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Protein

Probable protein phosphatase 2C 78

Gene

SAG113

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Acts as negative regulator of abscisic acid (ABA) signaling for stomatal closure in leaves, and controls water loss during leaf senescence (PubMed:22007837, PubMed:22184656). Activated by the NAC029/NAP transcription factor during ABA signaling in senescing leaves (PubMed:22184656). Functions as negative regulator of osmotic stress and ABA signaling (PubMed:22198272). Acts as negative regulator of response to drought (PubMed:22829320).4 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.PROSITE-ProRule annotation

Cofactori

Mg2+PROSITE-ProRule annotation, Mn2+PROSITE-ProRule annotationNote: Binds 2 magnesium or manganese ions per subunit.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi153Manganese 1By similarity1
Metal bindingi153Manganese 2By similarity1
Metal bindingi154Manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi327Manganese 2By similarity1
Metal bindingi400Manganese 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: TAIR

GO - Biological processi

  • chloroplast organization Source: TAIR
  • leaf senescence Source: TAIR
  • negative regulation of abscisic acid-activated signaling pathway Source: TAIR
  • response to abscisic acid Source: TAIR
  • response to water deprivation Source: TAIR
  • stomatal movement Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.3.16. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protein phosphatase 2C 78 (EC:3.1.3.16PROSITE-ProRule annotation)
Short name:
AtPP2C78
Alternative name(s):
Protein HIGHLY ABA-INDUCED 11 Publication
Protein SENESCENCE-ASSOCIATED GENE 1131 Publication
Gene namesi
Name:SAG1131 Publication
Synonyms:HAI11 Publication
Ordered Locus Names:At5g59220
ORF Names:MNC17.110
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G59220.

Subcellular locationi

GO - Cellular componenti

  • cis-Golgi network membrane Source: TAIR
  • cytosol Source: TAIR
  • Golgi apparatus Source: TAIR
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Nucleus

Pathology & Biotechi

Disruption phenotypei

Delayed senescence. Increased sensitivity to abscisic acid (ABA).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003679971 – 413Probable protein phosphatase 2C 78Add BLAST413

Proteomic databases

PaxDbiQ9FIF5.

Expressioni

Inductioni

By abscisic acid (ABA) (PubMed:22007837, PubMed:22198272). Induced during leaf senescence (PubMed:22007837). Induced by osmotic stress (PubMed:22198272).2 Publications

Gene expression databases

GenevisibleiQ9FIF5. AT.

Interactioni

Protein-protein interaction databases

BioGridi21284. 35 interactors.
DIPiDIP-48992N.
IntActiQ9FIF5. 2 interactors.
STRINGi3702.AT5G59220.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FIF5.
SMRiQ9FIF5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini111 – 409PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST299

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi66 – 70Poly-Arg5

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
HOGENOMiHOG000233896.
InParanoidiQ9FIF5.
KOiK14497.
OMAiCYENETM.
OrthoDBiEOG09360IAR.
PhylomeDBiQ9FIF5.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 2 hits.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 2 hits.
PROSITEiPS51746. PPM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FIF5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEICYENET MMIETTATVV KKATTTTRRR ERSSSQAARR RRMEIRRFKF
60 70 80 90 100
VSGEQEPVFV DGDLQRRRRR ESTVAASTST VFYETAKEVV VLCESLSSTV
110 120 130 140 150
VALPDPEAYP KYGVASVCGR RREMEDAVAV HPFFSRHQTE YSSTGFHYCG
160 170 180 190 200
VYDGHGCSHV AMKCRERLHE LVREEFEADA DWEKSMARSF TRMDMEVVAL
210 220 230 240 250
NADGAAKCRC ELQRPDCDAV GSTAVVSVLT PEKIIVANCG DSRAVLCRNG
260 270 280 290 300
KAIALSSDHK PDRPDELDRI QAAGGRVIYW DGPRVLGVLA MSRAIGDNYL
310 320 330 340 350
KPYVISRPEV TVTDRANGDD FLILASDGLW DVVSNETACS VVRMCLRGKV
360 370 380 390 400
NGQVSSSPER EMTGVGAGNV VVGGGDLPDK ACEEASLLLT RLALARQSSD
410
NVSVVVVDLR RDT
Length:413
Mass (Da):45,538
Last modified:March 1, 2001 - v1
Checksum:i649CF986D17A92D7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti103L → F in AAL16163 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016890 Genomic DNA. Translation: BAB09767.1.
CP002688 Genomic DNA. Translation: AED97159.1.
AF428395 mRNA. Translation: AAL16163.1.
AY054163 mRNA. Translation: AAL06824.1.
AY074555 mRNA. Translation: AAL67095.1.
RefSeqiNP_200730.1. NM_125312.3.
UniGeneiAt.22149.

Genome annotation databases

EnsemblPlantsiAT5G59220.1; AT5G59220.1; AT5G59220.
GeneIDi836040.
GrameneiAT5G59220.1; AT5G59220.1; AT5G59220.
KEGGiath:AT5G59220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016890 Genomic DNA. Translation: BAB09767.1.
CP002688 Genomic DNA. Translation: AED97159.1.
AF428395 mRNA. Translation: AAL16163.1.
AY054163 mRNA. Translation: AAL06824.1.
AY074555 mRNA. Translation: AAL67095.1.
RefSeqiNP_200730.1. NM_125312.3.
UniGeneiAt.22149.

3D structure databases

ProteinModelPortaliQ9FIF5.
SMRiQ9FIF5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21284. 35 interactors.
DIPiDIP-48992N.
IntActiQ9FIF5. 2 interactors.
STRINGi3702.AT5G59220.1.

Proteomic databases

PaxDbiQ9FIF5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G59220.1; AT5G59220.1; AT5G59220.
GeneIDi836040.
GrameneiAT5G59220.1; AT5G59220.1; AT5G59220.
KEGGiath:AT5G59220.

Organism-specific databases

TAIRiAT5G59220.

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
HOGENOMiHOG000233896.
InParanoidiQ9FIF5.
KOiK14497.
OMAiCYENETM.
OrthoDBiEOG09360IAR.
PhylomeDBiQ9FIF5.

Enzyme and pathway databases

BRENDAi3.1.3.16. 399.

Miscellaneous databases

PROiQ9FIF5.

Gene expression databases

GenevisibleiQ9FIF5. AT.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 2 hits.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 2 hits.
PROSITEiPS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiP2C78_ARATH
AccessioniPrimary (citable) accession number: Q9FIF5
Secondary accession number(s): Q944I7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.