Q9FIE8 (UXS3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-glucuronic acid decarboxylase 3
EC=4.1.1.35

Alternative name(s):
UDP-XYL synthase 3
UDP-glucuronate decarboxylase 3
Short name=UGD
Short name=UXS-3

Gene names

Name:	UXS3
Ordered Locus Names:	At5g59290
ORF Names:	MNC17.20

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the NAD-dependent decarboxylation of UDP-glucuronic acid to UDP-xylose. Necessary for the biosynthesis of the core tetrasaccharide in glycosaminoglycan biosynthesis. Ref.1 Ref.7
Catalytic activity	UDP-D-glucuronate = UDP-D-xylose + CO2.
Cofactor	NAD. Ref.1
Pathway	Nucleotide-sugar biosynthesis; UDP-alpha-D-xylose biosynthesis; UDP-alpha-D-xylose from UDP-alpha-D-glucuronate: step 1/1.
Subcellular location	Cytoplasm Ref.6.
Tissue specificity	Ubiquitous. Ref.1
Sequence similarities	Belongs to the sugar epimerase family. UDP-glucuronic acid decarboxylase subfamily.
Biophysicochemical properties	Kinetic parameters: KM=0.51 mM for UDP-D-glucuronate at 30 degrees Celsius Ref.1 pH dependence: Optimum pH is 5.5 at 30 degrees Celsius. Temperature dependence: Optimum temperature is 30 degrees Celsius.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	NAD
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	UDP-D-xylose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	UDP-glucuronate decarboxylase activity Inferred from electronic annotation. Source: EC coenzyme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9FIE8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	UDP-glucuronic acid decarboxylase 3		PRO_0000421984
Regions
	Nucleotide binding	61 – 86	26	NAD By similarity
	Nucleotide binding	173 – 177	5	NAD By similarity
	Nucleotide binding	215 – 219	5	Substrate By similarity
	Nucleotide binding	232 – 239	8	Substrate By similarity
	Nucleotide binding	299 – 303	5	Substrate By similarity
Sites
	Active site	173	1	Proton acceptor By similarity
	Binding site	170	1	Substrate By similarity
	Binding site	202	1	Substrate By similarity
	Binding site	214	1	NAD By similarity
Experimental info
	Sequence conflict	46	1	V → D in AAK53026. Ref.4
	Sequence conflict	46	1	V → D in AAM16219. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 5E2AA6C9A13CEBD8	FASTA	342	38,569
	10 20 30 40 50 60 MAATSEKQNT TKPPPSPSPL RNSKFCQPNM RILISGGAGF IGSHLVDKLM ENEKNEVVVA 70 80 90 100 110 120 DNYFTGSKEN LKKWIGHPRF ELIRHDVTEP LLIEVDRIYH LACPASPIFY KYNPVKTIKT 130 140 150 160 170 180 NVIGTLNMLG LAKRVGARIL LTSTSEVYGD PLIHPQPESY WGNVNPIGVR SCYDEGKRVA 190 200 210 220 230 240 ETLMFDYHRQ HGIEIRIARI FNTYGPRMNI DDGRVVSNFI AQALRGEALT VQKPGTQTRS 250 260 270 280 290 300 FCYVSDMVDG LIRLMEGNDT GPINIGNPGE FTMVELAETV KELINPSIEI KMVENTPDDP 310 320 330 340 RQRKPDISKA KEVLGWEPKV KLREGLPLME EDFRLRLNVP RN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Biosynthesis of UDP-xylose. Cloning and characterization of a novel Arabidopsis gene family, UXS, encoding soluble and putative membrane-bound UDP-glucuronic acid decarboxylase isoforms." Harper A.D., Bar-Peled M. Plant Physiol. 130:2188-2198(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, FUNCTION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones." Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S. DNA Res. 5:379-391(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Biosynthesis of UDP-xylose: characterization of membrane-bound AtUxs2." Pattathil S., Harper A.D., Bar-Peled M. Planta 221:538-548(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[7]	"Reconstruction of de novo pathway for synthesis of UDP-glucuronic acid and UDP-xylose from intrinsic UDP-glucose in Saccharomyces cerevisiae." Oka T., Jigami Y. FEBS J. 273:2645-2657(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF387789 mRNA. Translation: AAK70882.1. AB016890 Genomic DNA. Translation: BAB09774.1. CP002688 Genomic DNA. Translation: AED97166.1. AF375442 mRNA. Translation: AAK53026.1. AY093958 mRNA. Translation: AAM16219.1. AY088443 mRNA. Translation: AAM65979.1.
IPI	IPI00542582. IPI00846930.
RefSeq	NP_200737.1. NM_125319.2.
UniGene	At.24136.
3D structure databases
ProteinModelPortal	Q9FIE8.
SMR	Q9FIE8. Positions 31-326.
ModBase	Search...
Proteomic databases
PaxDb	Q9FIE8.
PRIDE	Q9FIE8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G59290.1; AT5G59290.1; AT5G59290.
GeneID	836047.
KEGG	ath:AT5G59290.
Organism-specific databases
TAIR	At5g59290.
Phylogenomic databases
eggNOG	COG0451.
HOGENOM	HOG000168004.
InParanoid	Q9FIE8.
PhylomeDB	Q9FIE8.
ProtClustDB	CLSN2683686.
Enzyme and pathway databases
BioCyc	ARA:AT5G59290-MONOMER. MetaCyc:AT5G59290-MONOMER.
UniPathway	UPA00796; UER00771.
Gene expression databases
ArrayExpress	Q9FIE8.
Genevestigator	Q9FIE8.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR001509. Epimerase_deHydtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF01370. Epimerase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UXS3_ARATH

Accession

Primary (citable) accession number: Q9FIE8
Secondary accession number(s): Q94JQ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2013
Last sequence update:	March 1, 2001
Last modified:	May 29, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program