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Q9FIJ0

- RBOHD_ARATH

UniProt

Q9FIJ0 - RBOHD_ARATH

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Protein

Respiratory burst oxidase homolog protein D

Gene

RBOHD

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Calcium-dependent NADPH oxidase that generates superoxide. Involved in the generation of reactive oxygen species (ROS) during incompatible interactions with pathogens and in UV-B and abscisic acid ROS-dependent signaling. Might be required for ROS signal amplification during light stress.6 Publications

Enzyme regulationi

Inhibited by diphenylene iodinium (DPI).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi266 – 2661CalciumBy similarity
Metal bindingi268 – 2681CalciumBy similarity
Metal bindingi270 – 2701CalciumBy similarity
Metal bindingi272 – 2721Calcium; via carbonyl oxygenBy similarity
Metal bindingi277 – 2771CalciumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi266 – 27712PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. NAD(P)H oxidase activity Source: TAIR
  3. peroxidase activity Source: UniProtKB-KW

GO - Biological processi

  1. carbohydrate homeostasis Source: TAIR
  2. defense response to fungus Source: TAIR
  3. negative regulation of programmed cell death Source: TAIR
  4. osmosensory signaling pathway Source: TAIR
  5. reactive oxygen species metabolic process Source: TAIR
  6. response to heat Source: TAIR
  7. response to wounding Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Ligandi

Calcium, FAD, Flavoprotein, Metal-binding, NADP

Enzyme and pathway databases

BioCyciARA:AT5G47910-MONOMER.

Protein family/group databases

PeroxiBasei3286. AtRboh04.

Names & Taxonomyi

Protein namesi
Recommended name:
Respiratory burst oxidase homolog protein D (EC:1.11.1.-, EC:1.6.3.-)
Alternative name(s):
NADPH oxidase RBOHD
Short name:
AtRBOHD
Gene namesi
Name:RBOHD
Ordered Locus Names:At5g47910
ORF Names:MCA23.25
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G47910.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 376376CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei377 – 39721Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini398 – 46164ExtracellularSequence AnalysisAdd
BLAST
Transmembranei462 – 48221Helical; Name=2By similarityAdd
BLAST
Topological domaini483 – 51634CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei517 – 53721Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini538 – 55922ExtracellularSequence AnalysisAdd
BLAST
Transmembranei560 – 58021Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini581 – 5888CytoplasmicSequence Analysis
Transmembranei589 – 60618Helical; Name=5By similarityAdd
BLAST
Topological domaini607 – 734128ExtracellularSequence AnalysisAdd
BLAST
Transmembranei735 – 75521Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini756 – 921166CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: TAIR
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Plants do not accumulate reactive oxygen species during disease-resistance reactions, do not up-regulate UV-B-dependent gene expression and are impaired in abscisic acid-induced stomatal closing and in root growth and seed germination inhibitions.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 921921Respiratory burst oxidase homolog protein DPRO_0000313756Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei26 – 261Phosphoserine1 Publication
Modified residuei39 – 391Phosphoserine2 Publications
Modified residuei343 – 3431Phosphoserine1 Publication
Modified residuei347 – 3471Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9FIJ0.
PRIDEiQ9FIJ0.

Expressioni

Tissue specificityi

More abundant in roots than in leaves, stems or inflorescences. Expressed in mesophyll and guard cells.2 Publications

Inductioni

Up-regulated by pathogen infection and by abscisic acid.2 Publications

Gene expression databases

GenevestigatoriQ9FIJ0.

