohD and AtrbohF genes function in ROS-dependent ABA signaling in Arabidopsis."
      Kwak J.M., Mori I.C., Pei Z.-M., Leonhardt N., Torres M.A., Dangl J.L., Bloom R.E., Bodde S., Jones J.D.G., Schroeder J.I.
      EMBO J. 22:2623-2633(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION BY ABSCISIC ACID, TISSUE SPECIFICITY.
    7. "Cytosolic ascorbate peroxidase 1 is a central component of the reactive oxygen gene network of Arabidopsis."
      Davletova S., Rizhsky L., Liang H., Shengqiang Z., Oliver D.J., Coutu J., Shulaev V., Schlauch K., Mittler R.
      Plant Cell 17:268-281(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    8. "The role of NADPH oxidase and MAP kinase phosphatase in UV-B-dependent gene expression in Arabidopsis."
      Kalbina I., Strid A.
      Plant Cell Environ. 29:1783-1793(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Extracellular ATP induces the accumulation of superoxide via NADPH oxidases in Arabidopsis."
      Song C.J., Steinebrunner I., Wang X., Stout S.C., Roux S.J.
      Plant Physiol. 140:1222-1232(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "Production of reactive oxygen species by plant NADPH oxidases."
      Sagi M., Fluhr R.
      Plant Physiol. 141:336-340(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    11. "Arabidopsis thaliana expresses multiple lines of defense to counterattack Erwinia chrysanthemi."
      Fagard M., Dellagi A., Roux C., Perino C., Rigault M., Boucher V., Shevchik V.E., Expert D.
      Mol. Plant Microbe Interact. 20:794-805(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ENZYME REGULATION, INDUCTION.
    12. "Quantitative phosphoproteomic analysis of plasma membrane proteins reveals regulatory mechanisms of plant innate immune responses."
      Nuehse T.S., Bottrill A.R., Jones A.M., Peck S.C.
      Plant J. 51:931-940(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26; SER-343 AND SER-347, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: cv. La-0.
    13. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
      Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
      J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: cv. Columbia.
    14. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
      Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
      Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRBOHD_ARATH
    AccessioniPrimary (citable) accession number: Q9FIJ0
    Secondary accession number(s): O81212
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3