Interactioni

Subunit structurei

Monomer and homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9FIJ0.
SMRiQ9FIJ0. Positions 161-328, 720-921.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini253 – 28836EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini297 – 33236EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini415 – 572158Ferric oxidoreductaseAdd
BLAST
Domaini611 – 732122FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni193 – 20311EF-hand-like 1By similarityAdd
BLAST
Regioni230 – 24112EF-hand-like 2By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi98 – 1014Poly-Gly

Sequence similaritiesi

Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 ferric oxidoreductase domain.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG4097.
HOGENOMiHOG000216670.
InParanoidiQ9FIJ0.
KOiK13447.
OMAiFTYKFIQ.
PhylomeDBiQ9FIJ0.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR000778. Cyt_b245_heavy_chain.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR013623. NADPH_Ox.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
PF08414. NADPH_Ox. 1 hit.
[Graphical view]
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FIJ0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKMRRGNSSN DHELGILRGA NSDTNSDTES IASDRGAFSG PLGRPKRASK
60 70 80 90 100
KNARFADDLP KRSNSVAGGR GDDDEYVEIT LDIRDDSVAV HSVQQAAGGG
110 120 130 140 150
GHLEDPELAL LTKKTLESSL NNTTSLSFFR STSSRIKNAS RELRRVFSRR
160 170 180 190 200
PSPAVRRFDR TSSAAIHALK GLKFIATKTA AWPAVDQRFD KLSADSNGLL
210 220 230 240 250
LSAKFWECLG MNKESKDFAD QLFRALARRN NVSGDAITKE QLRIFWEQIS
260 270 280 290 300
DESFDAKLQV FFDMVDKDED GRVTEEEVAE IISLSASANK LSNIQKQAKE
310 320 330 340 350
YAALIMEELD PDNAGFIMIE NLEMLLLQAP NQSVRMGDSR ILSQMLSQKL
360 370 380 390 400
RPAKESNPLV RWSEKIKYFI LDNWQRLWIM MLWLGICGGL FTYKFIQYKN
410 420 430 440 450
KAAYGVMGYC VCVAKGGAET LKFNMALILL PVCRNTITWL RNKTKLGTVV
460 470 480 490 500
PFDDSLNFHK VIASGIVVGV LLHAGAHLTC DFPRLIAADE DTYEPMEKYF
510 520 530 540 550
GDQPTSYWWF VKGVEGWTGI VMVVLMAIAF TLATPWFRRN KLNLPNFLKK
560 570 580 590 600
LTGFNAFWYT HHLFIIVYAL LIVHGIKLYL TKIWYQKTTW MYLAVPILLY
610 620 630 640 650
ASERLLRAFR SSIKPVKMIK VAVYPGNVLS LHMTKPQGFK YKSGQFMLVN
660 670 680 690 700
CRAVSPFEWH PFSITSAPGD DYLSVHIRTL GDWTRKLRTV FSEVCKPPTA
710 720 730 740 750
GKSGLLRADG GDGNLPFPKV LIDGPYGAPA QDYKKYDVVL LVGLGIGATP
760 770 780 790 800
MISILKDIIN NMKGPDRDSD IENNNSNNNS KGFKTRKAYF YWVTREQGSF
810 820 830 840 850
EWFKGIMDEI SELDEEGIIE LHNYCTSVYE EGDARVALIA MLQSLQHAKN
860 870 880 890 900
GVDVVSGTRV KSHFAKPNWR QVYKKIAVQH PGKRIGVFYC GMPGMIKELK
910 920
NLALDFSRKT TTKFDFHKEN F
Length:921
Mass (Da):103,909
Last modified:March 1, 2001 - v1
Checksum:i1A822569A7AB1817
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055357 mRNA. Translation: AAC39479.1.
AB016886 Genomic DNA. Translation: BAB11338.1.
CP002688 Genomic DNA. Translation: AED95593.1.
AF424625 mRNA. Translation: AAL11618.1.
BT002651 mRNA. Translation: AAO11567.1.
PIRiT51804.
RefSeqiNP_199602.1. NM_124165.2.
UniGeneiAt.23270.

Genome annotation databases

EnsemblPlantsiAT5G47910.1; AT5G47910.1; AT5G47910.
GeneIDi834842.
KEGGiath:AT5G47910.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055357 mRNA. Translation: AAC39479.1 .
AB016886 Genomic DNA. Translation: BAB11338.1 .
CP002688 Genomic DNA. Translation: AED95593.1 .
AF424625 mRNA. Translation: AAL11618.1 .
BT002651 mRNA. Translation: AAO11567.1 .
PIRi T51804.
RefSeqi NP_199602.1. NM_124165.2.
UniGenei At.23270.

3D structure databases

ProteinModelPortali Q9FIJ0.
SMRi Q9FIJ0. Positions 161-328, 720-921.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

PeroxiBasei 3286. AtRboh04.

Proteomic databases

PaxDbi Q9FIJ0.
PRIDEi Q9FIJ0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G47910.1 ; AT5G47910.1 ; AT5G47910 .
GeneIDi 834842.
KEGGi ath:AT5G47910.

Organism-specific databases

TAIRi AT5G47910.

Phylogenomic databases

eggNOGi COG4097.
HOGENOMi HOG000216670.
InParanoidi Q9FIJ0.
KOi K13447.
OMAi FTYKFIQ.
PhylomeDBi Q9FIJ0.

Enzyme and pathway databases

BioCyci ARA:AT5G47910-MONOMER.

Gene expression databases

Genevestigatori Q9FIJ0.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR000778. Cyt_b245_heavy_chain.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR013623. NADPH_Ox.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
PF08414. NADPH_Ox. 1 hit.
[Graphical view ]
PRINTSi PR00466. GP91PHOX.
SUPFAMi SSF63380. SSF63380. 1 hit.
PROSITEi PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS51384. FAD_FR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Six Arabidopsis thaliana homologues of the human respiratory burst oxidase (gp91phox)."
    Torres M.A., Onouchi H., Hamada S., Machida C., Hammond-Kosack K.E., Jones J.D.G.
    Plant J. 14:365-370(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: cv. Landsberg erecta.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones."
    Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S.
    DNA Res. 5:379-391(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Arabidopsis gp91phox homologues AtrbohD and AtrbohF are required for accumulation of reactive oxygen intermediates in the plant defense response."
    Torres M.A., Dangl J.L., Jones J.D.G.
    Proc. Natl. Acad. Sci. U.S.A. 99:517-522(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "NADPH oxidase AtrbohD and AtrbohF genes function in ROS-dependent ABA signaling in Arabidopsis."
    Kwak J.M., Mori I.C., Pei Z.-M., Leonhardt N., Torres M.A., Dangl J.L., Bloom R.E., Bodde S., Jones J.D.G., Schroeder J.I.
    EMBO J. 22:2623-2633(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION BY ABSCISIC ACID, TISSUE SPECIFICITY.
  7. "Cytosolic ascorbate peroxidase 1 is a central component of the reactive oxygen gene network of Arabidopsis."
    Davletova S., Rizhsky L., Liang H., Shengqiang Z., Oliver D.J., Coutu J., Shulaev V., Schlauch K., Mittler R.
    Plant Cell 17:268-281(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  8. "The role of NADPH oxidase and MAP kinase phosphatase in UV-B-dependent gene expression in Arabidopsis."
    Kalbina I., Strid A.
    Plant Cell Environ. 29:1783-1793(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Extracellular ATP induces the accumulation of superoxide via NADPH oxidases in Arabidopsis."
    Song C.J., Steinebrunner I., Wang X., Stout S.C., Roux S.J.
    Plant Physiol. 140:1222-1232(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Production of reactive oxygen species by plant NADPH oxidases."
    Sagi M., Fluhr R.
    Plant Physiol. 141:336-340(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  11. "Arabidopsis thaliana expresses multiple lines of defense to counterattack Erwinia chrysanthemi."
    Fagard M., Dellagi A., Roux C., Perino C., Rigault M., Boucher V., Shevchik V.E., Expert D.
    Mol. Plant Microbe Interact. 20:794-805(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION, INDUCTION.
  12. "Quantitative phosphoproteomic analysis of plasma membrane proteins reveals regulatory mechanisms of plant innate immune responses."
    Nuehse T.S., Bottrill A.R., Jones A.M., Peck S.C.
    Plant J. 51:931-940(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26; SER-343 AND SER-347, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: cv. La-0.
  13. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
    Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
    J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.
  14. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRBOHD_ARATH
AccessioniPrimary (citable) accession number: Q9FIJ0
Secondary accession number(s): O81212
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2001
Last modified: October 1, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3